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Protein

Protein 60A

Gene

gbb

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the growth of imaginal tissues and for patterning of the adult wing.1 Publication

GO - Molecular functioni

  • transforming growth factor beta receptor binding Source: FlyBase

GO - Biological processi

  • dorsal closure Source: FlyBase
  • female germ-line stem cell population maintenance Source: FlyBase
  • growth Source: InterPro
  • imaginal disc-derived wing vein morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein specification Source: FlyBase
  • neuromuscular junction development Source: FlyBase
  • neuromuscular synaptic transmission Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • positive regulation of muscle organ development Source: FlyBase
  • positive regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • regulation of neurotransmitter secretion Source: FlyBase
  • synaptic vesicle cycle Source: FlyBase
  • transforming growth factor beta receptor signaling pathway Source: FlyBase
  • ventral cord development Source: FlyBase
  • wing disc pattern formation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Developmental protein, Growth factor

Enzyme and pathway databases

ReactomeiR-DME-2129379. Molecules associated with elastic fibres.
SignaLinkiP27091.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 60A
Alternative name(s):
Protein glass bottom boat
Gene namesi
Name:gbb
Synonyms:60A, gbb-60A, TGFb-60A
ORF Names:CG5562
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0024234. gbb.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • dense core granule Source: FlyBase
  • endomembrane system Source: FlyBase
  • extracellular region Source: FlyBase
  • extracellular space Source: UniProtKB-KW
  • type III terminal bouton Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Flies exhibit transparent larvae, and a reduction of the adult wing size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3636Sequence analysisAdd
BLAST
Propeptidei37 – 335299Sequence analysisPRO_0000033660Add
BLAST
Chaini336 – 455120Protein 60APRO_0000033661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi238 – 2381N-linked (GlcNAc...)Sequence analysis
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence analysis
Disulfide bondi354 ↔ 420By similarity
Disulfide bondi383 ↔ 452By similarity
Disulfide bondi387 ↔ 454By similarity
Glycosylationi396 – 3961N-linked (GlcNAc...)Sequence analysis
Disulfide bondi419 – 419InterchainBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP27091.
PRIDEiP27091.

Expressioni

Tissue specificityi

Expressed in cells of the developing foregut and hindgut during germ band retraction and later embryonic stages. Expressed in the wing disk, mainly in the posterior compartment in the pteropleural and medial regions extending into the progenitors of the scutellum. High levels are found within the posterior and anterior compartments of the wing pouch and low levels in the hinge region. In the eye/antennal disk, expression is highest anterior to the morphogenetic furrow and in the medial regions with lower levels of expression posterior to the morphogenetic furrow. Expressed throughout the posterior compartment of the leg imaginal disks and within the ventral anterior compartment.2 Publications

Developmental stagei

Expressed throughout development with peaks of transcription during early embryogenesis, in pupae, and in adult males.2 Publications

Gene expression databases

BgeeiP27091.
GenevisibleiP27091. DM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

GO - Molecular functioni

  • transforming growth factor beta receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi63372. 10 interactions.
DIPiDIP-19466N.
IntActiP27091. 3 interactions.
MINTiMINT-978437.
STRINGi7227.FBpp0072036.

Structurei

3D structure databases

ProteinModelPortaliP27091.
SMRiP27091. Positions 353-455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000118883.
InParanoidiP27091.
KOiK16621.
OMAiALRMSNI.
OrthoDBiEOG7J9VPQ.
PhylomeDBiP27091.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRNTSEA VAVLASLGLG MVLLMFVATT PPAVEATQSG IYIDNGKDQT
60 70 80 90 100
IMHRVLSEDD KLDVSYEILE FLGIAERPTH LSSHQLSLRK SAPKFLLDVY
110 120 130 140 150
HRITAEEGLS DQDEDDDYER GHRSRRSADL EEDEGEQQKN FITDLDKRAI
160 170 180 190 200
DESDIIMTFL NKRHHNVDEL RHEHGRRLWF DVSNVPNDNY LVMAELRIYQ
210 220 230 240 250
NANEGKWLTA NREFTITVYA IGTGTLGQHT MEPLSSVNTT GDYVGWLELN
260 270 280 290 300
VTEGLHEWLV KSKDNHGIYI GAHAVNRPDR EVKLDDIGLI HRKVDDEFQP
310 320 330 340 350
FMIGFFRGPE LIKATAHSSH HRSKRSASHP RKRKKSVSPN NVPLLEPMES
360 370 380 390 400
TRSCQMQTLY IDFKDLGWHD WIIAPEGYGA FYCSGECNFP LNAHMNATNH
410 420 430 440 450
AIVQTLVHLL EPKKVPKPCC APTRLGALPV LYHLNDENVN LKKYRNMIVK

SCGCH
Length:455
Mass (Da):51,687
Last modified:August 1, 1992 - v1
Checksum:iC8FA795556341F94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77012 Genomic DNA. Translation: AAA28306.1.
M84795 mRNA. Translation: AAA28307.1.
AE013599 Genomic DNA. Translation: AAF47075.1.
PIRiA43918.
RefSeqiNP_001286786.1. NM_001299857.1.
NP_477340.1. NM_057992.3.
UniGeneiDm.4758.

Genome annotation databases

EnsemblMetazoaiFBtr0072127; FBpp0072036; FBgn0024234.
FBtr0343274; FBpp0309939; FBgn0024234.
GeneIDi37778.
KEGGidme:Dmel_CG5562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77012 Genomic DNA. Translation: AAA28306.1.
M84795 mRNA. Translation: AAA28307.1.
AE013599 Genomic DNA. Translation: AAF47075.1.
PIRiA43918.
RefSeqiNP_001286786.1. NM_001299857.1.
NP_477340.1. NM_057992.3.
UniGeneiDm.4758.

3D structure databases

ProteinModelPortaliP27091.
SMRiP27091. Positions 353-455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63372. 10 interactions.
DIPiDIP-19466N.
IntActiP27091. 3 interactions.
MINTiMINT-978437.
STRINGi7227.FBpp0072036.

Proteomic databases

PaxDbiP27091.
PRIDEiP27091.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072127; FBpp0072036; FBgn0024234.
FBtr0343274; FBpp0309939; FBgn0024234.
GeneIDi37778.
KEGGidme:Dmel_CG5562.

Organism-specific databases

CTDi37778.
FlyBaseiFBgn0024234. gbb.

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000118883.
InParanoidiP27091.
KOiK16621.
OMAiALRMSNI.
OrthoDBiEOG7J9VPQ.
PhylomeDBiP27091.

Enzyme and pathway databases

ReactomeiR-DME-2129379. Molecules associated with elastic fibres.
SignaLinkiP27091.

Miscellaneous databases

ChiTaRSiGbeta13F. fly.
GenomeRNAii37778.
NextBioi805357.
PROiP27091.

Gene expression databases

BgeeiP27091.
GenevisibleiP27091. DM.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila 60A gene, another transforming growth factor beta family member, is closely related to human bone morphogenetic proteins."
    Wharton K.A., Thomsen G.H., Gelbart W.M.
    Proc. Natl. Acad. Sci. U.S.A. 88:9214-9218(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
  2. "Sequence, biochemical characterization, and developmental expression of a new member of the TGF-beta superfamily in Drosophila melanogaster."
    Doctor J.S., Jackson P.D., Rashka K.E., Visalli M., Hoffmann F.M.
    Dev. Biol. 151:491-505(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. "TGF-beta/BMP superfamily members, Gbb-60A and Dpp, cooperate to provide pattern information and establish cell identity in the Drosophila wing."
    Khalsa O., Yoon J.-W., Torres-Schumann S., Wharton K.A.
    Development 125:2723-2734(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry namei60A_DROME
AccessioniPrimary (citable) accession number: P27091
Secondary accession number(s): Q9W1I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.