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P26919 (T2A1_AZOBR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme AbrI

Short name=R.AbrI
EC=3.1.21.4
Alternative name(s):
Endonuclease AbrI
Type II restriction enzyme AbrI
Gene names
Name:abrIR
OrganismAzospirillum brasilense
Taxonomic identifier192 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeAzospirillum

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Sequence similarities

Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222Type-2 restriction enzyme AbrI
PRO_0000077277

Regions

Compositional bias41 – 477Arg/Lys-rich (basic)

Sequences

Sequence LengthMass (Da)Tools
P26919 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 8FB124EFC4BBDCC1

FASTA22225,260
        10         20         30         40         50         60 
MALELADYER KARESVMAFW GNREKARQKQ QESGKPDQGE RRRDRRKNMD GFCALVIDII 

        70         80         90        100        110        120 
RANGLHRAEI HQKRALLTLP GYFRPTKLWD LLVIQDRRLV AALEFKSQVG PSFGNNFNNR 

       130        140        150        160        170        180 
TEEAIGTAHD LWTAYREGAF GQSPRPFVGW LMLVEDAPES NQRRADTSPH FRCSRSSRAH 

       190        200        210        220 
PTYGGMTSSA SGSSRSSFTP RPRSSPRRAP PPRQGRIPSP RI 

« Hide

References

[1]"AbrI restriction modification system from Azospirillium brasilense, molecular cloning and characterization of its genes."
Schwabe G., Helke A., Klingmueller W.
Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29711 / DSM 1844.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62690 Genomic DNA. Translation: CAA44567.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE8. AbrI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007636. Restrct_endonuc_II_XhoI.
[Graphical view]
PfamPF04555. XhoI. 1 hit.
[Graphical view]
PIRSFPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2A1_AZOBR
AccessionPrimary (citable) accession number: P26919
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries