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Protein

Chloromuconate cycloisomerase

Gene

tcbD

Organism
Pseudomonas sp. (strain P51)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Chloromuconate cycloisomerase II is highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

Catalytic activityi

(2R)-2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactori

Mn2+1 Publication

Pathwayi: 3-chlorocatechol degradation

This protein is involved in the pathway 3-chlorocatechol degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-chlorocatechol degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei165Proton acceptor1
Metal bindingi194Manganese1
Metal bindingi220Manganese1
Metal bindingi245Manganese1
Active sitei323Proton donor1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAromatic hydrocarbons catabolism
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14405
UniPathwayiUPA00083

Names & Taxonomyi

Protein namesi
Recommended name:
Chloromuconate cycloisomerase (EC:5.5.1.7)
Alternative name(s):
Muconate cycloisomerase II
Gene namesi
Name:tcbD
Encoded oniPlasmid pP510 Publication
OrganismiPseudomonas sp. (strain P51)
Taxonomic identifieri65067 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceae

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712541 – 370Chloromuconate cycloisomeraseAdd BLAST370

Proteomic databases

PRIDEiP27099

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 21Combined sources20
Beta strandi24 – 38Combined sources15
Beta strandi41 – 47Combined sources7
Turni51 – 55Combined sources5
Helixi60 – 69Combined sources10
Helixi71 – 75Combined sources5
Helixi83 – 93Combined sources11
Helixi98 – 115Combined sources18
Helixi120 – 124Combined sources5
Beta strandi130 – 134Combined sources5
Beta strandi136 – 138Combined sources3
Helixi143 – 155Combined sources13
Beta strandi160 – 165Combined sources6
Beta strandi167 – 169Combined sources3
Helixi171 – 185Combined sources15
Helixi186 – 188Combined sources3
Beta strandi190 – 194Combined sources5
Helixi201 – 214Combined sources14
Beta strandi218 – 220Combined sources3
Helixi228 – 237Combined sources10
Beta strandi239 – 245Combined sources7
Helixi251 – 259Combined sources9
Beta strandi264 – 268Combined sources5
Helixi270 – 273Combined sources4
Helixi276 – 289Combined sources14
Beta strandi292 – 295Combined sources4
Helixi302 – 312Combined sources11
Helixi326 – 329Combined sources4
Beta strandi330 – 332Combined sources3
Beta strandi334 – 337Combined sources4
Beta strandi345 – 347Combined sources3
Beta strandi351 – 353Combined sources3
Helixi360 – 366Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NU5X-ray1.95A1-370[»]
ProteinModelPortaliP27099
SMRiP27099
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27099

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK01860

Family and domain databases

CDDicd03318 MLE, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 2 hits
InterProiView protein in InterPro
IPR013370 Chloromuconate_cycloisomerase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR029065 Enolase_C-like
IPR018110 Mandel_Rmase/mucon_lact_enz_CS
IPR013342 Mandelate_racemase_C
IPR013341 Mandelate_racemase_N_dom
PfamiView protein in Pfam
PF13378 MR_MLE_C, 1 hit
PF02746 MR_MLE_N, 1 hit
SFLDiSFLDG01258 (chloro)muconate_cycloisomeras, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM00922 MR_MLE, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR02534 mucon_cyclo, 1 hit
PROSITEiView protein in PROSITE
PS00908 MR_MLE_1, 1 hit
PS00909 MR_MLE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P27099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVKAGG LVGIGEGGSV
60 70 80 90 100
GGPTWGSESA ETIKVIIDNY LAPLLVGKDA SNLSQARVLM DRAVTGNLSA
110 120 130 140 150
KAAIDIALHD LKARALNLSI ADLIGGTMRT SIPIAWTLAS GDTARDIDSA
160 170 180 190 200
LEMIETRRHN RFKVKLGART PAQDLEHIRS IVKAVGDRAS VRVDVNQGWD
210 220 230 240 250
EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV AILADESLSS
260 270 280 290 300
LSSAFELARD HAVDAFSLKL CNMGGIANTL KVAAVAEAAG ISSYGGTMLD
310 320 330 340 350
STVGTAAALH VYATLPSLPY GCELIGPWVL GDRLTQQDLE IKDFEVHLPL
360 370
GSGLGVDLDH DKVRHYTRAA
Length:370
Mass (Da):39,487
Last modified:August 1, 1992 - v1
Checksum:i8C6ADA522C63508A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57629 Genomic DNA Translation: AAD13626.1
PIRiB43673

Genome annotation databases

KEGGiag:AAD13626

Similar proteinsi

Entry informationi

Entry nameiTCBD_PSESQ
AccessioniPrimary (citable) accession number: P27099
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

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