Reviewed, UniProtKB/Swiss-Prot P27195 (PPCF_ELIMR)

Names and origin

Protein names	Recommended name:     Prolyl endopeptidase     EC=3.4.21.26 Alternative name(s):     Proline-specific endopeptidase       Short name=PSE       Short name=PE     Post-proline cleaving enzyme
Organism	Elizabethkingia miricola (Chryseobacterium miricola)
Taxonomic identifier	172045 [NCBI]
Taxonomic lineage	Bacteria › Bacteroidetes › Flavobacteria › Flavobacteriales › Flavobacteriaceae › Elizabethkingia

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has an absolute requirement for an X-Pro bond in the trans configuration immediately preceding the Pro-Y scissible bond.
Catalytic activity	Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.
Subunit structure	Monomer.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the peptidase S9A family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 705	685	Prolyl endopeptidase		PRO_0000027208
Sites
	Active site	556	1	Charge relay system
	Active site	675	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27195-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 36925D9A8783E03A	FASTA	705	78,790
	10 20 30 40 50 60 MKYKKLSVAV AAFAFAAVSA QNSNSLKYPE TKKVNHTDTY FGNQVSDPYR WLEDDRAEDT 70 80 90 100 110 120 KAWVQQEVKF TQDYLAQIPF RGQIKKQLLD IWNYEKISAP FKKGKYTYFY KNDGLQAQSV 130 140 150 160 170 180 LYRKDASGKT EVFLDPNKFS DKGTTSLANL SFNKKGTLVA YSISEGGSDW NKIIILDAET 190 200 210 220 230 240 KKQIDETLLD VKFSGISWLG DEGFFYSSYD KPKDGSVLSG MTDKHKVYFH KLGTKQSQDE 250 260 270 280 290 300 LIIGGDKFPR RYLSGYVTED QRYLVVSAAN ATNGNELYIK DLKNKTDFIP IITGFESNVG 310 320 330 340 350 360 LVDTDGDTLF LHTDKNAPNM RMVKTTIQNP KPETWKDVIA ETSEPMRVNS GGGYFFATYM 370 380 390 400 410 420 KDALSQIKQY DKTGKLVREI KLPGSGTAGG FGGEKTEKEL YYSFTNYITP PTIFKFSIDS 430 440 450 460 470 480 GKSEVYQKPK VKFNPENYVS EQVFYTSADG TKIPMMISNK KGLKKDGKNP TILYSYGGFN 490 500 510 520 530 540 ISLQPAFSVV NAIWMENGGI YAVPNIRGGG EYGKKWHDAG TKQQKKNVFN DFIAAGEYLQ 550 560 570 580 590 600 KNGYTSKDYM ALSGRSNGGL LVGATMTMRP DLAKVAFPGV GVLDMLRYNK FTAGAGWAYD 610 620 630 640 650 660 YGTAEDSKEM FEYLKSYSPV HNVKAGTCYP STMVITSDHD DRVVPAHSFK FGAELQAKQA 670 680 690 700 CKNPVLIRIE TNAGHGAGRS TEQVVMENAD LLSFALYEMG IKNLK

References

[1]

"Characterization of a prolyl endopeptidase from Flavobacterium meningosepticum. Complete sequence and localization of the active-site serine." Chevallier S., Goeltz P., Thibault P., Banville D., Gagnon J. J. Biol. Chem. 267:8192-8199(1992) [PubMed: 1569074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 33958.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M81461 Genomic DNA. Translation: AAA24925.1.
3D structure databases
HSSP	HSSP built from PDB template 1O6G based on UniProtKB P23687.
ModBase	Search...
Protein family/group databases
MEROPS	S09.001.
Family and domain databases
InterPro	IPR002471. Pept_S9_AS. IPR001375. Peptidase_S9. IPR002470. Peptidase_S9A. IPR004106. Peptidase_S9A_N. [Graphical view]
PANTHER	PTHR11757. Peptidase_S9A. 1 hit.
Pfam	PF00326. Peptidase_S9. 1 hit. PF02897. Peptidase_S9_N. 1 hit. [Graphical view]
PRINTS	PR00862. PROLIGOPTASE.
PROSITE	PS00708. PRO_ENDOPEP_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPCF_ELIMR

Accession

Primary (citable) accession number: P27195

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)