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P27158

- KITH_RAT

UniProt

P27158 - KITH_RAT

Protein

Thymidine kinase, cytosolic

Gene

Tk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei98 – 981Proton acceptorSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 338ATPCurated
    Nucleotide bindingi58 – 603ATPBy similarity
    Nucleotide bindingi97 – 1004ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. thymidine kinase activity Source: UniProtKB-EC
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. digestive tract development Source: RGD
    2. DNA replication Source: UniProtKB-KW
    3. fetal process involved in parturition Source: RGD
    4. liver development Source: RGD
    5. protein homotetramerization Source: Ensembl
    6. response to copper ion Source: RGD
    7. response to cortisol Source: RGD
    8. response to nutrient levels Source: RGD
    9. response to toxic substance Source: RGD
    10. skeletal muscle cell proliferation Source: RGD
    11. thymidine metabolic process Source: RGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_221948. Pyrimidine salvage reactions.
    SABIO-RKP27158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase, cytosolic (EC:2.7.1.21)
    Gene namesi
    Name:Tk1
    Synonyms:Tk-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621014. Tk1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – ›121›120Thymidine kinase, cytosolicPRO_0000174950Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei13 – 131PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on Ser-13 in mitosis.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP27158.

    Expressioni

    Gene expression databases

    GenevestigatoriP27158.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-8306437,EBI-8306437

    Protein-protein interaction databases

    MINTiMINT-8411768.

    Structurei

    3D structure databases

    ProteinModelPortaliP27158.
    SMRiP27158. Positions 18-121.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    HOVERGENiHBG006215.
    KOiK00857.
    PhylomeDBiP27158.

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27158-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYINLPTVL PISPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC    50
    LVIKYAKDTR YSNSFSTHDR NTMDALPACM LKDVAQEALG VAVIGIDEGQ 100
    FFPDIVDFCE TMANTGKTVI V 121
    Length:121
    Mass (Da):13,511
    Last modified:August 1, 1992 - v1
    Checksum:i8330BDFD9CDA9EB0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei121 – 1211

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22642 mRNA. Translation: AAA75560.1.
    Y17296 Genomic DNA. Translation: CAA76733.1.
    X54173 Genomic DNA. Translation: CAA38106.1.
    PIRiA39968.
    RefSeqiNP_434687.1. NM_052800.1.
    UniGeneiRn.147549.
    Rn.195325.
    Rn.233805.

    Genome annotation databases

    GeneIDi24834.
    KEGGirno:24834.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22642 mRNA. Translation: AAA75560.1 .
    Y17296 Genomic DNA. Translation: CAA76733.1 .
    X54173 Genomic DNA. Translation: CAA38106.1 .
    PIRi A39968.
    RefSeqi NP_434687.1. NM_052800.1.
    UniGenei Rn.147549.
    Rn.195325.
    Rn.233805.

    3D structure databases

    ProteinModelPortali P27158.
    SMRi P27158. Positions 18-121.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8411768.

    Chemistry

    BindingDBi P27158.
    ChEMBLi CHEMBL4022.

    Proteomic databases

    PRIDEi P27158.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24834.
    KEGGi rno:24834.

    Organism-specific databases

    CTDi 7083.
    RGDi 621014. Tk1.

    Phylogenomic databases

    HOVERGENi HBG006215.
    KOi K00857.
    PhylomeDBi P27158.

    Enzyme and pathway databases

    Reactomei REACT_221948. Pyrimidine salvage reactions.
    SABIO-RK P27158.

    Miscellaneous databases

    NextBioi 604568.
    PROi P27158.

    Gene expression databases

    Genevestigatori P27158.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning vectors for expression of cDNA libraries in mammalian cells."
      Murphy A.J.M., Efstratiadis A.
      Proc. Natl. Acad. Sci. U.S.A. 84:8277-8281(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Sauer M.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44.
      Strain: Sprague-Dawley.
    3. "The rat thymidine kinase gene 5' region: evolution of a promoter."
      Arcot S., Traina-Dorge V.L., Deininger P.L.
      DNA Seq. 2:129-131(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
      Tissue: Liver.

    Entry informationi

    Entry nameiKITH_RAT
    AccessioniPrimary (citable) accession number: P27158
    Secondary accession number(s): Q6LAG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3