P27100 (TCBE_PSESQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carboxymethylenebutenolidase
EC=3.1.1.45

Alternative name(s):
Dienelactone hydrolase
Short name=DLH

Gene names

Name:

tcbE

Encoded on

Plasmid pP51

Organism

Pseudomonas sp. (strain P51)

Taxonomic identifier

65067 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ring cleavage of cyclic ester dienelactone to produce maleylacetate.
Catalytic activity	4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Subunit structure	Monomer.
Miscellaneous	Carboxymethylenebutenolidase is specific for dienelactone and has no activity toward enol-lactones.
Sequence similarities	Belongs to the dienelactone hydrolase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Hydrolase Serine esterase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW carboxymethylenebutenolidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Carboxymethylenebutenolidase		PRO_0000161573
Sites
	Active site	123	1	By similarity
	Active site	171	1	By similarity
	Active site	201	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27100 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: DB57EAD5229C03C8	FASTA	238	25,812
	10 20 30 40 50 60 MLTEGLSIDA KGGGRFGAHL QLPARGRGPV VMVAQEIFGV NPFMTEVLAW LASEGFVGLC 70 80 90 100 110 120 PDLYWRHGPG IEFDPNDEVQ RARALGMFRD YKLEDGVADL RATVAYAASQ PFCDGGVAVI 130 140 150 160 170 180 GYCLGGALAY EVAAEGFAQC CVGYYGVGFE KRLERARLVK TPSMFHMGTN DHFVTAEARQ 190 200 210 220 230 LITNAFEANP AIALHWYDAG HSFARASSPN FSPEATRTAN ARTLEMLKRM KPIGTIGQ

References

[1]	"Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates." van der Meer J.R., Eggen R.I., Zehnder A.J., de Vos W.M. J. Bacteriol. 173:2425-2434(1991) [PubMed: 2013566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57629 Genomic DNA. Translation: AAD13628.1.
PIR	D43673.
3D structure databases
ProteinModelPortal	P27100.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14406.
Family and domain databases
InterPro	IPR002925. Dienelactn_hydro. [Graphical view]
Pfam	PF01738. DLH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCBE_PSESQ

Accession

Primary (citable) accession number: P27100

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 28, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program