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Protein

Thymidine kinase, cytosolic

Gene

Tk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Proton acceptorSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33ATPCurated8
Nucleotide bindingi58 – 60ATPBy similarity3
Nucleotide bindingi97 – 100ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • thymidine kinase activity Source: GO_Central
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • digestive tract development Source: RGD
  • DNA biosynthetic process Source: UniProtKB-KW
  • DNA metabolic process Source: GO_Central
  • fetal process involved in parturition Source: RGD
  • liver development Source: RGD
  • response to copper ion Source: RGD
  • response to cortisol Source: RGD
  • response to nutrient levels Source: RGD
  • response to toxic substance Source: RGD
  • skeletal muscle cell proliferation Source: RGD
  • thymidine metabolic process Source: RGD

Keywordsi

Molecular functionKinase, Transferase
Biological processDNA synthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.21. 5301.
SABIO-RKiP27158.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:Tk1
Synonyms:Tk-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621014. Tk1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4022.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001749502 – ›121Thymidine kinase, cytosolicAdd BLAST›120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP27158.
PRIDEiP27158.

Expressioni

Gene expression databases

BgeeiENSRNOG00000047314.

Interactioni

Subunit structurei

Homotetramer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8306437,EBI-8306437

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

MINTiMINT-8411768.
STRINGi10116.ENSRNOP00000067459.

Chemistry databases

BindingDBiP27158.

Structurei

3D structure databases

ProteinModelPortaliP27158.
SMRiP27158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiKOG3125. Eukaryota.
COG1435. LUCA.
HOVERGENiHBG006215.
InParanoidiP27158.
KOiK00857.
PhylomeDBiP27158.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiView protein in Pfam
PF00265. TK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYINLPTVL PISPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC
60 70 80 90 100
LVIKYAKDTR YSNSFSTHDR NTMDALPACM LKDVAQEALG VAVIGIDEGQ
110 120
FFPDIVDFCE TMANTGKTVI V
Length:121
Mass (Da):13,511
Last modified:August 1, 1992 - v1
Checksum:i8330BDFD9CDA9EB0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1211

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22642 mRNA. Translation: AAA75560.1.
Y17296 Genomic DNA. Translation: CAA76733.1.
X54173 Genomic DNA. Translation: CAA38106.1.
PIRiA39968.
RefSeqiNP_434687.1. NM_052800.1.
UniGeneiRn.147549.
Rn.195325.
Rn.233805.

Genome annotation databases

GeneIDi24834.
KEGGirno:24834.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiKITH_RAT
AccessioniPrimary (citable) accession number: P27158
Secondary accession number(s): Q6LAG4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 10, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families