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P26831

- NAGH_CLOPE

UniProt

P26831 - NAGH_CLOPE

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Protein
Hyaluronoglucosaminidase
Gene
nagH, CPE0191
Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Putative virulence factor which is likely to act on connective tissue during gas gangrene.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. pathogenesis Source: UniProtKB-KW
  3. polysaccharide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Toxin

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciCPER195102:GJFM-216-MONOMER.
BRENDAi4.2.2.1. 1503.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH84. Glycoside Hydrolase Family 84.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronoglucosaminidase (EC:3.2.1.35)
Short name:
Hyaluronidase
Alternative name(s):
Mu toxin
Gene namesi
Name:nagH
Ordered Locus Names:CPE0191
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030 Reviewed prediction
Add
BLAST
Chaini31 – 16281598Hyaluronoglucosaminidase
PRO_0000021787Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-46268N.
STRINGi195102.CPE0191.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi809 – 8124
Beta strandi817 – 8215
Helixi823 – 8264
Beta strandi827 – 8293
Beta strandi835 – 8384
Helixi840 – 8434
Beta strandi850 – 86819
Turni873 – 8764
Beta strandi880 – 89213
Beta strandi894 – 9007
Beta strandi909 – 92820
Beta strandi932 – 9343
Beta strandi936 – 9449
Helixi1500 – 151516
Beta strandi1518 – 15214
Helixi1529 – 154416
Helixi1551 – 157020
Turni1575 – 15784
Beta strandi1583 – 15853
Helixi1588 – 15969
Turni1597 – 15993
Turni1604 – 16063
Beta strandi1611 – 16133
Helixi1616 – 162712

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JNKNMR-A1498-1628[»]
2OZNX-ray1.60B1498-1628[»]
2W1QX-ray1.60A/B807-975[»]
2W1SX-ray1.45A/B807-975[»]
2W1UX-ray2.00A/B/C/D807-975[»]
2WDBX-ray2.03A/B/C/D807-975[»]
ProteinModelPortaliP26831.
SMRiP26831. Positions 1498-1628.

Miscellaneous databases

EvolutionaryTraceiP26831.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini781 – 953173F5/8 type C
Add
BLAST

Sequence similaritiesi

Contains 1 F5/8 type C domain.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG69445.
HOGENOMiHOG000113726.
KOiK01197.
OMAiHTEYAEV.
OrthoDBiEOG6QVRC1.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.260. 3 hits.
3.30.379.10. 1 hit.
InterProiIPR011496. Beta-N-acetylglucosaminidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR011490. FIVAR_dom.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 2 hits.
PF07554. FIVAR. 3 hits.
PF02838. Glyco_hydro_20b. 1 hit.
PF07555. NAGidase. 1 hit.
[Graphical view]
SMARTiSM00231. FA58C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 5 hits.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS50022. FA58C_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26831-1 [UniParc]FASTAAdd to Basket

« Hide

MNKNIRKIIT STVLAAMTIS VLPSNLVVFA TDGITENFYE IYPKPQEISY     50
SGGEFQISDE INIVYDDGID TYTKKRVDEV LEASNLEATV SNEIVPGKTN 100
FLVGINESGG VVDNYFNKNI PHDESFFDEK MDANIVSVKD GVIGVIGEDT 150
DSAFYGVTTL KHVFNQLEEG NKIQSFRADD YAEVAHRGFI EGYYGNPWSN 200
EDRAELMKFG GDYKLNQYVF APKDDPYHNS KWRDLYPEEK LSEIKKLAQV 250
GNETKNRYVY ALHPFMNNPV RFDTEENYQN DLGVIKAKFT QLLENDVRQF 300
AILADDASAP AQGASMYVKL LTDLTRWLEE QQSTYPDLKT DLMFCPSDYY 350
GNGSSAQLKE LNKAEDNVSI VMTGGRIWGE VDENFANNFM NNISTEGHPG 400
RAPFFWINWP CSDNSKQHLI MGGNDTFLHP GVDPSKIDGI VLNPMQQAEA 450
NKSALFAIAD YAWNIWDNKE EADENWNDSF KYMDHGTAEE TNSSLALREI 500
SKHMINQNMD GRVRPLQESV ELAPKLEAFK QKYDSGASIK EDALELIAEF 550
TNLQKAADYY KNNPGNERTR DQIIYWLNCW EDTMDAAIGY LKSAIAIEEG 600
DDEAAWANYS EAQGAFEKSK TYGFHYVDHT EYAEVGVQHI VPFIKSMGQN 650
LSVVIGSIVD PNRIIATYIS NRQDAPTGNP DNIFDNNAST ELVYKNPNRI 700
DVGTYVGVKY SNPITLNNVE FLMGANSNPN DTMQKAKIQY TVDGREWIDL 750
EEGVEYTMPG AIKVENLDLK VRGVRLIATE ARENTWLGVR DINVNKKEDS 800
NSGVEFNPSL IRSESWQVYE GNEANLLDGD DNTGVWYKTL NGDTSLAGEF 850
IGLDLGKEIK LDGIRFVIGK NGGGSSDKWN KFKLEYSLDN ESWTTIKEYD 900
KTGAPAGKDV IEESFETPIS AKYIRLTNME NINKWLTFSE FAIISDELEN 950
AGNKENVYTN TELDLLSLAK EDVTKLIPTD DISLNHGEYI GVKLNRIKDL 1000
SNINLEISND TGLKLQSSMN GVEWTEITDK NTLEDGRYVR LINTSNEAVN 1050
FNLTKFEVNS NEVYEPSLVD AYVGDDGAKK AVDGDLKTRV KFLGAPSTGD 1100
TIVYDLGQEI LVDNLKYVVL DTEVDHVRDG KIQLSLDGET WTDAITIGDG 1150
VENGVDDMFS TPLKNGYKHG NQSGGIVPID SAYVEGDNLN QKARYVRILF 1200
TAPYRHRWTV INELMINNGE YISTVNDPTY ISNPIEERGF APSNLRDGNL 1250
TTSYKPNTNN GEISEGSITY RLSEKTDVRK VTIVQSGSSI SNAKVMARVG 1300
DGSENVTDQW VQLGTLSNSL NEFINRDYNN IYEIKIEWTD VAPNIYEIIT 1350
LNQEFEFPVN DSLKAKYDEL INLSGDEYTL SSFETLKEAL NEAKSILDDS 1400
NSSQKKIDKA LEKLNKAEER LDLRATDFED FNKVLTLGNS LVEEEYTAES 1450
WALFSEVLEA ANEANKNKAD YTQDQINQIV IDLDASIKAL VKETPEVDKT 1500
NLGELINQGK SLLDESVEGF NVGEYHKGAK DGLTVEINKA EEVFNKEDAT 1550
EEEINLAKES LEGAIARFNS LLIEESTGDF NGNGKIDIGD LAMVSKNIGS 1600
TTNTSLDLNK DGSIDEYEIS FINHRILN 1628
Length:1,628
Mass (Da):182,475
Last modified:January 31, 2002 - v2
Checksum:iD4252A2512BBED69
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti147 – 1471G → A in strain: CPN50.
Natural varianti172 – 1754KIQS → EIKN in strain: CPN50.
Natural varianti250 – 2501V → M in strain: CPN50.
Natural varianti548 – 5481A → E in strain: CPN50.
Natural varianti558 – 5581D → E in strain: CPN50.
Natural varianti614 – 6141G → S in strain: CPN50.
Natural varianti944 – 9441I → V in strain: CPN50.
Natural varianti950 – 9501N → S in strain: CPN50.
Natural varianti979 – 9791T → I in strain: CPN50.
Natural varianti982 – 9821I → L in strain: CPN50.
Natural varianti1042 – 10421I → F in strain: CPN50.
Natural varianti1043 – 1628586Missing in strain: CPN50.
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M81878 Genomic DNA. Translation: AAA23259.1.
BA000016 Genomic DNA. Translation: BAB79897.1.
PIRiS43904.
RefSeqiNP_561107.1. NC_003366.1.
WP_011009663.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB79897; BAB79897; BAB79897.
GeneIDi988432.
KEGGicpe:CPE0191.
PATRICi19494262. VBICloPer59675_0248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M81878 Genomic DNA. Translation: AAA23259.1 .
BA000016 Genomic DNA. Translation: BAB79897.1 .
PIRi S43904.
RefSeqi NP_561107.1. NC_003366.1.
WP_011009663.1. NC_003366.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JNK NMR - A 1498-1628 [» ]
2OZN X-ray 1.60 B 1498-1628 [» ]
2W1Q X-ray 1.60 A/B 807-975 [» ]
2W1S X-ray 1.45 A/B 807-975 [» ]
2W1U X-ray 2.00 A/B/C/D 807-975 [» ]
2WDB X-ray 2.03 A/B/C/D 807-975 [» ]
ProteinModelPortali P26831.
SMRi P26831. Positions 1498-1628.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-46268N.
STRINGi 195102.CPE0191.

Protein family/group databases

CAZyi CBM32. Carbohydrate-Binding Module Family 32.
GH84. Glycoside Hydrolase Family 84.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB79897 ; BAB79897 ; BAB79897 .
GeneIDi 988432.
KEGGi cpe:CPE0191.
PATRICi 19494262. VBICloPer59675_0248.

Phylogenomic databases

eggNOGi NOG69445.
HOGENOMi HOG000113726.
KOi K01197.
OMAi HTEYAEV.
OrthoDBi EOG6QVRC1.

Enzyme and pathway databases

BioCyci CPER195102:GJFM-216-MONOMER.
BRENDAi 4.2.2.1. 1503.

Miscellaneous databases

EvolutionaryTracei P26831.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
2.60.120.260. 3 hits.
3.30.379.10. 1 hit.
InterProi IPR011496. Beta-N-acetylglucosaminidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR011490. FIVAR_dom.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
[Graphical view ]
Pfami PF00754. F5_F8_type_C. 2 hits.
PF07554. FIVAR. 3 hits.
PF02838. Glyco_hydro_20b. 1 hit.
PF07555. NAGidase. 1 hit.
[Graphical view ]
SMARTi SM00231. FA58C. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 5 hits.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEi PS50022. FA58C_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens."
    Canard B., Garnier T., Saint-Joanis B., Cole S.T.
    Mol. Gen. Genet. 243:215-224(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CPN50.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiNAGH_CLOPE
AccessioniPrimary (citable) accession number: P26831
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 31, 2002
Last modified: September 3, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The partially purified protein from strain CPN50 is approximately 70 kDa smaller than the sequence indicated here.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi