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P27002

- SYFB_THETH

UniProt

P27002 - SYFB_THETH

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Thermus thermophilus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

    Cofactori

    Binds 2 magnesium ions per tetramer.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi452 – 4521Magnesium
    Metal bindingi458 – 4581Magnesium; via carbonyl oxygen
    Metal bindingi461 – 4611Magnesium
    Metal bindingi462 – 4621Magnesium

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunit
    Short name:
    PheRS
    Gene namesi
    Name:pheT
    OrganismiThermus thermophilus
    Taxonomic identifieri274 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 785785Phenylalanine--tRNA ligase beta subunitPRO_0000126978Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.

    Protein-protein interaction databases

    DIPiDIP-6105N.
    MINTiMINT-88295.
    STRINGi262724.TTC1595.

    Structurei

    Secondary structure

    1
    785
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 43
    Helixi5 – 84
    Turni9 – 113
    Helixi18 – 2811
    Beta strandi34 – 374
    Beta strandi45 – 5511
    Turni57 – 604
    Beta strandi62 – 7615
    Beta strandi86 – 905
    Turni97 – 993
    Beta strandi100 – 1023
    Beta strandi105 – 1084
    Beta strandi109 – 1135
    Beta strandi115 – 1173
    Helixi120 – 1234
    Beta strandi125 – 1273
    Helixi146 – 1483
    Beta strandi154 – 1574
    Helixi165 – 1684
    Helixi170 – 17910
    Beta strandi199 – 2057
    Turni207 – 2093
    Beta strandi211 – 22010
    Helixi228 – 2369
    Helixi244 – 25512
    Beta strandi261 – 2644
    Helixi265 – 2673
    Beta strandi271 – 2766
    Beta strandi282 – 2854
    Beta strandi290 – 2923
    Beta strandi298 – 3047
    Beta strandi307 – 3137
    Turni314 – 3163
    Beta strandi317 – 3193
    Turni320 – 3223
    Beta strandi330 – 3378
    Helixi340 – 34910
    Helixi355 – 3628
    Helixi369 – 38315
    Beta strandi387 – 3904
    Beta strandi393 – 3953
    Beta strandi404 – 4063
    Helixi408 – 4158
    Helixi421 – 43010
    Beta strandi434 – 44512
    Helixi456 – 46712
    Helixi469 – 4713
    Helixi483 – 4853
    Turni486 – 4894
    Helixi490 – 50516
    Beta strandi514 – 5163
    Helixi520 – 5234
    Beta strandi532 – 5354
    Beta strandi541 – 5444
    Helixi549 – 56214
    Beta strandi568 – 59225
    Beta strandi597 – 6004
    Helixi606 – 62015
    Beta strandi625 – 6273
    Beta strandi634 – 64411
    Beta strandi647 – 6559
    Helixi657 – 6626
    Beta strandi669 – 6768
    Beta strandi693 – 6975
    Beta strandi701 – 7055
    Helixi707 – 71812
    Beta strandi722 – 7298
    Beta strandi733 – 7364
    Helixi737 – 7393
    Beta strandi744 – 7496
    Beta strandi752 – 7543
    Helixi758 – 77316
    Turni774 – 7763
    Beta strandi782 – 7843

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B70X-ray2.70B1-785[»]
    1B7YX-ray2.50B1-785[»]
    2AKWX-ray2.80B1-785[»]
    2ALYX-ray2.60B1-785[»]
    2AMCX-ray2.70B1-785[»]
    2IY5X-ray3.10B1-785[»]
    3TEHX-ray2.85B1-785[»]
    ProteinModelPortaliP27002.
    SMRiP27002. Positions 1-785.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP27002.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 147109tRNA-bindingAdd
    BLAST
    Domaini399 – 47476B5Add
    BLAST
    Domaini688 – 78093FDX-ACBAdd
    BLAST

    Sequence similaritiesi

    Contains 1 B5 domain.Curated
    Contains 1 FDX-ACB domain.Curated
    Contains 1 tRNA-binding domain.Curated

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR002319. Phenylalanyl-tRNA_Synthase.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01409. tRNA-synt_2d. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 3 hits.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27002-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVPFSWLKA YVPELESPEV LEERLAGLGF ETDRIERVFP IPRGVVFARV    50
    LEAHPIPGTR LKRLVLDAGR TVEVVSGAEN ARKGIGVALA LPGTELPGLG 100
    QKVGERVIQG VRSFGMALSP RELGVGEYGG GLLEFPEDAL PPGTPLSEAW 150
    PEEVVLDLEV TPNRPDALGL LGLARDLHAL GYALVEPEAA LKAEALPLPF 200
    ALKVEDPEGA PHFTLGYAFG LRVAPSPLWM QRALFAAGMR PINNVVDVTN 250
    YVMLERAQPM HAFDLRFVGE GIAVRRAREG ERLKTLDGVE RTLHPEDLVI 300
    AGWRGEESFP LGLAGVMGGA ESEVREDTEA IALEVACFDP VSIRKTARRH 350
    GLRTEASHRF ERGVDPLGQV PAQRRALSLL QALAGARVAE ALLEAGSPKP 400
    PEAIPFRPEY ANRLLGTSYP EAEQIAILKR LGCRVEGEGP TYRVTPPSHR 450
    LDLRLEEDLV EEVARIQGYE TIPLALPAFF PAPDNRGVEA PYRKEQRLRE 500
    VLSGLGFQEV YTYSFMDPED ARRFRLDPPR LLLLNPLAPE KAALRTHLFP 550
    GLVRVLKENL DLDRPERALL FEVGRVFRER EETHLAGLLF GEGVGLPWAK 600
    ERLSGYFLLK GYLEALFARL GLAFRVEAQA FPFLHPGVSG RVLVEGEEVG 650
    FLGALHPEIA QELELPPVHL FELRLPLPDK PLAFQDPSRH PAAFRDLAVV 700
    VPAPTPYGEV EALVREAAGP YLESLALFDL YQGPPLPEGH KSLAFHLRFR 750
    HPKRTLRDEE VEEAVSRVAE ALRARGFGLR GLDTP 785
    Length:785
    Mass (Da):86,611
    Last modified:December 1, 1992 - v2
    Checksum:iBDC51952DE8E0F00
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B70 X-ray 2.70 B 1-785 [» ]
    1B7Y X-ray 2.50 B 1-785 [» ]
    2AKW X-ray 2.80 B 1-785 [» ]
    2ALY X-ray 2.60 B 1-785 [» ]
    2AMC X-ray 2.70 B 1-785 [» ]
    2IY5 X-ray 3.10 B 1-785 [» ]
    3TEH X-ray 2.85 B 1-785 [» ]
    ProteinModelPortali P27002.
    SMRi P27002. Positions 1-785.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6105N.
    MINTi MINT-88295.
    STRINGi 262724.TTC1595.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P27002.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR002319. Phenylalanyl-tRNA_Synthase.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01409. tRNA-synt_2d. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 3 hits.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Crystal structures of phenylalanyl-tRNA synthetase complexed with phenylalanine and a phenylalanyl-adenylate analogue."
      Reshetnikova L., Moor N., Lavrik O., Vassylyev D.G.
      J. Mol. Biol. 287:555-568(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

    Entry informationi

    Entry nameiSYFB_THETH
    AccessioniPrimary (citable) accession number: P27002
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    The sequence shown here has been extracted from PDB entry 1B70.Curated

    Keywords - Technical termi

    3D-structure

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3