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P27028

- PPCE_ELIME

UniProt

P27028 - PPCE_ELIME

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Protein

Prolyl endopeptidase

Gene

f1pep1

Organism
Elizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has an absolute requirement for an X-Pro bond in the trans configuration immediately preceding the Pro-Y scissible bond.

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei556 – 5561Charge relay system1 PublicationPROSITE-ProRule annotation
Active sitei675 – 6751Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type exopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl endopeptidase (EC:3.4.21.26)
Alternative name(s):
Post-proline cleaving enzyme
Proline-specific endopeptidase
Short name:
PE
Short name:
PSE
Gene namesi
Name:f1pep1
OrganismiElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Taxonomic identifieri238 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 705685Prolyl endopeptidasePRO_0000027207Add
BLAST

Proteomic databases

PRIDEiP27028.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP27028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27028-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKYNKLSVAV AAFAFAAVSA QNSNVLKYPE TKKVSHTDTY FGTQVSDPYR
60 70 80 90 100
WLEDDRAEDT KAWVQQEVKF TQDYLAQIPF RDQLKKQLMD IWNYEKISAP
110 120 130 140 150
FKKGKYTYFS KNDGLQAQSV LYRKDAAGKT EVFLDPNKFS EKGTTSLASV
160 170 180 190 200
SFNKKGTLVA YSISEGGSDW NKIIILDAET KKQLDETLLD VKFSGISWLG
210 220 230 240 250
DEGFFYSSYD KPKEGSVLSG MTDKHKVYFH KLGTKQSQDE LIIGGDKFPR
260 270 280 290 300
RYIGAYVTDD QRYLVVSAAN ATNGNELYIK DLKNKTDFIP IITGFDSNVN
310 320 330 340 350
VADTDGDTLY LFTDKDAPNK RLVKTTIQNP KAETWKDVIA ETSEPLEINT
360 370 380 390 400
GGGYFFATYM KDAIDQVKQY DKNGKLVRAI KLPGSGNASG FGGEKTEKDL
410 420 430 440 450
YYSFTNYITP PTIFKYNVTT GNSEVYQKPK VKFNPENYVS EQVFYTSSDG
460 470 480 490 500
TKIPMMISYK KGLKKDGKNP TILYSYGGFN ISLQPAFSVV NAIWMENGGI
510 520 530 540 550
YAVPNIRGGG EYGKKWHDAG TKMQKKNVFN DFIAAGEYLQ KNGYTSKEYM
560 570 580 590 600
ALSGRSNGGL LVGATMTMRP DLAKVAFPGV GVLDMLRYNK FTAGAGWAYD
610 620 630 640 650
YGTAEDSKEM FEYLKSYSPV HNVKAGTCYP STMVITSDHD DRVVPAHSFK
660 670 680 690 700
FGSELQAKQS CKNPILIRIE TNAGHGAGRS TEQVVAENAD LLSFALYEMG

IKSLK
Length:705
Mass (Da):78,707
Last modified:August 1, 1992 - v1
Checksum:iBC0EDCBABB328256
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101S → C AA sequence (PubMed:7764331)Curated
Sequence conflicti587 – 5871R → A AA sequence (PubMed:7764331)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10980 Genomic DNA. Translation: BAA01755.1.
X63674 Genomic DNA. Translation: CAA45213.1.
PIRiJX0194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10980 Genomic DNA. Translation: BAA01755.1 .
X63674 Genomic DNA. Translation: CAA45213.1 .
PIRi JX0194.

3D structure databases

ProteinModelPortali P27028.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P27028.
ChEMBLi CHEMBL6043.

Proteomic databases

PRIDEi P27028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view ]
PANTHERi PTHR11757. PTHR11757. 1 hit.
Pfami PF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view ]
PRINTSi PR00862. PROLIGOPTASE.
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prolyl endopeptidase from Flavobacterium meningosepticum: cloning and sequencing of the enzyme gene."
    Yoshimoto T., Kanatani A., Shimoda T., Inaoka T., Kokubo T., Tsuru D.
    J. Biochem. 110:873-878(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Cloning of proline-specific endopeptidase gene from Flavobacterium meningosepticum: expression in Escherichia coli and purification of the heterologous protein."
    Diefenthal T., Dargatz H., Witte V., Reipen G., Svendsen I.
    Appl. Microbiol. Biotechnol. 40:90-97(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiPPCE_ELIME
AccessioniPrimary (citable) accession number: P27028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3