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P27033

- GUNC_CELJU

UniProt

P27033 - GUNC_CELJU

Protein

Endoglucanase C

Gene

celC

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei502 – 5021Proton donorBy similarity
    Active sitei652 – 6521NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCJAP498211:GHIT-1456-MONOMER.

    Protein family/group databases

    CAZyiCBM10. Carbohydrate-Binding Module Family 10.
    CBM2. Carbohydrate-Binding Module Family 2.
    GH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase C (EC:3.2.1.4)
    Alternative name(s):
    Cellodextrinase C
    Cellulase C
    Endo-1,4-beta-glucanase C
    Short name:
    EGC
    Gene namesi
    Name:celC
    Synonyms:cel5A
    Ordered Locus Names:CJA_1462
    OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
    Taxonomic identifieri498211 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
    ProteomesiUP000001036: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 37371 PublicationAdd
    BLAST
    Chaini38 – 747710Endoglucanase CPRO_0000007865Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi39 ↔ 133By similarity
    Disulfide bondi183 ↔ 214By similarity
    Disulfide bondi193 ↔ 208By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi498211.CJA_1462.

    Structurei

    3D structure databases

    ProteinModelPortaliP27033.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 13699CBM2Add
    BLAST
    Domaini182 – 20928CBM10Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni280 – 747468CatalyticAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi137 – 17943Ser-rich (linker)Add
    BLAST
    Compositional biasi227 – 27953Ser-rich (linker)Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000066200.
    OMAiRTIQQTM.
    OrthoDBiEOG6PS5R7.

    Family and domain databases

    Gene3Di2.30.32.30. 1 hit.
    2.60.40.290. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR002883. CBM10/Dockerin_dom.
    IPR018366. CBM2_CS.
    IPR009031. CBM_fam10.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02013. CBM_10. 1 hit.
    PF00553. CBM_2. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view]
    SMARTiSM00637. CBD_II. 1 hit.
    SM01064. CBM_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF49384. SSF49384. 1 hit.
    SSF51445. SSF51445. 2 hits.
    SSF57615. SSF57615. 1 hit.
    PROSITEiPS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27033-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGHVTSPSKR YPASFKRAGS ILGVSIALAA FSNVAAAGCE YVVTNSWGSG    50
    FTAAIRITNS TSSVINGWNV SWQYNSNRVT NLWNANLSGS NPYSASNLSW 100
    NGTIQPGQTV EFGFQGVTNS GTVESPTVNG AACTGGTSSS VSSSSVVSSS 150
    SSSRSSVSSS SVVSSSSSVV SSSSSSVVSG GQCNWYGTLY PLCVSTTSGW 200
    GYENNRSCIS PSTCSAQPAP YGIVGGSSSP SSISSSSVRS SSSSSVVPPS 250
    SSSSSSVPSS SSSSVSSSSV VSSSSSSVSV PGTGVFRVNT QGNLTKDGQL 300
    LPARCGNWFG LEGRHEPSND ADNPSGAPME LYAGNMWWVN NSQGSGRTIQ 350
    QTMTELKQQG ITMLRLPIAP QTLDANDPQG RSPNLKNHQS IRQSNARQAL 400
    EDFIKLADQN DIQIFIDIHS CSNYVGWRAG RLDARPPYVD ANRVGYDFTR 450
    EEYSCSATNN PSSVTRIHAY DKQKWLANLR EIAGLSAKLG VSNLIGIDVF 500
    NEPYDYTWAE WKGMVEEAYQ AINEVNPNML IIVEGISANA NTQDGTPDTS 550
    VPVPHGSTDL NPNWGENLYE AGANPPNIPK DRLLFSPHTY GPSVFVQRQF 600
    MDPAQTECAG LEGDEAAQAR CRIVINPTVL EQGWEEHFGY LRELGYGILI 650
    GEFGGNMDWP GAKSSQADRN AWSHITTNVD QQWQQAAASY FKRKGINACY 700
    WSMNPESADT MGWYLTPWDP VTANDMWGQW TGFDPRKTQL LHNMWGL 747
    Length:747
    Mass (Da):80,098
    Last modified:April 20, 2010 - v2
    Checksum:i2A1D90E2612D361A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851A → P in CAA43597. (PubMed:1953673)Curated
    Sequence conflicti181 – 1811G → GG in CAA43597. (PubMed:1953673)Curated
    Sequence conflicti262 – 2621S → C in CAA43597. (PubMed:1953673)Curated
    Sequence conflicti291 – 2911Q → K in CAA43597. (PubMed:1953673)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61299 Genomic DNA. Translation: CAA43597.1.
    CP000934 Genomic DNA. Translation: ACE82870.1.
    PIRiS19652.
    RefSeqiYP_001981949.1. NC_010995.1.

    Genome annotation databases

    EnsemblBacteriaiACE82870; ACE82870; CJA_1462.
    GeneIDi6413622.
    KEGGicja:CJA_1462.
    PATRICi21326306. VBICelJap122165_1442.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61299 Genomic DNA. Translation: CAA43597.1 .
    CP000934 Genomic DNA. Translation: ACE82870.1 .
    PIRi S19652.
    RefSeqi YP_001981949.1. NC_010995.1.

    3D structure databases

    ProteinModelPortali P27033.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 498211.CJA_1462.

    Protein family/group databases

    CAZyi CBM10. Carbohydrate-Binding Module Family 10.
    CBM2. Carbohydrate-Binding Module Family 2.
    GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACE82870 ; ACE82870 ; CJA_1462 .
    GeneIDi 6413622.
    KEGGi cja:CJA_1462.
    PATRICi 21326306. VBICelJap122165_1442.

    Phylogenomic databases

    HOGENOMi HOG000066200.
    OMAi RTIQQTM.
    OrthoDBi EOG6PS5R7.

    Enzyme and pathway databases

    BioCyci CJAP498211:GHIT-1456-MONOMER.

    Family and domain databases

    Gene3Di 2.30.32.30. 1 hit.
    2.60.40.290. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR002883. CBM10/Dockerin_dom.
    IPR018366. CBM2_CS.
    IPR009031. CBM_fam10.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02013. CBM_10. 1 hit.
    PF00553. CBM_2. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SMARTi SM00637. CBD_II. 1 hit.
    SM01064. CBM_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49384. SSF49384. 1 hit.
    SSF51445. SSF51445. 2 hits.
    SSF57615. SSF57615. 1 hit.
    PROSITEi PS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains."
      Ferreira L.M.A., Hazlewood G.P., Barker P.J., Gilbert H.J.
      Biochem. J. 279:793-799(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-47.
    2. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
      DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
      J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ueda107.

    Entry informationi

    Entry nameiGUNC_CELJU
    AccessioniPrimary (citable) accession number: P27033
    Secondary accession number(s): B3PDK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3