P27098 (TCBC_PSESQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chlorocatechol 1,2-dioxygenase
EC=1.13.11.-

Gene names

Name:

tcbC

Encoded on

Plasmid pP51

Organism

Pseudomonas sp. (strain P51)

Taxonomic identifier

65067 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Preferentially converts 3,4-dichlorocatechol as opposed to other chlorinated catechols. Retains diminished activity toward non-chlorinated substrates.
Catalytic activity	3,4-dichlorocatechol + O2 = 2,3-dichloro -muconate.
Cofactor	Binds 1 Fe3+ ion per subunit.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Sequence similarities	Belongs to the intradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW catechol-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	catechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro chlorocatechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro ferric iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Chlorocatechol 1,2-dioxygenase		PRO_0000085089
Sites
	Metal binding	130	1	Iron By similarity
	Metal binding	164	1	Iron By similarity
	Metal binding	188	1	Iron By similarity
	Metal binding	190	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27098 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 8867F76282D7C6D7	FASTA	251	27,554
	10 20 30 40 50 60 MNERVKQVAS ALVDAIQKTL TEQRVTEEEW RAGVGYMMKL AEAKEVAVLL DAFFNHTIVD 70 80 90 100 110 120 LKAQATRGSR PAMQGPYFLE GAPVVAGALK TYEDDSHHPL VIRGAVRTDD GAPAAGAVID 130 140 150 160 170 180 VWHSTPDGKY SGIHDQIPTD MYRGKVVADA QGKYAVRTTM PAPYQIPNKG PTGVLLEMMG 190 200 210 220 230 240 SHTWRPAHVH FKVRKDGFAP LTTQYYFEGG DWVDSDCCKG VAPDLVMPTK TEGGAQVMDI 250 DFVIERAREH V

References

[1]	"Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates." van der Meer J.R., Eggen R.I., Zehnder A.J., de Vos W.M. J. Bacteriol. 173:2425-2434(1991) [PubMed: 2013566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57629 Genomic DNA. Translation: AAD13625.1.
PIR	A43673.
3D structure databases
ProteinModelPortal	P27098.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14402.
Family and domain databases
InterPro	IPR007535. Catechol_dOase_N. IPR012817. Chlorcchol_dOase. IPR000627. Intradiol_dOase_C. IPR015889. Intradiol_dOase_core. [Graphical view]
Gene3D	G3DSA:2.60.130.10. Intradiol_dOase_core. 1 hit.
Pfam	PF00775. Dioxygenase_C. 1 hit. PF04444. Dioxygenase_N. 1 hit. [Graphical view]
SUPFAM	SSF49482. Dioxygenase. 1 hit.
TIGRFAMs	TIGR02465. Chlorocat_1_2. 1 hit.
PROSITE	PS00083. INTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCBC_PSESQ

Accession

Primary (citable) accession number: P27098

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program