Reviewed, UniProtKB/Swiss-Prot P27050 (CHID_BACCI)

Names and origin

Protein names

Recommended name:
    Chitinase D
    EC=3.2.1.14

Gene names

Name:

chiD

Organism

Bacillus circulans

Taxonomic identifier

1397 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. Contains 1 fibronectin type-III domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Ref.1
	Chain	31 – 524	494	Chitinase D		PRO_0000011906
Regions
	Domain	92 – 177	86	Fibronectin type-III
	Region	190 – 524	335	Catalytic
Sites
	Active site	303	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27050-1 [UniParc]. Last modified May 30, 2000. Version 4. Checksum: 208D714902A741E6	FASTA	524	55,760
	10 20 30 40 50 60 MNQAVRFRPV ITFALAFILI ITWFAPRADA AAQWQAGTAY KQGDLVTYLN KDYECIQPHT 70 80 90 100 110 120 ALTGWEPSNV PALWKYVGEG TGGGTPTPDT TPPTVPAGLT SSLVTDTSVN LTWNASTDNV 130 140 150 160 170 180 GVTGYEVYRN GTLVANTSTT TAVVTGLTAG TTYVFTVKAK DAAGNLSAAS TSLSVTTSTG 190 200 210 220 230 240 SSNPGPSGSK WLIGYWHNFD NGSTNIKLRN VSTAYDVINV SFAEPISPGS GTLAFTPYNA 250 260 270 280 290 300 TVEEFKSDIA YLQSQGKKVL ISMGGANGRI ELTDATKKRQ QFEDSLKSII STYGFNGLDI 310 320 330 340 350 360 DLEGSSLSLN AGDTDFRSPT TPKIVNLING VKALKSHFGA NFVLTAAPET AYVQGGYLNY 370 380 390 400 410 420 GGPWGAYLPV IHALRNDLTL LHVQHYNTGS MVGLDGRSYA QGTADFHVAM AQMLLQGFNV 430 440 450 460 470 480 GGSSGPFFSP LRPDQIAIGV PASQQAAGGG YTAPAELQKA LNYLIKGVSY GGSYTLRQPA 490 500 510 520 GYVGLKGIMT WSINWDAYTN NQFSNAHRPF LNGLSTQKTE EVVY

References

[1]	"Structure of the gene encoding chitinase D of Bacillus circulans WL-12 and possible homology of the enzyme to other prokaryotic chitinases and class III plant chitinases." Watanabe T., Oyanagi W., Suzuki K., Ohnishi K., Tanaka H. J. Bacteriol. 174:408-414(1992) [PubMed: 1729234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-42. Strain: WL-12.
[2]	Watanabe T. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10594 Genomic DNA. Translation: BAA34114.1.
PIR	A41961.
3D structure databases
SMR	P27050. Positions 34-75, 96-187, 192-507.
ModBase	Search...
Protein family/group databases
CAZy	CBM12. Carbohydrate-Binding Module Family 12. GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 1207.
Family and domain databases
InterPro	IPR003610. Carb-bd_dom_fam5/12. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR015520. Imaginal_disc_gf. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR11177:SF25. Imaginal_disc_gf. 1 hit.
Pfam	PF02839. CBM_5_12. 1 hit. PF00041. fn3. 1 hit. PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PRINTS	PR00014. FNTYPEIII.
SMART	SM00495. ChtBD3. 1 hit. SM00060. FN3. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. PS50853. FN3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHID_BACCI

Accession

Primary (citable) accession number: P27050

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	May 30, 2000
Last modified:	January 19, 2010
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)