P26922 (TRPG_AZOBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 2
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component II
Glutamine amido-transferase

Gene names

Name:

trpG

Organism

Azospirillum brasilense

Taxonomic identifier

192 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Participates in the tryptophan-dependent indole-3-acetic acid production, which is a phytohormone released by A.brasilense.
Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Contains 2 chains with different activities: component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Anthranilate synthase component 2		PRO_0000056871
Regions
	Domain	1 – 195	195	Glutamine amidotransferase type-1
Sites
	Active site	80	1	By similarity
	Active site	169	1	By similarity
	Active site	171	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26922 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: BBE41EEB84CC0EAA	FASTA	196	21,405
	10 20 30 40 50 60 MLLLIDNYDS FTYNLVHYLG ELGAELDVRR NDSLTVEEAM ALRPEGIVLS PGPCDPDKAG 70 80 90 100 110 120 ICLPLIDAAA KAAVPLMGVC LGHQAIGQPF GGTVVRAPVP MHGKVDRMFH QGRGVLKDLP 130 140 150 160 170 180 SPFRATRYHS LIVERATLPA CLEVTGETED GLIMALSHRE LPIHGVQFHP ESIESEHGHK 190 ILENFLNTTR RLETAA

References

[1]	"Relationship between tryptophan biosynthesis and indole-3-acetic acid production in Azospirillum: identification and sequencing of a trpGDC cluster." Zimmer W., Aparicio C., Elmerich C. Mol. Gen. Genet. 229:41-51(1991) [PubMed: 1896020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29145 / Sp7 / DSM 1690 / IMET 11303.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57853 Genomic DNA. Translation: CAA40984.1.
PIR	S17703.
3D structure databases
ProteinModelPortal	P26922.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR006221. TrpG_papA. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00566. TrpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPG_AZOBR

Accession

Primary (citable) accession number: P26922

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program