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P27046

- MA2A1_MOUSE

UniProt

P27046 - MA2A1_MOUSE

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Protein

Alpha-mannosidase 2

Gene
Man2a1, Mana2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

Catalytic activityi

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi174 – 1741Zinc By similarity
Metal bindingi176 – 1761Zinc By similarity
Active sitei288 – 2881Nucleophile By similarity
Metal bindingi288 – 2881Zinc By similarity
Metal bindingi568 – 5681Zinc By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. hydrolase activity, hydrolyzing N-glycosyl compounds Source: MGI
  3. mannosidase activity Source: MGI
  4. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: UniProtKB-EC
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. in utero embryonic development Source: MGI
  2. liver development Source: MGI
  3. lung alveolus development Source: MGI
  4. mannose metabolic process Source: InterPro
  5. mitochondrion organization Source: MGI
  6. N-glycan processing Source: MGI
  7. positive regulation of neurogenesis Source: MGI
  8. respiratory gaseous exchange Source: MGI
  9. retina morphogenesis in camera-type eye Source: MGI
  10. vacuole organization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.2.1.114. 3474.
ReactomeiREACT_198568. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase 2 (EC:3.2.1.114)
Alternative name(s):
Golgi alpha-mannosidase II
Short name:
AMan II
Short name:
Man II
Mannosidase alpha class 2A member 1
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene namesi
Name:Man2a1
Synonyms:Mana2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:104669. Man2a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Cytoplasmic Reviewed prediction
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini27 – 11501124Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cis-Golgi network Source: MGI
  2. Golgi apparatus Source: MGI
  3. Golgi membrane Source: MGI
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11501150Alpha-mannosidase 2PRO_0000206903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...) Reviewed prediction
Modified residuei80 – 801Phosphoserine1 Publication
Modified residuei82 – 821Phosphoserine1 Publication
Glycosylationi93 – 931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1129 – 11291N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP27046.
PaxDbiP27046.
PRIDEiP27046.

PTM databases

PhosphoSiteiP27046.

Expressioni

Tissue specificityi

All tissues, mostly in adrenal and thymus.

Gene expression databases

ArrayExpressiP27046.
BgeeiP27046.
CleanExiMM_MAN2A1.
GenevestigatoriP27046.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

IntActiP27046. 1 interaction.
MINTiMINT-4996610.

Structurei

3D structure databases

ProteinModelPortaliP27046.
SMRiP27046. Positions 127-1019.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0383.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000293253.
HOVERGENiHBG052390.
InParanoidiP27046.
KOiK01231.
OMAiPRMTMSK.
OrthoDBiEOG7JQBMJ.
TreeFamiTF313152.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

P27046-1 [UniParc]FASTAAdd to Basket

« Hide

MKLSRQFTVF GSAIFCVVIF SLYLMLDRGH LDYPRGPRQE GSFPQGQLSI     50
LQEKIDHLER LLAENNEIIS NIRDSVINLS ESVEDGPRGS PGNASQGSIH 100
LHSPQLALQA DPRDCLFASQ SGSQPRDVQM LDVYDLIPFD NPDGGVWKQG 150
FDIKYEADEW DHEPLQVFVV PHSHNDPGWL KTFNDYFRDK TQYIFNNMVL 200
KLKEDSSRKF MWSEISYLAK WWDIIDIPKK EAVKSLLQNG QLEIVTGGWV 250
MPDEATPHYF ALIDQLIEGH QWLEKNLGVK PRSGWAIDPF GHSPTMAYLL 300
KRAGFSHMLI QRVHYAIKKH FSLHKTLEFF WRQNWDLGSA TDILCHMMPF 350
YSYDIPHTCG PDPKICCQFD FKRLPGGRYG CPWGVPPEAI SPGNVQSRAQ 400
MLLDQYRKKS KLFRTKVLLA PLGDDFRFSE YTEWDLQCRN YEQLFSYMNS 450
QPHLKVKIQF GTLSDYFDAL EKAVAAEKKS SQSVFPALSG DFFTYADRDD 500
HYWSGYFTSR PFYKRMDRIM ESRIRAAEIL YQLALKQAQK YKINKFLSSP 550
HYTTLTEARR NLGLFQHHDA ITGTAKDWVV VDYGTRLFQS LNSLEKIIGD 600
SAFLLILKDK KLYQSDPSKA FLEMDTKQSS QDSLPQKIII QLSAQEPRYL 650
VVYNPFEQER HSVVSIRVNS ATVKVLSDSG KPVEVQVSAV WNDMRTISQA 700
AYEVSFLAHI PPLGLKVFKI LESQSSSSHL ADYVLYNNDG LAENGIFHVK 750
NMVDAGDAIT IENPFLAIWF DRSGLMEKVR RKEDSRQHEL KVQFLWYGTT 800
NKRDKSGAYL FLPDGQGQPY VSLRPPFVRV TRGRIYSDVT CFLEHVTHKV 850
RLYNIQGIEG QSMEVSNIVN IRNVHNREIV MRISSKINNQ NRYYTDLNGY 900
QIQPRRTMSK LPLQANVYPM CTMAYIQDAE HRLTLLSAQS LGASSMASGQ 950
IEVFMDRRLM QDDNRGLGQG VHDNKITANL FRILLEKRSA VNMEEEKKSP 1000
VSYPSLLSHM TSSFLNHPFL PMVLSGQLPS PAFELLSEFP LLQSSLPCDI 1050
HLVNLRTIQS KMGKGYSDEA ALILHRKGFD CQFSSRGIGL PCSTTQGKMS 1100
VLKLFNKFAV ESLVPSSLSL MHSPPDAQNM SEVSLSPMEI STFRIRLRWT 1150
Length:1,150
Mass (Da):131,631
Last modified:July 27, 2011 - v2
Checksum:i5CDD9DD388DF5BC6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti673 – 6731V → G in CAA43480. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61172 mRNA. Translation: CAA43480.1.
BC138371 mRNA. Translation: AAI38372.1.
BC138372 mRNA. Translation: AAI38373.1.
CCDSiCCDS28938.1.
PIRiA41641.
RefSeqiNP_032575.2. NM_008549.2.
UniGeneiMm.2433.

Genome annotation databases

EnsembliENSMUST00000086723; ENSMUSP00000083928; ENSMUSG00000024085.
GeneIDi17158.
KEGGimmu:17158.
UCSCiuc008dfy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61172 mRNA. Translation: CAA43480.1 .
BC138371 mRNA. Translation: AAI38372.1 .
BC138372 mRNA. Translation: AAI38373.1 .
CCDSi CCDS28938.1.
PIRi A41641.
RefSeqi NP_032575.2. NM_008549.2.
UniGenei Mm.2433.

3D structure databases

ProteinModelPortali P27046.
SMRi P27046. Positions 127-1019.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P27046. 1 interaction.
MINTi MINT-4996610.

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

PTM databases

PhosphoSitei P27046.

Proteomic databases

MaxQBi P27046.
PaxDbi P27046.
PRIDEi P27046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000086723 ; ENSMUSP00000083928 ; ENSMUSG00000024085 .
GeneIDi 17158.
KEGGi mmu:17158.
UCSCi uc008dfy.1. mouse.

Organism-specific databases

CTDi 4124.
MGIi MGI:104669. Man2a1.

Phylogenomic databases

eggNOGi COG0383.
GeneTreei ENSGT00510000046304.
HOGENOMi HOG000293253.
HOVERGENi HBG052390.
InParanoidi P27046.
KOi K01231.
OMAi PRMTMSK.
OrthoDBi EOG7JQBMJ.
TreeFami TF313152.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 3.2.1.114. 3474.
Reactomei REACT_198568. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

ChiTaRSi MAN2A1. mouse.
NextBioi 291426.
PROi P27046.
SOURCEi Search...

Gene expression databases

ArrayExpressi P27046.
Bgeei P27046.
CleanExi MM_MAN2A1.
Genevestigatori P27046.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans."
    Moremen K.W., Robbins P.W.
    J. Cell Biol. 115:1521-1534(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMA2A1_MOUSE
AccessioniPrimary (citable) accession number: P27046
Secondary accession number(s): B9EHS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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