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P26829 (DHNA_BACYN) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase

EC=1.6.99.3
Alternative name(s):
Alkyl hydroperoxide reductase
Gene names
Name:ahpF
Synonyms:ndh
OrganismBacillus sp. (strain YN-1)
Taxonomic identifier72581 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length519 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activity

NADH + acceptor = NAD+ + reduced acceptor.

Cofactor

Binds 1 FAD per subunit By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 519519NADH dehydrogenase
PRO_0000166788

Regions

Nucleotide binding210 – 24132FAD By similarity
Nucleotide binding349 – 37931NAD By similarity
Nucleotide binding469 – 47911FAD By similarity
Region1 – ?183183Membrane-binding
Region?184 – 519336Catalytic

Amino acid modifications

Disulfide bond337 ↔ 340Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
P26829 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 054FA461B5A156C2

FASTA51955,830
        10         20         30         40         50         60 
MVLEPQIKSQ LNQYLQLMEG DVLLKVSAGN DKVSEDMLSL VDELASMSSR ITVEKTNLER 

        70         80         90        100        110        120 
TPSFSVNRPG EDTGIVFAGI PLGHEFTSLV LALLQVSGRA PKAEQNVIDQ IKNIEGEYHF 

       130        140        150        160        170        180 
ESYISLSCQN CPDVVQALNL MSVLNPGISH TMIDGAAFKD EVESKDIMAV PTVYLNGESF 

       190        200        210        220        230        240 
TSGRMTVEEI LAQLGSGPDA SELADKDPFD VLVVGGGPAG ASSAIYAARK GIRTGIVADR 

       250        260        270        280        290        300 
FGGQIMDTLS IENFISQKYT EGPKLAASLE EHVKEYDIDV MKLQRAKRLE KKDLIEIELE 

       310        320        330        340        350        360 
NGAVLKSKSV ILSTGARWRN VGVPGEQEFK NKGVAYCPHC DGPLFEGKDV AVIGGGNSGV 

       370        380        390        400        410        420 
EAAIDLAGIV NHVTVLEFMP ELKADEVLQE RLNSLPNVTV IKNAQTKEIT GDDKVNGISY 

       430        440        450        460        470        480 
MDRDTEEVHH IELAGVFVQI GLVPNTDWLD GTLERNRFGE IVVDSHGATN VPGVFAAGDC 

       490        500        510 
TNSAYKQIII SMGSGATAAL GAFDYLIRNT TPAESAAAK 

« Hide

References

[1]"Nucleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp. strain YN-1."
Xu X., Koyama N., Cui M., Yamagishi A., Nosoh Y., Oshima T.
J. Biochem. 109:678-683(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Tryptic digestion of NADH dehydrogenase from alkalophilic Bacillus."
Xu X., Kanaya S., Koyama N., Sekiguchi T., Nosoh Y., Ohashi S., Tsuda K.
J. Biochem. 105:626-632(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10701 Genomic DNA. Translation: BAA01545.1.

3D structure databases

ProteinModelPortalP26829.
SMRP26829. Positions 209-511.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.30.10. 2 hits.
InterProIPR012081. Alkyl_hydroperoxide_Rdtase_suF.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR002109. Glutaredoxin.
IPR008255. Pyr_nucl-diS_OxRdtase_2_AS.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF13192. Thioredoxin_3. 1 hit.
[Graphical view]
PIRSFPIRSF000238. AhpF. 1 hit.
PRINTSPR00368. FADPNR.
PR00469. PNDRDTASEII.
SUPFAMSSF52833. SSF52833. 2 hits.
TIGRFAMsTIGR03140. AhpF. 1 hit.
PROSITEPS51354. GLUTAREDOXIN_2. 1 hit.
PS00573. PYRIDINE_REDOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHNA_BACYN
AccessionPrimary (citable) accession number: P26829
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 16, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families