Reviewed,
UniProtKB/Swiss-Prot P27033 (GUNC_PSEFL)
Last modified
June 16, 2009.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Endoglucanase C EC=3.2.1.4 Alternative name(s): Endo-1,4-beta-glucanase C Short name=EGC Cellulase C Cellodextrinase C | ||
| Gene names |
| ||
| Organism | Pseudomonas fluorescens | ||
| Taxonomic identifier | 294 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
Protein attributes
| Sequence length | 748 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. |
| Sequence similarities | Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM2 (carbohydrate binding type-2) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Cellulose degradation Polysaccharide degradation |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | cation binding Inferred from electronic annotation. Source: InterPro cellulase activityInferred from electronic annotation. Source: EC polysaccharide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 37 | 37 | Ref.1 | ||||||||
| Chain | 38 – 748 | 711 | Endoglucanase C | PRO_0000007865 | |||||||
Regions | |||||||||||
| Domain | 38 – 136 | 99 | CBM2 | ||||||||
| Region | 281 – 748 | 468 | Catalytic | ||||||||
| Compositional bias | 137 – 179 | 43 | Ser-rich (linker) | ||||||||
| Compositional bias | 228 – 280 | 53 | Ser-rich (linker) | ||||||||
Sites | |||||||||||
| Active site | 503 | 1 | Proton donor By similarity | ||||||||
| Active site | 653 | 1 | Nucleophile By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 39 ↔ 133 | By similarity | |||||||||
| Disulfide bond | 184 ↔ 215 | By similarity | |||||||||
| Disulfide bond | 194 ↔ 209 | By similarity | |||||||||
Sequences
| ||||||||||||||||||
References
| [1] | "The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains." Ferreira L.M.A., Hazlewood G.P., Barker P.J., Gilbert H.J. Biochem. J. 279:793-799(1991) [PubMed: 1953673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-47. Strain: Sp. Cellulosa. |
Cross-references
Sequence databases | |
|---|---|
| X61299 Genomic DNA. Translation: CAA43597.1. | |
| PIR | S19652. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1E8R based on UniProtKB P14768. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | CBM10. Carbohydrate-Binding Module Family 10. CBM2. Carbohydrate-Binding Module Family 2. GH5. Glycoside Hydrolase Family 5. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.4. 329. |
Family and domain databases | |
| InterPro | IPR001919. CBD_bac. IPR012291. CBD_carb_bd. IPR018366. CBM2_CS. IPR002883. Dockerin_CBD_5. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR009031. TypeX_cellulose-bd_reg_CBDX. [Graphical view] |
| Gene3D | G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.30.32.30. TypeX_cellulose-bd_reg_CBDX. 1 hit. |
| Pfam | PF02013. CBM_10. 1 hit. PF00553. CBM_2. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view] |
| SMART | SM00637. CBD_II. 1 hit. [Graphical view] |
| PROSITE | PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GUNC_PSEFL | ||||||||
| Accession | Primary (citable) accession number: P27033 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


