P27128 (RFAI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipopolysaccharide 1,3-galactosyltransferase
EC=2.4.1.44

Alternative name(s):
Lipopolysaccharide 3-alpha-galactosyltransferase

Gene names

Name:	rfaI
Synonyms:	waaI
Ordered Locus Names:	b3627, JW3602

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	UDP-alpha-D-galactose + lipopolysaccharide = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose].
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 8 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	lipopolysaccharide 3-alpha-galactosyltransferase activity Inferred from mutant phenotype PubMed 9765561. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Lipopolysaccharide 1,3-galactosyltransferase		PRO_0000206064

Sequences

	Sequence		Length	Mass (Da)
	P27128 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 068CA689C1E0C1E0	FASTA	339	39,423
	10 20 30 40 50 60 MQQVFFQETE FLNSVIDYDH KVETENLCLD IAYGTDKNFL FGCGISIASI LKYNEGSRLC 70 80 90 100 110 120 FHIFTDYFGD DDRKYFDALA LQYKTRIKIY LINGDRLRSL PSTKNWTHAI YFRFVIADYF 130 140 150 160 170 180 INKAPKVLYL DADIICQGTI EPLINFSFPD DKVAMVVTEG QADWWEKRAH SLGVAGIAKG 190 200 210 220 230 240 YFNSGFLLIN TAQWAAQQVS ARAIAMLNEP EIIKKITHPD QDVLNMLLAD KLIFADIKYN 250 260 270 280 290 300 TQFSLNYQLK ESFINPVTND TIFIHYIGPT KPWHDWAWDY PVSQAFMEAK NASPWKNTAL 310 320 330 LKPNNSNQLR YSAKHMLKKH RYLKGFSNYL FYFIEKIKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." Pradel E., Parker C.T., Schnaitman C.A. J. Bacteriol. 174:4736-4745(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80599 Genomic DNA. Translation: AAA24086.1. U00039 Genomic DNA. Translation: AAB18604.1. U00096 Genomic DNA. Translation: AAC76651.1. AP009048 Genomic DNA. Translation: BAE77665.1.
PIR	C42982.
RefSeq	NP_418084.1. NC_000913.2. YP_491806.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P27128.
SMR	P27128. Positions 29-274.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10670N.
IntAct	P27128. 3 interactions.
MINT	MINT-1255919.
STRING	511145.b3627.
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Proteomic databases
PRIDE	P27128.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76651; AAC76651; b3627. BAE77665; BAE77665; BAE77665.
GeneID	12934418. 948143.
KEGG	ecj:Y75_p3547. eco:b3627.
PATRIC	32122741. VBIEscCol129921_3747.
Organism-specific databases
EchoBASE	EB1327.
EcoGene	EG11352. rfaI.
Phylogenomic databases
eggNOG	COG1442.
HOGENOM	HOG000126190.
KO	K03275.
OMA	QHSDANK.
ProtClustDB	PRK15171.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11352-MONOMER. ECOL316407:JW3602-MONOMER. MetaCyc:EG11352-MONOMER.
UniPathway	UPA00958.
Gene expression databases
Genevestigator	P27128.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. IPR013645. Glyco_transf_8N. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. PF08437. Glyco_transf_8C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFAI_ECOLI

Accession

Primary (citable) accession number: P27128
Secondary accession number(s): Q2M7U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program