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Protein

6-pyruvoyl tetrahydrobiopterin synthase

Gene

Pts

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. 6-pyruvoyl tetrahydrobiopterin synthase (Pts)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23Zinc1
Active sitei42Proton acceptor1
Metal bindingi48Zinc1
Metal bindingi50Zinc1
Active sitei89Charge relay system1
Active sitei133Charge relay system1

GO - Molecular functioni

  • 6-pyruvoyltetrahydropterin synthase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • tetrahydrobiopterin biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi4.2.3.12. 5301.
ReactomeiR-RNO-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00849; UER00819.

Names & Taxonomyi

Protein namesi
Recommended name:
6-pyruvoyl tetrahydrobiopterin synthase (EC:4.2.3.12)
Short name:
PTP synthase
Short name:
PTPS
Gene namesi
Name:Pts
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi68367. Pts.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Involvement in diseasei

Deficiency leads to phenylketonuria.

Keywords - Diseasei

Phenylketonuria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000298681 – 44
ChainiPRO_00000298695 – 1446-pyruvoyl tetrahydrobiopterin synthaseAdd BLAST140

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei127PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-18 is required for maximal enzyme activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP27213.
PRIDEiP27213.

PTM databases

iPTMnetiP27213.
PhosphoSitePlusiP27213.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009250.
GenevisibleiP27213. RN.

Interactioni

Subunit structurei

Homohexamer formed of two homotrimers in a head to head fashion.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012434.

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 23Combined sources14
Helixi32 – 39Combined sources8
Helixi40 – 43Combined sources4
Beta strandi48 – 61Combined sources14
Turni64 – 66Combined sources3
Helixi72 – 82Combined sources11
Helixi84 – 87Combined sources4
Helixi92 – 95Combined sources4
Helixi97 – 99Combined sources3
Beta strandi100 – 102Combined sources3
Helixi106 – 120Combined sources15
Turni123 – 125Combined sources3
Beta strandi126 – 134Combined sources9
Beta strandi137 – 141Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B66X-ray1.90A/B5-144[»]
1B6ZX-ray2.00A/B5-144[»]
1GTQX-ray2.30A/B5-144[»]
ProteinModelPortaliP27213.
SMRiP27213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27213.

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family.Curated

Phylogenomic databases

eggNOGiKOG4105. Eukaryota.
COG0720. LUCA.
GeneTreeiENSGT00390000002752.
HOGENOMiHOG000225069.
HOVERGENiHBG004358.
InParanoidiP27213.
KOiK01737.
OMAiHGHNYVW.
OrthoDBiEOG091G0TKH.
PhylomeDBiP27213.
TreeFamiTF105796.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
IPR022470. PTPS_Cys_AS.
IPR022469. PTPS_His_AS.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
PROSITEiPS00987. PTPS_1. 1 hit.
PS00988. PTPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAAVGLRRR ARLSRLVSFS ASHRLHSPSL SAEENLKVFG KCNNPNGHGH
60 70 80 90 100
NYKVVVTIHG EIDPVTGMVM NLTDLKEYME EAIMKPLDHK NLDLDVPYFA
110 120 130 140
DVVSTTENVA VYIWENLQRL LPVGALYKVK VYETDNNIVV YKGE
Length:144
Mass (Da):16,241
Last modified:August 1, 1992 - v1
Checksum:i0657C78F87C6FC3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77850 mRNA. Translation: AAA40625.1.
BC059140 mRNA. Translation: AAH59140.1.
PIRiA39499.
RefSeqiNP_058916.1. NM_017220.1.
UniGeneiRn.87164.

Genome annotation databases

EnsembliENSRNOT00000012434; ENSRNOP00000012434; ENSRNOG00000009250.
GeneIDi29498.
KEGGirno:29498.
UCSCiRGD:68367. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77850 mRNA. Translation: AAA40625.1.
BC059140 mRNA. Translation: AAH59140.1.
PIRiA39499.
RefSeqiNP_058916.1. NM_017220.1.
UniGeneiRn.87164.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B66X-ray1.90A/B5-144[»]
1B6ZX-ray2.00A/B5-144[»]
1GTQX-ray2.30A/B5-144[»]
ProteinModelPortaliP27213.
SMRiP27213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012434.

PTM databases

iPTMnetiP27213.
PhosphoSitePlusiP27213.

Proteomic databases

PaxDbiP27213.
PRIDEiP27213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012434; ENSRNOP00000012434; ENSRNOG00000009250.
GeneIDi29498.
KEGGirno:29498.
UCSCiRGD:68367. rat.

Organism-specific databases

CTDi5805.
RGDi68367. Pts.

Phylogenomic databases

eggNOGiKOG4105. Eukaryota.
COG0720. LUCA.
GeneTreeiENSGT00390000002752.
HOGENOMiHOG000225069.
HOVERGENiHBG004358.
InParanoidiP27213.
KOiK01737.
OMAiHGHNYVW.
OrthoDBiEOG091G0TKH.
PhylomeDBiP27213.
TreeFamiTF105796.

Enzyme and pathway databases

UniPathwayiUPA00849; UER00819.
BRENDAi4.2.3.12. 5301.
ReactomeiR-RNO-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

EvolutionaryTraceiP27213.
PROiP27213.

Gene expression databases

BgeeiENSRNOG00000009250.
GenevisibleiP27213. RN.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
IPR022470. PTPS_Cys_AS.
IPR022469. PTPS_His_AS.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
PROSITEiPS00987. PTPS_1. 1 hit.
PS00988. PTPS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPS_RAT
AccessioniPrimary (citable) accession number: P27213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.