P26875 (RNT1_TRIHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Guanyl-specific ribonuclease Th1 Short name=RNase Th1 EC=3.1.27.3
Organism	Trichoderma harzianum (Hypocrea lixii)
Taxonomic identifier	5544 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea › mitosporic Hypocrea › Trichoderma

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.
Sequence similarities	Belongs to the ribonuclease N1/T1 family.

Ontologies

Keywords
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro ribonuclease T1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 106	106	Guanyl-specific ribonuclease Th1		PRO_0000137375
Sites
	Active site	39	1	By similarity
	Active site	58	1	Proton acceptor By similarity
	Active site	92	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	5 ↔ 103		By similarity
	Disulfide bond	23 ↔ 84		Probable

Sequences

	Sequence		Length	Mass (Da)
	P26875 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 892A8BFE043AA022	FASTA	106	10,750
	10 20 30 40 50 60 DTATCGKVFY SASAVSAASN AACNYVRAGS TAGGSTYPHV YNNYEGFRFK GLSKPFYEFP 70 80 90 100 ILSSGKTYTG GSPGADRVVI NGQCSIAGII THTGASGNAF VACGGT

References

[1]

"Isolation, analysis of amino acid sequence and crystallization of the extracellular ribonuclease Th1 from Trichoderma harzianum-01." Bezborodova S.I., Vasileva-Tonkova E.S., Polyakov K.M., Shlyapnikov S.V. Bioorg. Khim. 14:453-466(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: 01.

Cross-references

Sequence databases
PIR	JN0428.
3D structure databases
ProteinModelPortal	P26875.
SMR	P26875. Positions 4-106.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.10.450.30. 1 hit.
InterPro	IPR000026. Gua-sp_ribonuclease_N1/T1. IPR016191. Ribonuclease/ribotoxin. [Graphical view]
Pfam	PF00545. Ribonuclease. 1 hit. [Graphical view]
SUPFAM	SSF53933. Ribonuclease/ribotoxin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNT1_TRIHA

Accession

Primary (citable) accession number: P26875

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program