Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

6-pyruvoyl tetrahydrobiopterin synthase

Gene

Pts

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. 6-pyruvoyl tetrahydrobiopterin synthase (Pts)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23Zinc1
Active sitei42Proton acceptor1
Metal bindingi48Zinc1
Metal bindingi50Zinc1
Active sitei89Charge relay system1
Active sitei133Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • tetrahydrobiopterin biosynthetic process Source: RGD

Keywordsi

Molecular functionLyase
Biological processTetrahydrobiopterin biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi4.2.3.12 5301
ReactomeiR-RNO-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
UniPathwayiUPA00849; UER00819

Names & Taxonomyi

Protein namesi
Recommended name:
6-pyruvoyl tetrahydrobiopterin synthase (EC:4.2.3.12)
Short name:
PTP synthase
Short name:
PTPS
Gene namesi
Name:Pts
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi68367 Pts

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Involvement in diseasei

Deficiency leads to phenylketonuria.

Keywords - Diseasei

Phenylketonuria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000298681 – 44
ChainiPRO_00000298695 – 1446-pyruvoyl tetrahydrobiopterin synthaseAdd BLAST140

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei127PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-18 is required for maximal enzyme activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP27213
PRIDEiP27213

PTM databases

iPTMnetiP27213
PhosphoSitePlusiP27213

Expressioni

Gene expression databases

BgeeiENSRNOG00000009250
GenevisibleiP27213 RN

Interactioni

Subunit structurei

Homohexamer formed of two homotrimers in a head to head fashion.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012434

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 23Combined sources14
Helixi32 – 39Combined sources8
Helixi40 – 43Combined sources4
Beta strandi48 – 61Combined sources14
Turni64 – 66Combined sources3
Helixi72 – 82Combined sources11
Helixi84 – 87Combined sources4
Helixi92 – 95Combined sources4
Helixi97 – 99Combined sources3
Beta strandi100 – 102Combined sources3
Helixi106 – 120Combined sources15
Turni123 – 125Combined sources3
Beta strandi126 – 134Combined sources9
Beta strandi137 – 141Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B66X-ray1.90A/B5-144[»]
1B6ZX-ray2.00A/B5-144[»]
1GTQX-ray2.30A/B5-144[»]
ProteinModelPortaliP27213
SMRiP27213
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27213

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family.Curated

Phylogenomic databases

eggNOGiKOG4105 Eukaryota
COG0720 LUCA
GeneTreeiENSGT00390000002752
HOGENOMiHOG000225069
HOVERGENiHBG004358
InParanoidiP27213
KOiK01737
OMAiNIAVVIW
OrthoDBiEOG091G0TKH
PhylomeDBiP27213
TreeFamiTF105796

Family and domain databases

Gene3Di3.30.479.10, 1 hit
InterProiView protein in InterPro
IPR007115 6-PTP_synth/QueD
IPR038418 6-PTP_synth/QueD_sf
IPR022470 PTPS_Cys_AS
IPR022469 PTPS_His_AS
PANTHERiPTHR12589 PTHR12589, 1 hit
PfamiView protein in Pfam
PF01242 PTPS, 1 hit
PIRSFiPIRSF006113 PTP_synth, 1 hit
TIGRFAMsiTIGR00039 6PTHBS, 1 hit
PROSITEiView protein in PROSITE
PS00987 PTPS_1, 1 hit
PS00988 PTPS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAAVGLRRR ARLSRLVSFS ASHRLHSPSL SAEENLKVFG KCNNPNGHGH
60 70 80 90 100
NYKVVVTIHG EIDPVTGMVM NLTDLKEYME EAIMKPLDHK NLDLDVPYFA
110 120 130 140
DVVSTTENVA VYIWENLQRL LPVGALYKVK VYETDNNIVV YKGE
Length:144
Mass (Da):16,241
Last modified:August 1, 1992 - v1
Checksum:i0657C78F87C6FC3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77850 mRNA Translation: AAA40625.1
BC059140 mRNA Translation: AAH59140.1
PIRiA39499
RefSeqiNP_058916.1, NM_017220.1
UniGeneiRn.87164

Genome annotation databases

EnsembliENSRNOT00000012434; ENSRNOP00000012434; ENSRNOG00000009250
GeneIDi29498
KEGGirno:29498
UCSCiRGD:68367 rat

Similar proteinsi

Entry informationi

Entry nameiPTPS_RAT
AccessioniPrimary (citable) accession number: P27213
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health