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Protein

Aliphatic amidase expression-regulating protein

Gene

amiC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity.

GO - Molecular functioni

  • amide binding Source: PseudoCAP
  • kinase activity Source: UniProtKB-KW

GO - Biological processi

  • amino acid transport Source: InterPro
  • negative regulation of hydrolase activity Source: PseudoCAP
  • regulation of cellular amide catabolic process Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Repressor, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Aliphatic amidase expression-regulating protein
Gene namesi
Name:amiC
Ordered Locus Names:PA3364
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3364.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000645812 – 385Aliphatic amidase expression-regulating proteinAdd BLAST384

Proteomic databases

PaxDbiP27017.

Interactioni

Subunit structurei

Homodimer. Forms a complex with AmiR.

Protein-protein interaction databases

IntActiP27017. 1 interactor.
STRINGi208964.PA3364.

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 13Combined sources5
Beta strandi16 – 18Combined sources3
Helixi21 – 39Combined sources19
Turni40 – 43Combined sources4
Beta strandi50 – 54Combined sources5
Helixi60 – 72Combined sources13
Beta strandi78 – 81Combined sources4
Helixi85 – 97Combined sources13
Beta strandi101 – 104Combined sources4
Beta strandi117 – 119Combined sources3
Helixi124 – 126Combined sources3
Helixi128 – 136Combined sources9
Turni137 – 139Combined sources3
Beta strandi141 – 150Combined sources10
Helixi151 – 166Combined sources16
Beta strandi170 – 177Combined sources8
Helixi183 – 196Combined sources14
Beta strandi199 – 204Combined sources6
Helixi209 – 221Combined sources13
Beta strandi229 – 233Combined sources5
Helixi236 – 239Combined sources4
Helixi244 – 247Combined sources4
Beta strandi251 – 255Combined sources5
Helixi263 – 273Combined sources11
Helixi284 – 303Combined sources20
Helixi308 – 315Combined sources8
Beta strandi320 – 322Combined sources3
Beta strandi325 – 329Combined sources5
Turni331 – 333Combined sources3
Beta strandi336 – 338Combined sources3
Beta strandi341 – 345Combined sources5
Beta strandi351 – 356Combined sources6
Helixi369 – 371Combined sources3
Helixi372 – 378Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PEAX-ray2.10A1-385[»]
1QNLX-ray2.70A1-375[»]
1QO0X-ray2.25A/B1-380[»]
ProteinModelPortaliP27017.
SMRiP27017.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27017.

Family & Domainsi

Domaini

Consists of two beta-alpha-beta domains with a central cleft in which the amide binds.

Phylogenomic databases

eggNOGiENOG4107TSX. Bacteria.
ENOG410Y0Z1. LUCA.
HOGENOMiHOG000202643.
InParanoidiP27017.
KOiK01999.
OMAiYPYESNR.
PhylomeDBiP27017.

Family and domain databases

InterProiIPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27017-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSHQERPLI GLLFSETGVT ADIERSQRYG ALLAVEQLNR EGGVGGRPIE
60 70 80 90 100
TLSQDPGGDP DRYRLCAEDF IRNRGVRFLV GCYMSHTRKA VMPVVERADA
110 120 130 140 150
LLCYPTPYEG FEYSPNIVYG GPAPNQNSAP LAAYLIRHYG ERVVFIGSDY
160 170 180 190 200
IYPRESNHVM RHLYRQHGGT VLEEIYIPLY PSDDDVQRAV ERIYQARADV
210 220 230 240 250
VFSTVVGTGT AELYRAIARR YGDGRRPPIA SLTTSEAEVA KMESDVAEGQ
260 270 280 290 300
VVVAPYFSSI DTAASRAFVQ ACHGFFPENA TITAWAEAAY WQTLLLGRAA
310 320 330 340 350
QAAGSWRVED VQRHLYDICI DAPQGPVRVE RQNNHSRLSS RIAEIDARGV
360 370 380
FQVRWQSPEP IRPDPYVVVH NLDDWSASMG GGALP
Length:385
Mass (Da):42,807
Last modified:January 23, 2007 - v5
Checksum:i33924B6C36017B79
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27 – 28QR → HA in CAA32024 (PubMed:1907262).Curated2
Sequence conflicti186V → L in CAA32024 (PubMed:1907262).Curated1
Sequence conflicti263A → P in CAA32024 (PubMed:1907262).Curated1
Sequence conflicti305S → N in CAA32024 (PubMed:1907262).Curated1
Sequence conflicti319C → D in CAA32024 (PubMed:1907262).Curated1
Sequence conflicti383A → P in CAA32024 (PubMed:1907262).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti106T → N in strain: PAC181; butyramide inducible phenotype. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13776 Genomic DNA. Translation: CAA32024.1.
AE004091 Genomic DNA. Translation: AAG06752.1.
PIRiA40359.
C83226.
RefSeqiNP_252054.1. NC_002516.2.
WP_003091751.1. NZ_ASJY01000541.1.

Genome annotation databases

EnsemblBacteriaiAAG06752; AAG06752; PA3364.
GeneIDi877798.
KEGGipae:PA3364.
PATRICi19841329. VBIPseAer58763_3523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13776 Genomic DNA. Translation: CAA32024.1.
AE004091 Genomic DNA. Translation: AAG06752.1.
PIRiA40359.
C83226.
RefSeqiNP_252054.1. NC_002516.2.
WP_003091751.1. NZ_ASJY01000541.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PEAX-ray2.10A1-385[»]
1QNLX-ray2.70A1-375[»]
1QO0X-ray2.25A/B1-380[»]
ProteinModelPortaliP27017.
SMRiP27017.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP27017. 1 interactor.
STRINGi208964.PA3364.

Proteomic databases

PaxDbiP27017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06752; AAG06752; PA3364.
GeneIDi877798.
KEGGipae:PA3364.
PATRICi19841329. VBIPseAer58763_3523.

Organism-specific databases

PseudoCAPiPA3364.

Phylogenomic databases

eggNOGiENOG4107TSX. Bacteria.
ENOG410Y0Z1. LUCA.
HOGENOMiHOG000202643.
InParanoidiP27017.
KOiK01999.
OMAiYPYESNR.
PhylomeDBiP27017.

Miscellaneous databases

EvolutionaryTraceiP27017.

Family and domain databases

InterProiIPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMIC_PSEAE
AccessioniPrimary (citable) accession number: P27017
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 109 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.