P27158 (KITH_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase, cytosolic
EC=2.7.1.21

Gene names

Name:	Tk1
Synonyms:	Tk-1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	121 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	Phosphorylated on Ser-13 in mitosis By similarity.
Miscellaneous	Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW digestive tract development Inferred from expression pattern PubMed 6701043. Source: RGD fetal process involved in parturition Inferred from expression pattern PubMed 7208144. Source: RGD liver development Inferred from expression pattern PubMed 995499. Source: RGD protein homotetramerization Inferred from electronic annotation. Source: Compara response to copper ion Inferred from expression pattern PubMed 6642140. Source: RGD response to cortisol stimulus Inferred from expression pattern PubMed 7208144. Source: RGD response to nutrient levels Inferred from expression pattern PubMed 6701043. Source: RGD response to toxin Inferred from expression pattern PubMed 6642140. Source: RGD skeletal muscle cell proliferation Inferred from expression pattern PubMed 15748706. Source: RGD thymidine metabolic process Inferred from direct assay PubMed 6642140. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – ›121	›120	Thymidine kinase, cytosolic		PRO_0000174950
Regions
	Nucleotide binding	26 – 33	8	ATP Probable
	Nucleotide binding	58 – 60	3	ATP By similarity
	Nucleotide binding	97 – 100	4	ATP By similarity
Sites
	Active site	98	1	Proton acceptor Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	13	1	Phosphoserine By similarity
Experimental info
	Non-terminal residue	121	1

Sequences

	Sequence		Length	Mass (Da)
	P27158 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 8330BDFD9CDA9EB0	FASTA	121	13,511
	10 20 30 40 50 60 MSYINLPTVL PISPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC LVIKYAKDTR 70 80 90 100 110 120 YSNSFSTHDR NTMDALPACM LKDVAQEALG VAVIGIDEGQ FFPDIVDFCE TMANTGKTVI V

References

[1]	"Cloning vectors for expression of cDNA libraries in mammalian cells." Murphy A.J.M., Efstratiadis A. Proc. Natl. Acad. Sci. U.S.A. 84:8277-8281(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Sauer M. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44. Strain: Sprague-Dawley.
[3]	"The rat thymidine kinase gene 5' region: evolution of a promoter." Arcot S., Traina-Dorge V.L., Deininger P.L. DNA Seq. 2:129-131(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22642 mRNA. Translation: AAA75560.1. Y17296 Genomic DNA. Translation: CAA76733.1. X54173 Genomic DNA. Translation: CAA38106.1.
IPI	IPI00194130.
PIR	A39968.
RefSeq	NP_434687.1. NM_052800.1.
UniGene	Rn.147549. Rn.195325. Rn.216486.
3D structure databases
ProteinModelPortal	P27158.
SMR	P27158. Positions 18-121.
ModBase	Search...
Proteomic databases
PRIDE	P27158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24834.
KEGG	rno:24834.
Organism-specific databases
CTD	7083.
RGD	621014. Tk1.
Phylogenomic databases
HOVERGEN	HBG006215.
KO	K00857.
Enzyme and pathway databases
SABIO-RK	P27158.
Gene expression databases
Genevestigator	P27158.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PROSITE	PS00603. TK_CELLULAR_TYPE. Partial match. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P27158.
ChEMBL	CHEMBL4022.
NextBio	604568.

Entry information

Entry name

KITH_RAT

Accession

Primary (citable) accession number: P27158
Secondary accession number(s): Q6LAG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program