P27111 (CYNR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator CynR

Alternative name(s):
Cyn operon transcriptional activator

Gene names

Name:	cynR
Ordered Locus Names:	b0338, JW5894

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR-cynTSX intergenic region. Ref.1
Subcellular location	Cytoplasm.
Induction	Negatively autoregulated. Ref.1
Sequence similarities	Contains 1 HTH lysR-type DNA-binding domain.
Sequence caution	The sequence AAB18062.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 299

299

HTH-type transcriptional regulator CynR

PRO_0000105612

Regions

Domain

1 – 58

58

HTH lysR-type

DNA binding

18 – 37

20

H-T-H motif By similarity

Secondary structure

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299

Helix Strand Turn

Details...

Beta strand

93 – 97

5

Helix

99 – 102

4

Turn

103 – 105

3

Helix

106 – 116

11

Beta strand

122 – 126

5

Helix

129 – 137

9

Beta strand

142 – 149

8

Beta strand

155 – 170

16

Helix

174 – 177

4

Beta strand

179 – 181

3

Helix

183 – 188

6

Beta strand

190 – 194

5

Helix

199 – 210

12

Beta strand

216 – 222

7

Helix

224 – 233

10

Beta strand

237 – 241

5

Helix

243 – 247

5

Beta strand

251 – 258

8

Beta strand

262 – 270

9

Helix

277 – 290

14

Sequences

	Sequence		Length	Mass (Da)
	P27111 [UniParc]. Last modified November 13, 2007. Version 5. Checksum: 5649F6FF2437624A	FASTA	299	32,961
	10 20 30 40 50 60 MLSRHINYFL AVAEHGSFTR AASALHVSQP ALSQQIRQLE ESLGVPLFDR SGRTIRLTDA 70 80 90 100 110 120 GEVWRQYASR ALQELGAGKR AIHDVADLTR GSLRIAVTPT FTSYFIGPLM ADFYARYPSI 130 140 150 160 170 180 TLQLQEMSQE KIEDMLCRDE LDVGIAFAPV HSPELEAIPL LTESLALVVA QHHPLAVHEQ 190 200 210 220 230 240 VALSRLHDEK LVLLSAEFAT REQIDHYCEK AGLHPQVVIE ANSISAVLEL IRRTSLSTLL 250 260 270 280 290 PAAIATQHDG LKAISLAPPL LERTAVLLRR KNSWQTAAAK AFLHMALDKC AVVGGNESR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Escherichia coli K-12 cyn operon is positively regulated by a member of the lysR family." Sung Y.-C., Fuchs J.A. J. Bacteriol. 174:3645-3650(1992) [PubMed: 1592818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS TRANSCRIPTIONAL REGULATOR, INDUCTION. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Expression and purification of the cynR regulatory gene product: CynR is a DNA-binding protein." Lamblin A.-F.J., Fuchs J.A. J. Bacteriol. 175:7990-7999(1993) [PubMed: 8253686] [Abstract] Cited for: IDENTIFICATION OF START CODON, DNA-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M93053 Genomic DNA. Translation: AAA23628.1.
U73857 Genomic DNA. Translation: AAB18062.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73441.3.
AP009048 Genomic DNA. Translation: BAE76120.1.

PIR

A41900.

RefSeq

NP_414872.3. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HXR	X-ray	2.05	A/B	63-299	[»]
3HFU	X-ray	2.60	A/B/C/D	63-299	[»]

ProteinModelPortal

P27111.

SMR

P27111. Positions 1-292.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9364N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004844; EBESCP00000004844; EBESCG00000003952.
EBESCT00000017065; EBESCP00000016356; EBESCG00000016124.

GeneID

945001.

GenomeReviews

Gene locus JW5894 in contig AP009048_GR.
Gene locus b0338 in contig U00096_GR.

KEGG

ecj:JW5894.
eco:b0338.

PATRIC

32115809. VBIEscCol129921_0346.

Organism-specific databases

EchoBASE

EB1391.

EcoGene

EG11421. cynR.

Phylogenomic databases

eggNOG

COG0583.

GeneTree

EBGT00050000008327.

HOGENOM

HBG685425.

OMA

IRCAREV.

PhylomeDB

P27111.

ProtClustDB

PRK11242.

Enzyme and pathway databases

BioCyc

EcoCyc:PD00291.

Gene expression databases

Genevestigator

P27111.

Family and domain databases

InterPro

IPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

KO

K11921.

Pfam

PF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]

PRINTS

PR00039. HTHLYSR.

PROSITE

PS50931. HTH_LYSR. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CYNR_ECOLI

Accession

Primary (citable) accession number: P27111
Secondary accession number(s): Q2MC86

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 100 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program