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Protein

Beta-glucanase

Gene

bg1

Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei134Nucleophile1
Active sitei138Proton donor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.73. 669.
SABIO-RKP27051.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene namesi
Name:bg1
OrganismiBacillus licheniformis
Taxonomic identifieri1402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51D → N: 30% of wild-type activity. 1
Mutagenesisi89D → N: 85% of wild-type activity. 1
Mutagenesisi92E → Q: 3% of wild-type activity. 1
Mutagenesisi105E → Q: 50% of wild-type activity. 1
Mutagenesisi133D → N: 15% of wild-type activity. 1
Mutagenesisi134E → Q: 0.2% of wild-type activity. 1
Mutagenesisi136D → N: 0.5% of wild-type activity. 1
Mutagenesisi138E → Q: Complete loss of activity. 1
Mutagenesisi143D → N: 65% of wild-type activity. 1
Mutagenesisi160E → Q: 15% of wild-type activity. 1
Mutagenesisi168D → N: 60% of wild-type activity. 1
Mutagenesisi179D → N: 80% of wild-type activity. 1
Mutagenesisi190D → N: 70% of wild-type activity. 1
Mutagenesisi219D → N: No change in activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001178828 – 243Beta-glucanaseAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi61 ↔ 901 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 37Combined sources3
Turni44 – 46Combined sources3
Beta strandi47 – 49Combined sources3
Beta strandi61 – 63Combined sources3
Helixi65 – 67Combined sources3
Beta strandi68 – 70Combined sources3
Beta strandi76 – 84Combined sources9
Beta strandi87 – 97Combined sources11
Beta strandi101 – 109Combined sources9
Beta strandi116 – 124Combined sources9
Helixi126 – 128Combined sources3
Beta strandi133 – 140Combined sources8
Beta strandi146 – 153Combined sources8
Beta strandi161 – 164Combined sources4
Turni169 – 171Combined sources3
Beta strandi174 – 181Combined sources8
Beta strandi184 – 189Combined sources6
Beta strandi192 – 197Combined sources6
Beta strandi206 – 217Combined sources12
Helixi219 – 222Combined sources4
Beta strandi231 – 242Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GBGX-ray1.80A30-243[»]
3D6EX-ray2.40A/B30-243[»]
ProteinModelPortaliP27051.
SMRiP27051.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27051.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 243GH16PROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYRVKRMLM LLVTGLFLSL STFAASASAQ TGGSFYEPFN NYNTGLWQKA
60 70 80 90 100
DGYSNGNMFN CTWRANNVSM TSLGEMRLSL TSPSYNKFDC GENRSVQTYG
110 120 130 140 150
YGLYEVNMKP AKNVGIVSSF FTYTGPTDGT PWDEIDIEFL GKDTTKVQFN
160 170 180 190 200
YYTNGVGNHE KIVNLGFDAA NSYHTYAFDW QPNSIKWYVD GQLKHTATTQ
210 220 230 240
IPQTPGKIMM NLWNGAGVDE WLGSYNGVTP LSRSLHWVRY TKR
Length:243
Mass (Da):27,435
Last modified:August 1, 1992 - v1
Checksum:i651188D9AAD609A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232S → Y (PubMed:7589539).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57279 Genomic DNA. Translation: CAA40547.1.
PIRiS15388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57279 Genomic DNA. Translation: CAA40547.1.
PIRiS15388.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GBGX-ray1.80A30-243[»]
3D6EX-ray2.40A/B30-243[»]
ProteinModelPortaliP27051.
SMRiP27051.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.73. 669.
SABIO-RKP27051.

Miscellaneous databases

EvolutionaryTraceiP27051.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUB_BACLI
AccessioniPrimary (citable) accession number: P27051
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.