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P26989

- AMYH_SACFI

UniProt

P26989 - AMYH_SACFI

Protein

Glucoamylase GLA1

Gene

GLA1

Organism
Saccharomycopsis fibuligera (Yeast)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei166 – 1661SubstrateBy similarity
    Active sitei234 – 2341Proton acceptorPROSITE-ProRule annotation
    Active sitei237 – 2371Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH15. Glycoside Hydrolase Family 15.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucoamylase GLA1 (EC:3.2.1.3)
    Alternative name(s):
    1,4-alpha-D-glucan glucohydrolase
    Glucan 1,4-alpha-glucosidase
    Gene namesi
    Name:GLA1
    OrganismiSaccharomycopsis fibuligera (Yeast)
    Taxonomic identifieri4944 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycopsidaceaeSaccharomycopsis

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Add
    BLAST
    Chaini28 – 519492Glucoamylase GLA1PRO_0000001476Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliP26989.
    SMRiP26989. Positions 28-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 15 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view]
    PfamiPF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    PRINTSiPR00736. GLHYDRLASE15.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26989-1 [UniParc]FASTAAdd to Basket

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    MRFGVLISVF VAIVSALPLQ EGPLNKRAYP SFEAYSNYKV DRTDLETFLD    50
    KQKDVSLYYL LQNIAYPEGQ FNDGVPGTVI ASPSTSNPDY YYQWTRDSAI 100
    TFLTVLSELE DNNFNTTLAK AVEYYINTSY NLQRTSNPSG SFDDENHKGL 150
    GEPKFNTDGS AYTGAWGRPQ NDGPALRAYA ISRYLNDVNS LNKGKLVLTD 200
    SGDINFSSTE DIYKNIIKPD LEYVIGYWDS TGFDLWEENQ GRHFFTSLVQ 250
    QKALAYAVDI AKSFDDGDFA NTLSSTASTL ESYLSGSDGG FVNTDVNHIV 300
    ENPDLLQQNS RQGLDSATYI GPLLTHDIGE SSSTPFDVDN EYVLQSYYLL 350
    LEDNKDRYSV NSAYSAGAAI GRYPEDVYNG DGSSEGNPWF LATAYAAQVP 400
    YKLVYDAKSA SNDITINKIN YDFFNKYIVD LSTINSGYQS SDSVTIKSGS 450
    DEFNTVADNL VTFGDSFLQV ILDHINDDGS LNEQLNRNTG YSTSAYSLTW 500
    SSGALLEAIR LRNKVKALA 519
    Length:519
    Mass (Da):57,542
    Last modified:July 15, 1998 - v2
    Checksum:iA15A009A7640053C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58117 Genomic DNA. Translation: CAA41120.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58117 Genomic DNA. Translation: CAA41120.1 .

    3D structure databases

    ProteinModelPortali P26989.
    SMRi P26989. Positions 28-519.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH15. Glycoside Hydrolase Family 15.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view ]
    Pfami PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    PRINTSi PR00736. GLHYDRLASE15.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of the glucoamylase gene GLA1 from Saccharomycopsis fibuligera KZ."
      Hostinova E., Balanova J., Gasperik J.
      FEMS Microbiol. Lett. 67:103-108(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KZ.
    2. Hostinova E.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
      Strain: KZ.

    Entry informationi

    Entry nameiAMYH_SACFI
    AccessioniPrimary (citable) accession number: P26989
    Secondary accession number(s): P78745
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3