Reviewed, UniProtKB/Swiss-Prot P27136 (TFDE1_RALEJ)

Names and origin

Protein names

Recommended name:
    Carboxymethylenebutenolidase 1
    EC=3.1.1.45
Alternative name(s):
    Carboxymethylenebutenolidase I
    Dienelactone hydrolase I
      Short name=DLH I

Gene names

Name:	tfdEI
Synonyms:	tfdE
Ordered Locus Names:	Reut_D6464

Encoded on

Plasmid pJP4 Ref.1 Ref.2
Plasmid pReut1 Ref.3

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Ring cleavage of cyclic ester dienelactone to produce maleylacetate.
Catalytic activity	4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Subunit structure	Monomer.
Miscellaneous	Carboxymethylenebutenolidase is specific for dienelactone and has no activity toward enol-lactones.
Sequence similarities	Belongs to the dienelactone hydrolase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carboxymethylenebutenolidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	Carboxymethylenebutenolidase 1		PRO_0000161574
Sites
	Active site	123	1	By similarity
	Active site	171	1	By similarity
	Active site	201	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27136-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 1D0658C527CA87BA	FASTA	234	25,428
	10 20 30 40 50 60 MLSDGVEITS RSGGRFGAYL GKPTTDSAPI VVIAQEIFGI TPFIRETVEW LVGAGFGCVC 70 80 90 100 110 120 PDLYWRQAPN IELDANVPSE REQALALFRD FDMEAGVNDL SCAIEYARAL PFSNGRVAVV 130 140 150 160 170 180 GYCLGGALAF DVAARSLADC SIGYYGVGLE KKVSLVPAIT RPAMFHMGTK DHYVTEEARS 190 200 210 220 230 ILEEHFGRNK NLSLHWYPVG HSFARSSSPN FDQAATTVAN ARTLELLAML KDPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4." Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F. J. Bacteriol. 172:2351-2359(1990) [PubMed: 2185214] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways." Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B. Environ. Microbiol. 6:655-668(2004) [PubMed: 15186344] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of plasmid 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M35097 Genomic DNA. Translation: AAA98264.1. AY365053 Genomic DNA. Translation: AAR31037.1. CP000093 Genomic DNA. Translation: AAZ65762.1.
PIR	C35255.
RefSeq	YP_025385.1. YP_293619.1.
3D structure databases
HSSP	HSSP built from PDB template 1DIN based on UniProtKB P11453.
ModBase	Search...
Genome annotation databases
GeneID	2847423. 3607310.
GenomeReviews	Gene locus Reut_D6464 in contig CP000093_GR.
KEGG	reu:Reut_D6464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P27136.
OMA	PGIDITD.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_D6464-MON.
Family and domain databases
InterPro	IPR002925. Dienelactn_hydro. [Graphical view]
Pfam	PF01738. DLH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TFDE1_RALEJ

Accession

Primary (citable) accession number: P27136
Secondary accession number(s): Q46M68

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)