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Protein

Aliphatic amidase expression-regulating protein

Gene

amiC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity.

GO - Molecular functioni

  • amide binding Source: PseudoCAP
  • kinase activity Source: UniProtKB-KW

GO - Biological processi

  • amino acid transport Source: InterPro
  • negative regulation of hydrolase activity Source: PseudoCAP
  • regulation of cellular amide catabolic process Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Repressor, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Aliphatic amidase expression-regulating protein
Gene namesi
Name:amiC
Ordered Locus Names:PA3364
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3364.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 385384Aliphatic amidase expression-regulating proteinPRO_0000064581Add
BLAST

Proteomic databases

PaxDbiP27017.

Interactioni

Subunit structurei

Homodimer. Forms a complex with AmiR.

Protein-protein interaction databases

IntActiP27017. 1 interaction.
STRINGi208964.PA3364.

Structurei

Secondary structure

1
385
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 135Combined sources
Beta strandi16 – 183Combined sources
Helixi21 – 3919Combined sources
Turni40 – 434Combined sources
Beta strandi50 – 545Combined sources
Helixi60 – 7213Combined sources
Beta strandi78 – 814Combined sources
Helixi85 – 9713Combined sources
Beta strandi101 – 1044Combined sources
Beta strandi117 – 1193Combined sources
Helixi124 – 1263Combined sources
Helixi128 – 1369Combined sources
Turni137 – 1393Combined sources
Beta strandi141 – 15010Combined sources
Helixi151 – 16616Combined sources
Beta strandi170 – 1778Combined sources
Helixi183 – 19614Combined sources
Beta strandi199 – 2046Combined sources
Helixi209 – 22113Combined sources
Beta strandi229 – 2335Combined sources
Helixi236 – 2394Combined sources
Helixi244 – 2474Combined sources
Beta strandi251 – 2555Combined sources
Helixi263 – 27311Combined sources
Helixi284 – 30320Combined sources
Helixi308 – 3158Combined sources
Beta strandi320 – 3223Combined sources
Beta strandi325 – 3295Combined sources
Turni331 – 3333Combined sources
Beta strandi336 – 3383Combined sources
Beta strandi341 – 3455Combined sources
Beta strandi351 – 3566Combined sources
Helixi369 – 3713Combined sources
Helixi372 – 3787Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PEAX-ray2.10A1-385[»]
1QNLX-ray2.70A1-375[»]
1QO0X-ray2.25A/B1-380[»]
ProteinModelPortaliP27017.
SMRiP27017. Positions 7-380.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27017.

Family & Domainsi

Domaini

Consists of two beta-alpha-beta domains with a central cleft in which the amide binds.

Phylogenomic databases

eggNOGiENOG4107TSX. Bacteria.
ENOG410Y0Z1. LUCA.
HOGENOMiHOG000202643.
InParanoidiP27017.
KOiK01999.
OMAiYPYESNR.
PhylomeDBiP27017.

Family and domain databases

InterProiIPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27017-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSHQERPLI GLLFSETGVT ADIERSQRYG ALLAVEQLNR EGGVGGRPIE
60 70 80 90 100
TLSQDPGGDP DRYRLCAEDF IRNRGVRFLV GCYMSHTRKA VMPVVERADA
110 120 130 140 150
LLCYPTPYEG FEYSPNIVYG GPAPNQNSAP LAAYLIRHYG ERVVFIGSDY
160 170 180 190 200
IYPRESNHVM RHLYRQHGGT VLEEIYIPLY PSDDDVQRAV ERIYQARADV
210 220 230 240 250
VFSTVVGTGT AELYRAIARR YGDGRRPPIA SLTTSEAEVA KMESDVAEGQ
260 270 280 290 300
VVVAPYFSSI DTAASRAFVQ ACHGFFPENA TITAWAEAAY WQTLLLGRAA
310 320 330 340 350
QAAGSWRVED VQRHLYDICI DAPQGPVRVE RQNNHSRLSS RIAEIDARGV
360 370 380
FQVRWQSPEP IRPDPYVVVH NLDDWSASMG GGALP
Length:385
Mass (Da):42,807
Last modified:January 23, 2007 - v5
Checksum:i33924B6C36017B79
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 282QR → HA in CAA32024 (PubMed:1907262).Curated
Sequence conflicti186 – 1861V → L in CAA32024 (PubMed:1907262).Curated
Sequence conflicti263 – 2631A → P in CAA32024 (PubMed:1907262).Curated
Sequence conflicti305 – 3051S → N in CAA32024 (PubMed:1907262).Curated
Sequence conflicti319 – 3191C → D in CAA32024 (PubMed:1907262).Curated
Sequence conflicti383 – 3831A → P in CAA32024 (PubMed:1907262).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti106 – 1061T → N in strain: PAC181; butyramide inducible phenotype.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13776 Genomic DNA. Translation: CAA32024.1.
AE004091 Genomic DNA. Translation: AAG06752.1.
PIRiA40359.
C83226.
RefSeqiNP_252054.1. NC_002516.2.
WP_003091751.1. NZ_ASJY01000541.1.

Genome annotation databases

EnsemblBacteriaiAAG06752; AAG06752; PA3364.
GeneIDi877798.
KEGGipae:PA3364.
PATRICi19841329. VBIPseAer58763_3523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13776 Genomic DNA. Translation: CAA32024.1.
AE004091 Genomic DNA. Translation: AAG06752.1.
PIRiA40359.
C83226.
RefSeqiNP_252054.1. NC_002516.2.
WP_003091751.1. NZ_ASJY01000541.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PEAX-ray2.10A1-385[»]
1QNLX-ray2.70A1-375[»]
1QO0X-ray2.25A/B1-380[»]
ProteinModelPortaliP27017.
SMRiP27017. Positions 7-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP27017. 1 interaction.
STRINGi208964.PA3364.

Proteomic databases

PaxDbiP27017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06752; AAG06752; PA3364.
GeneIDi877798.
KEGGipae:PA3364.
PATRICi19841329. VBIPseAer58763_3523.

Organism-specific databases

PseudoCAPiPA3364.

Phylogenomic databases

eggNOGiENOG4107TSX. Bacteria.
ENOG410Y0Z1. LUCA.
HOGENOMiHOG000202643.
InParanoidiP27017.
KOiK01999.
OMAiYPYESNR.
PhylomeDBiP27017.

Miscellaneous databases

EvolutionaryTraceiP27017.

Family and domain databases

InterProiIPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMIC_PSEAE
AccessioniPrimary (citable) accession number: P27017
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 107 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.