P27101 (TCBF_PSESQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Maleylacetate reductase
EC=1.3.1.32

Gene names

Name:

tcbF

Encoded on

Plasmid pP51

Organism

Pseudomonas sp. (strain P51)

Taxonomic identifier

65067 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-oxoadipate + NAD(P)+ = 2-maleylacetate + NAD(P)H.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.
Caution	Was originally (Ref.1) thought to be a trans-dienelactoneisomerase.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	2,4-dichlorophenoxyacetic acid catabolic process Non-traceable author statement Ref.1. Source: UniProtKB aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	maleylacetate reductase activity Non-traceable author statement Ref.1. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Maleylacetate reductase		PRO_0000087849

Sequences

	Sequence		Length	Mass (Da)
	P27101 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C72A9D2671FFFAB3	FASTA	352	37,499
	10 20 30 40 50 60 MNFIHDPLTP RVLFGAGRLQ SLGEELKLLG IRRVLVISTP EQRELANQVA ALIPGSVAGF 70 80 90 100 110 120 FDRATMHVPS QIVDQAASVA RELGVDSYVA PGGGSTIGLA KMLALHSSLP IVAIPTTYAG 130 140 150 160 170 180 SEMTSIYGVT ENELKKTGRD RRVLARTVIY DPELTFGLPT GISVTSGLNA IAHAVEGLYA 190 200 210 220 230 240 PEVNPILAIM AQQGIAALAK SIPTIRSAPT DLEARSQAQY GAWLCGSVLG NVSMALHHKL 250 260 270 280 290 300 CHTLGGTFNL PHAETHTVVL PHALAYNTPA IPRANAWLQE ALATREPAQA LFDLAKSNGA 310 320 330 340 350 PVSLQSIGMK EADLDRACEL VMSAQYPNPR PLEKHAIANL LRRAYLGEPP QP

References

[1]

"Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates." van der Meer J.R., Eggen R.I., Zehnder A.J., de Vos W.M. J. Bacteriol. 173:2425-2434(1991) [PubMed: 2013566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57629 Genomic DNA. Translation: AAD13629.1.
PIR	E43673.
3D structure databases
ProteinModelPortal	P27101.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14415.
Family and domain databases
InterPro	IPR001670. ADH_Fe. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCBF_PSESQ

Accession

Primary (citable) accession number: P27101

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	September 21, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program