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Protein

Glucoamylase GLA1

Gene

GLA1

Organism
Saccharomycopsis fibuligera (Yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei166 – 1661SubstrateBy similarity
Active sitei234 – 2341Proton acceptorPROSITE-ProRule annotation
Active sitei237 – 2371Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase GLA1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GLA1
OrganismiSaccharomycopsis fibuligera (Yeast)
Taxonomic identifieri4944 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycopsidaceaeSaccharomycopsis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Add
BLAST
Chaini28 – 519492Glucoamylase GLA1PRO_0000001476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP26989.
SMRiP26989. Positions 28-519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26989-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFGVLISVF VAIVSALPLQ EGPLNKRAYP SFEAYSNYKV DRTDLETFLD
60 70 80 90 100
KQKDVSLYYL LQNIAYPEGQ FNDGVPGTVI ASPSTSNPDY YYQWTRDSAI
110 120 130 140 150
TFLTVLSELE DNNFNTTLAK AVEYYINTSY NLQRTSNPSG SFDDENHKGL
160 170 180 190 200
GEPKFNTDGS AYTGAWGRPQ NDGPALRAYA ISRYLNDVNS LNKGKLVLTD
210 220 230 240 250
SGDINFSSTE DIYKNIIKPD LEYVIGYWDS TGFDLWEENQ GRHFFTSLVQ
260 270 280 290 300
QKALAYAVDI AKSFDDGDFA NTLSSTASTL ESYLSGSDGG FVNTDVNHIV
310 320 330 340 350
ENPDLLQQNS RQGLDSATYI GPLLTHDIGE SSSTPFDVDN EYVLQSYYLL
360 370 380 390 400
LEDNKDRYSV NSAYSAGAAI GRYPEDVYNG DGSSEGNPWF LATAYAAQVP
410 420 430 440 450
YKLVYDAKSA SNDITINKIN YDFFNKYIVD LSTINSGYQS SDSVTIKSGS
460 470 480 490 500
DEFNTVADNL VTFGDSFLQV ILDHINDDGS LNEQLNRNTG YSTSAYSLTW
510
SSGALLEAIR LRNKVKALA
Length:519
Mass (Da):57,542
Last modified:July 15, 1998 - v2
Checksum:iA15A009A7640053C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58117 Genomic DNA. Translation: CAA41120.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58117 Genomic DNA. Translation: CAA41120.1.

3D structure databases

ProteinModelPortaliP26989.
SMRiP26989. Positions 28-519.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The nucleotide sequence of the glucoamylase gene GLA1 from Saccharomycopsis fibuligera KZ."
    Hostinova E., Balanova J., Gasperik J.
    FEMS Microbiol. Lett. 67:103-108(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KZ.
  2. Hostinova E.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Strain: KZ.

Entry informationi

Entry nameiAMYH_SACFI
AccessioniPrimary (citable) accession number: P26989
Secondary accession number(s): P78745
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 15, 1998
Last modified: October 1, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.