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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Thermus thermophilus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Cofactori

Mg2+Note: Binds 2 magnesium ions per tetramer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi452Magnesium1
Metal bindingi458Magnesium; via carbonyl oxygen1
Metal bindingi461Magnesium1
Metal bindingi462Magnesium1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:pheT
OrganismiThermus thermophilus
Taxonomic identifieri274 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001269781 – 785Phenylalanine--tRNA ligase beta subunitAdd BLAST785

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Protein-protein interaction databases

DIPiDIP-6105N.
MINTiMINT-88295.
STRINGi262724.TTC1595.

Structurei

Secondary structure

1785
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Helixi5 – 8Combined sources4
Turni9 – 11Combined sources3
Helixi18 – 28Combined sources11
Beta strandi34 – 37Combined sources4
Beta strandi45 – 55Combined sources11
Turni57 – 60Combined sources4
Beta strandi62 – 76Combined sources15
Beta strandi86 – 90Combined sources5
Turni97 – 99Combined sources3
Beta strandi100 – 102Combined sources3
Beta strandi105 – 108Combined sources4
Beta strandi109 – 113Combined sources5
Beta strandi115 – 117Combined sources3
Helixi120 – 123Combined sources4
Beta strandi125 – 127Combined sources3
Helixi146 – 148Combined sources3
Beta strandi154 – 157Combined sources4
Helixi165 – 168Combined sources4
Helixi170 – 179Combined sources10
Beta strandi199 – 205Combined sources7
Turni207 – 209Combined sources3
Beta strandi211 – 220Combined sources10
Helixi228 – 236Combined sources9
Helixi244 – 255Combined sources12
Beta strandi261 – 264Combined sources4
Helixi265 – 267Combined sources3
Beta strandi271 – 276Combined sources6
Beta strandi282 – 285Combined sources4
Beta strandi290 – 292Combined sources3
Beta strandi298 – 304Combined sources7
Beta strandi307 – 313Combined sources7
Turni314 – 316Combined sources3
Beta strandi317 – 319Combined sources3
Turni320 – 322Combined sources3
Beta strandi330 – 337Combined sources8
Helixi340 – 349Combined sources10
Helixi355 – 362Combined sources8
Helixi369 – 383Combined sources15
Beta strandi387 – 390Combined sources4
Beta strandi393 – 395Combined sources3
Beta strandi404 – 406Combined sources3
Helixi408 – 415Combined sources8
Helixi421 – 430Combined sources10
Beta strandi434 – 445Combined sources12
Helixi456 – 467Combined sources12
Helixi469 – 471Combined sources3
Helixi483 – 485Combined sources3
Turni486 – 489Combined sources4
Helixi490 – 505Combined sources16
Beta strandi514 – 516Combined sources3
Helixi520 – 523Combined sources4
Beta strandi532 – 535Combined sources4
Beta strandi541 – 544Combined sources4
Helixi549 – 562Combined sources14
Beta strandi568 – 592Combined sources25
Beta strandi597 – 600Combined sources4
Helixi606 – 620Combined sources15
Beta strandi625 – 627Combined sources3
Beta strandi634 – 644Combined sources11
Beta strandi647 – 655Combined sources9
Helixi657 – 662Combined sources6
Beta strandi669 – 676Combined sources8
Beta strandi693 – 697Combined sources5
Beta strandi701 – 705Combined sources5
Helixi707 – 718Combined sources12
Beta strandi722 – 729Combined sources8
Beta strandi733 – 736Combined sources4
Helixi737 – 739Combined sources3
Beta strandi744 – 749Combined sources6
Beta strandi752 – 754Combined sources3
Helixi758 – 773Combined sources16
Turni774 – 776Combined sources3
Beta strandi782 – 784Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B70X-ray2.70B1-785[»]
1B7YX-ray2.50B1-785[»]
2AKWX-ray2.80B1-785[»]
2ALYX-ray2.60B1-785[»]
2AMCX-ray2.70B1-785[»]
2IY5X-ray3.10B1-785[»]
3TEHX-ray2.85B1-785[»]
4TVAX-ray2.60B1-785[»]
ProteinModelPortaliP27002.
SMRiP27002.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27002.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 147tRNA-bindingAdd BLAST109
Domaini399 – 474B5Add BLAST76
Domaini688 – 780FDX-ACBAdd BLAST93

Sequence similaritiesi

Contains 1 B5 domain.Curated
Contains 1 FDX-ACB domain.Curated
Contains 1 tRNA-binding domain.Curated

Phylogenomic databases

eggNOGiENOG4105C6A. Bacteria.
COG0072. LUCA.

Family and domain databases

CDDicd02796. tRNA_bind_bactPheRS. 1 hit.
Gene3Di3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR033714. tRNA_bind_bactPheRS.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 3 hits.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVPFSWLKA YVPELESPEV LEERLAGLGF ETDRIERVFP IPRGVVFARV
60 70 80 90 100
LEAHPIPGTR LKRLVLDAGR TVEVVSGAEN ARKGIGVALA LPGTELPGLG
110 120 130 140 150
QKVGERVIQG VRSFGMALSP RELGVGEYGG GLLEFPEDAL PPGTPLSEAW
160 170 180 190 200
PEEVVLDLEV TPNRPDALGL LGLARDLHAL GYALVEPEAA LKAEALPLPF
210 220 230 240 250
ALKVEDPEGA PHFTLGYAFG LRVAPSPLWM QRALFAAGMR PINNVVDVTN
260 270 280 290 300
YVMLERAQPM HAFDLRFVGE GIAVRRAREG ERLKTLDGVE RTLHPEDLVI
310 320 330 340 350
AGWRGEESFP LGLAGVMGGA ESEVREDTEA IALEVACFDP VSIRKTARRH
360 370 380 390 400
GLRTEASHRF ERGVDPLGQV PAQRRALSLL QALAGARVAE ALLEAGSPKP
410 420 430 440 450
PEAIPFRPEY ANRLLGTSYP EAEQIAILKR LGCRVEGEGP TYRVTPPSHR
460 470 480 490 500
LDLRLEEDLV EEVARIQGYE TIPLALPAFF PAPDNRGVEA PYRKEQRLRE
510 520 530 540 550
VLSGLGFQEV YTYSFMDPED ARRFRLDPPR LLLLNPLAPE KAALRTHLFP
560 570 580 590 600
GLVRVLKENL DLDRPERALL FEVGRVFRER EETHLAGLLF GEGVGLPWAK
610 620 630 640 650
ERLSGYFLLK GYLEALFARL GLAFRVEAQA FPFLHPGVSG RVLVEGEEVG
660 670 680 690 700
FLGALHPEIA QELELPPVHL FELRLPLPDK PLAFQDPSRH PAAFRDLAVV
710 720 730 740 750
VPAPTPYGEV EALVREAAGP YLESLALFDL YQGPPLPEGH KSLAFHLRFR
760 770 780
HPKRTLRDEE VEEAVSRVAE ALRARGFGLR GLDTP
Length:785
Mass (Da):86,611
Last modified:December 1, 1992 - v2
Checksum:iBDC51952DE8E0F00
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B70X-ray2.70B1-785[»]
1B7YX-ray2.50B1-785[»]
2AKWX-ray2.80B1-785[»]
2ALYX-ray2.60B1-785[»]
2AMCX-ray2.70B1-785[»]
2IY5X-ray3.10B1-785[»]
3TEHX-ray2.85B1-785[»]
4TVAX-ray2.60B1-785[»]
ProteinModelPortaliP27002.
SMRiP27002.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6105N.
MINTiMINT-88295.
STRINGi262724.TTC1595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C6A. Bacteria.
COG0072. LUCA.

Miscellaneous databases

EvolutionaryTraceiP27002.

Family and domain databases

CDDicd02796. tRNA_bind_bactPheRS. 1 hit.
Gene3Di3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR033714. tRNA_bind_bactPheRS.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 3 hits.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFB_THETH
AccessioniPrimary (citable) accession number: P27002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The sequence shown here has been extracted from PDB entry 1B70.Curated

Keywords - Technical termi

3D-structure

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.