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Protein

Endoglucanase CelA

Gene

celA

Organism
Streptomyces lividans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei286Proton donorBy similarity1
Active sitei378NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM2 Carbohydrate-Binding Module Family 2
GH5 Glycoside Hydrolase Family 5

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase CelA (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:celA
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000000787328 – 459Endoglucanase CelAAdd BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 131By similarity

Post-translational modificationi

The linker region (also termed "hinge") may be a potential site for proteolysis.

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP27035

Structurei

3D structure databases

ProteinModelPortaliP27035
SMRiP27035
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 134CBM2PROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 147Linker ("hinge") (Pro-Thr box)Add BLAST12
Regioni148 – 357CatalyticAdd BLAST210

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.290, 1 hit
InterProiView protein in InterPro
IPR001919 CBD2
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR012291 CBM2_carb-bd_dom_sf
IPR018366 CBM2_CS
IPR001547 Glyco_hydro_5
IPR018087 Glyco_hydro_5_CS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00553 CBM_2, 1 hit
PF00150 Cellulase, 1 hit
SMARTiView protein in SMART
SM00637 CBD_II, 1 hit
SUPFAMiSSF49384 SSF49384, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS51173 CBM2, 1 hit
PS00561 CBM2_A, 1 hit
PS00659 GLYCOSYL_HYDROL_F5, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLLALLAT GVSIVGLTAL AGPPAQAATG CKAEYTITSQ WEGGFQAGVK
60 70 80 90 100
ITNLGDPVSG WTLGFTMPDA GQRLVQGWNA TWSQSGSAVT AGGVDWNRTL
110 120 130 140 150
ATGASADLGF VGSFTGANPA PTSFTLNGAT CSGSVTDPPT DPPTDPPATG
160 170 180 190 200
TPAAVNGQLH VCGVHLCNQY DRPIQLRGMS THGIQWFGPC YGDASLDRLA
210 220 230 240 250
QDWKSDLLRV AMYVQEDGYE TDPAGFTSRV NGLVDMAEDR GMYAVIDFHT
260 270 280 290 300
LTPGDPNYNL DRARTFFSSV AARNDKKNVI YEIANEPNGV SWTAVKSYAE
310 320 330 340 350
QVIPVIRAAD PDAVVIVGTR GWSSLGVSDG ANESEVVNNP VNATNIMYAF
360 370 380 390 400
HFYAASHKDD YRAAVRPAAT RLPLFVSEFG TVSATAWSVD RSSSVAWLDL
410 420 430 440 450
LDQLKISYAN WTYSDADEGS AAFRPGTCEG TDYSSSGVLT ESGALVKSRI

STTDDFPTS
Length:459
Mass (Da):48,663
Last modified:December 1, 1992 - v2
Checksum:iCFF47EC34E2F97A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82807 Genomic DNA No translation available.

Similar proteinsi

Entry informationi

Entry nameiGUNA_STRLI
AccessioniPrimary (citable) accession number: P27035
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 1, 1992
Last modified: May 23, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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