P27176 (L_PUUMH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Puumala virus (strain Hallnas B1) (Nephropathia epidemica virus) [Complete proteome]

Taxonomic identifier

11605 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Hantavirus › Puumala virus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]

Protein attributes

Sequence length	2156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the hantavirus RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Cap snatching Viral RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2156	2156	RNA-directed RNA polymerase L		PRO_0000222022
Regions
	Domain	956 – 1142	187	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P27176 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 1D3C0A34C0DF99A6	FASTA	2,156	247,050
	10 20 30 40 50 60 MEKYREIHER VKEAVPGETS AVECLDLLDR LYAVRHDVVD QMIKHDWSDN KDKEQPIGLV 70 80 90 100 110 120 LLMAGVPNDV IQSMEKRIIP GSPSGQILRS FFKMTPDNYK ITGNLIEFIE VTVTADVARG 130 140 150 160 170 180 VREKILKYQG GLEFIEQLLQ IEAQKGNCQS GFRIKFDVVA IRTDGSNIST QWPSRRNEGV 190 200 210 220 230 240 VQAMRLIQAD INFVREHLIK NDERGALEAM FNLKFHVTGP KVRTFDIPNY RPQQLCQPVL 250 260 270 280 290 300 ENLVEYCKNW LGTDHAFAFK EVTGQRVFNV FRDEEELHAS KYGHSRKPRN FLLCQISLQV 310 320 330 340 350 360 PYLPSTIASD QYDTRLACSE ILKNYPETPL QLLARDMAYK YITLDNEDII NYYNPRVYFK 370 380 390 400 410 420 PTQNIKEPGT FKLNLSNMDP KSKALIDVIS KDSKKGVFGE LIDSIDVASQ VQQNECAKTI 430 440 450 460 470 480 EKILSDLEVN LGDSTAGLDQ PKRTTGVDDI LRKFYDNELV KYLISVIRKT TARHLGHLLR 490 500 510 520 530 540 DITESLIAHA GLKRSKYWSA HGYAYGSVLL CILPSKSLEV AGSFIRFFTV FKEGLGLIDA 550 560 570 580 590 600 DNLDSKVEID GVTWCFSKII SLDLNRLLAL NIAFEKSLLA TATWFQYYTE DQGHFPLQHA 610 620 630 640 650 660 LRSVFAFHFL LATSQKMKLC AIFDNLRYLI PAVTSTYSGF EPLIRKFFER PFKSALEVYL 670 680 690 700 710 720 YNIIKTLLVS LAQNNKIRFY SRVRLLGLTV DQSSIGASGV YPSLMSRVVY KHYRSLISEA 730 740 750 760 770 780 TTCFFLFEKG LHGNLTEEAK IHLETVEWAR KFREKERKLG SYIMEEGYHI QDVLNNQVVV 790 800 810 820 830 840 EQQLFCQEVV ELAAQELNTY LHAKSQVMAS NIMNKHWDKP YFSQTRNISL KGMSGALQED 850 860 870 880 890 900 GHLAASVTLI EAIRFLNHSQ NNPTVLELYE QTKKQKAQAR IVRKYQRTEA DRGFFITTLP 910 920 930 940 950 960 TRVRLEIIED YYDAIARVVP EEYISYGGET KILNIQQALE KALRWASGES EIQSSIGHSI 970 980 990 1000 1010 1020 KLKRKLMYVS ADATKWSPGD NSAKFRRFTQ SLYDGLRDDK LKNCVVDALR NIYETDFFIS 1030 1040 1050 1060 1070 1080 RKLHRYIDNM GELSDEVLDF LSFFPNKVSA SIKGNWLQGN LNKCSSLFGA AVSLLFKRVW 1090 1100 1110 1120 1130 1140 AKLYPELECF FEFAHHSDDA LFIYGYLEPV DDGTEWFQYV TQQIQAGNFH WHAVNQEMWK 1150 1160 1170 1180 1190 1200 SMFNLHEHIL LMGSIKISPK KTTVSPTNAE FLSTFFEGCA VSIPFIKILL GSLSDLPGLG 1210 1220 1230 1240 1250 1260 YFDDLAAAQS RCVKALDMGA CPQLAQLGIV LCTSKVERLY GTAPGMVNNP TAYLKVDRSL 1270 1280 1290 1300 1310 1320 IPIPLGGDGS MSIMELATAG IGMADKNILK NAFITYKHAK KDNDRYVLGL FKFLMSLSDD 1330 1340 1350 1360 1370 1380 IFQHDRLGEF SFVGKVQWKV FTPKSEFEFY DQYSRKYLEL WSEQHPVYDY IIPRGRDNLL 1390 1400 1410 1420 1430 1440 VYLVRKLNDP SIVTAMTMQS PLQLRFRMQA KQHMKVCKLD GEWVTFREVL AAADAFASEY 1450 1460 1470 1480 1490 1500 RPTLQDMELF QTLVNCTFSK EYAWRDFLNE VQCDVLTTRQ IHRPKVARTF TVKEKDQTIQ 1510 1520 1530 1540 1550 1560 NPITAVIGYK YASKVDEISD VLDSAIHPDS LSTDLQLMRE GVYRELGLDI SQPNVLKKVA 1570 1580 1590 1600 1610 1620 PLLYKSGKSR IVIVQGNVEG TAESICSYWL KTMSLVKTIK VKPKKEVLKA VSLYGKKEKA 1630 1640 1650 1660 1670 1680 GDLTHLAAMR LCIEVWRWCK ANEQDSVSWL KYLMFENKTL EQWIDSFCSR GVLPVDPEIQ 1690 1700 1710 1720 1730 1740 CLGLLVYDLK GQKGLLQIQA NRRAYSGKQY DAYCVQTYNE ETKLYEGDLR VTFNFGIDCA 1750 1760 1770 1780 1790 1800 RLEIFWDKRE YILETSITQR NVLKILMEEV TKELLRCGMR FKTEQVNSSR SVVLFKTESG 1810 1820 1830 1840 1850 1860 FEWGKPNVPC IVYRNCTLRT GLRVRQPTNK AFSITIQANG FRAMAQLDEE NPRFLLAHAY 1870 1880 1890 1900 1910 1920 HNLKDVRYQA LQAVGNVWFK MTQHKLFINP IISAGLLENF MKGLPAAIPP AAYSLIMNKA 1930 1940 1950 1960 1970 1980 KISVDLFMFN ELLALINPQN VLNLDGIEET SEGYTTVSTI SSTQWSEEVS LTMDDSDDDG 1990 2000 2010 2020 2030 2040 DASQLDYTID LDDIDFETID LKEDIEHFLQ DESAYTGDLL IQTEETEIRK LRGMIKILEP 2050 2060 2070 2080 2090 2100 VKLIKSWVSK GLSIDKIYNP VNIILMTRYM SKHYNFHAKQ LSLMDPYDLT EFESIVKGWG 2110 2120 2130 2140 2150 ECVKDRFIEL DQEAQRKVTE ERVLPEDVLP DSLFSFRHAD ILLKRLFPRD SASSFY

References

[1]	"Primary structure of the large (L) RNA segment of nephropathia epidemica virus strain Hallnas B1 coding for the viral RNA polymerase." Stohwasser R., Raab K., Darai G., Bautz E.K.F. Virology 183:386-391(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63194 Genomic RNA. No translation available.
PIR	RRVUNE. A40319.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR026382. CapSnatchArena. IPR016268. RNA-dir_pol_hantavirus. IPR024378. RNA-dir_pol_N_hantavirus. IPR007099. RNA-dir_pol_NSvirus. IPR007322. RNA_pol_bunyavir. [Graphical view]
Pfam	PF04196. Bunya_RdRp. 1 hit. PF12426. DUF3674. 1 hit. [Graphical view]
PIRSF	PIRSF000825. L_HantaV. 1 hit.
TIGRFAMs	TIGR04202. capSnatchArena. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_PUUMH

Accession

Primary (citable) accession number: P27176

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program