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Protein

Hyaluronoglucosaminidase

Gene

nagH

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative virulence factor which is likely to act on connective tissue during gas gangrene.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Toxin

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BRENDAi4.2.2.1. 1503.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH84. Glycoside Hydrolase Family 84.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronoglucosaminidase (EC:3.2.1.35)
Short name:
Hyaluronidase
Alternative name(s):
Mu toxin
Gene namesi
Name:nagH
Ordered Locus Names:CPE0191
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000002178731 – 1628HyaluronoglucosaminidaseAdd BLAST1598

Interactioni

Protein-protein interaction databases

DIPiDIP-46268N.

Structurei

Secondary structure

11628
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi809 – 812Combined sources4
Beta strandi817 – 821Combined sources5
Helixi823 – 826Combined sources4
Beta strandi827 – 829Combined sources3
Beta strandi835 – 838Combined sources4
Helixi840 – 843Combined sources4
Beta strandi850 – 868Combined sources19
Turni873 – 876Combined sources4
Beta strandi880 – 892Combined sources13
Beta strandi894 – 900Combined sources7
Beta strandi909 – 928Combined sources20
Beta strandi932 – 934Combined sources3
Beta strandi936 – 944Combined sources9
Turni946 – 948Combined sources3
Helixi1500 – 1515Combined sources16
Beta strandi1518 – 1521Combined sources4
Helixi1529 – 1544Combined sources16
Helixi1551 – 1570Combined sources20
Turni1575 – 1578Combined sources4
Beta strandi1583 – 1585Combined sources3
Helixi1588 – 1596Combined sources9
Turni1597 – 1599Combined sources3
Turni1604 – 1606Combined sources3
Beta strandi1611 – 1613Combined sources3
Helixi1616 – 1627Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JNKNMR-A1498-1628[»]
2OZNX-ray1.60B1498-1628[»]
2W1QX-ray1.60A/B807-975[»]
2W1SX-ray1.45A/B807-975[»]
2W1UX-ray2.00A/B/C/D807-975[»]
2WDBX-ray2.03A/B/C/D807-975[»]
ProteinModelPortaliP26831.
SMRiP26831.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26831.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini781 – 953F5/8 type CPROSITE-ProRule annotationAdd BLAST173
Domaini1573 – 1628DockerinPROSITE-ProRule annotationAdd BLAST56

Sequence similaritiesi

Contains 1 dockerin domain.PROSITE-ProRule annotation
Contains 1 F5/8 type C domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000113726.
KOiK01197.
OMAiYAEVGVQ.
OrthoDBiPOG091H0IJT.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.260. 3 hits.
3.30.379.10. 1 hit.
InterProiIPR011496. Beta-N-acetylglucosaminidase.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR016134. Dockerin_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR000421. FA58C.
IPR011490. FIVAR_dom.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 3 hits.
PF07554. FIVAR. 3 hits.
PF02838. Glyco_hydro_20b. 1 hit.
PF07555. NAGidase. 1 hit.
[Graphical view]
SMARTiSM00231. FA58C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 5 hits.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS51766. DOCKERIN. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26831-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKNIRKIIT STVLAAMTIS VLPSNLVVFA TDGITENFYE IYPKPQEISY
60 70 80 90 100
SGGEFQISDE INIVYDDGID TYTKKRVDEV LEASNLEATV SNEIVPGKTN
110 120 130 140 150
FLVGINESGG VVDNYFNKNI PHDESFFDEK MDANIVSVKD GVIGVIGEDT
160 170 180 190 200
DSAFYGVTTL KHVFNQLEEG NKIQSFRADD YAEVAHRGFI EGYYGNPWSN
210 220 230 240 250
EDRAELMKFG GDYKLNQYVF APKDDPYHNS KWRDLYPEEK LSEIKKLAQV
260 270 280 290 300
GNETKNRYVY ALHPFMNNPV RFDTEENYQN DLGVIKAKFT QLLENDVRQF
310 320 330 340 350
AILADDASAP AQGASMYVKL LTDLTRWLEE QQSTYPDLKT DLMFCPSDYY
360 370 380 390 400
GNGSSAQLKE LNKAEDNVSI VMTGGRIWGE VDENFANNFM NNISTEGHPG
410 420 430 440 450
RAPFFWINWP CSDNSKQHLI MGGNDTFLHP GVDPSKIDGI VLNPMQQAEA
460 470 480 490 500
NKSALFAIAD YAWNIWDNKE EADENWNDSF KYMDHGTAEE TNSSLALREI
510 520 530 540 550
SKHMINQNMD GRVRPLQESV ELAPKLEAFK QKYDSGASIK EDALELIAEF
560 570 580 590 600
TNLQKAADYY KNNPGNERTR DQIIYWLNCW EDTMDAAIGY LKSAIAIEEG
610 620 630 640 650
DDEAAWANYS EAQGAFEKSK TYGFHYVDHT EYAEVGVQHI VPFIKSMGQN
660 670 680 690 700
LSVVIGSIVD PNRIIATYIS NRQDAPTGNP DNIFDNNAST ELVYKNPNRI
710 720 730 740 750
DVGTYVGVKY SNPITLNNVE FLMGANSNPN DTMQKAKIQY TVDGREWIDL
760 770 780 790 800
EEGVEYTMPG AIKVENLDLK VRGVRLIATE ARENTWLGVR DINVNKKEDS
810 820 830 840 850
NSGVEFNPSL IRSESWQVYE GNEANLLDGD DNTGVWYKTL NGDTSLAGEF
860 870 880 890 900
IGLDLGKEIK LDGIRFVIGK NGGGSSDKWN KFKLEYSLDN ESWTTIKEYD
910 920 930 940 950
KTGAPAGKDV IEESFETPIS AKYIRLTNME NINKWLTFSE FAIISDELEN
960 970 980 990 1000
AGNKENVYTN TELDLLSLAK EDVTKLIPTD DISLNHGEYI GVKLNRIKDL
1010 1020 1030 1040 1050
SNINLEISND TGLKLQSSMN GVEWTEITDK NTLEDGRYVR LINTSNEAVN
1060 1070 1080 1090 1100
FNLTKFEVNS NEVYEPSLVD AYVGDDGAKK AVDGDLKTRV KFLGAPSTGD
1110 1120 1130 1140 1150
TIVYDLGQEI LVDNLKYVVL DTEVDHVRDG KIQLSLDGET WTDAITIGDG
1160 1170 1180 1190 1200
VENGVDDMFS TPLKNGYKHG NQSGGIVPID SAYVEGDNLN QKARYVRILF
1210 1220 1230 1240 1250
TAPYRHRWTV INELMINNGE YISTVNDPTY ISNPIEERGF APSNLRDGNL
1260 1270 1280 1290 1300
TTSYKPNTNN GEISEGSITY RLSEKTDVRK VTIVQSGSSI SNAKVMARVG
1310 1320 1330 1340 1350
DGSENVTDQW VQLGTLSNSL NEFINRDYNN IYEIKIEWTD VAPNIYEIIT
1360 1370 1380 1390 1400
LNQEFEFPVN DSLKAKYDEL INLSGDEYTL SSFETLKEAL NEAKSILDDS
1410 1420 1430 1440 1450
NSSQKKIDKA LEKLNKAEER LDLRATDFED FNKVLTLGNS LVEEEYTAES
1460 1470 1480 1490 1500
WALFSEVLEA ANEANKNKAD YTQDQINQIV IDLDASIKAL VKETPEVDKT
1510 1520 1530 1540 1550
NLGELINQGK SLLDESVEGF NVGEYHKGAK DGLTVEINKA EEVFNKEDAT
1560 1570 1580 1590 1600
EEEINLAKES LEGAIARFNS LLIEESTGDF NGNGKIDIGD LAMVSKNIGS
1610 1620
TTNTSLDLNK DGSIDEYEIS FINHRILN
Length:1,628
Mass (Da):182,475
Last modified:January 31, 2002 - v2
Checksum:iD4252A2512BBED69
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti147G → A in strain: CPN50. 1
Natural varianti172 – 175KIQS → EIKN in strain: CPN50. 4
Natural varianti250V → M in strain: CPN50. 1
Natural varianti548A → E in strain: CPN50. 1
Natural varianti558D → E in strain: CPN50. 1
Natural varianti614G → S in strain: CPN50. 1
Natural varianti944I → V in strain: CPN50. 1
Natural varianti950N → S in strain: CPN50. 1
Natural varianti979T → I in strain: CPN50. 1
Natural varianti982I → L in strain: CPN50. 1
Natural varianti1042I → F in strain: CPN50. 1
Natural varianti1043 – 1628Missing in strain: CPN50. Add BLAST586

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81878 Genomic DNA. Translation: AAA23259.1.
BA000016 Genomic DNA. Translation: BAB79897.1.
PIRiS43904.
RefSeqiWP_011009663.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB79897; BAB79897; BAB79897.
KEGGicpe:CPE0191.
PATRICi19494262. VBICloPer59675_0248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81878 Genomic DNA. Translation: AAA23259.1.
BA000016 Genomic DNA. Translation: BAB79897.1.
PIRiS43904.
RefSeqiWP_011009663.1. NC_003366.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JNKNMR-A1498-1628[»]
2OZNX-ray1.60B1498-1628[»]
2W1QX-ray1.60A/B807-975[»]
2W1SX-ray1.45A/B807-975[»]
2W1UX-ray2.00A/B/C/D807-975[»]
2WDBX-ray2.03A/B/C/D807-975[»]
ProteinModelPortaliP26831.
SMRiP26831.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46268N.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH84. Glycoside Hydrolase Family 84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB79897; BAB79897; BAB79897.
KEGGicpe:CPE0191.
PATRICi19494262. VBICloPer59675_0248.

Phylogenomic databases

HOGENOMiHOG000113726.
KOiK01197.
OMAiYAEVGVQ.
OrthoDBiPOG091H0IJT.

Enzyme and pathway databases

BRENDAi4.2.2.1. 1503.

Miscellaneous databases

EvolutionaryTraceiP26831.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.260. 3 hits.
3.30.379.10. 1 hit.
InterProiIPR011496. Beta-N-acetylglucosaminidase.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR016134. Dockerin_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR000421. FA58C.
IPR011490. FIVAR_dom.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 3 hits.
PF07554. FIVAR. 3 hits.
PF02838. Glyco_hydro_20b. 1 hit.
PF07555. NAGidase. 1 hit.
[Graphical view]
SMARTiSM00231. FA58C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 5 hits.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS51766. DOCKERIN. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAGH_CLOPE
AccessioniPrimary (citable) accession number: P26831
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 31, 2002
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The partially purified protein from strain CPN50 is approximately 70 kDa smaller than the sequence indicated here.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.