Reviewed, UniProtKB/Swiss-Prot P26918 (BLAB_AERHY)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6

Gene names

Name:

cphA

Organism

Aeromonas hydrophila

Taxonomic identifier

644 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can hydrolyze carbapenem compounds.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Periplasm Probable.
Induction	By beta-lactam antibiotics.
Sequence similarities	Belongs to the class-B beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Periplasm
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Potential

Chain

28 – 254

227

Beta-lactamase

PRO_0000016941

Sites

Metal binding

95

1

Zinc 1 By similarity

Metal binding

97

1

Zinc 1 By similarity

Metal binding

99

1

Zinc 2 By similarity

Metal binding

174

1

Zinc 1 By similarity

Metal binding

193

1

Zinc 2 By similarity

Metal binding

231

1

Zinc 2 By similarity

Secondary structure

1

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254

Helix Strand Turn

Details...

Beta strand

30 – 36

7

Beta strand

39 – 44

6

Beta strand

46 – 48

3

Beta strand

50 – 56

7

Beta strand

61 – 65

5

Helix

70 – 81

12

Beta strand

88 – 92

5

Beta strand

94 – 97

4

Helix

98 – 101

4

Helix

104 – 109

6

Beta strand

113 – 117

5

Helix

118 – 138

21

Beta strand

151 – 156

6

Beta strand

158 – 160

3

Turn

161 – 164

4

Beta strand

165 – 168

4

Beta strand

173 – 177

5

Beta strand

180 – 183

4

Turn

184 – 187

4

Beta strand

188 – 190

3

Helix

192 – 194

3

Helix

209 – 218

10

Beta strand

225 – 228

4

Beta strand

230 – 233

4

Helix

239 – 249

11

Sequences

	Sequence		Length	Mass (Da)
	P26918-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 9D54760E17CF4B50	FASTA	254	28,016
	10 20 30 40 50 60 MMKGWMKCGL AGAVVLMASF WGGSVRAAGM SLTQVSGPVY VVEDNYYVQE NSMVYFGAKG 70 80 90 100 110 120 VTVVGATWTP DTARELHKLI KRVSRKPVLE VINTNYHTDR AGGNAYWKSI GAKVVSTRQT 130 140 150 160 170 180 RDLMKSDWAE IVAFTRKGLP EYPDLPLVLP NVVHDGDFTL QEGKVRAFYA GPAHTPDGIF 190 200 210 220 230 240 VYFPDEQVLY GNCILKEKLG NLSFADVKAY PQTLERLKAM KLPIKTVIGG HDSPLHGPEL 250 IDHYEALIKA APQS

References

[1]	"The Aeromonas hydrophila cphA gene: molecular heterogeneity among class B metallo-beta-lactamases." Massidda O., Rossolini G.M., Satta G. J. Bacteriol. 173:4611-4617(1991) [PubMed: 1856163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AE036.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X57102 Genomic DNA. Translation: CAA40386.1.

PIR

S17287.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X8G	X-ray	1.70	A	28-254	[»]
1X8H	X-ray	1.60	A	28-254	[»]
1X8I	X-ray	1.90	A	28-254	[»]
2GKL	X-ray	1.86	A	28-254	[»]
2QDS	X-ray	1.66	A	28-254	[»]
3F9O	X-ray	2.03	A	28-254	[»]
3FAI	X-ray	1.70	A	28-254	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.5.2.6. 3060.

Family and domain databases

InterPro

IPR001018. Beta-lactamase_class-B_CS.
IPR001279. Blactmase-like.
[Graphical view]

Pfam

PF00753. Lactamase_B. 1 hit.
[Graphical view]

PROSITE

PS00743. BETA_LACTAMASE_B_1. 1 hit.
PS00744. BETA_LACTAMASE_B_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BLAB_AERHY

Accession

Primary (citable) accession number: P26918

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)