##gff-version 3
P27051	UniProtKB	Signal peptide	1	27	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255	
P27051	UniProtKB	Chain	28	243	.	.	.	ID=PRO_0000011788;Note=Beta-glucanase	
P27051	UniProtKB	Domain	29	243	.	.	.	Note=GH16;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01098	
P27051	UniProtKB	Active site	134	134	.	.	.	Note=Nucleophile	
P27051	UniProtKB	Active site	138	138	.	.	.	Note=Proton donor	
P27051	UniProtKB	Disulfide bond	61	90	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7589539;Dbxref=PMID:7589539	
P27051	UniProtKB	Mutagenesis	51	51	.	.	.	Note=30%25 of wild-type activity. D->N	
P27051	UniProtKB	Mutagenesis	89	89	.	.	.	Note=85%25 of wild-type activity. D->N	
P27051	UniProtKB	Mutagenesis	92	92	.	.	.	Note=3%25 of wild-type activity. E->Q	
P27051	UniProtKB	Mutagenesis	105	105	.	.	.	Note=50%25 of wild-type activity. E->Q	
P27051	UniProtKB	Mutagenesis	133	133	.	.	.	Note=15%25 of wild-type activity. D->N	
P27051	UniProtKB	Mutagenesis	134	134	.	.	.	Note=0.2%25 of wild-type activity. E->Q	
P27051	UniProtKB	Mutagenesis	136	136	.	.	.	Note=0.5%25 of wild-type activity. D->N	
P27051	UniProtKB	Mutagenesis	138	138	.	.	.	Note=Complete loss of activity. E->Q	
P27051	UniProtKB	Mutagenesis	143	143	.	.	.	Note=65%25 of wild-type activity. D->N	
P27051	UniProtKB	Mutagenesis	160	160	.	.	.	Note=15%25 of wild-type activity. E->Q	
P27051	UniProtKB	Mutagenesis	168	168	.	.	.	Note=60%25 of wild-type activity. D->N	
P27051	UniProtKB	Mutagenesis	179	179	.	.	.	Note=80%25 of wild-type activity. D->N	
P27051	UniProtKB	Mutagenesis	190	190	.	.	.	Note=70%25 of wild-type activity. D->N	
P27051	UniProtKB	Mutagenesis	219	219	.	.	.	Note=No change in activity. D->N	
P27051	UniProtKB	Sequence conflict	232	232	.	.	.	Note=S->Y;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P27051	UniProtKB	Beta strand	35	37	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Turn	44	46	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	47	49	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	61	63	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Helix	65	67	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	68	70	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	76	84	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	87	97	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	101	109	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	116	124	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Helix	126	128	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	133	140	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	146	153	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	161	164	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Turn	169	171	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	174	181	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	184	189	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	192	197	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	206	217	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Helix	219	222	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG	
P27051	UniProtKB	Beta strand	231	242	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1GBG