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P26952 (IL3RA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-3 receptor subunit alpha

Short name=IL-3 receptor subunit alpha
Short name=IL-3R subunit alpha
Short name=IL-3R-alpha
Short name=IL-3RA
Alternative name(s):
Interleukin-3 receptor class II alpha chain
CD_antigen=CD123
Gene names
Name:Il3ra
Synonyms:Sut-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In mouse, there are two classes of high-affinity IL3 receptors. One contains an IL3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL5 and GM-CSF receptors.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: Expressed on the cell surface. Ref.2

Isoform 2: Endomembrane system; Single-pass type I membrane protein. Note: Mostly distributed inside the cells, except in the nuclei and is not transported to the cell surface. Ref.2

Domain

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentendomembrane system

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncytokine receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P26952-1)

Also known as: B; SP1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P26952-3)

Also known as: SP2;

The sequence of this isoform differs from the canonical sequence as follows:
     20-112: Missing.
Isoform 3 (identifier: P26952-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     274-283: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 396380Interleukin-3 receptor subunit alpha
PRO_0000010884

Regions

Topological domain17 – 331315Extracellular Potential
Transmembrane332 – 35524Helical; Potential
Topological domain356 – 39641Cytoplasmic Potential
Motif312 – 3165WSXWS motif
Motif363 – 3719Box 1 motif

Amino acid modifications

Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation2131N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Glycosylation2721N-linked (GlcNAc...) Potential
Glycosylation2831N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence20 – 11293Missing in isoform 2.
VSP_040623
Alternative sequence274 – 28310Missing in isoform 3.
VSP_011265

Experimental info

Sequence conflict1131D → G no nucleotide entry Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (B) (SP1) [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: F1A5BF11743AB8A3

FASTA39643,195
        10         20         30         40         50         60 
MAANLWLILG LLASHSSDLA AVREAPPTAV TTPIQNLHID PAHYTLSWDP APGADITTGA 

        70         80         90        100        110        120 
FCRKGRDIFV WADPGLARCS FQSLSLCHVT NFTVFLGKDR AVAGSIQFPP DDDGDHEAAA 

       130        140        150        160        170        180 
QDLRCWVHEG QLSCQWERGP KATGDVHYRM FWRDVRLGPA HNRECPHYHS LDVNTAGPAP 

       190        200        210        220        230        240 
HGGHEGCTLD LDTVLGSTPN SPDLVPQVTI TVNGSGRAGP VPCMDNTVDL QRAEVLAPPT 

       250        260        270        280        290        300 
LTVECNGSEA HARWVARNRF HHGLLGYTLQ VNQSSRSEPQ EYNVSIPHFW VPNAGAISFR 

       310        320        330        340        350        360 
VKSRSEVYPR KLSSWSEAWG LVCPPEVMPV KTALVTSVAT VLGAGLVAAG LLLWWRKSLL 

       370        380        390 
YRLCPPIPRL RLPLAGEMVV WEPALEDCEV TPVTDA 

« Hide

Isoform 2 (SP2) [UniParc].

Checksum: 044AE7DB3E1E2587
Show »

FASTA30333,203
Isoform 3 (A) [UniParc].

Checksum: 9E383407D88AD774
Show »

FASTA38642,017

References

[1]"Two distinct functional high affinity receptors for mouse interleukin-3 (IL-3)."
Hara T., Miyajima A.
EMBO J. 11:1875-1884(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: B6.S.
[2]"Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene."
Ichihara M., Hara T., Takagi M., Cho L.C., Gorman D.M., Miyajima A.
EMBO J. 14:939-950(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), SUBCELLULAR LOCATION.
Strain: A/J and C57BL/6.
[3]"A new isoform of IL-3 receptor alpha with novel differentiation activity and high affinity binding mode."
Chen J., Olsen J., Ford S., Mirza S., Walker A., Murphy J.M., Young I.G.
J. Biol. Chem. 284:5763-5773(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X64534 mRNA. Translation: CAA45833.1.
FJ550346 mRNA. Translation: ACM24115.1.
CCDSCCDS26827.1. [P26952-1]
PIRS22909.
RefSeqNP_032395.1. NM_008369.1. [P26952-1]
UniGeneMm.425857.

3D structure databases

ProteinModelPortalP26952.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200636. 3 interactions.
DIPDIP-59621N.
STRING10090.ENSMUSP00000088079.

PTM databases

PhosphoSiteP26952.

Proteomic databases

PRIDEP26952.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000090591; ENSMUSP00000088079; ENSMUSG00000068758. [P26952-1]
GeneID16188.
KEGGmmu:16188.
UCSCuc007sgm.1. mouse. [P26952-1]
uc011zgb.1. mouse. [P26952-3]

Organism-specific databases

CTD3563.
MGIMGI:96553. Il3ra.

Phylogenomic databases

eggNOGNOG44788.
GeneTreeENSGT00520000055993.
HOVERGENHBG052115.
InParanoidP26952.
KOK04737.
OMAKNLRMEP.
OrthoDBEOG7TJ3J7.
PhylomeDBP26952.
TreeFamTF331549.

Gene expression databases

BgeeP26952.
CleanExMM_IL3RA.
GenevestigatorP26952.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR015321. IL-6_rcpt_alpha-bd.
IPR003532. Short_hematopoietin_rcpt_2_CS.
[Graphical view]
PfamPF09240. IL6Ra-bind. 1 hit.
[Graphical view]
SUPFAMSSF49265. SSF49265. 1 hit.
PROSITEPS01356. HEMATOPO_REC_S_F2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio289089.
PROP26952.
SOURCESearch...

Entry information

Entry nameIL3RA_MOUSE
AccessionPrimary (citable) accession number: P26952
Secondary accession number(s): B9VI80
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot