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Protein

Alpha-mannosidase 2

Gene

Man2a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

Catalytic activityi

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi174 – 1741ZincBy similarity
Metal bindingi176 – 1761ZincBy similarity
Active sitei288 – 2881NucleophileBy similarity
Metal bindingi288 – 2881ZincBy similarity
Metal bindingi568 – 5681ZincBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. hydrolase activity, hydrolyzing N-glycosyl compounds Source: MGI
  3. mannosidase activity Source: MGI
  4. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: UniProtKB-EC
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. in utero embryonic development Source: MGI
  2. liver development Source: MGI
  3. lung alveolus development Source: MGI
  4. mannose metabolic process Source: InterPro
  5. mitochondrion organization Source: MGI
  6. N-glycan processing Source: MGI
  7. positive regulation of neurogenesis Source: MGI
  8. respiratory gaseous exchange Source: MGI
  9. retina morphogenesis in camera-type eye Source: MGI
  10. vacuole organization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.2.1.114. 3474.
ReactomeiREACT_198568. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase 2 (EC:3.2.1.114)
Alternative name(s):
Golgi alpha-mannosidase II
Short name:
AMan II
Short name:
Man II
Mannosidase alpha class 2A member 1
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene namesi
Name:Man2a1
Synonyms:Mana2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:104669. Man2a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55CytoplasmicSequence Analysis
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini27 – 11501124LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cis-Golgi network Source: MGI
  2. extracellular vesicular exosome Source: MGI
  3. Golgi apparatus Source: MGI
  4. Golgi membrane Source: MGI
  5. integral component of membrane Source: UniProtKB-KW
  6. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11501150Alpha-mannosidase 2PRO_0000206903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
Modified residuei80 – 801Phosphoserine1 Publication
Modified residuei82 – 821Phosphoserine1 Publication
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1129 – 11291N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP27046.
PaxDbiP27046.
PRIDEiP27046.

PTM databases

PhosphoSiteiP27046.

Expressioni

Tissue specificityi

All tissues, mostly in adrenal and thymus.

Gene expression databases

BgeeiP27046.
CleanExiMM_MAN2A1.
ExpressionAtlasiP27046. baseline and differential.
GenevestigatoriP27046.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

IntActiP27046. 1 interaction.
MINTiMINT-4996610.

Structurei

3D structure databases

ProteinModelPortaliP27046.
SMRiP27046. Positions 127-1019.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0383.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000293253.
HOVERGENiHBG052390.
InParanoidiP27046.
KOiK01231.
OMAiFFEHVTH.
OrthoDBiEOG7JQBMJ.
TreeFamiTF313152.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

P27046-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLSRQFTVF GSAIFCVVIF SLYLMLDRGH LDYPRGPRQE GSFPQGQLSI
60 70 80 90 100
LQEKIDHLER LLAENNEIIS NIRDSVINLS ESVEDGPRGS PGNASQGSIH
110 120 130 140 150
LHSPQLALQA DPRDCLFASQ SGSQPRDVQM LDVYDLIPFD NPDGGVWKQG
160 170 180 190 200
FDIKYEADEW DHEPLQVFVV PHSHNDPGWL KTFNDYFRDK TQYIFNNMVL
210 220 230 240 250
KLKEDSSRKF MWSEISYLAK WWDIIDIPKK EAVKSLLQNG QLEIVTGGWV
260 270 280 290 300
MPDEATPHYF ALIDQLIEGH QWLEKNLGVK PRSGWAIDPF GHSPTMAYLL
310 320 330 340 350
KRAGFSHMLI QRVHYAIKKH FSLHKTLEFF WRQNWDLGSA TDILCHMMPF
360 370 380 390 400
YSYDIPHTCG PDPKICCQFD FKRLPGGRYG CPWGVPPEAI SPGNVQSRAQ
410 420 430 440 450
MLLDQYRKKS KLFRTKVLLA PLGDDFRFSE YTEWDLQCRN YEQLFSYMNS
460 470 480 490 500
QPHLKVKIQF GTLSDYFDAL EKAVAAEKKS SQSVFPALSG DFFTYADRDD
510 520 530 540 550
HYWSGYFTSR PFYKRMDRIM ESRIRAAEIL YQLALKQAQK YKINKFLSSP
560 570 580 590 600
HYTTLTEARR NLGLFQHHDA ITGTAKDWVV VDYGTRLFQS LNSLEKIIGD
610 620 630 640 650
SAFLLILKDK KLYQSDPSKA FLEMDTKQSS QDSLPQKIII QLSAQEPRYL
660 670 680 690 700
VVYNPFEQER HSVVSIRVNS ATVKVLSDSG KPVEVQVSAV WNDMRTISQA
710 720 730 740 750
AYEVSFLAHI PPLGLKVFKI LESQSSSSHL ADYVLYNNDG LAENGIFHVK
760 770 780 790 800
NMVDAGDAIT IENPFLAIWF DRSGLMEKVR RKEDSRQHEL KVQFLWYGTT
810 820 830 840 850
NKRDKSGAYL FLPDGQGQPY VSLRPPFVRV TRGRIYSDVT CFLEHVTHKV
860 870 880 890 900
RLYNIQGIEG QSMEVSNIVN IRNVHNREIV MRISSKINNQ NRYYTDLNGY
910 920 930 940 950
QIQPRRTMSK LPLQANVYPM CTMAYIQDAE HRLTLLSAQS LGASSMASGQ
960 970 980 990 1000
IEVFMDRRLM QDDNRGLGQG VHDNKITANL FRILLEKRSA VNMEEEKKSP
1010 1020 1030 1040 1050
VSYPSLLSHM TSSFLNHPFL PMVLSGQLPS PAFELLSEFP LLQSSLPCDI
1060 1070 1080 1090 1100
HLVNLRTIQS KMGKGYSDEA ALILHRKGFD CQFSSRGIGL PCSTTQGKMS
1110 1120 1130 1140 1150
VLKLFNKFAV ESLVPSSLSL MHSPPDAQNM SEVSLSPMEI STFRIRLRWT
Length:1,150
Mass (Da):131,631
Last modified:July 27, 2011 - v2
Checksum:i5CDD9DD388DF5BC6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti673 – 6731V → G in CAA43480. (PubMed:1757461)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61172 mRNA. Translation: CAA43480.1.
BC138371 mRNA. Translation: AAI38372.1.
BC138372 mRNA. Translation: AAI38373.1.
CCDSiCCDS28938.1.
PIRiA41641.
RefSeqiNP_032575.2. NM_008549.2.
UniGeneiMm.2433.

Genome annotation databases

EnsembliENSMUST00000086723; ENSMUSP00000083928; ENSMUSG00000024085.
GeneIDi17158.
KEGGimmu:17158.
UCSCiuc008dfy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61172 mRNA. Translation: CAA43480.1.
BC138371 mRNA. Translation: AAI38372.1.
BC138372 mRNA. Translation: AAI38373.1.
CCDSiCCDS28938.1.
PIRiA41641.
RefSeqiNP_032575.2. NM_008549.2.
UniGeneiMm.2433.

3D structure databases

ProteinModelPortaliP27046.
SMRiP27046. Positions 127-1019.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP27046. 1 interaction.
MINTiMINT-4996610.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

PTM databases

PhosphoSiteiP27046.

Proteomic databases

MaxQBiP27046.
PaxDbiP27046.
PRIDEiP27046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086723; ENSMUSP00000083928; ENSMUSG00000024085.
GeneIDi17158.
KEGGimmu:17158.
UCSCiuc008dfy.1. mouse.

Organism-specific databases

CTDi4124.
MGIiMGI:104669. Man2a1.

Phylogenomic databases

eggNOGiCOG0383.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000293253.
HOVERGENiHBG052390.
InParanoidiP27046.
KOiK01231.
OMAiFFEHVTH.
OrthoDBiEOG7JQBMJ.
TreeFamiTF313152.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi3.2.1.114. 3474.
ReactomeiREACT_198568. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

ChiTaRSiMan2a1. mouse.
NextBioi291426.
PROiP27046.
SOURCEiSearch...

Gene expression databases

BgeeiP27046.
CleanExiMM_MAN2A1.
ExpressionAtlasiP27046. baseline and differential.
GenevestigatoriP27046.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans."
    Moremen K.W., Robbins P.W.
    J. Cell Biol. 115:1521-1534(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMA2A1_MOUSE
AccessioniPrimary (citable) accession number: P27046
Secondary accession number(s): B9EHS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.