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Protein

Thymidine kinase, cytosolic

Gene

Tk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Proton acceptorSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338ATPCurated
Nucleotide bindingi58 – 603ATPBy similarity
Nucleotide bindingi97 – 1004ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • thymidine kinase activity Source: UniProtKB-EC
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • digestive tract development Source: RGD
  • DNA biosynthetic process Source: UniProtKB-KW
  • fetal process involved in parturition Source: RGD
  • liver development Source: RGD
  • response to copper ion Source: RGD
  • response to cortisol Source: RGD
  • response to nutrient levels Source: RGD
  • response to toxic substance Source: RGD
  • skeletal muscle cell proliferation Source: RGD
  • thymidine metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.21. 5301.
ReactomeiREACT_353338. Pyrimidine salvage reactions.
SABIO-RKP27158.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:Tk1
Synonyms:Tk-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621014. Tk1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – ›121›120Thymidine kinase, cytosolicPRO_0000174950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP27158.

Expressioni

Gene expression databases

ExpressionAtlasiP27158. baseline and differential.
GenevisibleiP27158. RN.

Interactioni

Subunit structurei

Homotetramer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8306437,EBI-8306437

Protein-protein interaction databases

MINTiMINT-8411768.
STRINGi10116.ENSRNOP00000067459.

Structurei

3D structure databases

ProteinModelPortaliP27158.
SMRiP27158. Positions 18-121.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

HOVERGENiHBG006215.
InParanoidiP27158.
KOiK00857.
PhylomeDBiP27158.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYINLPTVL PISPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC
60 70 80 90 100
LVIKYAKDTR YSNSFSTHDR NTMDALPACM LKDVAQEALG VAVIGIDEGQ
110 120
FFPDIVDFCE TMANTGKTVI V
Length:121
Mass (Da):13,511
Last modified:August 1, 1992 - v1
Checksum:i8330BDFD9CDA9EB0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei121 – 1211

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22642 mRNA. Translation: AAA75560.1.
Y17296 Genomic DNA. Translation: CAA76733.1.
X54173 Genomic DNA. Translation: CAA38106.1.
PIRiA39968.
RefSeqiNP_434687.1. NM_052800.1.
UniGeneiRn.147549.
Rn.195325.
Rn.233805.

Genome annotation databases

GeneIDi24834.
KEGGirno:24834.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22642 mRNA. Translation: AAA75560.1.
Y17296 Genomic DNA. Translation: CAA76733.1.
X54173 Genomic DNA. Translation: CAA38106.1.
PIRiA39968.
RefSeqiNP_434687.1. NM_052800.1.
UniGeneiRn.147549.
Rn.195325.
Rn.233805.

3D structure databases

ProteinModelPortaliP27158.
SMRiP27158. Positions 18-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8411768.
STRINGi10116.ENSRNOP00000067459.

Chemistry

BindingDBiP27158.
ChEMBLiCHEMBL4022.

Proteomic databases

PRIDEiP27158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24834.
KEGGirno:24834.

Organism-specific databases

CTDi7083.
RGDi621014. Tk1.

Phylogenomic databases

HOVERGENiHBG006215.
InParanoidiP27158.
KOiK00857.
PhylomeDBiP27158.

Enzyme and pathway databases

BRENDAi2.7.1.21. 5301.
ReactomeiREACT_353338. Pyrimidine salvage reactions.
SABIO-RKP27158.

Miscellaneous databases

NextBioi604568.
PROiP27158.

Gene expression databases

ExpressionAtlasiP27158. baseline and differential.
GenevisibleiP27158. RN.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning vectors for expression of cDNA libraries in mammalian cells."
    Murphy A.J.M., Efstratiadis A.
    Proc. Natl. Acad. Sci. U.S.A. 84:8277-8281(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Sauer M.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44.
    Strain: Sprague-Dawley.
  3. "The rat thymidine kinase gene 5' region: evolution of a promoter."
    Arcot S., Traina-Dorge V.L., Deininger P.L.
    DNA Seq. 2:129-131(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
    Tissue: Liver.

Entry informationi

Entry nameiKITH_RAT
AccessioniPrimary (citable) accession number: P27158
Secondary accession number(s): Q6LAG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 22, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.