Reviewed, UniProtKB/Swiss-Prot P27033 (GUNC_PSEFL)

Names and origin

Protein names

Recommended name:
    Endoglucanase C
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase C
      Short name=EGC
    Cellulase C
    Cellodextrinase C

Gene names

Name:

celC

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM2 (carbohydrate binding type-2) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Ref.1
	Chain	38 – 748	711	Endoglucanase C		PRO_0000007865
Regions
	Domain	38 – 136	99	CBM2
	Region	281 – 748	468	Catalytic
	Compositional bias	137 – 179	43	Ser-rich (linker)
	Compositional bias	228 – 280	53	Ser-rich (linker)
Sites
	Active site	503	1	Proton donor By similarity
	Active site	653	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	39 ↔ 133		By similarity
	Disulfide bond	184 ↔ 215		By similarity
	Disulfide bond	194 ↔ 209		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27033-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: AC96104137932B76	FASTA	748	80,197
	10 20 30 40 50 60 MGHVTSPSKR YPASFKRAGS ILGVSIALAA FSNVAAAGCE YVVTNSWGSG FTAAIRITNS 70 80 90 100 110 120 TSSVINGWNV SWQYNSNRVT NLWNPNLSGS NPYSASNLSW NGTIQPGQTV EFGFQGVTNS 130 140 150 160 170 180 GTVESPTVNG AACTGGTSSS VSSSSVVSSS SSSRSSVSSS SVVSSSSSVV SSSSSSVVSG 190 200 210 220 230 240 GGQCNWYGTL YPLCVSTTSG WGYENNRSCI SPSTCSAQPA PYGIVGGSSS PSSISSSSVR 250 260 270 280 290 300 SSSSSSVVPP SSSSSSSVPS SSCSSVSSSS VVSSSSSSVS VPGTGVFRVN TKGNLTKDGQ 310 320 330 340 350 360 LLPARCGNWF GLEGRHEPSN DADNPSGAPM ELYAGNMWWV NNSQGSGRTI QQTMTELKQQ 370 380 390 400 410 420 GITMLRLPIA PQTLDANDPQ GRSPNLKNHQ SIRQSNARQA LEDFIKLADQ NDIQIFIDIH 430 440 450 460 470 480 SCSNYVGWRA GRLDARPPYV DANRVGYDFT REEYSCSATN NPSSVTRIHA YDKQKWLANL 490 500 510 520 530 540 REIAGLSAKL GVSNLIGIDV FNEPYDYTWA EWKGMVEEAY QAINEVNPNM LIIVEGISAN 550 560 570 580 590 600 ANTQDGTPDT SVPVPHGSTD LNPNWGENLY EAGANPPNIP KDRLLFSPHT YGPSVFVQRQ 610 620 630 640 650 660 FMDPAQTECA GLEGDEAAQA RCRIVINPTV LEQGWEEHFG YLRELGYGIL IGEFGGNMDW 670 680 690 700 710 720 PGAKSSQADR NAWSHITTNV DQQWQQAAAS YFKRKGINAC YWSMNPESAD TMGWYLTPWD 730 740 PVTANDMWGQ WTGFDPRKTQ LLHNMWGL

References

[1]

"The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains." Ferreira L.M.A., Hazlewood G.P., Barker P.J., Gilbert H.J. Biochem. J. 279:793-799(1991) [PubMed: 1953673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-47. Strain: Sp. Cellulosa.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61299 Genomic DNA. Translation: CAA43597.1.
PIR	S19652.
3D structure databases
HSSP	HSSP built from PDB template 1E8R based on UniProtKB P14768.
ModBase	Search...
Protein family/group databases
CAZy	CBM10. Carbohydrate-Binding Module Family 10. CBM2. Carbohydrate-Binding Module Family 2. GH5. Glycoside Hydrolase Family 5.
Enzyme and pathway databases
BRENDA	3.2.1.4. 329.
Family and domain databases
InterPro	IPR001919. CBD_bac. IPR012291. CBD_carb_bd. IPR018366. CBM2_CS. IPR002883. Dockerin_CBD_5. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR009031. TypeX_cellulose-bd_reg_CBDX. [Graphical view]
Gene3D	G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.30.32.30. TypeX_cellulose-bd_reg_CBDX. 1 hit.
Pfam	PF02013. CBM_10. 1 hit. PF00553. CBM_2. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view]
SMART	SM00637. CBD_II. 1 hit. [Graphical view]
PROSITE	PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNC_PSEFL

Accession

Primary (citable) accession number: P27033

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)