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P27046 (MA2A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-mannosidase 2

EC=3.2.1.114
Alternative name(s):
Golgi alpha-mannosidase II
Short name=AMan II
Short name=Man II
Mannosidase alpha class 2A member 1
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene names
Name:Man2a1
Synonyms:Mana2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1150 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

Catalytic activity

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Homodimer; disulfide-linked.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Tissue specificity

All tissues, mostly in adrenal and thymus.

Post-translational modification

Glycosylated By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processN-glycan processing

Inferred from genetic interaction PubMed 16754854. Source: MGI

in utero embryonic development

Inferred from genetic interaction PubMed 16754854. Source: MGI

liver development

Inferred from genetic interaction PubMed 16754854. Source: MGI

lung alveolus development

Inferred from genetic interaction PubMed 16754854. Source: MGI

mannose metabolic process

Inferred from electronic annotation. Source: InterPro

mitochondrion organization

Inferred from genetic interaction PubMed 16754854. Source: MGI

positive regulation of neurogenesis

Inferred from mutant phenotype PubMed 20144606. Source: MGI

respiratory gaseous exchange

Inferred from genetic interaction PubMed 16754854. Source: MGI

retina morphogenesis in camera-type eye

Inferred from mutant phenotype PubMed 20144606. Source: MGI

vacuole organization

Inferred from genetic interaction PubMed 16754854. Source: MGI

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 16204232PubMed 23000399. Source: MGI

Golgi membrane

Inferred from direct assay PubMed 11042173. Source: MGI

cis-Golgi network

Inferred from direct assay PubMed 22841714. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

hydrolase activity, hydrolyzing N-glycosyl compounds

Inferred from direct assay PubMed 16754854. Source: MGI

mannosidase activity

Inferred from direct assay PubMed 16754854. Source: MGI

mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11501150Alpha-mannosidase 2
PRO_0000206903

Regions

Topological domain1 – 55Cytoplasmic Potential
Transmembrane6 – 2621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain27 – 11501124Lumenal Potential

Sites

Active site2881Nucleophile By similarity
Metal binding1741Zinc By similarity
Metal binding1761Zinc By similarity
Metal binding2881Zinc By similarity
Metal binding5681Zinc By similarity

Amino acid modifications

Modified residue801Phosphoserine Ref.3
Modified residue821Phosphoserine Ref.3
Glycosylation781N-linked (GlcNAc...) Potential
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation11291N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict6731V → G in CAA43480. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P27046 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 5CDD9DD388DF5BC6

FASTA1,150131,631
        10         20         30         40         50         60 
MKLSRQFTVF GSAIFCVVIF SLYLMLDRGH LDYPRGPRQE GSFPQGQLSI LQEKIDHLER 

        70         80         90        100        110        120 
LLAENNEIIS NIRDSVINLS ESVEDGPRGS PGNASQGSIH LHSPQLALQA DPRDCLFASQ 

       130        140        150        160        170        180 
SGSQPRDVQM LDVYDLIPFD NPDGGVWKQG FDIKYEADEW DHEPLQVFVV PHSHNDPGWL 

       190        200        210        220        230        240 
KTFNDYFRDK TQYIFNNMVL KLKEDSSRKF MWSEISYLAK WWDIIDIPKK EAVKSLLQNG 

       250        260        270        280        290        300 
QLEIVTGGWV MPDEATPHYF ALIDQLIEGH QWLEKNLGVK PRSGWAIDPF GHSPTMAYLL 

       310        320        330        340        350        360 
KRAGFSHMLI QRVHYAIKKH FSLHKTLEFF WRQNWDLGSA TDILCHMMPF YSYDIPHTCG 

       370        380        390        400        410        420 
PDPKICCQFD FKRLPGGRYG CPWGVPPEAI SPGNVQSRAQ MLLDQYRKKS KLFRTKVLLA 

       430        440        450        460        470        480 
PLGDDFRFSE YTEWDLQCRN YEQLFSYMNS QPHLKVKIQF GTLSDYFDAL EKAVAAEKKS 

       490        500        510        520        530        540 
SQSVFPALSG DFFTYADRDD HYWSGYFTSR PFYKRMDRIM ESRIRAAEIL YQLALKQAQK 

       550        560        570        580        590        600 
YKINKFLSSP HYTTLTEARR NLGLFQHHDA ITGTAKDWVV VDYGTRLFQS LNSLEKIIGD 

       610        620        630        640        650        660 
SAFLLILKDK KLYQSDPSKA FLEMDTKQSS QDSLPQKIII QLSAQEPRYL VVYNPFEQER 

       670        680        690        700        710        720 
HSVVSIRVNS ATVKVLSDSG KPVEVQVSAV WNDMRTISQA AYEVSFLAHI PPLGLKVFKI 

       730        740        750        760        770        780 
LESQSSSSHL ADYVLYNNDG LAENGIFHVK NMVDAGDAIT IENPFLAIWF DRSGLMEKVR 

       790        800        810        820        830        840 
RKEDSRQHEL KVQFLWYGTT NKRDKSGAYL FLPDGQGQPY VSLRPPFVRV TRGRIYSDVT 

       850        860        870        880        890        900 
CFLEHVTHKV RLYNIQGIEG QSMEVSNIVN IRNVHNREIV MRISSKINNQ NRYYTDLNGY 

       910        920        930        940        950        960 
QIQPRRTMSK LPLQANVYPM CTMAYIQDAE HRLTLLSAQS LGASSMASGQ IEVFMDRRLM 

       970        980        990       1000       1010       1020 
QDDNRGLGQG VHDNKITANL FRILLEKRSA VNMEEEKKSP VSYPSLLSHM TSSFLNHPFL 

      1030       1040       1050       1060       1070       1080 
PMVLSGQLPS PAFELLSEFP LLQSSLPCDI HLVNLRTIQS KMGKGYSDEA ALILHRKGFD 

      1090       1100       1110       1120       1130       1140 
CQFSSRGIGL PCSTTQGKMS VLKLFNKFAV ESLVPSSLSL MHSPPDAQNM SEVSLSPMEI 

      1150 
STFRIRLRWT 

« Hide

References

« Hide 'large scale' references
[1]"Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans."
Moremen K.W., Robbins P.W.
J. Cell Biol. 115:1521-1534(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: BALB/c.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61172 mRNA. Translation: CAA43480.1.
BC138371 mRNA. Translation: AAI38372.1.
BC138372 mRNA. Translation: AAI38373.1.
CCDSCCDS28938.1.
PIRA41641.
RefSeqNP_032575.2. NM_008549.2.
UniGeneMm.2433.

3D structure databases

ProteinModelPortalP27046.
SMRP27046. Positions 127-1019.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP27046. 1 interaction.
MINTMINT-4996610.

Protein family/group databases

CAZyGH38. Glycoside Hydrolase Family 38.

PTM databases

PhosphoSiteP27046.

Proteomic databases

MaxQBP27046.
PaxDbP27046.
PRIDEP27046.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000086723; ENSMUSP00000083928; ENSMUSG00000024085.
GeneID17158.
KEGGmmu:17158.
UCSCuc008dfy.1. mouse.

Organism-specific databases

CTD4124.
MGIMGI:104669. Man2a1.

Phylogenomic databases

eggNOGCOG0383.
GeneTreeENSGT00510000046304.
HOGENOMHOG000293253.
HOVERGENHBG052390.
InParanoidP27046.
KOK01231.
OMAPRMTMSK.
OrthoDBEOG7JQBMJ.
TreeFamTF313152.

Enzyme and pathway databases

BRENDA3.2.1.114. 3474.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressP27046.
BgeeP27046.
CleanExMM_MAN2A1.
GenevestigatorP27046.

Family and domain databases

Gene3D1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Other

ChiTaRSMAN2A1. mouse.
NextBio291426.
PROP27046.
SOURCESearch...

Entry information

Entry nameMA2A1_MOUSE
AccessionPrimary (citable) accession number: P27046
Secondary accession number(s): B9EHS6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries