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Protein

Aspartate ammonia-lyase

Gene

ansB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-aspartate = fumarate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Enzyme and pathway databases

BioCyciBSUB:BSU23570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate ammonia-lyase (EC:4.3.1.1)
Short name:
Aspartase
Gene namesi
Name:ansB
Ordered Locus Names:BSU23570
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001613361 – 475Aspartate ammonia-lyaseAdd BLAST475

Proteomic databases

PaxDbiP26899.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiP26899. 2 interactors.
STRINGi224308.Bsubs1_010100012936.

Structurei

3D structure databases

ProteinModelPortaliP26899.
SMRiP26899.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni143 – 145Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108IJ0. Bacteria.
COG1027. LUCA.
HOGENOMiHOG000061737.
InParanoidiP26899.
KOiK01744.
OMAiMMHSALK.
PhylomeDBiP26899.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR004708. ApsA.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00839. aspA. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26899-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNGQKEYRV EKDFLGEKQI EADVYYGIQT LRASENFPIT GYKIHEEMIN
60 70 80 90 100
ALAIVKKAAA LANMDVKRLY EGIGQAIVQA ADEILEGKWH DQFIVDPIQG
110 120 130 140 150
GAGTSMNMNA NEVIGNRALE IMGHKKGDYI HLSPNTHVNM SQSTNDVFPT
160 170 180 190 200
AIHISTLKLL EKLLKTMEDM HSVFKQKAQE FDSVIKMGRT HLQDAVPIRL
210 220 230 240 250
GQEFEAYSRV LERDIKRIKQ SRQHLYEVNM GATAVGTGLN ADPEYIKQVV
260 270 280 290 300
KHLADISGLP LVGADHLVDA TQNTDAYTEV SASLKVCMMN MSKIANDLRL
310 320 330 340 350
MASGPRAGLA EISLPARQPG SSIMPGKVNP VMAELINQIA FQVIGNDNTI
360 370 380 390 400
CLASEAGQLE LNVMEPVLVF NLLQSISIMN NGFRSFTDNC LKGIEANEKR
410 420 430 440 450
MKQYVEKSAG VITAVNPHLG YEAAARIARE AIMTGQSVRD LCLQHDVLTE
460 470
EELDIILNPY EMTKPGIAGK ELLEK
Length:475
Mass (Da):52,504
Last modified:July 7, 2009 - v2
Checksum:iF3E847DC83A81460
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144T → Q in AAA22244 (PubMed:1711029).Curated1
Sequence conflicti144T → Q in BAA12643 (PubMed:8969508).Curated1
Sequence conflicti182D → H in AAA22244 (PubMed:1711029).Curated1
Sequence conflicti182D → H in BAA12643 (PubMed:8969508).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63264 Genomic DNA. Translation: AAA22244.1.
D84432 Genomic DNA. Translation: BAA12643.1.
AL009126 Genomic DNA. Translation: CAB14289.2.
PIRiB39440. UFBSD.
RefSeqiNP_390238.2. NC_000964.3.
WP_003230430.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14289; CAB14289; BSU23570.
GeneIDi938721.
KEGGibsu:BSU23570.
PATRICi18976535. VBIBacSub10457_2458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63264 Genomic DNA. Translation: AAA22244.1.
D84432 Genomic DNA. Translation: BAA12643.1.
AL009126 Genomic DNA. Translation: CAB14289.2.
PIRiB39440. UFBSD.
RefSeqiNP_390238.2. NC_000964.3.
WP_003230430.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP26899.
SMRiP26899.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP26899. 2 interactors.
STRINGi224308.Bsubs1_010100012936.

Proteomic databases

PaxDbiP26899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14289; CAB14289; BSU23570.
GeneIDi938721.
KEGGibsu:BSU23570.
PATRICi18976535. VBIBacSub10457_2458.

Phylogenomic databases

eggNOGiENOG4108IJ0. Bacteria.
COG1027. LUCA.
HOGENOMiHOG000061737.
InParanoidiP26899.
KOiK01744.
OMAiMMHSALK.
PhylomeDBiP26899.

Enzyme and pathway databases

BioCyciBSUB:BSU23570-MONOMER.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR004708. ApsA.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00839. aspA. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASPA_BACSU
AccessioniPrimary (citable) accession number: P26899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 7, 2009
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.