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P26919

- T2A1_AZOBR

UniProt

P26919 - T2A1_AZOBR

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Protein

Type-2 restriction enzyme AbrI

Gene

abrIR

Organism
Azospirillum brasilense
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi8. AbrI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme AbrI (EC:3.1.21.4)
Short name:
R.AbrI
Alternative name(s):
Endonuclease AbrI
Type II restriction enzyme AbrI
Gene namesi
Name:abrIR
OrganismiAzospirillum brasilense
Taxonomic identifieri192 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeAzospirillum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Type-2 restriction enzyme AbrIPRO_0000077277Add
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 477Arg/Lys-rich (basic)

Sequence similaritiesi

Family and domain databases

InterProiIPR007636. Restrct_endonuc_II_XhoI.
[Graphical view]
PfamiPF04555. XhoI. 1 hit.
[Graphical view]
PIRSFiPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.

Sequencei

Sequence statusi: Complete.

P26919-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALELADYER KARESVMAFW GNREKARQKQ QESGKPDQGE RRRDRRKNMD
60 70 80 90 100
GFCALVIDII RANGLHRAEI HQKRALLTLP GYFRPTKLWD LLVIQDRRLV
110 120 130 140 150
AALEFKSQVG PSFGNNFNNR TEEAIGTAHD LWTAYREGAF GQSPRPFVGW
160 170 180 190 200
LMLVEDAPES NQRRADTSPH FRCSRSSRAH PTYGGMTSSA SGSSRSSFTP
210 220
RPRSSPRRAP PPRQGRIPSP RI
Length:222
Mass (Da):25,260
Last modified:August 1, 1992 - v1
Checksum:i8FB124EFC4BBDCC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62690 Genomic DNA. Translation: CAA44567.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62690 Genomic DNA. Translation: CAA44567.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 8. AbrI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007636. Restrct_endonuc_II_XhoI.
[Graphical view ]
Pfami PF04555. XhoI. 1 hit.
[Graphical view ]
PIRSFi PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "AbrI restriction modification system from Azospirillium brasilense, molecular cloning and characterization of its genes."
    Schwabe G., Helke A., Klingmueller W.
    Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29711 / DSM 1844.

Entry informationi

Entry nameiT2A1_AZOBR
AccessioniPrimary (citable) accession number: P26919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3