P26989 (AMYH_SACFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucoamylase GLA1
EC=3.2.1.3

Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase

Gene names

Name:

GLA1

Organism

Saccharomycopsis fibuligera (Yeast)

Taxonomic identifier

4944 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycopsidaceae › Saccharomycopsis

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 15 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glucan 1,4-alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27
	Chain	28 – 519	492	Glucoamylase GLA1		PRO_0000001476
Sites
	Active site	234	1	Proton acceptor By similarity
	Active site	237	1	Proton donor By similarity
	Binding site	166	1	Substrate By similarity
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	205	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P26989 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: A15A009A7640053C	FASTA	519	57,542
	10 20 30 40 50 60 MRFGVLISVF VAIVSALPLQ EGPLNKRAYP SFEAYSNYKV DRTDLETFLD KQKDVSLYYL 70 80 90 100 110 120 LQNIAYPEGQ FNDGVPGTVI ASPSTSNPDY YYQWTRDSAI TFLTVLSELE DNNFNTTLAK 130 140 150 160 170 180 AVEYYINTSY NLQRTSNPSG SFDDENHKGL GEPKFNTDGS AYTGAWGRPQ NDGPALRAYA 190 200 210 220 230 240 ISRYLNDVNS LNKGKLVLTD SGDINFSSTE DIYKNIIKPD LEYVIGYWDS TGFDLWEENQ 250 260 270 280 290 300 GRHFFTSLVQ QKALAYAVDI AKSFDDGDFA NTLSSTASTL ESYLSGSDGG FVNTDVNHIV 310 320 330 340 350 360 ENPDLLQQNS RQGLDSATYI GPLLTHDIGE SSSTPFDVDN EYVLQSYYLL LEDNKDRYSV 370 380 390 400 410 420 NSAYSAGAAI GRYPEDVYNG DGSSEGNPWF LATAYAAQVP YKLVYDAKSA SNDITINKIN 430 440 450 460 470 480 YDFFNKYIVD LSTINSGYQS SDSVTIKSGS DEFNTVADNL VTFGDSFLQV ILDHINDDGS 490 500 510 LNEQLNRNTG YSTSAYSLTW SSGALLEAIR LRNKVKALA

References

[1]	"The nucleotide sequence of the glucoamylase gene GLA1 from Saccharomycopsis fibuligera KZ." Hostinova E., Balanova J., Gasperik J. FEMS Microbiol. Lett. 67:103-108(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KZ.
[2]	Hostinova E. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. Strain: KZ.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58117 Genomic DNA. Translation: CAA41120.1.
3D structure databases
ProteinModelPortal	P26989.
SMR	P26989. Positions 28-519.
ModBase	Search...
Protein family/group databases
CAZy	GH15. Glycoside Hydrolase Family 15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR000165. Glucoamylase. IPR011613. Glyco_hydro_15. [Graphical view]
Pfam	PF00723. Glyco_hydro_15. 1 hit. [Graphical view]
PRINTS	PR00736. GLHYDRLASE15.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00820. GLUCOAMYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYH_SACFI

Accession

Primary (citable) accession number: P26989
Secondary accession number(s): P78745

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program