P27049 (BTG2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BTG2

Alternative name(s):
BTG family member 2
NGF-inducible anti-proliferative protein PC3

Gene names

Name:	Btg2
Synonyms:	Pc3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth By similarity. Anti-proliferative protein. Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors.
Subunit structure	Binds the CCR4-NOT complex By similarity. Interacts with PRKCABP.Interacts with PIN1, inducing mitochondrial depolarization By similarity. Ref.5
Tissue specificity	In brain at embryonic day 13.5, placenta, amnion, and spleen, which are proliferating and/or differentiating.
Induction	By nerve growth factor. At lower levels by epidermal growth factor and membrane depolarization. At much lower levels by fibroblast growth factor and interleukin 6.
Post-translational modification	Phosphorylated at Ser-147 by MAPK1/ERK2 and MAPK3/ERK1, and at Ser-149 by MAPK14, leading to PIN1-binding and mitochondrial depolarization By similarity.
Sequence similarities	Belongs to the BTG family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of apoptotic process Inferred from direct assay. Source: MGI positive regulation of anti-apoptosis Inferred from mutant phenotype. Source: RGD response to electrical stimulus Inferred from expression pattern. Source: RGD response to mechanical stimulus Inferred from expression pattern. Source: RGD response to organic cyclic compound Inferred from expression pattern. Source: RGD response to organic nitrogen Inferred from expression pattern. Source: RGD response to peptide hormone stimulus Inferred from expression pattern. Source: RGD
Molecular function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pick1	Q9EP80	3	EBI-78953,EBI-77728

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 158	158	Protein BTG2		PRO_0000143806
Amino acid modifications
	Modified residue	147	1	Phosphoserine; by MAPK1 and MAPK3 By similarity
	Modified residue	149	1	Phosphoserine; by MAPK14 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27049 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: F9105D73C3CDF24A	FASTA	158	17,728
	10 20 30 40 50 60 MSHGKRTDML PEIAAAVGFL TSLLRTRGCV SEQRLKVFSR ALQDALTDHY KHHWFPEKPS 70 80 90 100 110 120 KGSGYRCIRI NHKMDPIISK VASQIGLSQP QLHQLLPSEL TLWVDPYEVS YRIGEDGSIC 130 140 150 VLYEEAPVAT SYGLLTCKNQ MMLGRSSPSK NYVMTVSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of PC3, a putatively secreted protein whose mRNA is induced by nerve growth factor and depolarization." Bradbury A., Possenti R., Shooter E.M., Tirone F. Proc. Natl. Acad. Sci. U.S.A. 88:3353-3357(1991) [PubMed: 1849653] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Developmental expression of PC3 gene is correlated with neuronal cell birthday." Iacopetti P., Barsacchi G., Tirone F., Maffei L., Cremisi F. Mech. Dev. 47:127-137(1994) [PubMed: 7811636] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Overexpression of the nerve growth factor-inducible PC3 immediate early gene is associated with growth inhibition." Montagnoli A., Guardavaccaro D., Starace G., Tirone F. Cell Growth Differ. 7:1327-1336(1996) [PubMed: 8891336] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Mitogen-stimulated TIS21 protein interacts with a protein-kinase-Calpha-binding protein rPICK1." Lin W.-J., Chang Y.-F., Wang W.-L., Huang C.-Y.F. Biochem. J. 354:635-643(2001) [PubMed: 11237868] [Abstract] Cited for: INTERACTION WITH PRKCABP.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60921 mRNA. Translation: AAB49567.1. BC072493 mRNA. Translation: AAH72493.1.
IPI	IPI00193262.
PIR	A40443.
RefSeq	NP_058955.1. NM_017259.1.
UniGene	Rn.27923.
3D structure databases
ProteinModelPortal	P27049.
ModBase	Search...
Protein-protein interaction databases
IntAct	P27049. 2 interactions.
STRING	P27049.
Proteomic databases
PRIDE	P27049.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000004408; ENSRNOP00000004408; ENSRNOG00000003300.
GeneID	29619.
KEGG	rno:29619.
UCSC	BC072493. rat.
Organism-specific databases
CTD	7832.
RGD	2225. Btg2.
Phylogenomic databases
eggNOG	maNOG17325.
GeneTree	ENSGT00550000074461.
HOVERGEN	HBG004907.
InParanoid	P27049.
OMA	PSKNYIM.
OrthoDB	EOG4P8FKB.
PhylomeDB	P27049.
Gene expression databases
ArrayExpress	P27049.
Genevestigator	P27049.
GermOnline	ENSRNOG00000003300. Rattus norvegicus.
Family and domain databases
InterPro	IPR002087. Anti_prolifrtn. [Graphical view]
KO	K14443.
Pfam	PF07742. BTG. 1 hit. [Graphical view]
PRINTS	PR00310. ANTIPRLFBTG1.
SMART	SM00099. btg1. 1 hit. [Graphical view]
PROSITE	PS00960. BTG_1. 1 hit. PS01203. BTG_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	609818.

Entry information

Entry name

BTG2_RAT

Accession

Primary (citable) accession number: P27049

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program