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Protein

Protein 60A

Gene

gbb

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the growth of imaginal tissues and for patterning of the adult wing.1 Publication

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • growth factor activity Source: UniProtKB-KW
  • transforming growth factor beta receptor binding Source: FlyBase

GO - Biological processi

  • BMP signaling pathway Source: GO_Central
  • dorsal closure Source: FlyBase
  • female germ-line stem cell population maintenance Source: FlyBase
  • imaginal disc-derived wing vein morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein specification Source: FlyBase
  • neuromuscular junction development Source: FlyBase
  • neuromuscular synaptic transmission Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • positive regulation of muscle organ development Source: FlyBase
  • positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
  • positive regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • regulation of apoptotic process Source: GO_Central
  • regulation of MAPK cascade Source: GO_Central
  • regulation of neurotransmitter secretion Source: FlyBase
  • SMAD protein signal transduction Source: GO_Central
  • synaptic vesicle cycle Source: FlyBase
  • ventral cord development Source: FlyBase
  • wing disc pattern formation Source: FlyBase

Keywordsi

Molecular functionCytokine, Developmental protein, Growth factor

Enzyme and pathway databases

ReactomeiR-DME-2129379. Molecules associated with elastic fibres.
SignaLinkiP27091.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 60A
Alternative name(s):
Protein glass bottom boat
Gene namesi
Name:gbb
Synonyms:60A, gbb-60A, TGFb-60A
ORF Names:CG5562
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0024234. gbb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Flies exhibit transparent larvae, and a reduction of the adult wing size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
PropeptideiPRO_000003366037 – 335Sequence analysisAdd BLAST299
ChainiPRO_0000033661336 – 455Protein 60AAdd BLAST120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi238N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi250N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi354 ↔ 420By similarity
Disulfide bondi383 ↔ 452By similarity
Disulfide bondi387 ↔ 454By similarity
Glycosylationi396N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi419InterchainBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP27091.
PRIDEiP27091.

Expressioni

Tissue specificityi

Expressed in cells of the developing foregut and hindgut during germ band retraction and later embryonic stages. Expressed in the wing disk, mainly in the posterior compartment in the pteropleural and medial regions extending into the progenitors of the scutellum. High levels are found within the posterior and anterior compartments of the wing pouch and low levels in the hinge region. In the eye/antennal disk, expression is highest anterior to the morphogenetic furrow and in the medial regions with lower levels of expression posterior to the morphogenetic furrow. Expressed throughout the posterior compartment of the leg imaginal disks and within the ventral anterior compartment.2 Publications

Developmental stagei

Expressed throughout development with peaks of transcription during early embryogenesis, in pupae, and in adult males.2 Publications

Gene expression databases

BgeeiFBgn0024234.
ExpressionAtlasiP27091. differential.
GenevisibleiP27091. DM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • growth factor activity Source: UniProtKB-KW
  • transforming growth factor beta receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi63372. 30 interactors.
DIPiDIP-19466N.
IntActiP27091. 3 interactors.
MINTiMINT-978437.
STRINGi7227.FBpp0072036.

Structurei

3D structure databases

ProteinModelPortaliP27091.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000118883.
InParanoidiP27091.
KOiK16621.
OMAiIYKDCVV.
OrthoDBiEOG091G0C2J.
PhylomeDBiP27091.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiView protein in InterPro
IPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_propeptide.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiView protein in Pfam
PF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
SMARTiView protein in SMART
SM00204. TGFB. 1 hit.
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiView protein in PROSITE
PS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRNTSEA VAVLASLGLG MVLLMFVATT PPAVEATQSG IYIDNGKDQT
60 70 80 90 100
IMHRVLSEDD KLDVSYEILE FLGIAERPTH LSSHQLSLRK SAPKFLLDVY
110 120 130 140 150
HRITAEEGLS DQDEDDDYER GHRSRRSADL EEDEGEQQKN FITDLDKRAI
160 170 180 190 200
DESDIIMTFL NKRHHNVDEL RHEHGRRLWF DVSNVPNDNY LVMAELRIYQ
210 220 230 240 250
NANEGKWLTA NREFTITVYA IGTGTLGQHT MEPLSSVNTT GDYVGWLELN
260 270 280 290 300
VTEGLHEWLV KSKDNHGIYI GAHAVNRPDR EVKLDDIGLI HRKVDDEFQP
310 320 330 340 350
FMIGFFRGPE LIKATAHSSH HRSKRSASHP RKRKKSVSPN NVPLLEPMES
360 370 380 390 400
TRSCQMQTLY IDFKDLGWHD WIIAPEGYGA FYCSGECNFP LNAHMNATNH
410 420 430 440 450
AIVQTLVHLL EPKKVPKPCC APTRLGALPV LYHLNDENVN LKKYRNMIVK

SCGCH
Length:455
Mass (Da):51,687
Last modified:August 1, 1992 - v1
Checksum:iC8FA795556341F94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77012 Genomic DNA. Translation: AAA28306.1.
M84795 mRNA. Translation: AAA28307.1.
AE013599 Genomic DNA. Translation: AAF47075.1.
PIRiA43918.
RefSeqiNP_001286786.1. NM_001299857.1.
NP_477340.1. NM_057992.3.
UniGeneiDm.4758.

Genome annotation databases

EnsemblMetazoaiFBtr0072127; FBpp0072036; FBgn0024234.
FBtr0343274; FBpp0309939; FBgn0024234.
GeneIDi37778.
KEGGidme:Dmel_CG5562.

Similar proteinsi

Entry informationi

Entry namei60A_DROME
AccessioniPrimary (citable) accession number: P27091
Secondary accession number(s): Q9W1I4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 25, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families