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P26918

- BLAB_AERHY

UniProt

P26918 - BLAB_AERHY

Protein

Beta-lactamase

Gene

cphA

Organism
Aeromonas hydrophila
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Can hydrolyze carbapenem compounds.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi95 – 951Zinc 1By similarity
    Metal bindingi97 – 971Zinc 1By similarity
    Metal bindingi99 – 991Zinc 2By similarity
    Metal bindingi174 – 1741Zinc 1By similarity
    Metal bindingi193 – 1931Zinc 2By similarity
    Metal bindingi231 – 2311Zinc 2By similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP26918.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase (EC:3.5.2.6)
    Gene namesi
    Name:cphA
    OrganismiAeromonas hydrophila
    Taxonomic identifieri644 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

    Subcellular locationi

    Periplasm Curated

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 254227Beta-lactamasePRO_0000016941Add
    BLAST

    Expressioni

    Inductioni

    By beta-lactam antibiotics.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    254
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 367
    Beta strandi39 – 446
    Beta strandi46 – 483
    Beta strandi50 – 567
    Beta strandi61 – 655
    Helixi70 – 8112
    Beta strandi88 – 925
    Beta strandi94 – 974
    Helixi98 – 1014
    Helixi104 – 1096
    Beta strandi113 – 1175
    Helixi118 – 13821
    Beta strandi151 – 1566
    Beta strandi158 – 1603
    Turni161 – 1644
    Beta strandi165 – 1684
    Beta strandi173 – 1775
    Beta strandi180 – 1834
    Turni184 – 1874
    Beta strandi188 – 1903
    Helixi192 – 1943
    Beta strandi198 – 2003
    Helixi209 – 21911
    Beta strandi225 – 2284
    Beta strandi230 – 2323
    Helixi239 – 25012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1X8GX-ray1.70A28-254[»]
    1X8HX-ray1.60A28-254[»]
    1X8IX-ray1.90A28-254[»]
    2GKLX-ray1.86A28-254[»]
    2QDSX-ray1.66A28-254[»]
    3F9OX-ray2.03A28-254[»]
    3FAIX-ray1.70A28-254[»]
    3IOFX-ray1.44A28-254[»]
    3IOGX-ray1.41A28-254[»]
    3SW3X-ray2.35A28-254[»]
    3T9MX-ray2.03A28-254[»]
    ProteinModelPortaliP26918.
    SMRiP26918. Positions 28-251.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26918.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiIPR001279. Beta-lactamas-like.
    IPR001018. Beta-lactamase_class-B_CS.
    [Graphical view]
    PfamiPF00753. Lactamase_B. 1 hit.
    [Graphical view]
    SMARTiSM00849. Lactamase_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF56281. SSF56281. 1 hit.
    PROSITEiPS00743. BETA_LACTAMASE_B_1. 1 hit.
    PS00744. BETA_LACTAMASE_B_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26918-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMKGWMKCGL AGAVVLMASF WGGSVRAAGM SLTQVSGPVY VVEDNYYVQE    50
    NSMVYFGAKG VTVVGATWTP DTARELHKLI KRVSRKPVLE VINTNYHTDR 100
    AGGNAYWKSI GAKVVSTRQT RDLMKSDWAE IVAFTRKGLP EYPDLPLVLP 150
    NVVHDGDFTL QEGKVRAFYA GPAHTPDGIF VYFPDEQVLY GNCILKEKLG 200
    NLSFADVKAY PQTLERLKAM KLPIKTVIGG HDSPLHGPEL IDHYEALIKA 250
    APQS 254
    Length:254
    Mass (Da):28,016
    Last modified:August 1, 1992 - v1
    Checksum:i9D54760E17CF4B50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57102 Genomic DNA. Translation: CAA40386.1.
    PIRiS17287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57102 Genomic DNA. Translation: CAA40386.1 .
    PIRi S17287.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1X8G X-ray 1.70 A 28-254 [» ]
    1X8H X-ray 1.60 A 28-254 [» ]
    1X8I X-ray 1.90 A 28-254 [» ]
    2GKL X-ray 1.86 A 28-254 [» ]
    2QDS X-ray 1.66 A 28-254 [» ]
    3F9O X-ray 2.03 A 28-254 [» ]
    3FAI X-ray 1.70 A 28-254 [» ]
    3IOF X-ray 1.44 A 28-254 [» ]
    3IOG X-ray 1.41 A 28-254 [» ]
    3SW3 X-ray 2.35 A 28-254 [» ]
    3T9M X-ray 2.03 A 28-254 [» ]
    ProteinModelPortali P26918.
    SMRi P26918. Positions 28-251.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P26918.
    ChEMBLi CHEMBL1169593.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P26918.

    Miscellaneous databases

    EvolutionaryTracei P26918.

    Family and domain databases

    Gene3Di 3.60.15.10. 1 hit.
    InterProi IPR001279. Beta-lactamas-like.
    IPR001018. Beta-lactamase_class-B_CS.
    [Graphical view ]
    Pfami PF00753. Lactamase_B. 1 hit.
    [Graphical view ]
    SMARTi SM00849. Lactamase_B. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56281. SSF56281. 1 hit.
    PROSITEi PS00743. BETA_LACTAMASE_B_1. 1 hit.
    PS00744. BETA_LACTAMASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Aeromonas hydrophila cphA gene: molecular heterogeneity among class B metallo-beta-lactamases."
      Massidda O., Rossolini G.M., Satta G.
      J. Bacteriol. 173:4611-4617(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AE036.

    Entry informationi

    Entry nameiBLAB_AERHY
    AccessioniPrimary (citable) accession number: P26918
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3