P27032 (GUNY_DICD3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Minor endoglucanase Y
EC=3.2.1.4

Alternative name(s):
Cellulase Y
Endo-1,4-beta-glucanase Y
Short name=EGY

Gene names

Name:	celY
Ordered Locus Names:	Dda3937_01994

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) [Complete proteome] [HAMAP]

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya › Dickeya dadantii

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represents only 3% of the global cellulase activity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 8 (cellulase D) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 332	309	Minor endoglucanase Y		PRO_0000007937
Sites
	Active site	53	1	Proton donor By similarity
	Active site	110	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	P27032 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 6EA760A2B8227079	FASTA	332	37,593
	10 20 30 40 50 60 MGKPMWRCWA LMLMVWFSAS ATAANGWEIY KSRFMTTDGR IQDTGNKNVS HTEGQGFAML 70 80 90 100 110 120 MAVHYDDRIA FDNLWNWTQS HLRNTTSGLF YWRYDPSAAN PVVDKNNASD GDVLIAWALL 130 140 150 160 170 180 KAGNKWQDNR YLQASDSIQK AIIASNIIQF AGRTVMLPGA YGFNKNSYVI LNPSYFLFPA 190 200 210 220 230 240 WRDFANRSHL QVWRQLIDDS LSLVGEMRFG QVGLPTDWAA LNADGSMAPA TAWPSRFSYD 250 260 270 280 290 300 AIRIPLYLYW YDAKTTALVP FQLYWRNYPR LTTPAWVDVL SSNTATYNMQ GGLLAVRDLT 310 320 330 MGNLDGLSDL PGASEDYYSS SLRLLVMLAR GK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the cellulase-encoding celY gene of Erwinia chrysanthemi: a possible case of interspecies gene transfer." Guiseppi A., Aymeric J.-L., Cami B., Barras F., Creuzet N. Gene 106:109-114(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-33. Strain: 3937.
[2]	"Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937." Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N., Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D., Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K., Rauscher L., Rodriguez-Palenzuela P., Toussaint A. , Holeva M.C., He S.Y., Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S., Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S., Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M., Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R., Blattner F.R., Keen N.T., Perna N.T. J. Bacteriol. 193:2076-2077(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74044 Genomic DNA. Translation: AAA24818.1. CP002038 Genomic DNA. Translation: ADM96331.1.
PIR	JT0585.
RefSeq	YP_003880888.1. NC_014500.1.
3D structure databases
ProteinModelPortal	P27032.
ModBase	Search...
Protein family/group databases
CAZy	GH8. Glycoside Hydrolase Family 8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADM96331; ADM96331; Dda3937_01994.
GeneID	9731500.
KEGG	ddd:Dda3937_01994.
PATRIC	42312535. VBIDicDad25310_0121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000250877.
KO	K01179.
OMA	VSHTEGQ.
Enzyme and pathway databases
BioCyc	DDAD198628:GHFQ-114-MONOMER.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR002037. Glyco_hydro_8. IPR019834. Glyco_hydro_8_CS. [Graphical view]
Pfam	PF01270. Glyco_hydro_8. 1 hit. [Graphical view]
PRINTS	PR00735. GLHYDRLASE8.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00812. GLYCOSYL_HYDROL_F8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNY_DICD3

Accession

Primary (citable) accession number: P27032
Secondary accession number(s): E0SES8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program