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P26952

- IL3RA_MOUSE

UniProt

P26952 - IL3RA_MOUSE

Protein

Interleukin-3 receptor subunit alpha

Gene

Il3ra

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    In mouse, there are two classes of high-affinity IL3 receptors. One contains an IL3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL5 and GM-CSF receptors.

    GO - Molecular functioni

    1. cytokine receptor activity Source: InterPro

    Keywords - Molecular functioni

    Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin-3 receptor subunit alpha
    Short name:
    IL-3 receptor subunit alpha
    Short name:
    IL-3R subunit alpha
    Short name:
    IL-3R-alpha
    Short name:
    IL-3RA
    Alternative name(s):
    Interleukin-3 receptor class II alpha chain
    CD_antigen: CD123
    Gene namesi
    Name:Il3ra
    Synonyms:Sut-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:96553. Il3ra.

    Subcellular locationi

    Isoform 1 : Cell membrane; Single-pass type I membrane protein
    Note: Expressed on the cell surface.
    Isoform 2 : Endomembrane system; Single-pass type I membrane protein
    Note: Mostly distributed inside the cells, except in the nuclei and is not transported to the cell surface.

    GO - Cellular componenti

    1. endomembrane system Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 396380Interleukin-3 receptor subunit alphaPRO_0000010884Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP26952.

    PTM databases

    PhosphoSiteiP26952.

    Expressioni

    Gene expression databases

    BgeeiP26952.
    CleanExiMM_IL3RA.
    GenevestigatoriP26952.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit.

    Protein-protein interaction databases

    BioGridi200636. 3 interactions.
    DIPiDIP-59621N.
    STRINGi10090.ENSMUSP00000088079.

    Structurei

    3D structure databases

    ProteinModelPortaliP26952.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 331315ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini356 – 39641CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei332 – 35524HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi312 – 3165WSXWS motif
    Motifi363 – 3719Box 1 motif

    Domaini

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG44788.
    GeneTreeiENSGT00520000055993.
    HOVERGENiHBG052115.
    InParanoidiP26952.
    KOiK04737.
    OMAiKNLRMEP.
    OrthoDBiEOG7TJ3J7.
    PhylomeDBiP26952.
    TreeFamiTF331549.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR003961. Fibronectin_type3.
    IPR013783. Ig-like_fold.
    IPR015321. IL-6_rcpt_alpha-bd.
    IPR003532. Short_hematopoietin_rcpt_2_CS.
    [Graphical view]
    PfamiPF09240. IL6Ra-bind. 1 hit.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 1 hit.
    PROSITEiPS01356. HEMATOPO_REC_S_F2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P26952-1) [UniParc]FASTAAdd to Basket

    Also known as: B, SP1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAANLWLILG LLASHSSDLA AVREAPPTAV TTPIQNLHID PAHYTLSWDP    50
    APGADITTGA FCRKGRDIFV WADPGLARCS FQSLSLCHVT NFTVFLGKDR 100
    AVAGSIQFPP DDDGDHEAAA QDLRCWVHEG QLSCQWERGP KATGDVHYRM 150
    FWRDVRLGPA HNRECPHYHS LDVNTAGPAP HGGHEGCTLD LDTVLGSTPN 200
    SPDLVPQVTI TVNGSGRAGP VPCMDNTVDL QRAEVLAPPT LTVECNGSEA 250
    HARWVARNRF HHGLLGYTLQ VNQSSRSEPQ EYNVSIPHFW VPNAGAISFR 300
    VKSRSEVYPR KLSSWSEAWG LVCPPEVMPV KTALVTSVAT VLGAGLVAAG 350
    LLLWWRKSLL YRLCPPIPRL RLPLAGEMVV WEPALEDCEV TPVTDA 396
    Length:396
    Mass (Da):43,195
    Last modified:October 1, 1993 - v1
    Checksum:iF1A5BF11743AB8A3
    GO
    Isoform 2 (identifier: P26952-3) [UniParc]FASTAAdd to Basket

    Also known as: SP2

    The sequence of this isoform differs from the canonical sequence as follows:
         20-112: Missing.

    Show »
    Length:303
    Mass (Da):33,203
    Checksum:i044AE7DB3E1E2587
    GO
    Isoform 3 (identifier: P26952-2) [UniParc]FASTAAdd to Basket

    Also known as: A

    The sequence of this isoform differs from the canonical sequence as follows:
         274-283: Missing.

    Show »
    Length:386
    Mass (Da):42,017
    Checksum:i9E383407D88AD774
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti113 – 1131D → G no nucleotide entry (PubMed:7889941)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei20 – 11293Missing in isoform 2. 1 PublicationVSP_040623Add
    BLAST
    Alternative sequencei274 – 28310Missing in isoform 3. 1 PublicationVSP_011265

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64534 mRNA. Translation: CAA45833.1.
    FJ550346 mRNA. Translation: ACM24115.1.
    CCDSiCCDS26827.1. [P26952-1]
    PIRiS22909.
    RefSeqiNP_032395.1. NM_008369.1. [P26952-1]
    UniGeneiMm.425857.

    Genome annotation databases

    EnsembliENSMUST00000090591; ENSMUSP00000088079; ENSMUSG00000068758. [P26952-1]
    GeneIDi16188.
    KEGGimmu:16188.
    UCSCiuc007sgm.1. mouse. [P26952-1]
    uc011zgb.1. mouse. [P26952-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64534 mRNA. Translation: CAA45833.1 .
    FJ550346 mRNA. Translation: ACM24115.1 .
    CCDSi CCDS26827.1. [P26952-1 ]
    PIRi S22909.
    RefSeqi NP_032395.1. NM_008369.1. [P26952-1 ]
    UniGenei Mm.425857.

    3D structure databases

    ProteinModelPortali P26952.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200636. 3 interactions.
    DIPi DIP-59621N.
    STRINGi 10090.ENSMUSP00000088079.

    PTM databases

    PhosphoSitei P26952.

    Proteomic databases

    PRIDEi P26952.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000090591 ; ENSMUSP00000088079 ; ENSMUSG00000068758 . [P26952-1 ]
    GeneIDi 16188.
    KEGGi mmu:16188.
    UCSCi uc007sgm.1. mouse. [P26952-1 ]
    uc011zgb.1. mouse. [P26952-3 ]

    Organism-specific databases

    CTDi 3563.
    MGIi MGI:96553. Il3ra.

    Phylogenomic databases

    eggNOGi NOG44788.
    GeneTreei ENSGT00520000055993.
    HOVERGENi HBG052115.
    InParanoidi P26952.
    KOi K04737.
    OMAi KNLRMEP.
    OrthoDBi EOG7TJ3J7.
    PhylomeDBi P26952.
    TreeFami TF331549.

    Miscellaneous databases

    NextBioi 289089.
    PROi P26952.
    SOURCEi Search...

    Gene expression databases

    Bgeei P26952.
    CleanExi MM_IL3RA.
    Genevestigatori P26952.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR003961. Fibronectin_type3.
    IPR013783. Ig-like_fold.
    IPR015321. IL-6_rcpt_alpha-bd.
    IPR003532. Short_hematopoietin_rcpt_2_CS.
    [Graphical view ]
    Pfami PF09240. IL6Ra-bind. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 1 hit.
    PROSITEi PS01356. HEMATOPO_REC_S_F2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two distinct functional high affinity receptors for mouse interleukin-3 (IL-3)."
      Hara T., Miyajima A.
      EMBO J. 11:1875-1884(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: B6.S.
    2. "Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene."
      Ichihara M., Hara T., Takagi M., Cho L.C., Gorman D.M., Miyajima A.
      EMBO J. 14:939-950(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), SUBCELLULAR LOCATION.
      Strain: A/J and C57BL/6.
    3. "A new isoform of IL-3 receptor alpha with novel differentiation activity and high affinity binding mode."
      Chen J., Olsen J., Ford S., Mirza S., Walker A., Murphy J.M., Young I.G.
      J. Biol. Chem. 284:5763-5773(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.

    Entry informationi

    Entry nameiIL3RA_MOUSE
    AccessioniPrimary (citable) accession number: P26952
    Secondary accession number(s): B9VI80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3