Reviewed, UniProtKB/Swiss-Prot P27128 (RFAI_ECOLI)

Names and origin

Protein names

Recommended name:
    Lipopolysaccharide 1,3-galactosyltransferase
    EC=2.4.1.44
Alternative name(s):
    Lipopolysaccharide 3-alpha-galactosyltransferase

Gene names

Name:	rfaI
Synonyms:	waaI
Ordered Locus Names:	b3627, JW3602

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	UDP-galactose + lipopolysaccharide = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose].
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 8 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lipopolysaccharide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Lipopolysaccharide 1,3-galactosyltransferase		PRO_0000206064

Sequences

	Sequence		Length	Mass (Da)
	P27128-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 068CA689C1E0C1E0	FASTA	339	39,423
	10 20 30 40 50 60 MQQVFFQETE FLNSVIDYDH KVETENLCLD IAYGTDKNFL FGCGISIASI LKYNEGSRLC 70 80 90 100 110 120 FHIFTDYFGD DDRKYFDALA LQYKTRIKIY LINGDRLRSL PSTKNWTHAI YFRFVIADYF 130 140 150 160 170 180 INKAPKVLYL DADIICQGTI EPLINFSFPD DKVAMVVTEG QADWWEKRAH SLGVAGIAKG 190 200 210 220 230 240 YFNSGFLLIN TAQWAAQQVS ARAIAMLNEP EIIKKITHPD QDVLNMLLAD KLIFADIKYN 250 260 270 280 290 300 TQFSLNYQLK ESFINPVTND TIFIHYIGPT KPWHDWAWDY PVSQAFMEAK NASPWKNTAL 310 320 330 LKPNNSNQLR YSAKHMLKKH RYLKGFSNYL FYFIEKIKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." Pradel E., Parker C.T., Schnaitman C.A. J. Bacteriol. 174:4736-4745(1992) [PubMed: 1624461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M80599 Genomic DNA. Translation: AAA24086.1. U00039 Genomic DNA. Translation: AAB18604.1. U00096 Genomic DNA. Translation: AAC76651.1. AP009048 Genomic DNA. Translation: BAE77665.1.
PIR	C42982.
RefSeq	AP_004164.1. NP_418084.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:10670N.
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Genome annotation databases
GeneID	948143.
GenomeReviews	Gene locus JW3602 in contig AP009048_GR. Gene locus b3627 in contig U00096_GR.
KEGG	ecj:JW3602. eco:b3627.
Organism-specific databases
EchoBASE	EB1327.
EcoGene	EG11352. rfaI.
CMR	Search...
Phylogenomic databases
HOGENOM	P27128.
OMA	P27128. MLADKAV.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11352-MON. MetaCyc:EG11352-MON.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. IPR013645. Glyco_transf_8N. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. PF08437. Glyco_transf_8N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFAI_ECOLI

Accession

Primary (citable) accession number: P27128
Secondary accession number(s): Q2M7U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)