Reviewed, UniProtKB/Swiss-Prot P27036 (CDGT_BACOH)

Names and origin

Protein names

Recommended name:
    Cyclomaltodextrin glucanotransferase
    EC=2.4.1.19
Alternative name(s):
    Cyclodextrin-glycosyltransferase
      Short name=CGTase

Gene names

Name:

cgt

Organism

Bacillus ohbensis

Taxonomic identifier

1481 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Secreted By similarity.
Domain	May consist of two protein domains: the one in the N-terminal side cleaves the alpha-1,4-glucosidic bond in starch, and the other in the C-terminal side catalyzes other activities, including the reconstitution of an alpha-1,4-glucosidic linkage for cyclizing the maltooligosaccharide produced.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. Contains 1 CBM20 (carbohydrate binding type-20) domain. Contains 1 IPT/TIG domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro cyclomaltodextrin glucanotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29
	Chain	30 – 704	675	Cyclomaltodextrin glucanotransferase		PRO_0000001436
Regions
	Domain	520 – 598	79	IPT/TIG
	Domain	599 – 704	106	CBM20
Sites
	Active site	251	1	By similarity
	Active site	279	1	By similarity
	Active site	350	1	By similarity
	Metal binding	52	1	Calcium 2 By similarity
	Metal binding	57	1	Calcium 2 By similarity
	Metal binding	58	1	Calcium 2 By similarity
	Metal binding	76	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	78	1	Calcium 2 By similarity
	Metal binding	161	1	Calcium 1 By similarity
	Metal binding	212	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	221	1	Calcium 1 By similarity
	Metal binding	255	1	Calcium 1; via carbonyl oxygen By similarity
Amino acid modifications
	Disulfide bond	68 ↔ 75		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27036-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 04FA14951D5ACECB	FASTA	704	78,621
	10 20 30 40 50 60 MKNLTVLLKT IPLALLLFIL LSLPTAAQAD VTNKVNYTRD VIYQIVTDRF SDGDPSNNPT 70 80 90 100 110 120 GAIYSQDCSD LHKYCGGDWQ GIIDKINDGY LTDLGITAIW ISQPVENVYA LHPSGYTSYH 130 140 150 160 170 180 GYWARDYKRT NPFYGDFSDF DRLMDTAHSN GIKVIMDFTP NHSSPALETD PSYAENGAVY 190 200 210 220 230 240 NDGVLIGNYS NDPNNLFHHN GGTDFSSYED SIYRNLYDLA DYDLNNTVMD QYLKESIKLW 250 260 270 280 290 300 LDKGIDGIRV DAVKHMSEGW QTSLMSDIYA HEPVFTFGEW FLGSGEVDPQ NHHFANESGM 310 320 330 340 350 360 SLLDFQFGQT IRDVLMDGSS NWYDFNEMIA STEEDYDEVI DQVTFIDNHD MSRFSFEQSS 370 380 390 400 410 420 NRHTDIALAV LLTSRGVPTI YYGTEQYLTG GNDPENRKPM SDFDRTTNSY QIISTLASLR 430 440 450 460 470 480 QNNPALGYGN TSERWINSDV YIYERSFGDS VVLTAVNSGD TSYTINNLNT SLPQGQYTDE 490 500 510 520 530 540 LQQLLDGNEI TVNSNGAVDS FQLSANGVSV WQITEEHASP LIGHVGPMMG KHGNTVTITG 550 560 570 580 590 600 EGFGDNEGSV LFDSDFSDVL SWSDTKIEVS VPDVTAGHYD ISVVNAGDSQ SPTYDKFEVL 610 620 630 640 650 660 TGDQVSIRFA VNNATTSLGT NLYMVGNVNE LGNWDPDQAI GPMFNQVMYQ YPTWYYDISV 670 680 690 700 PAEENLEYKF IKKDSSGNVV WESGNNHTYT TPATGTDTVL VDWQ

References

[1]

"Cloning and sequencing of a cyclodextrin glucanotransferase gene from Bacillus ohbensis and its expression in Escherichia coli." Sin K.A., Nakamura A., Kobayashi K., Masaki H., Uozumi T. Appl. Microbiol. Biotechnol. 35:600-605(1991) [PubMed: 1368710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90243 Genomic DNA. Translation: BAA14289.2.
PIR	I39805.
3D structure databases
HSSP	HSSP built from PDB template 1CYG based on UniProtKB P31797.
ModBase	Search...
Protein family/group databases
CAZy	CBM20. Carbohydrate-Binding Module Family 20. GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BRENDA	2.4.1.19. 264714.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR002044. Glyco_hydro_carb-bd. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR002909. IPT_TIG_rcpt. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF00686. CBM_20. 1 hit. PF01833. TIG. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
ProDom	PD001568. Glyco_hydro_CBD. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
PROSITE	PS51166. CBM20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDGT_BACOH

Accession

Primary (citable) accession number: P27036

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)