P27027 (PELD_PSEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectin lyase
EC=4.2.2.10

Gene names

Name:

pnl

Organism

Pseudomonas marginalis

Taxonomic identifier

298 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Induction	By DNA-damaging agents such as mitomycin C.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	pectin lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 312	311	Pectin lyase		PRO_0000212996
Sites
	Active site	201	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P27027 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0A5CFF5610A09D45	FASTA	312	34,103
	10 20 30 40 50 60 MSYPESKLTG LTGFALAAKV TGGWAGPVVS ITNLDQLKAN IGTVTPQVLV INSNISASSL 70 80 90 100 110 120 TKVNMGANKT LIGSFQNRTL ENIHLRATAQ SQNIILQNLI FKHSANIKAN DDIQVYLNYG 130 140 150 160 170 180 SKYWIDHCSF VGHSWSTTDG SEDKLLYIGE KADYATISNC FFGSHKYGLI FGHPADDNNA 190 200 210 220 230 240 AFNGYPRLTL CHNRFDNMEV RAPGLMRYGY FHVYNNYINK FHLGFTLAQN ANILSESNYF 250 260 270 280 290 300 GEGSQNNGML DDKGSGTFTD TNSVPPITNQ KSPKAQWTAT SNYAYTLKTA AQAKDFTQKN 310 AGAQAAALVF GS

References

[1]	"Molecular cloning and nucleotide sequence of a pectin lyase gene from Pseudomonas marginalis N6301." Nikaidou N., Kamio Y., Izaki K. Biochem. Biophys. Res. Commun. 182:14-19(1992) [PubMed: 1731776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-34. Strain: N6301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D32121 Genomic DNA. Translation: BAA06847.1.
PIR	JQ1318.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PELD_PSEMA

Accession

Primary (citable) accession number: P27027

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 31, 2011
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program