Reviewed, UniProtKB/Swiss-Prot P27137 (TFDF1_RALEJ)

Names and origin

Protein names

Recommended name:
    Maleylacetate reductase 1
    EC=1.3.1.32
Alternative name(s):
    Maleylacetate reductase I

Gene names

Name:	tfdFI
Synonyms:	tfdF
Ordered Locus Names:	Reut_D6463

Encoded on

Plasmid pJP4 Ref.1 Ref.2
Plasmid pReut1 Ref.3

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-oxoadipate + NAD(P)+ = 2-maleylacetate + NAD(P)H.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	maleylacetate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Maleylacetate reductase 1		PRO_0000087851

Sequences

	Sequence		Length	Mass (Da)
	P27137-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 7D88C001B5B13376	FASTA	354	37,900
	10 20 30 40 50 60 MKKFTLDYLS PRVVFGAGTA SALPDEIGRL GARRPLVLSS PEQRELAKDI VRPIGDRVAG 70 80 90 100 110 120 YFDGATMHVP VDVIQKAERA FNDTDADSII AIGGGSTTGL AKILSMNLDV PSLVIPTTYA 130 140 150 160 170 180 GSEMTTIWGV TEGGMKRTGR DPKVLPKTVI YDPLLTVDLP LAISVTSALN AIAHAAEGLY 190 200 210 220 230 240 SADLNPVLET MCKQGICALF DAIPRLVAKP TDAEARTDAL FGAWMCGTAL CHLGMGLHHK 250 260 270 280 290 300 LCHTLGGTLN LPHAETHAIV LPHALAYNLP YAAPAERLLQ EVAGSSDVPS ALYDLARNAG 310 320 330 340 350 APLSLAEIGM RPEDIPRVRD LALRDQYPNP RPLESDALET LLVNAFRGRR PDFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4." Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F. J. Bacteriol. 172:2351-2359(1990) [PubMed: 2185214] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways." Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B. Environ. Microbiol. 6:655-668(2004) [PubMed: 15186344] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of plasmid 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35097 Genomic DNA. Translation: AAA98265.1. AY365053 Genomic DNA. Translation: AAR31036.1. CP000093 Genomic DNA. Translation: AAZ65761.1.
PIR	D35255.
RefSeq	YP_025384.1. YP_293618.1.
3D structure databases
SMR	P27137. Positions 3-348.
ModBase	Search...
Genome annotation databases
GeneID	2847475. 3607954.
GenomeReviews	Gene locus Reut_D6463 in contig CP000093_GR.
KEGG	reu:Reut_D6463.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742415.
OMA	WHEPEAD.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14414. REUT264198:REUT_D6463-MONOMER.
Family and domain databases
InterPro	IPR001670. ADH_Fe. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TFDF1_RALEJ

Accession

Primary (citable) accession number: P27137
Secondary accession number(s): Q46M69

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)