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Protein

Chloramphenicol acetyltransferase

Gene

cat

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is an effector of chloramphenicol (Cm) resistance in bacteria. Acetylates Cm but not 1-acetoxy-Cm.

Catalytic activityi

Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BRENDAi2.3.1.28. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloramphenicol acetyltransferase (EC:2.3.1.28)
Alternative name(s):
Xenobiotic acetyltransferase
Short name:
XAT
Gene namesi
Name:cat
Synonyms:catB7
Ordered Locus Names:PA0706
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0706.

Pathology & Biotechi

Chemistry

DrugBankiDB00446. Chloramphenicol.
DB00878. Chlorhexidine.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Chloramphenicol acetyltransferasePRO_0000068661Add
BLAST

Proteomic databases

PaxDbiP26841.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi208964.PA0706.

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 143Combined sources
Turni15 – 173Combined sources
Beta strandi23 – 253Combined sources
Beta strandi30 – 323Combined sources
Turni34 – 363Combined sources
Helixi40 – 434Combined sources
Beta strandi52 – 543Combined sources
Beta strandi57 – 593Combined sources
Beta strandi64 – 663Combined sources
Beta strandi70 – 723Combined sources
Turni75 – 784Combined sources
Helixi89 – 913Combined sources
Helixi96 – 1005Combined sources
Beta strandi111 – 1133Combined sources
Beta strandi151 – 1544Combined sources
Turni155 – 1584Combined sources
Beta strandi159 – 1657Combined sources
Helixi167 – 17610Combined sources
Helixi178 – 1803Combined sources
Helixi183 – 1886Combined sources
Helixi190 – 1934Combined sources
Helixi198 – 20811Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XATX-ray3.20A1-212[»]
2XATX-ray3.20A1-212[»]
ProteinModelPortaliP26841.
SMRiP26841. Positions 3-210.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26841.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105EQK. Bacteria.
COG0110. LUCA.
InParanoidiP26841.
KOiK00638.
OMAiYSYYSGF.
PhylomeDBiP26841.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.

Sequencei

Sequence statusi: Complete.

P26841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNYFESPFR GKLLSEQVSN PNIRVGRYSY YSGYYHGHSF DDCARYLMPD
60 70 80 90 100
RDDVDKLVIG SFCSIGSGAA FIMAGNQGHR AEWASTFPFH FMHEEPVFAG
110 120 130 140 150
AVNGYQPAGD TLIGHDVWIG TEAMFMPGVR VGHGAIIGSR ALVTGDVEPY
160 170 180 190 200
AIVGGNPART IRKRFSDGDI QNLLEMAWWD WPLADIEAAM PLLCTGDIPA
210
LYRHWKQRQA TA
Length:212
Mass (Da):23,525
Last modified:January 11, 2001 - v3
Checksum:i306EEF39978C19FC
GO

Sequence cautioni

The sequence X12366 differs from that shown. Reason: Frameshift at positions 11, 18 and 38. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971V → A (PubMed:9188747).Curated
Sequence conflicti116 – 1161D → E (PubMed:9188747).Curated
Sequence conflicti203 – 2031R → Q (PubMed:9188747).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036933 Genomic DNA. Translation: AAD02068.1.
AE004091 Genomic DNA. Translation: AAG04095.1.
X12366 Genomic DNA. No translation available.
PIRiC83557.
RefSeqiNP_249397.1. NC_002516.2.
WP_003112709.1. NZ_ASJY01000115.1.

Genome annotation databases

EnsemblBacteriaiAAG04095; AAG04095; PA0706.
GeneIDi878374.
KEGGiag:AAD02068.
pae:PA0706.
PATRICi19835692. VBIPseAer58763_0740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036933 Genomic DNA. Translation: AAD02068.1.
AE004091 Genomic DNA. Translation: AAG04095.1.
X12366 Genomic DNA. No translation available.
PIRiC83557.
RefSeqiNP_249397.1. NC_002516.2.
WP_003112709.1. NZ_ASJY01000115.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XATX-ray3.20A1-212[»]
2XATX-ray3.20A1-212[»]
ProteinModelPortaliP26841.
SMRiP26841. Positions 3-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0706.

Chemistry

DrugBankiDB00446. Chloramphenicol.
DB00878. Chlorhexidine.

Proteomic databases

PaxDbiP26841.

Protocols and materials databases

DNASUi878374.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04095; AAG04095; PA0706.
GeneIDi878374.
KEGGiag:AAD02068.
pae:PA0706.
PATRICi19835692. VBIPseAer58763_0740.

Organism-specific databases

PseudoCAPiPA0706.

Phylogenomic databases

eggNOGiENOG4105EQK. Bacteria.
COG0110. LUCA.
InParanoidiP26841.
KOiK00638.
OMAiYSYYSGF.
PhylomeDBiP26841.

Enzyme and pathway databases

BRENDAi2.3.1.28. 5087.

Miscellaneous databases

EvolutionaryTraceiP26841.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAT4_PSEAE
AccessioniPrimary (citable) accession number: P26841
Secondary accession number(s): Q9S542
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 11, 2001
Last modified: September 7, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.