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P26831

- NAGH_CLOPE

UniProt

P26831 - NAGH_CLOPE

Protein

Hyaluronoglucosaminidase

Gene

nagH

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (31 Jan 2002)
      Previous versions | rss
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    Functioni

    Putative virulence factor which is likely to act on connective tissue during gas gangrene.

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

    GO - Molecular functioni

    1. hyalurononglucosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell adhesion Source: InterPro
    2. pathogenesis Source: UniProtKB-KW
    3. polysaccharide catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Toxin

    Keywords - Biological processi

    Virulence

    Enzyme and pathway databases

    BioCyciCPER195102:GJFM-216-MONOMER.
    BRENDAi4.2.2.1. 1503.

    Protein family/group databases

    CAZyiCBM32. Carbohydrate-Binding Module Family 32.
    GH84. Glycoside Hydrolase Family 84.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronoglucosaminidase (EC:3.2.1.35)
    Short name:
    Hyaluronidase
    Alternative name(s):
    Mu toxin
    Gene namesi
    Name:nagH
    Ordered Locus Names:CPE0191
    OrganismiClostridium perfringens (strain 13 / Type A)
    Taxonomic identifieri195102 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000818: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 16281598HyaluronoglucosaminidasePRO_0000021787Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-46268N.
    STRINGi195102.CPE0191.

    Structurei

    Secondary structure

    1
    1628
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi809 – 8124
    Beta strandi817 – 8215
    Helixi823 – 8264
    Beta strandi827 – 8293
    Beta strandi835 – 8384
    Helixi840 – 8434
    Beta strandi850 – 86819
    Turni873 – 8764
    Beta strandi880 – 89213
    Beta strandi894 – 9007
    Beta strandi909 – 92820
    Beta strandi932 – 9343
    Beta strandi936 – 9449
    Helixi1500 – 151516
    Beta strandi1518 – 15214
    Helixi1529 – 154416
    Helixi1551 – 157020
    Turni1575 – 15784
    Beta strandi1583 – 15853
    Helixi1588 – 15969
    Turni1597 – 15993
    Turni1604 – 16063
    Beta strandi1611 – 16133
    Helixi1616 – 162712

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JNKNMR-A1498-1628[»]
    2OZNX-ray1.60B1498-1628[»]
    2W1QX-ray1.60A/B807-975[»]
    2W1SX-ray1.45A/B807-975[»]
    2W1UX-ray2.00A/B/C/D807-975[»]
    2WDBX-ray2.03A/B/C/D807-975[»]
    ProteinModelPortaliP26831.
    SMRiP26831. Positions 1498-1628.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26831.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini781 – 953173F5/8 type CPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 F5/8 type C domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG69445.
    HOGENOMiHOG000113726.
    KOiK01197.
    OMAiHTEYAEV.
    OrthoDBiEOG6QVRC1.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    2.60.120.260. 3 hits.
    3.30.379.10. 1 hit.
    InterProiIPR011496. Beta-N-acetylglucosaminidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR000421. Coagulation_fac_5/8-C_type_dom.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR011490. FIVAR_dom.
    IPR008979. Galactose-bd-like.
    IPR017853. Glycoside_hydrolase_SF.
    IPR015882. HEX_bac_N.
    [Graphical view]
    PfamiPF00754. F5_F8_type_C. 2 hits.
    PF07554. FIVAR. 3 hits.
    PF02838. Glyco_hydro_20b. 1 hit.
    PF07555. NAGidase. 1 hit.
    [Graphical view]
    SMARTiSM00231. FA58C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 5 hits.
    SSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS50022. FA58C_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26831-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKNIRKIIT STVLAAMTIS VLPSNLVVFA TDGITENFYE IYPKPQEISY     50
    SGGEFQISDE INIVYDDGID TYTKKRVDEV LEASNLEATV SNEIVPGKTN 100
    FLVGINESGG VVDNYFNKNI PHDESFFDEK MDANIVSVKD GVIGVIGEDT 150
    DSAFYGVTTL KHVFNQLEEG NKIQSFRADD YAEVAHRGFI EGYYGNPWSN 200
    EDRAELMKFG GDYKLNQYVF APKDDPYHNS KWRDLYPEEK LSEIKKLAQV 250
    GNETKNRYVY ALHPFMNNPV RFDTEENYQN DLGVIKAKFT QLLENDVRQF 300
    AILADDASAP AQGASMYVKL LTDLTRWLEE QQSTYPDLKT DLMFCPSDYY 350
    GNGSSAQLKE LNKAEDNVSI VMTGGRIWGE VDENFANNFM NNISTEGHPG 400
    RAPFFWINWP CSDNSKQHLI MGGNDTFLHP GVDPSKIDGI VLNPMQQAEA 450
    NKSALFAIAD YAWNIWDNKE EADENWNDSF KYMDHGTAEE TNSSLALREI 500
    SKHMINQNMD GRVRPLQESV ELAPKLEAFK QKYDSGASIK EDALELIAEF 550
    TNLQKAADYY KNNPGNERTR DQIIYWLNCW EDTMDAAIGY LKSAIAIEEG 600
    DDEAAWANYS EAQGAFEKSK TYGFHYVDHT EYAEVGVQHI VPFIKSMGQN 650
    LSVVIGSIVD PNRIIATYIS NRQDAPTGNP DNIFDNNAST ELVYKNPNRI 700
    DVGTYVGVKY SNPITLNNVE FLMGANSNPN DTMQKAKIQY TVDGREWIDL 750
    EEGVEYTMPG AIKVENLDLK VRGVRLIATE ARENTWLGVR DINVNKKEDS 800
    NSGVEFNPSL IRSESWQVYE GNEANLLDGD DNTGVWYKTL NGDTSLAGEF 850
    IGLDLGKEIK LDGIRFVIGK NGGGSSDKWN KFKLEYSLDN ESWTTIKEYD 900
    KTGAPAGKDV IEESFETPIS AKYIRLTNME NINKWLTFSE FAIISDELEN 950
    AGNKENVYTN TELDLLSLAK EDVTKLIPTD DISLNHGEYI GVKLNRIKDL 1000
    SNINLEISND TGLKLQSSMN GVEWTEITDK NTLEDGRYVR LINTSNEAVN 1050
    FNLTKFEVNS NEVYEPSLVD AYVGDDGAKK AVDGDLKTRV KFLGAPSTGD 1100
    TIVYDLGQEI LVDNLKYVVL DTEVDHVRDG KIQLSLDGET WTDAITIGDG 1150
    VENGVDDMFS TPLKNGYKHG NQSGGIVPID SAYVEGDNLN QKARYVRILF 1200
    TAPYRHRWTV INELMINNGE YISTVNDPTY ISNPIEERGF APSNLRDGNL 1250
    TTSYKPNTNN GEISEGSITY RLSEKTDVRK VTIVQSGSSI SNAKVMARVG 1300
    DGSENVTDQW VQLGTLSNSL NEFINRDYNN IYEIKIEWTD VAPNIYEIIT 1350
    LNQEFEFPVN DSLKAKYDEL INLSGDEYTL SSFETLKEAL NEAKSILDDS 1400
    NSSQKKIDKA LEKLNKAEER LDLRATDFED FNKVLTLGNS LVEEEYTAES 1450
    WALFSEVLEA ANEANKNKAD YTQDQINQIV IDLDASIKAL VKETPEVDKT 1500
    NLGELINQGK SLLDESVEGF NVGEYHKGAK DGLTVEINKA EEVFNKEDAT 1550
    EEEINLAKES LEGAIARFNS LLIEESTGDF NGNGKIDIGD LAMVSKNIGS 1600
    TTNTSLDLNK DGSIDEYEIS FINHRILN 1628
    Length:1,628
    Mass (Da):182,475
    Last modified:January 31, 2002 - v2
    Checksum:iD4252A2512BBED69
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti147 – 1471G → A in strain: CPN50.
    Natural varianti172 – 1754KIQS → EIKN in strain: CPN50.
    Natural varianti250 – 2501V → M in strain: CPN50.
    Natural varianti548 – 5481A → E in strain: CPN50.
    Natural varianti558 – 5581D → E in strain: CPN50.
    Natural varianti614 – 6141G → S in strain: CPN50.
    Natural varianti944 – 9441I → V in strain: CPN50.
    Natural varianti950 – 9501N → S in strain: CPN50.
    Natural varianti979 – 9791T → I in strain: CPN50.
    Natural varianti982 – 9821I → L in strain: CPN50.
    Natural varianti1042 – 10421I → F in strain: CPN50.
    Natural varianti1043 – 1628586Missing in strain: CPN50.
    Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M81878 Genomic DNA. Translation: AAA23259.1.
    BA000016 Genomic DNA. Translation: BAB79897.1.
    PIRiS43904.
    RefSeqiNP_561107.1. NC_003366.1.
    WP_011009663.1. NC_003366.1.

    Genome annotation databases

    EnsemblBacteriaiBAB79897; BAB79897; BAB79897.
    GeneIDi988432.
    KEGGicpe:CPE0191.
    PATRICi19494262. VBICloPer59675_0248.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M81878 Genomic DNA. Translation: AAA23259.1 .
    BA000016 Genomic DNA. Translation: BAB79897.1 .
    PIRi S43904.
    RefSeqi NP_561107.1. NC_003366.1.
    WP_011009663.1. NC_003366.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JNK NMR - A 1498-1628 [» ]
    2OZN X-ray 1.60 B 1498-1628 [» ]
    2W1Q X-ray 1.60 A/B 807-975 [» ]
    2W1S X-ray 1.45 A/B 807-975 [» ]
    2W1U X-ray 2.00 A/B/C/D 807-975 [» ]
    2WDB X-ray 2.03 A/B/C/D 807-975 [» ]
    ProteinModelPortali P26831.
    SMRi P26831. Positions 1498-1628.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-46268N.
    STRINGi 195102.CPE0191.

    Protein family/group databases

    CAZyi CBM32. Carbohydrate-Binding Module Family 32.
    GH84. Glycoside Hydrolase Family 84.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB79897 ; BAB79897 ; BAB79897 .
    GeneIDi 988432.
    KEGGi cpe:CPE0191.
    PATRICi 19494262. VBICloPer59675_0248.

    Phylogenomic databases

    eggNOGi NOG69445.
    HOGENOMi HOG000113726.
    KOi K01197.
    OMAi HTEYAEV.
    OrthoDBi EOG6QVRC1.

    Enzyme and pathway databases

    BioCyci CPER195102:GJFM-216-MONOMER.
    BRENDAi 4.2.2.1. 1503.

    Miscellaneous databases

    EvolutionaryTracei P26831.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    2.60.120.260. 3 hits.
    3.30.379.10. 1 hit.
    InterProi IPR011496. Beta-N-acetylglucosaminidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR000421. Coagulation_fac_5/8-C_type_dom.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR011490. FIVAR_dom.
    IPR008979. Galactose-bd-like.
    IPR017853. Glycoside_hydrolase_SF.
    IPR015882. HEX_bac_N.
    [Graphical view ]
    Pfami PF00754. F5_F8_type_C. 2 hits.
    PF07554. FIVAR. 3 hits.
    PF02838. Glyco_hydro_20b. 1 hit.
    PF07555. NAGidase. 1 hit.
    [Graphical view ]
    SMARTi SM00231. FA58C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 5 hits.
    SSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS50022. FA58C_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens."
      Canard B., Garnier T., Saint-Joanis B., Cole S.T.
      Mol. Gen. Genet. 243:215-224(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CPN50.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 13 / Type A.

    Entry informationi

    Entry nameiNAGH_CLOPE
    AccessioniPrimary (citable) accession number: P26831
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 31, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    The partially purified protein from strain CPN50 is approximately 70 kDa smaller than the sequence indicated here.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3