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P27099

- TCBD_PSESQ

UniProt

P27099 - TCBD_PSESQ

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Protein

Chloromuconate cycloisomerase

Gene

tcbD

Organism
Pseudomonas sp. (strain P51)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactori

Manganese.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei165 – 1651Proton acceptor
Metal bindingi194 – 1941Manganese
Metal bindingi220 – 2201Manganese
Metal bindingi245 – 2451Manganese
Active sitei323 – 3231Proton donor

GO - Molecular functioni

  1. chloromuconate cycloisomerase activity Source: UniProtKB
  2. manganese ion binding Source: InterPro
  3. muconate cycloisomerase activity Source: InterPro

GO - Biological processi

  1. 2,4-dichlorophenoxyacetic acid catabolic process Source: UniProtKB
  2. cellular amino acid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14405.
UniPathwayiUPA00083.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloromuconate cycloisomerase (EC:5.5.1.7)
Alternative name(s):
Muconate cycloisomerase II
Gene namesi
Name:tcbD
Encoded oniPlasmid pP510 Publication
OrganismiPseudomonas sp. (strain P51)
Taxonomic identifieri65067 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceae

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Chloromuconate cycloisomerasePRO_0000171254Add
BLAST

Structurei

Secondary structure

1
370
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 2120
Beta strandi24 – 3815
Beta strandi41 – 477
Turni51 – 555
Helixi60 – 6910
Helixi71 – 755
Helixi83 – 9311
Helixi98 – 11518
Helixi120 – 1245
Beta strandi130 – 1345
Beta strandi136 – 1383
Helixi143 – 15513
Beta strandi160 – 1656
Beta strandi167 – 1693
Helixi171 – 18515
Helixi186 – 1883
Beta strandi190 – 1945
Helixi201 – 21414
Beta strandi218 – 2203
Helixi228 – 23710
Beta strandi239 – 2457
Helixi251 – 2599
Beta strandi264 – 2685
Helixi270 – 2734
Helixi276 – 28914
Beta strandi292 – 2954
Helixi302 – 31211
Helixi326 – 3294
Beta strandi330 – 3323
Beta strandi334 – 3374
Beta strandi345 – 3473
Beta strandi351 – 3533
Helixi360 – 3667

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NU5X-ray1.95A1-370[»]
ProteinModelPortaliP27099.
SMRiP27099. Positions 1-369.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27099.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27099-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVKAGG LVGIGEGGSV
60 70 80 90 100
GGPTWGSESA ETIKVIIDNY LAPLLVGKDA SNLSQARVLM DRAVTGNLSA
110 120 130 140 150
KAAIDIALHD LKARALNLSI ADLIGGTMRT SIPIAWTLAS GDTARDIDSA
160 170 180 190 200
LEMIETRRHN RFKVKLGART PAQDLEHIRS IVKAVGDRAS VRVDVNQGWD
210 220 230 240 250
EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV AILADESLSS
260 270 280 290 300
LSSAFELARD HAVDAFSLKL CNMGGIANTL KVAAVAEAAG ISSYGGTMLD
310 320 330 340 350
STVGTAAALH VYATLPSLPY GCELIGPWVL GDRLTQQDLE IKDFEVHLPL
360 370
GSGLGVDLDH DKVRHYTRAA
Length:370
Mass (Da):39,487
Last modified:August 1, 1992 - v1
Checksum:i8C6ADA522C63508A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57629 Genomic DNA. Translation: AAD13626.1.
PIRiB43673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57629 Genomic DNA. Translation: AAD13626.1 .
PIRi B43673.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NU5 X-ray 1.95 A 1-370 [» ]
ProteinModelPortali P27099.
SMRi P27099. Positions 1-369.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00083 .
BioCyci MetaCyc:MONOMER-14405.

Miscellaneous databases

EvolutionaryTracei P27099.

Family and domain databases

Gene3Di 3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProi IPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view ]
PANTHERi PTHR13794. PTHR13794. 1 hit.
Pfami PF02746. MR_MLE_N. 1 hit.
[Graphical view ]
SMARTi SM00922. MR_MLE. 1 hit.
[Graphical view ]
SUPFAMi SSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsi TIGR02534. mucon_cyclo. 1 hit.
PROSITEi PS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates."
    van der Meer J.R., Eggen R.I., Zehnder A.J., de Vos W.M.
    J. Bacteriol. 173:2425-2434(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The structure of Pseudomonas P51 Cl-muconate lactonizing enzyme: co-evolution of structure and dynamics with the dehalogenation function."
    Kajander T., Lehtioe L., Schloemann M., Goldman A.
    Protein Sci. 12:1855-1864(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), COFACTOR.

Entry informationi

Entry nameiTCBD_PSESQ
AccessioniPrimary (citable) accession number: P27099
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Chloromuconate cycloisomerase II is highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3