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P27158 (KITH_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase, cytosolic

EC=2.7.1.21
Gene names
Name:Tk1
Synonyms:Tk-1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length121 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Post-translational modification

Phosphorylated on Ser-13 in mitosis By similarity.

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Sequence similarities

Belongs to the thymidine kinase family.

Ontologies

Keywords
   Biological processDNA synthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

digestive tract development

Inferred from expression pattern PubMed 6701043. Source: RGD

fetal process involved in parturition

Inferred from expression pattern PubMed 7208144. Source: RGD

liver development

Inferred from expression pattern PubMed 995499. Source: RGD

protein homotetramerization

Inferred from electronic annotation. Source: Ensembl

response to copper ion

Inferred from expression pattern PubMed 6642140. Source: RGD

response to cortisol

Inferred from expression pattern PubMed 7208144. Source: RGD

response to nutrient levels

Inferred from expression pattern PubMed 6701043. Source: RGD

response to toxic substance

Inferred from expression pattern PubMed 6642140. Source: RGD

skeletal muscle cell proliferation

Inferred from expression pattern PubMed 15748706. Source: RGD

thymidine metabolic process

Inferred from direct assay PubMed 6642140. Source: RGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

identical protein binding

Inferred from physical interaction PubMed 23351158. Source: IntAct

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-8306437,EBI-8306437

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – ›121›120Thymidine kinase, cytosolic
PRO_0000174950

Regions

Nucleotide binding26 – 338ATP Probable
Nucleotide binding58 – 603ATP By similarity
Nucleotide binding97 – 1004ATP By similarity

Sites

Active site981Proton acceptor Potential

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue131Phosphoserine By similarity

Experimental info

Non-terminal residue1211

Sequences

Sequence LengthMass (Da)Tools
P27158 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 8330BDFD9CDA9EB0

FASTA12113,511
        10         20         30         40         50         60 
MSYINLPTVL PISPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC LVIKYAKDTR 

        70         80         90        100        110        120 
YSNSFSTHDR NTMDALPACM LKDVAQEALG VAVIGIDEGQ FFPDIVDFCE TMANTGKTVI 


V 

« Hide

References

[1]"Cloning vectors for expression of cDNA libraries in mammalian cells."
Murphy A.J.M., Efstratiadis A.
Proc. Natl. Acad. Sci. U.S.A. 84:8277-8281(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Sauer M.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44.
Strain: Sprague-Dawley.
[3]"The rat thymidine kinase gene 5' region: evolution of a promoter."
Arcot S., Traina-Dorge V.L., Deininger P.L.
DNA Seq. 2:129-131(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22642 mRNA. Translation: AAA75560.1.
Y17296 Genomic DNA. Translation: CAA76733.1.
X54173 Genomic DNA. Translation: CAA38106.1.
PIRA39968.
RefSeqNP_434687.1. NM_052800.1.
UniGeneRn.147549.
Rn.195325.
Rn.233805.

3D structure databases

ProteinModelPortalP27158.
SMRP27158. Positions 18-121.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8411768.

Chemistry

BindingDBP27158.
ChEMBLCHEMBL4022.

Proteomic databases

PRIDEP27158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24834.
KEGGrno:24834.

Organism-specific databases

CTD7083.
RGD621014. Tk1.

Phylogenomic databases

HOVERGENHBG006215.
KOK00857.
PhylomeDBP27158.

Enzyme and pathway databases

SABIO-RKP27158.

Gene expression databases

GenevestigatorP27158.

Family and domain databases

InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

NextBio604568.
PROP27158.

Entry information

Entry nameKITH_RAT
AccessionPrimary (citable) accession number: P27158
Secondary accession number(s): Q6LAG4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families