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Protein

Glucoamylase GLA1

Gene

GLA1

Organism
Saccharomycopsis fibuligera (Yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166SubstrateBy similarity1
Active sitei234Proton acceptorPROSITE-ProRule annotation1
Active sitei237Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase GLA1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GLA1
OrganismiSaccharomycopsis fibuligera (Yeast)
Taxonomic identifieri4944 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycopsidaceaeSaccharomycopsis

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Add BLAST27
ChainiPRO_000000147628 – 519Glucoamylase GLA1Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi115N-linked (GlcNAc...)Sequence analysis1
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi205N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP26989.
SMRiP26989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiView protein in InterPro
IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
PfamiView protein in Pfam
PF00723. Glyco_hydro_15. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiView protein in PROSITE
PS00820. GLUCOAMYLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFGVLISVF VAIVSALPLQ EGPLNKRAYP SFEAYSNYKV DRTDLETFLD
60 70 80 90 100
KQKDVSLYYL LQNIAYPEGQ FNDGVPGTVI ASPSTSNPDY YYQWTRDSAI
110 120 130 140 150
TFLTVLSELE DNNFNTTLAK AVEYYINTSY NLQRTSNPSG SFDDENHKGL
160 170 180 190 200
GEPKFNTDGS AYTGAWGRPQ NDGPALRAYA ISRYLNDVNS LNKGKLVLTD
210 220 230 240 250
SGDINFSSTE DIYKNIIKPD LEYVIGYWDS TGFDLWEENQ GRHFFTSLVQ
260 270 280 290 300
QKALAYAVDI AKSFDDGDFA NTLSSTASTL ESYLSGSDGG FVNTDVNHIV
310 320 330 340 350
ENPDLLQQNS RQGLDSATYI GPLLTHDIGE SSSTPFDVDN EYVLQSYYLL
360 370 380 390 400
LEDNKDRYSV NSAYSAGAAI GRYPEDVYNG DGSSEGNPWF LATAYAAQVP
410 420 430 440 450
YKLVYDAKSA SNDITINKIN YDFFNKYIVD LSTINSGYQS SDSVTIKSGS
460 470 480 490 500
DEFNTVADNL VTFGDSFLQV ILDHINDDGS LNEQLNRNTG YSTSAYSLTW
510
SSGALLEAIR LRNKVKALA
Length:519
Mass (Da):57,542
Last modified:July 15, 1998 - v2
Checksum:iA15A009A7640053C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58117 Genomic DNA. Translation: CAA41120.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58117 Genomic DNA. Translation: CAA41120.1.

3D structure databases

ProteinModelPortaliP26989.
SMRiP26989.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiView protein in InterPro
IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
PfamiView protein in Pfam
PF00723. Glyco_hydro_15. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiView protein in PROSITE
PS00820. GLUCOAMYLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYH_SACFI
AccessioniPrimary (citable) accession number: P26989
Secondary accession number(s): P78745
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 15, 1998
Last modified: March 15, 2017
This is version 72 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.