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P27128 (RFAI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipopolysaccharide 1,3-galactosyltransferase

EC=2.4.1.44
Alternative name(s):
Lipopolysaccharide 3-alpha-galactosyltransferase
Gene names
Name:rfaI
Synonyms:waaI
Ordered Locus Names:b3627, JW3602
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

UDP-alpha-D-galactose + lipopolysaccharide = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose].

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Sequence similarities

Belongs to the glycosyltransferase 8 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Lipopolysaccharide 1,3-galactosyltransferase
PRO_0000206064

Sequences

Sequence LengthMass (Da)Tools
P27128 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 068CA689C1E0C1E0

FASTA33939,423
        10         20         30         40         50         60 
MQQVFFQETE FLNSVIDYDH KVETENLCLD IAYGTDKNFL FGCGISIASI LKYNEGSRLC 

        70         80         90        100        110        120 
FHIFTDYFGD DDRKYFDALA LQYKTRIKIY LINGDRLRSL PSTKNWTHAI YFRFVIADYF 

       130        140        150        160        170        180 
INKAPKVLYL DADIICQGTI EPLINFSFPD DKVAMVVTEG QADWWEKRAH SLGVAGIAKG 

       190        200        210        220        230        240 
YFNSGFLLIN TAQWAAQQVS ARAIAMLNEP EIIKKITHPD QDVLNMLLAD KLIFADIKYN 

       250        260        270        280        290        300 
TQFSLNYQLK ESFINPVTND TIFIHYIGPT KPWHDWAWDY PVSQAFMEAK NASPWKNTAL 

       310        320        330 
LKPNNSNQLR YSAKHMLKKH RYLKGFSNYL FYFIEKIKH 

« Hide

References

« Hide 'large scale' references
[1]"Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core."
Pradel E., Parker C.T., Schnaitman C.A.
J. Bacteriol. 174:4736-4745(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80599 Genomic DNA. Translation: AAA24086.1.
U00039 Genomic DNA. Translation: AAB18604.1.
U00096 Genomic DNA. Translation: AAC76651.1.
AP009048 Genomic DNA. Translation: BAE77665.1.
PIRC42982.
RefSeqNP_418084.1. NC_000913.2.
YP_491806.1. NC_007779.1.

3D structure databases

ProteinModelPortalP27128.
SMRP27128. Positions 29-274.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10670N.
IntActP27128. 3 interactions.
MINTMINT-1255919.
STRING511145.b3627.

Protein family/group databases

CAZyGT8. Glycosyltransferase Family 8.

Proteomic databases

PRIDEP27128.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76651; AAC76651; b3627.
BAE77665; BAE77665; BAE77665.
GeneID12934418.
948143.
KEGGecj:Y75_p3547.
eco:b3627.
PATRIC32122741. VBIEscCol129921_3747.

Organism-specific databases

EchoBASEEB1327.
EcoGeneEG11352. rfaI.

Phylogenomic databases

eggNOGCOG1442.
HOGENOMHOG000126190.
KOK03275.
OMAQHSDANK.
ProtClustDBPRK15171.

Enzyme and pathway databases

BioCycEcoCyc:EG11352-MONOMER.
ECOL316407:JW3602-MONOMER.
MetaCyc:EG11352-MONOMER.
UniPathwayUPA00958.

Gene expression databases

GenevestigatorP27128.

Family and domain databases

InterProIPR002495. Glyco_trans_8.
IPR013645. Glyco_transf_8N.
[Graphical view]
PfamPF01501. Glyco_transf_8. 1 hit.
PF08437. Glyco_transf_8C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFAI_ECOLI
AccessionPrimary (citable) accession number: P27128
Secondary accession number(s): Q2M7U1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families