P26940 (TRPE_METTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 1
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	MTBMA_c02340

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Anthranilate synthase component 1		PRO_0000154130
Experimental info
	Sequence conflict	7	1	G → K in AAA73028. Ref.1
	Sequence conflict	297	1	E → EQ in AAA73028. Ref.1
	Sequence conflict	459	1	D → Y in AAA73028. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P26940 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 4A90AD4B91846E2A	FASTA	461	51,911
	10 20 30 40 50 60 MGVNVFGIIN LEKPRKEKLE FREPFEVFKS IYSEYESSFL LESMESDTGL ARYSFIGFEP 70 80 90 100 110 120 EMIIRAREGV IEIDDGDSRE EFDSKNPFED LRGFLKMEKN SGGFCGGLVG YISYQAARFF 130 140 150 160 170 180 DTIRLSEGDF PDFEFGLFLD GIMFNHLTGE CSYISRHGNR LPDISPLLGD EVPTGTLGYR 190 200 210 220 230 240 RERTLLSKRR YMDMVLEAKE RIREGEIFQA VLSSATDYRL RGDRLAFYES LRRINPSPYM 250 260 270 280 290 300 YHLKLGEREI TGSSPEMLVR VEDRRIETFP IAGTRPRGRT EEEDGVIASD LLSDEKELAE 310 320 330 340 350 360 HLMLVDLARN DIGRVSEFGS VEVPEYMTIK RFSHVQHILS HVTGKLRDGM DAVDALGAVF 370 380 390 400 410 420 PAGTVSGAPK IRAMEIIESL EGVPRNAYAG ALGYLSLNGN ADFAITIRSM VCQGKTGRIQ 430 440 450 460 AGAGIVHDSI PEMEYLECQN KARALLKSME MAGEQVDPDN R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tryptophan gene cluster of Methanobacterium thermoautotrophicum Marburg: molecular cloning and nucleotide sequence of a putative trpEGCFBAD operon." Meile L., Stettler R., Banholzer R., Kotik M., Leisinger T. J. Bacteriol. 173:5017-5023(1991) [PubMed: 1860817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed: 20802048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M65060 Genomic DNA. Translation: AAA73028.1. CP001710 Genomic DNA. Translation: ADL57843.1.
RefSeq	YP_003849156.1. NC_014408.1.
3D structure databases
ProteinModelPortal	P26940.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9703940.
GenomeReviews	Gene locus MTBMA_c02340 in contig CP001710_GR.
KEGG	mmg:MTBMA_c02340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VRSLMDI.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR019999. Anth_synth_I. IPR006805. Anth_synth_I_N. IPR010116. Anthranilate_synth_I_arc. IPR015890. Chorismate-bd_C. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
KO	K01657.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMs	TIGR01820. TrpE-arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_METTM

Accession

Primary (citable) accession number: P26940
Secondary accession number(s): D9PUE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 30, 2010
Last modified:	November 16, 2011
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program