P26919 (T2A1_AZOBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme AbrI
Short name=R.AbrI
EC=3.1.21.4

Alternative name(s):
Endonuclease AbrI
Type II restriction enzyme AbrI

Gene names

Name:

abrIR

Organism

Azospirillum brasilense

Taxonomic identifier

192 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Sequence similarities	Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Type-2 restriction enzyme AbrI		PRO_0000077277
Regions
	Compositional bias	41 – 47	7	Arg/Lys-rich (basic)

Sequences

	Sequence		Length	Mass (Da)
	P26919 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 8FB124EFC4BBDCC1	FASTA	222	25,260
	10 20 30 40 50 60 MALELADYER KARESVMAFW GNREKARQKQ QESGKPDQGE RRRDRRKNMD GFCALVIDII 70 80 90 100 110 120 RANGLHRAEI HQKRALLTLP GYFRPTKLWD LLVIQDRRLV AALEFKSQVG PSFGNNFNNR 130 140 150 160 170 180 TEEAIGTAHD LWTAYREGAF GQSPRPFVGW LMLVEDAPES NQRRADTSPH FRCSRSSRAH 190 200 210 220 PTYGGMTSSA SGSSRSSFTP RPRSSPRRAP PPRQGRIPSP RI

References

[1]	"AbrI restriction modification system from Azospirillium brasilense, molecular cloning and characterization of its genes." Schwabe G., Helke A., Klingmueller W. Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29711 / DSM 1844.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62690 Genomic DNA. Translation: CAA44567.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	8. AbrI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007636. Restrct_endonuc_II_XhoI. [Graphical view]
Pfam	PF04555. XhoI. 1 hit. [Graphical view]
PIRSF	PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T2A1_AZOBR

Accession

Primary (citable) accession number: P26919

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	July 11, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program