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P26899 (ASPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartate ammonia-lyase
Short name=Aspartase
EC=4.3.1.1
Gene names
Name:ansB
Ordered Locus Names:BSU23570
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate = fumarate + NH3.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Aspartase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475Aspartate ammonia-lyase
PRO_0000161336

Regions

Region143 – 1453Substrate binding By similarity

Sites

Binding site1041Substrate By similarity

Experimental info

Sequence conflict1441T → Q in AAA22244. Ref.1
Sequence conflict1441T → Q in BAA12643. Ref.2
Sequence conflict1821D → H in AAA22244. Ref.1
Sequence conflict1821D → H in BAA12643. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P26899 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: F3E847DC83A81460

FASTA47552,504
        10         20         30         40         50         60 
MLNGQKEYRV EKDFLGEKQI EADVYYGIQT LRASENFPIT GYKIHEEMIN ALAIVKKAAA 

        70         80         90        100        110        120 
LANMDVKRLY EGIGQAIVQA ADEILEGKWH DQFIVDPIQG GAGTSMNMNA NEVIGNRALE 

       130        140        150        160        170        180 
IMGHKKGDYI HLSPNTHVNM SQSTNDVFPT AIHISTLKLL EKLLKTMEDM HSVFKQKAQE 

       190        200        210        220        230        240 
FDSVIKMGRT HLQDAVPIRL GQEFEAYSRV LERDIKRIKQ SRQHLYEVNM GATAVGTGLN 

       250        260        270        280        290        300 
ADPEYIKQVV KHLADISGLP LVGADHLVDA TQNTDAYTEV SASLKVCMMN MSKIANDLRL 

       310        320        330        340        350        360 
MASGPRAGLA EISLPARQPG SSIMPGKVNP VMAELINQIA FQVIGNDNTI CLASEAGQLE 

       370        380        390        400        410        420 
LNVMEPVLVF NLLQSISIMN NGFRSFTDNC LKGIEANEKR MKQYVEKSAG VITAVNPHLG 

       430        440        450        460        470 
YEAAARIARE AIMTGQSVRD LCLQHDVLTE EELDIILNPY EMTKPGIAGK ELLEK

« Hide

References

« Hide 'large scale' references
[1]"Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase."
Sun D., Setlow P.
J. Bacteriol. 173:3831-3845(1991) [PubMed: 1711029] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 144 AND 182.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M63264 Genomic DNA. Translation: AAA22244.1.
D84432 Genomic DNA. Translation: BAA12643.1.
AL009126 Genomic DNA. Translation: CAB14289.2.
PIRUFBSD. B39440.
RefSeqNP_390238.2. NC_000964.3.

3D structure databases

ProteinModelPortalP26899.
SMRP26899. Positions 9-469.
ModBaseSearch...

Protein-protein interaction databases

IntActP26899. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000000486; EBBACP00000000486; EBBACG00000000484.
GeneID938721.
GenomeReviewsGene locus BSU23570 in contig AL009126_GR.
KEGGbsu:BSU23570.
NMPDRfig|224308.1.peg.2362.
PATRIC18976535. VBIBacSub10457_2458.

Organism-specific databases

GenoListBSU23570. [Micado]

Phylogenomic databases

GeneTreeEBGT00070000031773.
HOGENOMHBG284369.
PhylomeDBP26899.
ProtClustDBPRK12273.

Enzyme and pathway databases

BioCycBSUB:BSU23570-MONOMER.

Family and domain databases

InterProIPR004708. ApsA.
IPR003031. D_crystallin.
IPR005677. Fum_hydII.
IPR018951. Fumarase_C_C.
IPR000362. Fumarate_lyase.
IPR020557. Fumarate_lyase_CS.
IPR008948. L-Aspartase-like.
IPR024083. L-Aspartase-like_N.
IPR022761. Lyase1_N.
[Graphical view]
Gene3DG3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KOK01744.
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00145. ARGSUCLYASE.
PR00149. FUMRATELYASE.
SUPFAMSSF48557. L-Aspartase-like. 1 hit.
TIGRFAMsTIGR00839. AspA. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASPA_BACSU
AccessionPrimary (citable) accession number: P26899
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 7, 2009
Last modified: January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

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