Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipopolysaccharide 1,2-glucosyltransferase

Gene

rfaJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Adds the glucose(II) group on the galactose(I) group of LPS.By similarity

Catalytic activityi

UDP-glucose + [lipopolysaccharide] = UDP + D-glucosyl-[lipopolysaccharide].

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11353-MONOMER
MetaCyc:EG11353-MONOMER
UniPathwayiUPA00958

Protein family/group databases

CAZyiGT8 Glycosyltransferase Family 8

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide 1,2-glucosyltransferase (EC:2.4.1.58)
Gene namesi
Name:rfaJ
Synonyms:waaJ
Ordered Locus Names:b3626, JW3601
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11353 rfaJ

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002060661 – 338Lipopolysaccharide 1,2-glucosyltransferaseAdd BLAST338

Proteomic databases

PaxDbiP27129
PRIDEiP27129

Interactioni

Protein-protein interaction databases

BioGridi4261893, 196 interactors
DIPiDIP-10671N
IntActiP27129, 6 interactors
STRINGi316385.ECDH10B_3808

Structurei

3D structure databases

ProteinModelPortaliP27129
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 8 family.Curated

Phylogenomic databases

eggNOGiENOG4105X1N Bacteria
COG1442 LUCA
HOGENOMiHOG000126190
InParanoidiP27129
KOiK03276
OMAiYISLATY
PhylomeDBiP27129

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR002495 Glyco_trans_8
IPR013645 Glyco_transf_8N
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01501 Glyco_transf_8, 1 hit
PF08437 Glyco_transf_8C, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

P27129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSFPAIEID KVKAWDFRLA NINTSECLNV AYGVDANYLD GVGVSITSIV
60 70 80 90 100
LNNRHINLDF YIIADVYNDG FFQKIAKLAE QNQLRITLYR INTDKLQCLP
110 120 130 140 150
CTQVWSRAMY FRLFAFQLLG LTLDRLLYLD ADVVCKGDIS QLLHLGLNGA
160 170 180 190 200
VAAVVKDVEP MQEKAVSRLS DPELLGQYFN SGVVYLDLKK WADAKLTEKA
210 220 230 240 250
LSILMSKDNV YKYPDQDVMN VLLKGMTLFL PREYNTIYTI KSELKDKTHQ
260 270 280 290 300
NYKKLITEST LLIHYTGATK PWHKWAIYPS VKYYKIALEN SPWKDDSPRD
310 320 330
AKSIIEFKKR YKHLLVQHHY ISGIIAGVCY LCRKYYRK
Length:338
Mass (Da):39,040
Last modified:October 1, 1994 - v2
Checksum:i819428EA13F1959A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti310 – 338RYKHL…KYYRK → DINIF in AAA24087 (PubMed:1624461).CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA Translation: AAA24087.1
U00039 Genomic DNA Translation: AAB18603.1
U00096 Genomic DNA Translation: AAC76650.1
AP009048 Genomic DNA Translation: BAE77666.1
PIRiS47847
RefSeqiNP_418083.1, NC_000913.3
WP_000376841.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76650; AAC76650; b3626
BAE77666; BAE77666; BAE77666
GeneIDi948142
KEGGiecj:JW3601
eco:b3626
PATRICifig|1411691.4.peg.3080

Entry informationi

Entry nameiRFAJ_ECOLI
AccessioniPrimary (citable) accession number: P27129
Secondary accession number(s): Q2M7U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health