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P27099 (TCBD_PSESQ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloromuconate cycloisomerase

EC=5.5.1.7
Alternative name(s):
Muconate cycloisomerase II
Gene names
Name:tcbD
Encoded onPlasmid pP51
OrganismPseudomonas sp. (strain P51)
Taxonomic identifier65067 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceae

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactor

Manganese. Ref.2

Pathway

Aromatic compound metabolism; 3-chlorocatechol degradation.

Miscellaneous

Chloromuconate cycloisomerase II is highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

Sequence similarities

Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Chloromuconate cycloisomerase
PRO_0000171254

Sites

Active site1651Proton acceptor
Active site3231Proton donor
Metal binding1941Manganese
Metal binding2201Manganese
Metal binding2451Manganese

Secondary structure

................................................................. 370
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P27099 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 8C6ADA522C63508A

FASTA37039,487
        10         20         30         40         50         60 
MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVKAGG LVGIGEGGSV GGPTWGSESA 

        70         80         90        100        110        120 
ETIKVIIDNY LAPLLVGKDA SNLSQARVLM DRAVTGNLSA KAAIDIALHD LKARALNLSI 

       130        140        150        160        170        180 
ADLIGGTMRT SIPIAWTLAS GDTARDIDSA LEMIETRRHN RFKVKLGART PAQDLEHIRS 

       190        200        210        220        230        240 
IVKAVGDRAS VRVDVNQGWD EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV 

       250        260        270        280        290        300 
AILADESLSS LSSAFELARD HAVDAFSLKL CNMGGIANTL KVAAVAEAAG ISSYGGTMLD 

       310        320        330        340        350        360 
STVGTAAALH VYATLPSLPY GCELIGPWVL GDRLTQQDLE IKDFEVHLPL GSGLGVDLDH 

       370 
DKVRHYTRAA 

« Hide

References

[1]"Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates."
van der Meer J.R., Eggen R.I., Zehnder A.J., de Vos W.M.
J. Bacteriol. 173:2425-2434(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The structure of Pseudomonas P51 Cl-muconate lactonizing enzyme: co-evolution of structure and dynamics with the dehalogenation function."
Kajander T., Lehtioe L., Schloemann M., Goldman A.
Protein Sci. 12:1855-1864(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), COFACTOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57629 Genomic DNA. Translation: AAD13626.1.
PIRB43673.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NU5X-ray1.95A1-370[»]
ProteinModelPortalP27099.
SMRP27099. Positions 1-369.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14405.
UniPathwayUPA00083.

Family and domain databases

InterProIPR013370. Chloromuconate_cycloisomerase.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]
PANTHERPTHR13794. PTHR13794. 1 hit.
PTHR13794:SF12. PTHR13794:SF12. 1 hit.
PfamPF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTSM00922. MR_MLE. 1 hit.
[Graphical view]
TIGRFAMsTIGR02534. mucon_cyclo. 1 hit.
PROSITEPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP27099.

Entry information

Entry nameTCBD_PSESQ
AccessionPrimary (citable) accession number: P27099
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways