Reviewed, UniProtKB/Swiss-Prot P27128 (RFAI_ECOLI)

Names and originHide

Protein names

Recommended name:
Lipopolysaccharide 1,3-galactosyltransferase
EC=2.4.1.44

Alternative name(s):
Lipopolysaccharide 3-alpha-galactosyltransferase

Gene names

Name:	rfaI
Synonyms:	waaI
Ordered Locus Names:	b3627, JW3602

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributesHide

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Catalytic activity	UDP-galactose + lipopolysaccharide = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose].
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 8 family.

OntologiesHide

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lipopolysaccharide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Lipopolysaccharide 1,3-galactosyltransferase		PRO_0000206064

SequencesHide

	Sequence		Length	Mass (Da)
	P27128-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 068CA689C1E0C1E0	FASTA	339	39,423
	10 20 30 40 50 60 MQQVFFQETE FLNSVIDYDH KVETENLCLD IAYGTDKNFL FGCGISIASI LKYNEGSRLC 70 80 90 100 110 120 FHIFTDYFGD DDRKYFDALA LQYKTRIKIY LINGDRLRSL PSTKNWTHAI YFRFVIADYF 130 140 150 160 170 180 INKAPKVLYL DADIICQGTI EPLINFSFPD DKVAMVVTEG QADWWEKRAH SLGVAGIAKG 190 200 210 220 230 240 YFNSGFLLIN TAQWAAQQVS ARAIAMLNEP EIIKKITHPD QDVLNMLLAD KLIFADIKYN 250 260 270 280 290 300 TQFSLNYQLK ESFINPVTND TIFIHYIGPT KPWHDWAWDY PVSQAFMEAK NASPWKNTAL 310 320 330 LKPNNSNQLR YSAKHMLKKH RYLKGFSNYL FYFIEKIKH

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." Pradel E., Parker C.T., Schnaitman C.A. J. Bacteriol. 174:4736-4745(1992) [PubMed: 1624461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	M80599 Genomic DNA. Translation: AAA24086.1. U00039 Genomic DNA. Translation: AAB18604.1. U00096 Genomic DNA. Translation: AAC76651.1. AP009048 Genomic DNA. Translation: BAE77665.1.
PIR	C42982.
RefSeq	AP_004164.1. NP_418084.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10670N.
MINT	MINT-1255919.
STRING	P27128.
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Genome annotation databases
EnsemblBacteria	EBESCT00000003850; EBESCP00000003850; EBESCG00000003146. EBESCT00000003851; EBESCP00000003851; EBESCG00000003146. EBESCT00000015353; EBESCP00000014644; EBESCG00000014413.
GeneID	948143.
GenomeReviews	Gene locus JW3602 in contig AP009048_GR. Gene locus b3627 in contig U00096_GR.
KEGG	ecj:JW3602. eco:b3627.
Organism-specific databases
EchoBASE	EB1327.
EcoGene	EG11352. rfaI.
CMR	Search...
Phylogenomic databases
eggNOG	COG1442.
HOGENOM	HBG417202.
OMA	MLADKAV.
ProtClustDB	CLSK880743.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11352-MONOMER. ECOL168927:B3627-MONOMER. MetaCyc:EG11352-MONOMER.
Gene expression databases
Genevestigator	P27128.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. IPR013645. Glyco_transf_8N. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. PF08437. Glyco_transf_8N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

RFAI_ECOLI

Accession

Primary (citable) accession number: P27128
Secondary accession number(s): Q2M7U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	July 13, 2010
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)