Reviewed, UniProtKB/Swiss-Prot P26899 (ASPA_BACSU)

Names and origin

Protein names

Recommended name:
    Aspartate ammonia-lyase
      Short name=Aspartase
    EC=4.3.1.1

Gene names

Name:	ansB
Ordered Locus Names:	BSU23570

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate = fumarate + NH3.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Aspartase subfamily.

Ontologies

Keywords
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	aspartate ammonia-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Aspartate ammonia-lyase		PRO_0000161336
Regions
	Region	143 – 145	3	Substrate binding By similarity
Sites
	Binding site	104	1	Substrate By similarity
Experimental info
	Sequence conflict	144	1	T → Q in AAA22244. Ref.1
	Sequence conflict	144	1	T → Q in BAA12643. Ref.2
	Sequence conflict	182	1	D → H in AAA22244. Ref.1
	Sequence conflict	182	1	D → H in BAA12643. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P26899-1 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: F3E847DC83A81460	FASTA	475	52,504
	10 20 30 40 50 60 MLNGQKEYRV EKDFLGEKQI EADVYYGIQT LRASENFPIT GYKIHEEMIN ALAIVKKAAA 70 80 90 100 110 120 LANMDVKRLY EGIGQAIVQA ADEILEGKWH DQFIVDPIQG GAGTSMNMNA NEVIGNRALE 130 140 150 160 170 180 IMGHKKGDYI HLSPNTHVNM SQSTNDVFPT AIHISTLKLL EKLLKTMEDM HSVFKQKAQE 190 200 210 220 230 240 FDSVIKMGRT HLQDAVPIRL GQEFEAYSRV LERDIKRIKQ SRQHLYEVNM GATAVGTGLN 250 260 270 280 290 300 ADPEYIKQVV KHLADISGLP LVGADHLVDA TQNTDAYTEV SASLKVCMMN MSKIANDLRL 310 320 330 340 350 360 MASGPRAGLA EISLPARQPG SSIMPGKVNP VMAELINQIA FQVIGNDNTI CLASEAGQLE 370 380 390 400 410 420 LNVMEPVLVF NLLQSISIMN NGFRSFTDNC LKGIEANEKR MKQYVEKSAG VITAVNPHLG 430 440 450 460 470 YEAAARIARE AIMTGQSVRD LCLQHDVLTE EELDIILNPY EMTKPGIAGK ELLEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase." Sun D., Setlow P. J. Bacteriol. 173:3831-3845(1991) [PubMed: 1711029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 144 AND 182.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63264 Genomic DNA. Translation: AAA22244.1. D84432 Genomic DNA. Translation: BAA12643.1. AL009126 Genomic DNA. Translation: CAB14289.2.
PIR	UFBSD. B39440.
RefSeq	NP_390238.2.
3D structure databases
HSSP	HSSP built from PDB template 1J3U based on UniProtKB Q9LCC6.
SMR	P26899. Positions 9-469.
ModBase	Search...
Genome annotation databases
GeneID	938721.
GenomeReviews	Gene locus BSU23570 in contig AL009126_GR.
KEGG	bsu:BSU23570.
NMPDR	fig|224308.1.peg.2362.
Organism-specific databases
SubtiList	BG10301. ansB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P26899.
OMA	TANPERL.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2356-MON.
BRENDA	4.3.1.1. 150.
Family and domain databases
InterPro	IPR004708. ApsA. IPR003031. D_crystallin. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00839. aspA. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPA_BACSU

Accession

Primary (citable) accession number: P26899

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 7, 2009
Last modified:	November 3, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)