Reviewed, UniProtKB/Swiss-Prot P27129 (RFAJ_ECOLI)

Names and origin

Protein names

Recommended name:
    Lipopolysaccharide 1,2-glucosyltransferase
    EC=2.4.1.58

Gene names

Name:	rfaJ
Synonyms:	waaJ
Ordered Locus Names:	b3626, JW3601

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	UDP-glucose + lipopolysaccharide = UDP + D-glucosyl-lipopolysaccharide.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 8 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lipopolysaccharide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: InterPro lipopolysaccharide glucosyltransferase I activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
lysR	P03030	1	EBI-548107,EBI-561339

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Lipopolysaccharide 1,2-glucosyltransferase		PRO_0000206066
Experimental info
	Sequence conflict	310 – 338	29	RYKHL…KYYRK → DINIF in AAA24087. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27129-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 819428EA13F1959A	FASTA	338	39,040
	10 20 30 40 50 60 MDSFPAIEID KVKAWDFRLA NINTSECLNV AYGVDANYLD GVGVSITSIV LNNRHINLDF 70 80 90 100 110 120 YIIADVYNDG FFQKIAKLAE QNQLRITLYR INTDKLQCLP CTQVWSRAMY FRLFAFQLLG 130 140 150 160 170 180 LTLDRLLYLD ADVVCKGDIS QLLHLGLNGA VAAVVKDVEP MQEKAVSRLS DPELLGQYFN 190 200 210 220 230 240 SGVVYLDLKK WADAKLTEKA LSILMSKDNV YKYPDQDVMN VLLKGMTLFL PREYNTIYTI 250 260 270 280 290 300 KSELKDKTHQ NYKKLITEST LLIHYTGATK PWHKWAIYPS VKYYKIALEN SPWKDDSPRD 310 320 330 AKSIIEFKKR YKHLLVQHHY ISGIIAGVCY LCRKYYRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." Pradel E., Parker C.T., Schnaitman C.A. J. Bacteriol. 174:4736-4745(1992) [PubMed: 1624461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M80599 Genomic DNA. Translation: AAA24087.1. U00039 Genomic DNA. Translation: AAB18603.1. U00096 Genomic DNA. Translation: AAC76650.1. AP009048 Genomic DNA. Translation: BAE77666.1.
PIR	S47847.
RefSeq	AP_004165.1. NP_418083.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:10671N.
IntAct	P27129. 6 interactions.
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Genome annotation databases
GeneID	948142.
GenomeReviews	Gene locus JW3601 in contig AP009048_GR. Gene locus b3626 in contig U00096_GR.
KEGG	ecj:JW3601. eco:b3626.
Organism-specific databases
EchoBASE	EB1328.
EcoGene	EG11353. rfaJ.
CMR	Search...
Phylogenomic databases
HOGENOM	P27129.
OMA	P27129. MNILVTL.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11353-MON. MetaCyc:EG11353-MON.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. IPR013645. Glyco_transf_8N. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. PF08437. Glyco_transf_8N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFAJ_ECOLI

Accession

Primary (citable) accession number: P27129
Secondary accession number(s): Q2M7U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)