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Protein

Chloramphenicol acetyltransferase

Gene

cat

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is an effector of chloramphenicol (Cm) resistance in bacteria. Acetylates Cm but not 1-acetoxy-Cm.

Catalytic activityi

Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei791

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BRENDAi2.3.1.28. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloramphenicol acetyltransferase (EC:2.3.1.28)
Alternative name(s):
Xenobiotic acetyltransferase
Short name:
XAT
Gene namesi
Name:cat
Synonyms:catB7
Ordered Locus Names:PA0706
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0706.

Pathology & Biotechi

Chemistry databases

DrugBankiDB00446. Chloramphenicol.
DB00878. Chlorhexidine.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000686611 – 212Chloramphenicol acetyltransferaseAdd BLAST212

Proteomic databases

PaxDbiP26841.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi208964.PA0706.

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 14Combined sources3
Turni15 – 17Combined sources3
Beta strandi23 – 25Combined sources3
Beta strandi30 – 32Combined sources3
Turni34 – 36Combined sources3
Helixi40 – 43Combined sources4
Beta strandi52 – 54Combined sources3
Beta strandi57 – 59Combined sources3
Beta strandi64 – 66Combined sources3
Beta strandi70 – 72Combined sources3
Turni75 – 78Combined sources4
Helixi89 – 91Combined sources3
Helixi96 – 100Combined sources5
Beta strandi111 – 113Combined sources3
Beta strandi151 – 154Combined sources4
Turni155 – 158Combined sources4
Beta strandi159 – 165Combined sources7
Helixi167 – 176Combined sources10
Helixi178 – 180Combined sources3
Helixi183 – 188Combined sources6
Helixi190 – 193Combined sources4
Helixi198 – 208Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XATX-ray3.20A1-212[»]
2XATX-ray3.20A1-212[»]
ProteinModelPortaliP26841.
SMRiP26841.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26841.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105EQK. Bacteria.
COG0110. LUCA.
InParanoidiP26841.
KOiK00638.
OMAiYSYYSGF.
PhylomeDBiP26841.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.

Sequencei

Sequence statusi: Complete.

P26841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNYFESPFR GKLLSEQVSN PNIRVGRYSY YSGYYHGHSF DDCARYLMPD
60 70 80 90 100
RDDVDKLVIG SFCSIGSGAA FIMAGNQGHR AEWASTFPFH FMHEEPVFAG
110 120 130 140 150
AVNGYQPAGD TLIGHDVWIG TEAMFMPGVR VGHGAIIGSR ALVTGDVEPY
160 170 180 190 200
AIVGGNPART IRKRFSDGDI QNLLEMAWWD WPLADIEAAM PLLCTGDIPA
210
LYRHWKQRQA TA
Length:212
Mass (Da):23,525
Last modified:January 11, 2001 - v3
Checksum:i306EEF39978C19FC
GO

Sequence cautioni

The sequence X12366 differs from that shown. Reason: Frameshift at positions 11, 18 and 38.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97V → A (PubMed:9188747).Curated1
Sequence conflicti116D → E (PubMed:9188747).Curated1
Sequence conflicti203R → Q (PubMed:9188747).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036933 Genomic DNA. Translation: AAD02068.1.
AE004091 Genomic DNA. Translation: AAG04095.1.
X12366 Genomic DNA. No translation available.
PIRiC83557.
RefSeqiNP_249397.1. NC_002516.2.
WP_003112709.1. NZ_ASJY01000115.1.

Genome annotation databases

EnsemblBacteriaiAAG04095; AAG04095; PA0706.
GeneIDi878374.
KEGGiag:AAD02068.
pae:PA0706.
PATRICi19835692. VBIPseAer58763_0740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036933 Genomic DNA. Translation: AAD02068.1.
AE004091 Genomic DNA. Translation: AAG04095.1.
X12366 Genomic DNA. No translation available.
PIRiC83557.
RefSeqiNP_249397.1. NC_002516.2.
WP_003112709.1. NZ_ASJY01000115.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XATX-ray3.20A1-212[»]
2XATX-ray3.20A1-212[»]
ProteinModelPortaliP26841.
SMRiP26841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0706.

Chemistry databases

DrugBankiDB00446. Chloramphenicol.
DB00878. Chlorhexidine.

Proteomic databases

PaxDbiP26841.

Protocols and materials databases

DNASUi878374.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04095; AAG04095; PA0706.
GeneIDi878374.
KEGGiag:AAD02068.
pae:PA0706.
PATRICi19835692. VBIPseAer58763_0740.

Organism-specific databases

PseudoCAPiPA0706.

Phylogenomic databases

eggNOGiENOG4105EQK. Bacteria.
COG0110. LUCA.
InParanoidiP26841.
KOiK00638.
OMAiYSYYSGF.
PhylomeDBiP26841.

Enzyme and pathway databases

BRENDAi2.3.1.28. 5087.

Miscellaneous databases

EvolutionaryTraceiP26841.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAT4_PSEAE
AccessioniPrimary (citable) accession number: P26841
Secondary accession number(s): Q9S542
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 11, 2001
Last modified: November 2, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.