Reviewed, UniProtKB/Swiss-Prot P27054 (CHI4_PHAVU)

Names and origin

Protein names

Recommended name:
    Endochitinase PR4
    EC=3.2.1.14

Gene names

Name:

CHI4

Organism

Phaseolus vulgaris (Kidney bean) (French bean)

Taxonomic identifier

3885 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 270	247	Endochitinase PR4		PRO_0000005315
Regions
	Domain	24 – 58	35	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	26 ↔ 34		By similarity
	Disulfide bond	28 ↔ 40		By similarity
	Disulfide bond	33 ↔ 47		By similarity
	Disulfide bond	51 ↔ 56		By similarity
	Disulfide bond	88 ↔ 137		By similarity
	Disulfide bond	150 ↔ 160		By similarity
	Disulfide bond	238 ↔ 270		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27054-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 033ECF32904622C1	FASTA	270	28,834
	10 20 30 40 50 60 MGNKLVLVLV AVALVMGPKN VSAQNCGCAE GLCCSQYGYC GTGEDYCGTG CQQGPCTTAS 70 80 90 100 110 120 PPPSNNVNAD ILTADFLNGI IDQADSGCAG KNFYTRDAFL SALNSYTDFG RVGSEDDSKR 130 140 150 160 170 180 EIAAAFAHFT HETGHFCYIE EIDGASKDYC DEESIAQYPC SSSKGYHGRG PIQLSWNFNY 190 200 210 220 230 240 GPAGSANNFD GLGAPETVSN DVVVSFKTAL WYWMQHVRPV INQGFGATIR AINGALECDG 250 260 270 ANPTTVQARV NYYTEYCRQL GVATGDNLTC

References

[1]	"Isolation of a complementary DNA encoding the bean PR4 chitinase: an acidic enzyme with an amino-terminus cysteine-rich domain." Margis-Pinheiro M., Metz-Boutigue M.-H., Awade A., de Tapia M., le Ret M., Burkard G. Plant Mol. Biol. 17:243-253(1991) [PubMed: 1863776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Voegeli-Lange R. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57187 mRNA. Translation: CAA40474.1.
PIR	S16579.
3D structure databases
SMR	P27054. Positions 23-59.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 8.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI4_PHAVU

Accession

Primary (citable) accession number: P27054

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)