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P27033 (GUNC_CELJU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase C

EC=3.2.1.4
Alternative name(s):
Cellodextrinase C
Cellulase C
Endo-1,4-beta-glucanase C
Short name=EGC
Gene names
Name:celC
Synonyms:cel5A
Ordered Locus Names:CJA_1462
OrganismCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) [Complete proteome] [HAMAP]
Taxonomic identifier498211 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio

Protein attributes

Sequence length747 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 1 CBM10 (carbohydrate binding type-10) domain.

Contains 1 CBM2 (carbohydrate binding type-2) domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3737 Ref.1
Chain38 – 747710Endoglucanase C
PRO_0000007865

Regions

Domain38 – 13699CBM2
Domain182 – 20928CBM10
Region280 – 747468Catalytic
Compositional bias137 – 17943Ser-rich (linker)
Compositional bias227 – 27953Ser-rich (linker)

Sites

Active site5021Proton donor By similarity
Active site6521Nucleophile By similarity

Amino acid modifications

Disulfide bond39 ↔ 133 By similarity
Disulfide bond183 ↔ 214 By similarity
Disulfide bond193 ↔ 208 By similarity

Experimental info

Sequence conflict851A → P in CAA43597. Ref.1
Sequence conflict1811G → GG in CAA43597. Ref.1
Sequence conflict2621S → C in CAA43597. Ref.1
Sequence conflict2911Q → K in CAA43597. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P27033 [UniParc].

Last modified April 20, 2010. Version 2.
Checksum: 2A1D90E2612D361A

FASTA74780,098
        10         20         30         40         50         60 
MGHVTSPSKR YPASFKRAGS ILGVSIALAA FSNVAAAGCE YVVTNSWGSG FTAAIRITNS 

        70         80         90        100        110        120 
TSSVINGWNV SWQYNSNRVT NLWNANLSGS NPYSASNLSW NGTIQPGQTV EFGFQGVTNS 

       130        140        150        160        170        180 
GTVESPTVNG AACTGGTSSS VSSSSVVSSS SSSRSSVSSS SVVSSSSSVV SSSSSSVVSG 

       190        200        210        220        230        240 
GQCNWYGTLY PLCVSTTSGW GYENNRSCIS PSTCSAQPAP YGIVGGSSSP SSISSSSVRS 

       250        260        270        280        290        300 
SSSSSVVPPS SSSSSSVPSS SSSSVSSSSV VSSSSSSVSV PGTGVFRVNT QGNLTKDGQL 

       310        320        330        340        350        360 
LPARCGNWFG LEGRHEPSND ADNPSGAPME LYAGNMWWVN NSQGSGRTIQ QTMTELKQQG 

       370        380        390        400        410        420 
ITMLRLPIAP QTLDANDPQG RSPNLKNHQS IRQSNARQAL EDFIKLADQN DIQIFIDIHS 

       430        440        450        460        470        480 
CSNYVGWRAG RLDARPPYVD ANRVGYDFTR EEYSCSATNN PSSVTRIHAY DKQKWLANLR 

       490        500        510        520        530        540 
EIAGLSAKLG VSNLIGIDVF NEPYDYTWAE WKGMVEEAYQ AINEVNPNML IIVEGISANA 

       550        560        570        580        590        600 
NTQDGTPDTS VPVPHGSTDL NPNWGENLYE AGANPPNIPK DRLLFSPHTY GPSVFVQRQF 

       610        620        630        640        650        660 
MDPAQTECAG LEGDEAAQAR CRIVINPTVL EQGWEEHFGY LRELGYGILI GEFGGNMDWP 

       670        680        690        700        710        720 
GAKSSQADRN AWSHITTNVD QQWQQAAASY FKRKGINACY WSMNPESADT MGWYLTPWDP 

       730        740 
VTANDMWGQW TGFDPRKTQL LHNMWGL 

« Hide

References

« Hide 'large scale' references
[1]"The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains."
Ferreira L.M.A., Hazlewood G.P., Barker P.J., Gilbert H.J.
Biochem. J. 279:793-799(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-47.
[2]"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ueda107.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61299 Genomic DNA. Translation: CAA43597.1.
CP000934 Genomic DNA. Translation: ACE82870.1.
PIRS19652.
RefSeqYP_001981949.1. NC_010995.1.

3D structure databases

ProteinModelPortalP27033.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498211.CJA_1462.

Protein family/group databases

CAZyCBM10. Carbohydrate-Binding Module Family 10.
CBM2. Carbohydrate-Binding Module Family 2.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE82870; ACE82870; CJA_1462.
GeneID6413622.
KEGGcja:CJA_1462.
PATRIC21326306. VBICelJap122165_1442.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000066200.
OMARTIQQTM.
OrthoDBEOG6PS5R7.

Enzyme and pathway databases

BioCycCJAP498211:GHIT-1456-MONOMER.

Family and domain databases

Gene3D2.30.32.30. 1 hit.
2.60.40.290. 1 hit.
3.20.20.80. 2 hits.
InterProIPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR002883. CBM10/Dockerin_dom.
IPR018366. CBM2_CS.
IPR009031. CBM_fam10.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02013. CBM_10. 1 hit.
PF00553. CBM_2. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTSM00637. CBD_II. 1 hit.
SM01064. CBM_10. 1 hit.
[Graphical view]
SUPFAMSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 2 hits.
SSF57615. SSF57615. 1 hit.
PROSITEPS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNC_CELJU
AccessionPrimary (citable) accession number: P27033
Secondary accession number(s): B3PDK2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: April 20, 2010
Last modified: May 14, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries