P27197 (AURB_CHLAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Auracyanin-B
Short name=Ac-B

Cleaved into the following 2 chains:

1. Auracyanin-B-1
2. Auracyanin-B-2

Gene names

Ordered Locus Names:

Caur_1950

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) [Reference proteome] [HAMAP]

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus › Chloroflexus aurantiacus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can donate electrons to cytochrome c-554. Ref.3
Cofactor	Binds 1 copper ion per subunit.
Subcellular location	Membrane; Peripheral membrane protein Ref.3.
Post-translational modification	Glycosylated. Ref.3
Sequence similarities	Belongs to the multicopper oxidase family. Contains 1 plastocyanin-like domain.
Biophysicochemical properties	Redox potential: E0 is +190 mV at pH 9, +215 mV at pH 7 and +240 mV at pH 4. Ref.4

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane
Domain	Signal
Ligand	Copper Metal-binding
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	photosynthetic electron transport chain Inferred from direct assay Ref.4. Source: UniProtKB
Cellular_component	plasma membrane Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	copper ion binding Inferred from direct assay Ref.3. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 56

56

Potential

Propeptide

57 – 80

24

PRO_0000002846

Chain

81 – 235

155

Auracyanin-B-1

PRO_0000002847

Chain

89 – 235

147

Auracyanin-B-2

PRO_0000002848

Regions

Domain

111 – 235

125

Plastocyanin-like

Sites

Metal binding

152

1

Copper

Metal binding

217

1

Copper

Metal binding

222

1

Copper

Metal binding

227

1

Copper

Experimental info

Sequence conflict

183

1

P → G AA sequence Ref.3

Sequence conflict

186

1

P → A AA sequence Ref.3

Sequence conflict

219

1

F → G AA sequence Ref.3

Sequence conflict

223 – 226

4

YLAG → TPI AA sequence Ref.3

Secondary structure

1

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235

Helix Strand Turn

Details...

Beta strand

111 – 118

8

Beta strand

120 – 126

7

Beta strand

128 – 133

6

Beta strand

137 – 144

8

Beta strand

155 – 160

6

Helix

161 – 172

12

Helix

175 – 177

3

Beta strand

188 – 191

4

Beta strand

199 – 206

8

Beta strand

209 – 216

8

Turn

220 – 226

7

Beta strand

228 – 234

7

Sequences

	Sequence		Length	Mass (Da)
	P27197 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 845186230C072521	FASTA	235	23,716
	10 20 30 40 50 60 MSWRGSGRSN FRSRSSSNGG STFSGGSAGG PPLIVMMGLA FGAGLIMLIV MIASNATAGG 70 80 90 100 110 120 FVAATPRPTA TPRPTAAPAP TQPPAAQPTT APATQAANAP GGSNVVNETP AQTVEVRAAP 130 140 150 160 170 180 DALAFAQTSL SLPANTVVRL DFVNQNNLGV QHNWVLVNGG DDVAAAVNTA AQNNADALFV 190 200 210 220 230 PPPDTPNALA WTAMLNAGES GSVTFRTPAP GTYLYICTFP GHYLAGMKGT LTVTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene sequence for auracyanin B from Chloroflexus aurantiacus." Lopez J.C., Dracheva S., Blankenship R.E. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Chloroflexus aurantiacus J-10-fl." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29366 / DSM 635 / J-10-fl.
[3]	"Isolation, characterization, and amino acid sequences of auracyanins, blue copper proteins from the green photosynthetic bacterium Chloroflexus aurantiacus." McManus J.D., Brune D.C., Han J., Sanders-Loehr J., Meyer T.E., Cusanovich M.A., Tollin G., Blankenship R.E. J. Biol. Chem. 267:6531-6540(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 81-235, FUNCTION, COPPER BINDING, SUBCELLULAR LOCATION, GLYCOSYLATION.
[4]	"A thin-film electrochemical study of the 'blue' copper proteins, auracyanin A and auracyanin B, from the photosynthetic bacterium Chloroflexus aurantiacus: the reduction potential as a function of pH." Rooney M.B., Honeychurch M.J., Selvaraj F.M., Blankenship R.E., Bond A.M., Freeman H.C. J. Biol. Inorg. Chem. 8:306-317(2003) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
[5]	"Crystal structure of auracyanin, a 'blue' copper protein from the green thermophilic photosynthetic bacterium Chloroflexus aurantiacus." Bond C.S., Blankenship R.E., Freeman H.C., Guss J.M., Maher M.J., Selvaraj F.M., Wilce M.C.J., Willingham K.M. J. Mol. Biol. 306:47-67(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 81-235.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U78046 Genomic DNA. Translation: AAB38318.1.
CP000909 Genomic DNA. Translation: ABY35165.1.

RefSeq

YP_001635554.1. NC_010175.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OV8	X-ray	1.90	A/B/C/D	96-235	[»]
1QHQ	X-ray	1.55	A	96-235	[»]

ProteinModelPortal

P27197.

SMR

P27197. Positions 97-235.

ModBase

Search...

Protein-protein interaction databases

STRING

324602.Caur_1950.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

ABY35165; ABY35165; Caur_1950.

GeneID

5826401.

KEGG

cau:Caur_1950.

PATRIC

21414911. VBIChlAur28763_2219.

Phylogenomic databases

eggNOG

NOG285099.

HOGENOM

HOG000034233.

OMA

HYLAGMK.

ProtClustDB

CLSK2477013.

Enzyme and pathway databases

BioCyc

CAUR324602:GIXU-1976-MONOMER.

Family and domain databases

Gene3D

2.60.40.420. 1 hit.

InterPro

IPR000923. BlueCu_1.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]

Pfam

PF00127. Copper-bind. 1 hit.
[Graphical view]

SUPFAM

SSF49503. Cupredoxin. 1 hit.

PROSITE

PS00196. COPPER_BLUE. 1 hit.
PS00079. MULTICOPPER_OXIDASE1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P27197.

Entry information

Entry name

AURB_CHLAA

Accession

Primary (citable) accession number: P27197
Secondary accession number(s): A9WE06, P94610

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program