Reviewed, UniProtKB/Swiss-Prot P27127 (RFAB_ECOLI)

Names and origin

Protein names

Recommended name:
    Lipopolysaccharide 1,6-galactosyltransferase
    EC=2.4.1.-
Alternative name(s):
    UDP-D-galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D-galactosyltransferase

Gene names

Name:	rfaB
Synonyms:	waaB
Ordered Locus Names:	b3628, JW3603

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adds a galactose goup to a glucose group of LPS.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 1 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rplB	P60422	1	EBI-1133312,EBI-543515

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Lipopolysaccharide 1,6-galactosyltransferase		PRO_0000080303

Sequences

	Sequence		Length	Mass (Da)
	P27127-1 [UniParc]. Last modified November 24, 2009. Version 2. Checksum: 191088896F4A45F4	FASTA	359	40,826
	10 20 30 40 50 60 MKIAFIGEAV SGFGGMETVI RDVITTFRQQ HIQSEMFFFC RNDKMDKGWL EGIKYSCSFS 70 80 90 100 110 120 NIRLGFLRRA KHIHALSKWL QEYQPDIVIC IDVISCLFAA KARKKSGIDM PVFSWPHFSL 130 140 150 160 170 180 DHKKHAEYIT CADYHLAISS GIKQQMINRG VAESTINVIF NPVETKDSVI PAPEEGETAT 190 200 210 220 230 240 FIYVGRMKFE GQKRVKDLLD GLSQAKGNWK LHVLGDGSDF EKCQAYGREL NIDDRIVWYG 250 260 270 280 290 300 WQQYPWELVQ QDIEKVSALL LTSSFEGFPM TLLEALSWGI PCISADCVSG PADIIQPDVN 310 320 330 340 350 GHLYQPGDIA GFVTLLNKYI AGEIHIEHEK IPASIDEFYQ SKYYDRLHKV IISAISRRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." Pradel E., Parker C.T., Schnaitman C.A. J. Bacteriol. 174:4736-4745(1992) [PubMed: 1624461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M80599 Genomic DNA. Translation: AAA24085.1. Different initiation. U00039 Genomic DNA. Translation: AAB18605.1. Different initiation. U00096 Genomic DNA. Translation: AAC76652.2. AP009048 Genomic DNA. Translation: BAE77664.1. Different initiation.
PIR	B42982.
RefSeq	AP_004163.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P27127. 1 interaction.
STRING	P27127.
Protein family/group databases
CAZy	GT4. Glycosyltransferase Family 4.
Genome annotation databases
GenomeReviews	Gene locus JW3603 in contig AP009048_GR. Gene locus b3628 in contig U00096_GR.
KEGG	ecj:JW3603. eco:b3628.
Organism-specific databases
EchoBASE	EB1326.
EcoGene	EG11351. rfaB.
CMR	Search...
Phylogenomic databases
HOGENOM	P27127.
OMA	DISVVYN
Enzyme and pathway databases
BioCyc	EcoCyc:EG11351-MON. ECOL168927:B3628-MON. MetaCyc:EG11351-MON.
Gene expression databases
Genevestigator	P27127.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFAB_ECOLI

Accession

Primary (citable) accession number: P27127
Secondary accession number(s): Q2M7U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 24, 2009
Last modified:	November 24, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)