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P26919

- T2A1_AZOBR

UniProt

P26919 - T2A1_AZOBR

Protein

Type-2 restriction enzyme AbrI

Gene

abrIR

Organism
Azospirillum brasilense
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi8. AbrI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme AbrI (EC:3.1.21.4)
    Short name:
    R.AbrI
    Alternative name(s):
    Endonuclease AbrI
    Type II restriction enzyme AbrI
    Gene namesi
    Name:abrIR
    OrganismiAzospirillum brasilense
    Taxonomic identifieri192 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeAzospirillum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 222222Type-2 restriction enzyme AbrIPRO_0000077277Add
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi41 – 477Arg/Lys-rich (basic)

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR007636. Restrct_endonuc_II_XhoI.
    [Graphical view]
    PfamiPF04555. XhoI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P26919-1 [UniParc]FASTAAdd to Basket

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    MALELADYER KARESVMAFW GNREKARQKQ QESGKPDQGE RRRDRRKNMD    50
    GFCALVIDII RANGLHRAEI HQKRALLTLP GYFRPTKLWD LLVIQDRRLV 100
    AALEFKSQVG PSFGNNFNNR TEEAIGTAHD LWTAYREGAF GQSPRPFVGW 150
    LMLVEDAPES NQRRADTSPH FRCSRSSRAH PTYGGMTSSA SGSSRSSFTP 200
    RPRSSPRRAP PPRQGRIPSP RI 222
    Length:222
    Mass (Da):25,260
    Last modified:August 1, 1992 - v1
    Checksum:i8FB124EFC4BBDCC1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62690 Genomic DNA. Translation: CAA44567.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62690 Genomic DNA. Translation: CAA44567.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 8. AbrI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007636. Restrct_endonuc_II_XhoI.
    [Graphical view ]
    Pfami PF04555. XhoI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "AbrI restriction modification system from Azospirillium brasilense, molecular cloning and characterization of its genes."
      Schwabe G., Helke A., Klingmueller W.
      Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 29711 / DSM 1844.

    Entry informationi

    Entry nameiT2A1_AZOBR
    AccessioniPrimary (citable) accession number: P26919
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3