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Reviewed, UniProtKB/Swiss-Prot P27175 (DHG_GLUOX)

Last modified June 16, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Quinoprotein glucose dehydrogenase
    EC=1.1.5.2
Alternative name(s):
    Glucose dehydrogenase [pyrroloquinoline-quinone]
Gene names
Name: gdh
Ordered Locus Names: GOX0265
OrganismGluconobacter oxydans (Gluconobacter suboxydans) [Complete proteome] [HAMAP]
Taxonomic identifier442 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

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Protein attributes

Sequence length808 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol.

Cofactor

PQQ.

Subcellular location

Cell inner membrane; Multi-pass membrane protein; Periplasmic side.

Miscellaneous

The P1 form can oxidize only D-glucose, while the P2 form can also oxidize maltose. The sequence of P1 form is shown here.

Sequence similarities

Belongs to the bacterial PQQ dehydrogenase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 808775Quinoprotein glucose dehydrogenase
PRO_0000025558

Regions

Transmembrane35 – 5420 Potential
Transmembrane59 – 7618 Potential
Transmembrane94 – 10815 Potential
Transmembrane123 – 13816 Potential

Sites

Active site4701Proton acceptor Potential

Natural variations

Natural variant7881H → N in P2 form.

Experimental info

Sequence conflict41T → I in CAA44594. Ref.1
Sequence conflict81G → R in CAA44594. Ref.1
Sequence conflict110 – 12314QIGGT…LPLAG → ARSVARGRPSCRSP Ref.1
Sequence conflict1461D → E in CAA44594. Ref.1
Sequence conflict1881S → T in CAA44594. Ref.1
Sequence conflict2131A → Q Ref.1
Sequence conflict2771T → M in CAA44594. Ref.1
Sequence conflict291 – 2922RI → DS in CAA44594. Ref.1
Sequence conflict3951D → E in CAA44594. Ref.1
Sequence conflict4151R → A in CAA44594. Ref.1
Sequence conflict472 – 4732DV → EL in CAA44594. Ref.1
Sequence conflict635 – 6362KD → EN in CAA44594. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P27175-1 [UniParc].

Last modified March 15, 2005. Version 2.
Checksum: 6FFF304215A63D29

FASTA80887,335
        10         20         30         40         50         60 
MSTTSRPGLW ALITAAVFAL CGAILTVGGA WVAAIGGPLY YVILGLALLA TAFLSFRRNP 

        70         80         90        100        110        120 
AALYLFAVVV FGTVIWELTV VGLDIWALIP RSDIVIILGI WLLLPFVSRQ IGGTRTTVLP 

       130        140        150        160        170        180 
LAGAVGVAVL ALFASLFTDP HDISGDLPTQ IANASPADPD NVPASEWHAY GRTQAGDRWS 

       190        200        210        220        230        240 
PLNQINASNV SNLKVAWHIH TKDMMNSNDP GEATNEATPI EFNNTLYMCS LHQKLFAVDG 

       250        260        270        280        290        300 
ATGNVKWVYD PKLQINPGFQ HLTCRGVSFH ETPANATDSD GNPAPTDCAK RIILPVNDGR 

       310        320        330        340        350        360 
LVEVDADTGK TCSGFGNNGE IDLRVPNQPY TTPGQYEPTS PPVITDKLII ANSAITDNGS 

       370        380        390        400        410        420 
VKQASGATQA FDVYTGKRVW VFDASNPDPN QLPDDSHPVF HPNSPNSWIV SSYDRNLNLV 

       430        440        450        460        470        480 
YIPMGVGTPD QWGGDRTKDS ERFAPGIVAL NADTGKLAWF YQTVHHDLWD MDVPSQPSLV 

       490        500        510        520        530        540 
DVTQKDGTLV PAIYAPTKTG DIFVLDRRTG KEIVPAPETP VPQGAAPGDH TSPTQPMSQL 

       550        560        570        580        590        600 
TLRPKNPLND SDIWGGTIFD QMFCSIYFHT LRYEGPFTPP SLKGSLIFPG DLGMFEWGGL 

       610        620        630        640        650        660 
AVDPQRQVAF ANPISLPFVS QLVPRGPGNP LWPEKDAKGT GGETGLQHNY GIPYAVNLHP 

       670        680        690        700        710        720 
FLDPVLLPFG IKMPCRTPPW GYVAGIDLKT NKVVWQHRNG TLRDSMYGSS LPIPLPPIKI 

       730        740        750        760        770        780 
GVPSLGGPLS TAGNLGFLTA SMDYYIRAYN LTTGKVLWQD RLPAGAQATP ITYAINGKQY 

       790        800 
IVTYAGGHNS FPTRMGDDII AYALPDQK

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References

« Hide 'large scale' references
[1]"A single amino acid substitution changes the substrate specificity of quinoprotein glucose dehydrogenase in Gluconobacter oxydans."
Cleton-Jansen A.-M., Dekker S., van de Putte P., Goosen N.
Mol. Gen. Genet. 229:206-212(1991) [PubMed: 1833618] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Goosen N.
Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 213.
[3]"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans."
Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.
Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 621H.

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Cross-references

Sequence databases

X62710 Genomic DNA. Translation: CAA44594.1. Sequence problems.
CP000009 Genomic DNA. Translation: AAW60048.1.
PIRQPKEX. S17716.
RefSeqYP_190704.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3248643.
GenomeReviewsGene locus GOX0265 in contig CP000009_GR.
KEGGgox:GOX0265.
NMPDRfig|290633.1.peg.249.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP27175.
OMAP27175. LTGADMW.

Enzyme and pathway databases

BioCycGOXY290633:GOX0265-MON.
BRENDA1.1.5.2. 3050.

Family and domain databases

InterProIPR017511. PQQ-dep_membr-bd_DH.
IPR019556. PQQ-dependent_C.
IPR019551. PQQ-dependent_N.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR011047. Quino_AlcDH-like.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
Gene3DG3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.
PfamPF01011. PQQ. 6 hits.
PF10535. PQQ_C. 1 hit.
PF10527. PQQ_N. 1 hit.
[Graphical view]
SMARTSM00564. PQQ. 3 hits.
[Graphical view]
TIGRFAMsTIGR03074. PQQ_membr_DH. 1 hit.
PROSITEPS00363. BACTERIAL_PQQ_1. 1 hit.
PS00364. BACTERIAL_PQQ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDHG_GLUOX
AccessionPrimary (citable) accession number: P27175
Secondary accession number(s): Q5FU98
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: March 15, 2005
Last modified: June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents