P26900 (ASPG1_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-asparaginase 1
Short name=L-ASNase 1
EC=3.5.1.1

Alternative name(s):
L-asparagine amidohydrolase 1

Gene names

Name:	ansA
Ordered Locus Names:	BSU23580

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Induction	Expression is induced by asparagine. Ref.5
Miscellaneous	B.subtilis contains two L-asparaginase isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme.
Sequence similarities	Belongs to the asparaginase 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative initiation
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: P26900-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced in 2 x SG medium but not in 2 x YT medium.

Isoform Short (identifier: P26900-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: Missing.

Note: Produced in 2 x YT medium but not in 2 x SG medium.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	L-asparaginase 1		PRO_0000002354
Regions
	Region	85 – 86	2	Substrate binding By similarity
Sites
	Active site	12	1	O-isoaspartyl threonine intermediate By similarity
	Binding site	54	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 5	5	Missing in isoform Short.		VSP_018651

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 4977F09E58CAB6C4	FASTA	329	36,455
	10 20 30 40 50 60 MKKLLMLTTG GTIASVEGEN GLAPGVKADE LLSYVSKLDN DYTMETQSLM NIDSTNMQPE 70 80 90 100 110 120 YWVEIAEAVK ENYDAYDGFV ITHGTDTMAY TSAALSYMLQ HAKKPIVITG SQIPITFQKT 130 140 150 160 170 180 DAKKNITDAI RFACEGVGGV YVVFDGRVIQ GTRAIKLRTK SYDAFESINY PYIAFINEDG 190 200 210 220 230 240 IEYNKQVTEP ENDTFTVDTS LCTDVCLLKL HPGLKPEMFD ALKSMYKGIV IESYGSGGVP 250 260 270 280 290 300 FEGRDILSKV NELIESGIVV VITTQCLEEG EDMSIYEVGR RVNQDLIIRS RNMNTEAIVP 310 320 KLMWALGQSS DLPVVKRIME TPIADDVVL
	Isoform Short [UniParc]. Checksum: 7A273E8FED4C19AF	FASTA	324	35,841
	10 20 30 40 50 60 MLTTGGTIAS VEGENGLAPG VKADELLSYV SKLDNDYTME TQSLMNIDST NMQPEYWVEI 70 80 90 100 110 120 AEAVKENYDA YDGFVITHGT DTMAYTSAAL SYMLQHAKKP IVITGSQIPI TFQKTDAKKN 130 140 150 160 170 180 ITDAIRFACE GVGGVYVVFD GRVIQGTRAI KLRTKSYDAF ESINYPYIAF INEDGIEYNK 190 200 210 220 230 240 QVTEPENDTF TVDTSLCTDV CLLKLHPGLK PEMFDALKSM YKGIVIESYG SGGVPFEGRD 250 260 270 280 290 300 ILSKVNELIE SGIVVVITTQ CLEEGEDMSI YEVGRRVNQD LIIRSRNMNT EAIVPKLMWA 310 320 LGQSSDLPVV KRIMETPIAD DVVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase." Sun D., Setlow P. J. Bacteriol. 173:3831-3845(1991) [PubMed: 1711029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase." Sun D., Setlow P. J. Bacteriol. 175:2501-2506(1993) [PubMed: 8478318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
[5]	"Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase." Fisher S.H., Wray L.V. Jr. J. Bacteriol. 184:2148-2154(2002) [PubMed: 11914346] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63264 Genomic DNA. Translation: AAA22243.1. D84432 Genomic DNA. Translation: BAA12642.1. AL009126 Genomic DNA. Translation: CAB14290.1. L08205 Genomic DNA. No translation available.
PIR	A39440.
RefSeq	NP_390239.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P26900.
SMR	P26900. Positions 3-328.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	938722.
GenomeReviews	Gene locus BSU23580 in contig AL009126_GR.
KEGG	bsu:BSU23580.
NMPDR	fig|224308.1.peg.2363.
PATRIC	18976537. VBIBacSub10457_2459.
Organism-specific databases
GenoList	BSU23580. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000000510.
HOGENOM	HBG497495.
OMA	GTIGMQA.
PhylomeDB	P26900.
ProtClustDB	CLSK887522.
Enzyme and pathway databases
BioCyc	BSUB:BSU23580-MONOMER.
Family and domain databases
InterPro	IPR006033. AsnASEI. IPR006034. Asparaginase/glutaminase. IPR020827. Asparaginase/glutaminase_CS. [Graphical view]
KO	K01424.
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PIRSF	PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTS	PR00139. ASNGLNASE.
SMART	SM00870. Asparaginase. 1 hit. [Graphical view]
SUPFAM	SSF53774. Asp/Glutamnse. 1 hit.
TIGRFAMs	TIGR00519. AsnASE_I. 1 hit.
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG1_BACSU

Accession

Primary (citable) accession number: P26900

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program