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P26831

- NAGH_CLOPE

UniProt

P26831 - NAGH_CLOPE

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Protein

Hyaluronoglucosaminidase

Gene

nagH

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Putative virulence factor which is likely to act on connective tissue during gas gangrene.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. pathogenesis Source: UniProtKB-KW
  3. polysaccharide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Toxin

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciCPER195102:GJFM-216-MONOMER.
BRENDAi4.2.2.1. 1503.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH84. Glycoside Hydrolase Family 84.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronoglucosaminidase (EC:3.2.1.35)
Short name:
Hyaluronidase
Alternative name(s):
Mu toxin
Gene namesi
Name:nagH
Ordered Locus Names:CPE0191
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 16281598HyaluronoglucosaminidasePRO_0000021787Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-46268N.
STRINGi195102.CPE0191.

Structurei

Secondary structure

1
1628
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi809 – 8124Combined sources
Beta strandi817 – 8215Combined sources
Helixi823 – 8264Combined sources
Beta strandi827 – 8293Combined sources
Beta strandi835 – 8384Combined sources
Helixi840 – 8434Combined sources
Beta strandi850 – 86819Combined sources
Turni873 – 8764Combined sources
Beta strandi880 – 89213Combined sources
Beta strandi894 – 9007Combined sources
Beta strandi909 – 92820Combined sources
Beta strandi932 – 9343Combined sources
Beta strandi936 – 9449Combined sources
Helixi1500 – 151516Combined sources
Beta strandi1518 – 15214Combined sources
Helixi1529 – 154416Combined sources
Helixi1551 – 157020Combined sources
Turni1575 – 15784Combined sources
Beta strandi1583 – 15853Combined sources
Helixi1588 – 15969Combined sources
Turni1597 – 15993Combined sources
Turni1604 – 16063Combined sources
Beta strandi1611 – 16133Combined sources
Helixi1616 – 162712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JNKNMR-A1498-1628[»]
2OZNX-ray1.60B1498-1628[»]
2W1QX-ray1.60A/B807-975[»]
2W1SX-ray1.45A/B807-975[»]
2W1UX-ray2.00A/B/C/D807-975[»]
2WDBX-ray2.03A/B/C/D807-975[»]
ProteinModelPortaliP26831.
SMRiP26831. Positions 1498-1628.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26831.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini781 – 953173F5/8 type CPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 F5/8 type C domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG69445.
HOGENOMiHOG000113726.
KOiK01197.
OMAiHTEYAEV.
OrthoDBiEOG6QVRC1.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.260. 3 hits.
3.30.379.10. 1 hit.
InterProiIPR011496. Beta-N-acetylglucosaminidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR011490. FIVAR_dom.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 2 hits.
PF07554. FIVAR. 3 hits.
PF02838. Glyco_hydro_20b. 1 hit.
PF07555. NAGidase. 1 hit.
[Graphical view]
SMARTiSM00231. FA58C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 5 hits.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS50022. FA58C_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26831-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKNIRKIIT STVLAAMTIS VLPSNLVVFA TDGITENFYE IYPKPQEISY
60 70 80 90 100
SGGEFQISDE INIVYDDGID TYTKKRVDEV LEASNLEATV SNEIVPGKTN
110 120 130 140 150
FLVGINESGG VVDNYFNKNI PHDESFFDEK MDANIVSVKD GVIGVIGEDT
160 170 180 190 200
DSAFYGVTTL KHVFNQLEEG NKIQSFRADD YAEVAHRGFI EGYYGNPWSN
210 220 230 240 250
EDRAELMKFG GDYKLNQYVF APKDDPYHNS KWRDLYPEEK LSEIKKLAQV
260 270 280 290 300
GNETKNRYVY ALHPFMNNPV RFDTEENYQN DLGVIKAKFT QLLENDVRQF
310 320 330 340 350
AILADDASAP AQGASMYVKL LTDLTRWLEE QQSTYPDLKT DLMFCPSDYY
360 370 380 390 400
GNGSSAQLKE LNKAEDNVSI VMTGGRIWGE VDENFANNFM NNISTEGHPG
410 420 430 440 450
RAPFFWINWP CSDNSKQHLI MGGNDTFLHP GVDPSKIDGI VLNPMQQAEA
460 470 480 490 500
NKSALFAIAD YAWNIWDNKE EADENWNDSF KYMDHGTAEE TNSSLALREI
510 520 530 540 550
SKHMINQNMD GRVRPLQESV ELAPKLEAFK QKYDSGASIK EDALELIAEF
560 570 580 590 600
TNLQKAADYY KNNPGNERTR DQIIYWLNCW EDTMDAAIGY LKSAIAIEEG
610 620 630 640 650
DDEAAWANYS EAQGAFEKSK TYGFHYVDHT EYAEVGVQHI VPFIKSMGQN
660 670 680 690 700
LSVVIGSIVD PNRIIATYIS NRQDAPTGNP DNIFDNNAST ELVYKNPNRI
710 720 730 740 750
DVGTYVGVKY SNPITLNNVE FLMGANSNPN DTMQKAKIQY TVDGREWIDL
760 770 780 790 800
EEGVEYTMPG AIKVENLDLK VRGVRLIATE ARENTWLGVR DINVNKKEDS
810 820 830 840 850
NSGVEFNPSL IRSESWQVYE GNEANLLDGD DNTGVWYKTL NGDTSLAGEF
860 870 880 890 900
IGLDLGKEIK LDGIRFVIGK NGGGSSDKWN KFKLEYSLDN ESWTTIKEYD
910 920 930 940 950
KTGAPAGKDV IEESFETPIS AKYIRLTNME NINKWLTFSE FAIISDELEN
960 970 980 990 1000
AGNKENVYTN TELDLLSLAK EDVTKLIPTD DISLNHGEYI GVKLNRIKDL
1010 1020 1030 1040 1050
SNINLEISND TGLKLQSSMN GVEWTEITDK NTLEDGRYVR LINTSNEAVN
1060 1070 1080 1090 1100
FNLTKFEVNS NEVYEPSLVD AYVGDDGAKK AVDGDLKTRV KFLGAPSTGD
1110 1120 1130 1140 1150
TIVYDLGQEI LVDNLKYVVL DTEVDHVRDG KIQLSLDGET WTDAITIGDG
1160 1170 1180 1190 1200
VENGVDDMFS TPLKNGYKHG NQSGGIVPID SAYVEGDNLN QKARYVRILF
1210 1220 1230 1240 1250
TAPYRHRWTV INELMINNGE YISTVNDPTY ISNPIEERGF APSNLRDGNL
1260 1270 1280 1290 1300
TTSYKPNTNN GEISEGSITY RLSEKTDVRK VTIVQSGSSI SNAKVMARVG
1310 1320 1330 1340 1350
DGSENVTDQW VQLGTLSNSL NEFINRDYNN IYEIKIEWTD VAPNIYEIIT
1360 1370 1380 1390 1400
LNQEFEFPVN DSLKAKYDEL INLSGDEYTL SSFETLKEAL NEAKSILDDS
1410 1420 1430 1440 1450
NSSQKKIDKA LEKLNKAEER LDLRATDFED FNKVLTLGNS LVEEEYTAES
1460 1470 1480 1490 1500
WALFSEVLEA ANEANKNKAD YTQDQINQIV IDLDASIKAL VKETPEVDKT
1510 1520 1530 1540 1550
NLGELINQGK SLLDESVEGF NVGEYHKGAK DGLTVEINKA EEVFNKEDAT
1560 1570 1580 1590 1600
EEEINLAKES LEGAIARFNS LLIEESTGDF NGNGKIDIGD LAMVSKNIGS
1610 1620
TTNTSLDLNK DGSIDEYEIS FINHRILN
Length:1,628
Mass (Da):182,475
Last modified:January 31, 2002 - v2
Checksum:iD4252A2512BBED69
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti147 – 1471G → A in strain: CPN50.
Natural varianti172 – 1754KIQS → EIKN in strain: CPN50.
Natural varianti250 – 2501V → M in strain: CPN50.
Natural varianti548 – 5481A → E in strain: CPN50.
Natural varianti558 – 5581D → E in strain: CPN50.
Natural varianti614 – 6141G → S in strain: CPN50.
Natural varianti944 – 9441I → V in strain: CPN50.
Natural varianti950 – 9501N → S in strain: CPN50.
Natural varianti979 – 9791T → I in strain: CPN50.
Natural varianti982 – 9821I → L in strain: CPN50.
Natural varianti1042 – 10421I → F in strain: CPN50.
Natural varianti1043 – 1628586Missing in strain: CPN50.
Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81878 Genomic DNA. Translation: AAA23259.1.
BA000016 Genomic DNA. Translation: BAB79897.1.
PIRiS43904.
RefSeqiNP_561107.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB79897; BAB79897; BAB79897.
GeneIDi988432.
KEGGicpe:CPE0191.
PATRICi19494262. VBICloPer59675_0248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81878 Genomic DNA. Translation: AAA23259.1 .
BA000016 Genomic DNA. Translation: BAB79897.1 .
PIRi S43904.
RefSeqi NP_561107.1. NC_003366.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JNK NMR - A 1498-1628 [» ]
2OZN X-ray 1.60 B 1498-1628 [» ]
2W1Q X-ray 1.60 A/B 807-975 [» ]
2W1S X-ray 1.45 A/B 807-975 [» ]
2W1U X-ray 2.00 A/B/C/D 807-975 [» ]
2WDB X-ray 2.03 A/B/C/D 807-975 [» ]
ProteinModelPortali P26831.
SMRi P26831. Positions 1498-1628.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-46268N.
STRINGi 195102.CPE0191.

Protein family/group databases

CAZyi CBM32. Carbohydrate-Binding Module Family 32.
GH84. Glycoside Hydrolase Family 84.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB79897 ; BAB79897 ; BAB79897 .
GeneIDi 988432.
KEGGi cpe:CPE0191.
PATRICi 19494262. VBICloPer59675_0248.

Phylogenomic databases

eggNOGi NOG69445.
HOGENOMi HOG000113726.
KOi K01197.
OMAi HTEYAEV.
OrthoDBi EOG6QVRC1.

Enzyme and pathway databases

BioCyci CPER195102:GJFM-216-MONOMER.
BRENDAi 4.2.2.1. 1503.

Miscellaneous databases

EvolutionaryTracei P26831.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
2.60.120.260. 3 hits.
3.30.379.10. 1 hit.
InterProi IPR011496. Beta-N-acetylglucosaminidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR011490. FIVAR_dom.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
[Graphical view ]
Pfami PF00754. F5_F8_type_C. 2 hits.
PF07554. FIVAR. 3 hits.
PF02838. Glyco_hydro_20b. 1 hit.
PF07555. NAGidase. 1 hit.
[Graphical view ]
SMARTi SM00231. FA58C. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 5 hits.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEi PS50022. FA58C_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens."
    Canard B., Garnier T., Saint-Joanis B., Cole S.T.
    Mol. Gen. Genet. 243:215-224(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CPN50.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiNAGH_CLOPE
AccessioniPrimary (citable) accession number: P26831
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 31, 2002
Last modified: November 26, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The partially purified protein from strain CPN50 is approximately 70 kDa smaller than the sequence indicated here.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3