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P27028 (PPCE_ELIME) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prolyl endopeptidase

EC=3.4.21.26
Alternative name(s):
Post-proline cleaving enzyme
Proline-specific endopeptidase
Short name=PE
Short name=PSE
Gene names
Name:f1pep1
OrganismElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Taxonomic identifier238 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has an absolute requirement for an X-Pro bond in the trans configuration immediately preceding the Pro-Y scissible bond.

Catalytic activity

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Subunit structure

Monomer.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the peptidase S9A family.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

serine-type exopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 705685Prolyl endopeptidase
PRO_0000027207

Sites

Active site5561Charge relay system Ref.1
Active site6751Charge relay system By similarity

Experimental info

Sequence conflict1101S → C AA sequence Ref.2
Sequence conflict5871R → A AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P27028 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: BC0EDCBABB328256

FASTA70578,707
        10         20         30         40         50         60 
MKYNKLSVAV AAFAFAAVSA QNSNVLKYPE TKKVSHTDTY FGTQVSDPYR WLEDDRAEDT 

        70         80         90        100        110        120 
KAWVQQEVKF TQDYLAQIPF RDQLKKQLMD IWNYEKISAP FKKGKYTYFS KNDGLQAQSV 

       130        140        150        160        170        180 
LYRKDAAGKT EVFLDPNKFS EKGTTSLASV SFNKKGTLVA YSISEGGSDW NKIIILDAET 

       190        200        210        220        230        240 
KKQLDETLLD VKFSGISWLG DEGFFYSSYD KPKEGSVLSG MTDKHKVYFH KLGTKQSQDE 

       250        260        270        280        290        300 
LIIGGDKFPR RYIGAYVTDD QRYLVVSAAN ATNGNELYIK DLKNKTDFIP IITGFDSNVN 

       310        320        330        340        350        360 
VADTDGDTLY LFTDKDAPNK RLVKTTIQNP KAETWKDVIA ETSEPLEINT GGGYFFATYM 

       370        380        390        400        410        420 
KDAIDQVKQY DKNGKLVRAI KLPGSGNASG FGGEKTEKDL YYSFTNYITP PTIFKYNVTT 

       430        440        450        460        470        480 
GNSEVYQKPK VKFNPENYVS EQVFYTSSDG TKIPMMISYK KGLKKDGKNP TILYSYGGFN 

       490        500        510        520        530        540 
ISLQPAFSVV NAIWMENGGI YAVPNIRGGG EYGKKWHDAG TKMQKKNVFN DFIAAGEYLQ 

       550        560        570        580        590        600 
KNGYTSKEYM ALSGRSNGGL LVGATMTMRP DLAKVAFPGV GVLDMLRYNK FTAGAGWAYD 

       610        620        630        640        650        660 
YGTAEDSKEM FEYLKSYSPV HNVKAGTCYP STMVITSDHD DRVVPAHSFK FGSELQAKQS 

       670        680        690        700 
CKNPILIRIE TNAGHGAGRS TEQVVAENAD LLSFALYEMG IKSLK 

« Hide

References

[1]"Prolyl endopeptidase from Flavobacterium meningosepticum: cloning and sequencing of the enzyme gene."
Yoshimoto T., Kanatani A., Shimoda T., Inaoka T., Kokubo T., Tsuru D.
J. Biochem. 110:873-878(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Cloning of proline-specific endopeptidase gene from Flavobacterium meningosepticum: expression in Escherichia coli and purification of the heterologous protein."
Diefenthal T., Dargatz H., Witte V., Reipen G., Svendsen I.
Appl. Microbiol. Biotechnol. 40:90-97(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10980 Genomic DNA. Translation: BAA01755.1.
X63674 Genomic DNA. Translation: CAA45213.1.
PIRJX0194.

3D structure databases

ProteinModelPortalP27028.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP27028.
ChEMBLCHEMBL6043.

Proteomic databases

PRIDEP27028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.130.10.120. 1 hit.
InterProIPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERPTHR11757. PTHR11757. 1 hit.
PfamPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSPR00862. PROLIGOPTASE.
PROSITEPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPCE_ELIME
AccessionPrimary (citable) accession number: P27028
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: December 11, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries