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Protein

Calponin-1

Gene

CNN1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calponin-1
Alternative name(s):
Calponin, smooth muscle
Gene namesi
Name:CNN1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002047711 – 292Calponin-1Add BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170Phosphothreonine; by ROCK21 Publication1
Modified residuei175Phosphoserine; by PKC, CaMK2 and ROCK22 Publications1
Modified residuei180Phosphothreonine; by ROCK21 Publication1
Modified residuei184Phosphothreonine; by PKC and CaMK22 Publications1
Modified residuei184Phosphothreonine; by ROCK22 Publications1
Modified residuei259Phosphothreonine; by ROCK21 Publication1

Post-translational modificationi

Phosphorylation by PKC or CaM kinase II reduces the binding of calponin to F-actin and tropomyosin.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP26932.

PTM databases

iPTMnetiP26932.

Expressioni

Tissue specificityi

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTA1P681355EBI-8602797,EBI-367540From a different organism.

Protein-protein interaction databases

IntActiP26932. 1 interactor.
MINTiMINT-2880197.

Structurei

Secondary structure

1292
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 42Combined sources11
Helixi50 – 56Combined sources7
Helixi59 – 67Combined sources9
Helixi82 – 98Combined sources17
Helixi102 – 104Combined sources3
Helixi108 – 113Combined sources6
Helixi118 – 133Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H67NMR-A28-134[»]
ProteinModelPortaliP26932.
SMRiP26932.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26932.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 131CHPROSITE-ProRule annotationAdd BLAST104
Repeati164 – 189Calponin-like 1Add BLAST26
Repeati204 – 229Calponin-like 2Add BLAST26
Repeati243 – 268Calponin-like 3Add BLAST26

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 193Calmodulin-bindingCurated9

Sequence similaritiesi

Belongs to the calponin family.Curated
Contains 3 calponin-like repeats.PROSITE-ProRule annotation
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074447.
HOVERGENiHBG005186.
InParanoidiP26932.
PhylomeDBiP26932.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: P26932-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSNANFNRGP AYGLSAEVKN KLAQKYDPQT ERQLRVWIEG ATGRRIGDNF
60 70 80 90 100
MDGLKDGVIL CELINKLQPG SVQKVNDPVQ NWHKLENIGN FLRAIKHYGV
110 120 130 140 150
KPHDIFEAND LFENTNHTQV QSTLIALASQ AKTKGNNVGL GVKYAEKQQR
160 170 180 190 200
RFQPEKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD
210 220 230 240 250
QATISLQMGT NKGASQAGMT APGTKRQIFE PSLGMERCDT NIIGLQMGSN
260 270 280 290
KGASQQGMTV YGLPRQVYDP KYCDAPGLLG EDGLNHSFYN SQ
Length:292
Mass (Da):32,361
Last modified:September 19, 2003 - v2
Checksum:iA80E55FE5966DAC6
GO
Isoform Beta (identifier: P26932-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-256: Missing.

Show »
Length:252
Mass (Da):28,154
Checksum:i10E36CD5D211DB0B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66K → T in AAA48651 (PubMed:2071603).Curated1
Sequence conflicti66K → T in AAA48652 (PubMed:2071603).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000756217 – 256Missing in isoform Beta. CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63559 mRNA. Translation: AAA48651.1.
M63560 mRNA. Translation: AAA48652.1.
PIRiA39871.
RefSeqiNP_990847.1. NM_205516.1.
UniGeneiGga.873.

Genome annotation databases

EnsembliENSGALT00000046726; ENSGALP00000046676; ENSGALG00000041266. [P26932-1]
GeneIDi396522.
KEGGigga:396522.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63559 mRNA. Translation: AAA48651.1.
M63560 mRNA. Translation: AAA48652.1.
PIRiA39871.
RefSeqiNP_990847.1. NM_205516.1.
UniGeneiGga.873.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H67NMR-A28-134[»]
ProteinModelPortaliP26932.
SMRiP26932.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP26932. 1 interactor.
MINTiMINT-2880197.

PTM databases

iPTMnetiP26932.

Proteomic databases

PRIDEiP26932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000046726; ENSGALP00000046676; ENSGALG00000041266. [P26932-1]
GeneIDi396522.
KEGGigga:396522.

Organism-specific databases

CTDi1264.

Phylogenomic databases

GeneTreeiENSGT00550000074447.
HOVERGENiHBG005186.
InParanoidiP26932.
PhylomeDBiP26932.

Miscellaneous databases

EvolutionaryTraceiP26932.
PROiP26932.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNN1_CHICK
AccessioniPrimary (citable) accession number: P26932
Secondary accession number(s): P26933, Q9PSG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: September 19, 2003
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.