Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Interleukin-3 receptor subunit alpha

Gene

Il3ra

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

In mouse, there are two classes of high-affinity IL3 receptors. One contains an IL3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL5 and GM-CSF receptors.

GO - Molecular functioni

  1. cytokine receptor activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-3 receptor subunit alpha
Short name:
IL-3 receptor subunit alpha
Short name:
IL-3R subunit alpha
Short name:
IL-3R-alpha
Short name:
IL-3RA
Alternative name(s):
Interleukin-3 receptor class II alpha chain
CD_antigen: CD123
Gene namesi
Name:Il3ra
Synonyms:Sut-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:96553. Il3ra.

Subcellular locationi

Isoform 1 : Cell membrane; Single-pass type I membrane protein
Note: Expressed on the cell surface.
Isoform 2 : Endomembrane system; Single-pass type I membrane protein
Note: Mostly distributed inside the cells, except in the nuclei and is not transported to the cell surface.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 331315ExtracellularSequence AnalysisAdd
BLAST
Transmembranei332 – 35524HelicalSequence AnalysisAdd
BLAST
Topological domaini356 – 39641CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endomembrane system Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 396380Interleukin-3 receptor subunit alphaPRO_0000010884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP26952.

PTM databases

PhosphoSiteiP26952.

Expressioni

Gene expression databases

BgeeiP26952.
CleanExiMM_IL3RA.
GenevestigatoriP26952.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Protein-protein interaction databases

BioGridi200636. 3 interactions.
DIPiDIP-59621N.
STRINGi10090.ENSMUSP00000088079.

Structurei

3D structure databases

ProteinModelPortaliP26952.
SMRiP26952. Positions 33-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi312 – 3165WSXWS motif
Motifi363 – 3719Box 1 motif

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG44788.
GeneTreeiENSGT00520000055993.
HOVERGENiHBG052115.
InParanoidiP26952.
KOiK04737.
OMAiKNLRMEP.
OrthoDBiEOG7TJ3J7.
PhylomeDBiP26952.
TreeFamiTF331549.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR015321. IL-6_rcpt_alpha-bd.
IPR003532. Short_hematopoietin_rcpt_2_CS.
[Graphical view]
PfamiPF09240. IL6Ra-bind. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS01356. HEMATOPO_REC_S_F2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P26952-1) [UniParc]FASTAAdd to basket

Also known as: B, SP1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAANLWLILG LLASHSSDLA AVREAPPTAV TTPIQNLHID PAHYTLSWDP
60 70 80 90 100
APGADITTGA FCRKGRDIFV WADPGLARCS FQSLSLCHVT NFTVFLGKDR
110 120 130 140 150
AVAGSIQFPP DDDGDHEAAA QDLRCWVHEG QLSCQWERGP KATGDVHYRM
160 170 180 190 200
FWRDVRLGPA HNRECPHYHS LDVNTAGPAP HGGHEGCTLD LDTVLGSTPN
210 220 230 240 250
SPDLVPQVTI TVNGSGRAGP VPCMDNTVDL QRAEVLAPPT LTVECNGSEA
260 270 280 290 300
HARWVARNRF HHGLLGYTLQ VNQSSRSEPQ EYNVSIPHFW VPNAGAISFR
310 320 330 340 350
VKSRSEVYPR KLSSWSEAWG LVCPPEVMPV KTALVTSVAT VLGAGLVAAG
360 370 380 390
LLLWWRKSLL YRLCPPIPRL RLPLAGEMVV WEPALEDCEV TPVTDA
Length:396
Mass (Da):43,195
Last modified:September 30, 1993 - v1
Checksum:iF1A5BF11743AB8A3
GO
Isoform 2 (identifier: P26952-3) [UniParc]FASTAAdd to basket

Also known as: SP2

The sequence of this isoform differs from the canonical sequence as follows:
     20-112: Missing.

Show »
Length:303
Mass (Da):33,203
Checksum:i044AE7DB3E1E2587
GO
Isoform 3 (identifier: P26952-2) [UniParc]FASTAAdd to basket

Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     274-283: Missing.

Show »
Length:386
Mass (Da):42,017
Checksum:i9E383407D88AD774
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131D → G no nucleotide entry (PubMed:7889941).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei20 – 11293Missing in isoform 2. 1 PublicationVSP_040623Add
BLAST
Alternative sequencei274 – 28310Missing in isoform 3. 1 PublicationVSP_011265

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64534 mRNA. Translation: CAA45833.1.
FJ550346 mRNA. Translation: ACM24115.1.
CCDSiCCDS26827.1. [P26952-1]
PIRiS22909.
RefSeqiNP_032395.1. NM_008369.1. [P26952-1]
XP_006517998.2. XM_006517935.2.
XP_006518004.2. XM_006517941.2.
UniGeneiMm.425857.

Genome annotation databases

EnsembliENSMUST00000090591; ENSMUSP00000088079; ENSMUSG00000068758. [P26952-1]
GeneIDi16188.
KEGGimmu:16188.
UCSCiuc007sgm.1. mouse. [P26952-1]
uc011zgb.1. mouse. [P26952-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64534 mRNA. Translation: CAA45833.1.
FJ550346 mRNA. Translation: ACM24115.1.
CCDSiCCDS26827.1. [P26952-1]
PIRiS22909.
RefSeqiNP_032395.1. NM_008369.1. [P26952-1]
XP_006517998.2. XM_006517935.2.
XP_006518004.2. XM_006517941.2.
UniGeneiMm.425857.

3D structure databases

ProteinModelPortaliP26952.
SMRiP26952. Positions 33-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200636. 3 interactions.
DIPiDIP-59621N.
STRINGi10090.ENSMUSP00000088079.

PTM databases

PhosphoSiteiP26952.

Proteomic databases

PRIDEiP26952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090591; ENSMUSP00000088079; ENSMUSG00000068758. [P26952-1]
GeneIDi16188.
KEGGimmu:16188.
UCSCiuc007sgm.1. mouse. [P26952-1]
uc011zgb.1. mouse. [P26952-3]

Organism-specific databases

CTDi3563.
MGIiMGI:96553. Il3ra.

Phylogenomic databases

eggNOGiNOG44788.
GeneTreeiENSGT00520000055993.
HOVERGENiHBG052115.
InParanoidiP26952.
KOiK04737.
OMAiKNLRMEP.
OrthoDBiEOG7TJ3J7.
PhylomeDBiP26952.
TreeFamiTF331549.

Miscellaneous databases

NextBioi289089.
PROiP26952.
SOURCEiSearch...

Gene expression databases

BgeeiP26952.
CleanExiMM_IL3RA.
GenevestigatoriP26952.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR015321. IL-6_rcpt_alpha-bd.
IPR003532. Short_hematopoietin_rcpt_2_CS.
[Graphical view]
PfamiPF09240. IL6Ra-bind. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS01356. HEMATOPO_REC_S_F2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two distinct functional high affinity receptors for mouse interleukin-3 (IL-3)."
    Hara T., Miyajima A.
    EMBO J. 11:1875-1884(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: B6.S.
  2. "Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene."
    Ichihara M., Hara T., Takagi M., Cho L.C., Gorman D.M., Miyajima A.
    EMBO J. 14:939-950(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), SUBCELLULAR LOCATION.
    Strain: A/J and C57BL/6.
  3. "A new isoform of IL-3 receptor alpha with novel differentiation activity and high affinity binding mode."
    Chen J., Olsen J., Ford S., Mirza S., Walker A., Murphy J.M., Young I.G.
    J. Biol. Chem. 284:5763-5773(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.

Entry informationi

Entry nameiIL3RA_MOUSE
AccessioniPrimary (citable) accession number: P26952
Secondary accession number(s): B9VI80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1993
Last sequence update: September 30, 1993
Last modified: March 31, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.