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P27046

- MA2A1_MOUSE

UniProt

P27046 - MA2A1_MOUSE

Protein

Alpha-mannosidase 2

Gene

Man2a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

    Catalytic activityi

    Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi174 – 1741ZincBy similarity
    Metal bindingi176 – 1761ZincBy similarity
    Active sitei288 – 2881NucleophileBy similarity
    Metal bindingi288 – 2881ZincBy similarity
    Metal bindingi568 – 5681ZincBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. hydrolase activity, hydrolyzing N-glycosyl compounds Source: MGI
    3. mannosidase activity Source: MGI
    4. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: UniProtKB-EC
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. in utero embryonic development Source: MGI
    2. liver development Source: MGI
    3. lung alveolus development Source: MGI
    4. mannose metabolic process Source: InterPro
    5. mitochondrion organization Source: MGI
    6. N-glycan processing Source: MGI
    7. positive regulation of neurogenesis Source: MGI
    8. respiratory gaseous exchange Source: MGI
    9. retina morphogenesis in camera-type eye Source: MGI
    10. vacuole organization Source: MGI

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.2.1.114. 3474.
    ReactomeiREACT_198568. Reactions specific to the complex N-glycan synthesis pathway.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH38. Glycoside Hydrolase Family 38.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-mannosidase 2 (EC:3.2.1.114)
    Alternative name(s):
    Golgi alpha-mannosidase II
    Short name:
    AMan II
    Short name:
    Man II
    Mannosidase alpha class 2A member 1
    Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
    Gene namesi
    Name:Man2a1
    Synonyms:Mana2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:104669. Man2a1.

    Subcellular locationi

    GO - Cellular componenti

    1. cis-Golgi network Source: MGI
    2. Golgi apparatus Source: MGI
    3. Golgi membrane Source: MGI
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11501150Alpha-mannosidase 2PRO_0000206903Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
    Modified residuei80 – 801Phosphoserine1 Publication
    Modified residuei82 – 821Phosphoserine1 Publication
    Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1129 – 11291N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP27046.
    PaxDbiP27046.
    PRIDEiP27046.

    PTM databases

    PhosphoSiteiP27046.

    Expressioni

    Tissue specificityi

    All tissues, mostly in adrenal and thymus.

    Gene expression databases

    ArrayExpressiP27046.
    BgeeiP27046.
    CleanExiMM_MAN2A1.
    GenevestigatoriP27046.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.

    Protein-protein interaction databases

    IntActiP27046. 1 interaction.
    MINTiMINT-4996610.

    Structurei

    3D structure databases

    ProteinModelPortaliP27046.
    SMRiP27046. Positions 127-1019.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 55CytoplasmicSequence Analysis
    Topological domaini27 – 11501124LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0383.
    GeneTreeiENSGT00510000046304.
    HOGENOMiHOG000293253.
    HOVERGENiHBG052390.
    InParanoidiP27046.
    KOiK01231.
    OMAiPRMTMSK.
    OrthoDBiEOG7JQBMJ.
    TreeFamiTF313152.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view]
    SMARTiSM00872. Alpha-mann_mid. 1 hit.
    [Graphical view]
    SUPFAMiSSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P27046-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSRQFTVF GSAIFCVVIF SLYLMLDRGH LDYPRGPRQE GSFPQGQLSI     50
    LQEKIDHLER LLAENNEIIS NIRDSVINLS ESVEDGPRGS PGNASQGSIH 100
    LHSPQLALQA DPRDCLFASQ SGSQPRDVQM LDVYDLIPFD NPDGGVWKQG 150
    FDIKYEADEW DHEPLQVFVV PHSHNDPGWL KTFNDYFRDK TQYIFNNMVL 200
    KLKEDSSRKF MWSEISYLAK WWDIIDIPKK EAVKSLLQNG QLEIVTGGWV 250
    MPDEATPHYF ALIDQLIEGH QWLEKNLGVK PRSGWAIDPF GHSPTMAYLL 300
    KRAGFSHMLI QRVHYAIKKH FSLHKTLEFF WRQNWDLGSA TDILCHMMPF 350
    YSYDIPHTCG PDPKICCQFD FKRLPGGRYG CPWGVPPEAI SPGNVQSRAQ 400
    MLLDQYRKKS KLFRTKVLLA PLGDDFRFSE YTEWDLQCRN YEQLFSYMNS 450
    QPHLKVKIQF GTLSDYFDAL EKAVAAEKKS SQSVFPALSG DFFTYADRDD 500
    HYWSGYFTSR PFYKRMDRIM ESRIRAAEIL YQLALKQAQK YKINKFLSSP 550
    HYTTLTEARR NLGLFQHHDA ITGTAKDWVV VDYGTRLFQS LNSLEKIIGD 600
    SAFLLILKDK KLYQSDPSKA FLEMDTKQSS QDSLPQKIII QLSAQEPRYL 650
    VVYNPFEQER HSVVSIRVNS ATVKVLSDSG KPVEVQVSAV WNDMRTISQA 700
    AYEVSFLAHI PPLGLKVFKI LESQSSSSHL ADYVLYNNDG LAENGIFHVK 750
    NMVDAGDAIT IENPFLAIWF DRSGLMEKVR RKEDSRQHEL KVQFLWYGTT 800
    NKRDKSGAYL FLPDGQGQPY VSLRPPFVRV TRGRIYSDVT CFLEHVTHKV 850
    RLYNIQGIEG QSMEVSNIVN IRNVHNREIV MRISSKINNQ NRYYTDLNGY 900
    QIQPRRTMSK LPLQANVYPM CTMAYIQDAE HRLTLLSAQS LGASSMASGQ 950
    IEVFMDRRLM QDDNRGLGQG VHDNKITANL FRILLEKRSA VNMEEEKKSP 1000
    VSYPSLLSHM TSSFLNHPFL PMVLSGQLPS PAFELLSEFP LLQSSLPCDI 1050
    HLVNLRTIQS KMGKGYSDEA ALILHRKGFD CQFSSRGIGL PCSTTQGKMS 1100
    VLKLFNKFAV ESLVPSSLSL MHSPPDAQNM SEVSLSPMEI STFRIRLRWT 1150
    Length:1,150
    Mass (Da):131,631
    Last modified:July 27, 2011 - v2
    Checksum:i5CDD9DD388DF5BC6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti673 – 6731V → G in CAA43480. (PubMed:1757461)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61172 mRNA. Translation: CAA43480.1.
    BC138371 mRNA. Translation: AAI38372.1.
    BC138372 mRNA. Translation: AAI38373.1.
    CCDSiCCDS28938.1.
    PIRiA41641.
    RefSeqiNP_032575.2. NM_008549.2.
    UniGeneiMm.2433.

    Genome annotation databases

    EnsembliENSMUST00000086723; ENSMUSP00000083928; ENSMUSG00000024085.
    GeneIDi17158.
    KEGGimmu:17158.
    UCSCiuc008dfy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61172 mRNA. Translation: CAA43480.1 .
    BC138371 mRNA. Translation: AAI38372.1 .
    BC138372 mRNA. Translation: AAI38373.1 .
    CCDSi CCDS28938.1.
    PIRi A41641.
    RefSeqi NP_032575.2. NM_008549.2.
    UniGenei Mm.2433.

    3D structure databases

    ProteinModelPortali P27046.
    SMRi P27046. Positions 127-1019.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P27046. 1 interaction.
    MINTi MINT-4996610.

    Protein family/group databases

    CAZyi GH38. Glycoside Hydrolase Family 38.

    PTM databases

    PhosphoSitei P27046.

    Proteomic databases

    MaxQBi P27046.
    PaxDbi P27046.
    PRIDEi P27046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000086723 ; ENSMUSP00000083928 ; ENSMUSG00000024085 .
    GeneIDi 17158.
    KEGGi mmu:17158.
    UCSCi uc008dfy.1. mouse.

    Organism-specific databases

    CTDi 4124.
    MGIi MGI:104669. Man2a1.

    Phylogenomic databases

    eggNOGi COG0383.
    GeneTreei ENSGT00510000046304.
    HOGENOMi HOG000293253.
    HOVERGENi HBG052390.
    InParanoidi P27046.
    KOi K01231.
    OMAi PRMTMSK.
    OrthoDBi EOG7JQBMJ.
    TreeFami TF313152.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 3.2.1.114. 3474.
    Reactomei REACT_198568. Reactions specific to the complex N-glycan synthesis pathway.

    Miscellaneous databases

    ChiTaRSi MAN2A1. mouse.
    NextBioi 291426.
    PROi P27046.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P27046.
    Bgeei P27046.
    CleanExi MM_MAN2A1.
    Genevestigatori P27046.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view ]
    SMARTi SM00872. Alpha-mann_mid. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans."
      Moremen K.W., Robbins P.W.
      J. Cell Biol. 115:1521-1534(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: BALB/c.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiMA2A1_MOUSE
    AccessioniPrimary (citable) accession number: P27046
    Secondary accession number(s): B9EHS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3