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Protein

RNA-directed RNA polymerase

Gene

ORF1

Organism
Tobacco necrosis virus (strain D) (TNV-D)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase that plays an essential role in the virus replication.Curated

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein p82
Cleaved into the following chain:
Gene namesi
ORF Names:ORF1
OrganismiTobacco necrosis virus (strain D) (TNV-D)
Taxonomic identifieri12056 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageTombusviridaeBetanecrovirus
Virus hostiChenopodium amaranticolor [TaxID: 66262]
Chenopodium quinoa (Quinoa) [TaxID: 63459]
Cucumis sativus (Cucumber) [TaxID: 3659]
Nicotiana clevelandii (Wild tobacco) [TaxID: 81866]
Nicotiana tabacum (Common tobacco) [TaxID: 4097]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Tulipa gesneriana (Garden tulip) [TaxID: 13306]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725RNA-directed RNA polymerasePRO_0000040219Add
BLAST
Chaini1 – 202202Protein p23PRO_0000040220Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP27209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini424 – 544121RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
[Graphical view]
PfamiPF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
[Graphical view]
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27209-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLPIVLLS LISKAVVLIC SLLTLIIQNS TAVTWGLACV WLAYVSFRFL
60 70 80 90 100
FQVKITVHPA ARETFESMVR KFQAESMFSE EATPCIVSVG DKDADLTPDP
110 120 130 140 150
QREDIKIVKS SRRVSYAVRV AHVAKAQVGL LANSRANELV YSRLCREEMV
160 170 180 190 200
KHGVRPSHIA HMVPLAVAAC FIPLDSDFLA ASIRQGEGMR ERRALLGPSW
210 220 230 240 250
EKXGGLLVTS GFTTPTWRGD PRGMLVTKGP PLAKPRKLYR FTGMGTHIRY
260 270 280 290 300
GVHDHSLGNV RRGLVERLYM VEVKGELQPT PKPTPGAFNQ MSRFSDRLSI
310 320 330 340 350
HLPKTTRLTP REFLGFYTGR KLERYQKAVE SLEMHPVREK DAWLSTFVKA
360 370 380 390 400
EKLNITAKPD PAPRVIQPRD PRYNVEVGRF LRHSEEMLFK AINKTFGGRT
410 420 430 440 450
IFKGLSSDQA GEEFKTLWDS FKDPVGIGMD ASRFDQHISK DALEFEHKMW
460 470 480 490 500
LSMFPKSERA ELARLLSWQI NNRGLARCPD GEIRYRVEGC RMSGDMNTSS
510 520 530 540 550
GNCYIMCATV HNWCDNIKHI KHFRLANNGD DCMLVVERSD EKKVRNGLIE
560 570 580 590 600
YYATLGFTMK VEPTVDVLER LEFCQTRPVL VDGKYRMVRN LHQSMSKDLH
610 620 630 640 650
SLHDLDSSAA RNAWVTAVGT GGRCMNDGVP VLKEFFKQFP DYNLEVKKGS
660 670 680 690 700
DMAQKLRDDW KYKFNRTAAF QDLIPTQESR YSFWLAFGLL PDEQIALENG
710 720
FSPLKMEIVN EQIQEETSLL QFSGA
Length:725
Mass (Da):82,357
Last modified:October 5, 2010 - v3
Checksum:i5A57467158A30634
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00942 Genomic RNA. Translation: BAA00785.1.
D00942 Genomic RNA. Translation: BAA00786.1.
PIRiJU0368. RRWQTD.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00942 Genomic RNA. Translation: BAA00785.1.
D00942 Genomic RNA. Translation: BAA00786.1.
PIRiJU0368. RRWQTD.

3D structure databases

ProteinModelPortaliP27209.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
[Graphical view]
PfamiPF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
[Graphical view]
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete nucleotide sequence of tobacco necrosis virus strain D."
    Coutts R.H.A., Rigden J.E., Slabas A.R., Lomonossoff G.P., Wise P.J.
    J. Gen. Virol. 72:1521-1529(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Coutts R.H.A.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiRDRP_TNVD
AccessioniPrimary (citable) accession number: P27209
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 5, 2010
Last modified: June 24, 2015
This is version 65 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Readthrough of the terminator UAG occurs at position 203.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.