P26836 (LYS_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable autolytic lysozyme
EC=3.2.1.17

Alternative name(s):
1,4-beta-N-acetylmuramidase
Autolysin

Gene names

Name:	lyc
Ordered Locus Names:	CPE0382

Organism

Clostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195102 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium perfringens

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 25 family.

Ontologies

Keywords
Domain	Repeat
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Probable autolytic lysozyme		PRO_0000208265
Regions
	Repeat	230 – 252	23	1
	Repeat	253 – 272	20	2
	Repeat	273 – 295	23	3
	Repeat	296 – 318	23	4
	Repeat	319 – 342	24	5
	Region	230 – 342	113	5 X 23 AA tandem repeats
Sites
	Active site	13	1	By similarity
	Active site	103	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26836 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: EC3B4078A2FB4732	FASTA	342	39,558
	10 20 30 40 50 60 MEGRNSNNLK GIDVSNWQGN INFKSVKNEG IEVVYIKATE GDYFKDSYAK QNYKRAKAEG 70 80 90 100 110 120 LKVGFYHFFK PNKNAKRQAK YFIDYLNEIG ATDYDCKLAL DVETTEGRSA YELTTMCIEF 130 140 150 160 170 180 LEEVRKITNR EVVVYTYTSF ANNNLDNRLG VYPLWIAEYG VKAPKDNRVW SSWIGFQYSD 190 200 210 220 230 240 KGNVAGVSGN CDMNEFKEEI LDVKNNFKLY NATTKNISTY LNIREKGEID SKIIGKIPAG 250 260 270 280 290 300 EEFMIKWVDS NYLGWYLIEY KNITGYVSSK YVEKFQMATT YNVSDFLNVR ERGTTDSKVV 310 320 330 340 AIIDDGEIFR IDWVDSDYIG WYRITTKYGK NGFVKADFVK KI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.
[2]	"Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens." Canard B., Garnier T., Saint-Joanis B., Cole S.T. Mol. Gen. Genet. 243:215-224(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 256-342. Strain: CPN50.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB80088.1. M81878 Genomic DNA. Translation: AAA23261.1.
PIR	S43906.
RefSeq	NP_561298.1. NC_003366.1.
3D structure databases
ProteinModelPortal	P26836.
ModBase	Search...
Protein-protein interaction databases
STRING	195102.CPE0382.
Protein family/group databases
CAZy	GH25. Glycoside Hydrolase Family 25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB80088; BAB80088; BAB80088.
GeneID	988635.
KEGG	cpe:CPE0382.
PATRIC	19494672. VBICloPer59675_0441.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3757.
HOGENOM	HOG000113426.
KO	K07273.
OMA	TEGRSAY.
ProtClustDB	CLSK566944.
Enzyme and pathway databases
BioCyc	CPER195102:GJFM-419-MONOMER.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR002053. Glyco_hydro_25. IPR008270. Glyco_hydro_25_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR018077. Glyco_hydro_fam25_subgr. IPR017853. Glycoside_hydrolase_SF. IPR003646. SH3-like_bac. [Graphical view]
Pfam	PF01183. Glyco_hydro_25. 1 hit. PF08239. SH3_3. 2 hits. [Graphical view]
SMART	SM00641. Glyco_25. 1 hit. SM00287. SH3b. 2 hits. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00953. GLYCOSYL_HYDROL_F25. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS_CLOPE

Accession

Primary (citable) accession number: P26836

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 31, 2002
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program