Reviewed, UniProtKB/Swiss-Prot P26829 (DHNA_BACYN)

Names and origin

Protein names

Recommended name:
    NADH dehydrogenase
    EC=1.6.99.3
Alternative name(s):
    Alkyl hydroperoxide reductase

Gene names

Name:	ahpF
Synonyms:	ndh

Organism

Bacillus sp. (strain YN-1)

Taxonomic identifier

72581 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	NADH dehydrogenase		PRO_0000166788
Regions
	Nucleotide binding	210 – 241	32	FAD By similarity
	Nucleotide binding	349 – 379	31	NAD By similarity
	Nucleotide binding	469 – 479	11	FAD By similarity
	Region	1 – ?183	183	Membrane-binding
	Region	?184 – 519	336	Catalytic
Amino acid modifications
	Disulfide bond	337 ↔ 340		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26829-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 054FA461B5A156C2	FASTA	519	55,830
	10 20 30 40 50 60 MVLEPQIKSQ LNQYLQLMEG DVLLKVSAGN DKVSEDMLSL VDELASMSSR ITVEKTNLER 70 80 90 100 110 120 TPSFSVNRPG EDTGIVFAGI PLGHEFTSLV LALLQVSGRA PKAEQNVIDQ IKNIEGEYHF 130 140 150 160 170 180 ESYISLSCQN CPDVVQALNL MSVLNPGISH TMIDGAAFKD EVESKDIMAV PTVYLNGESF 190 200 210 220 230 240 TSGRMTVEEI LAQLGSGPDA SELADKDPFD VLVVGGGPAG ASSAIYAARK GIRTGIVADR 250 260 270 280 290 300 FGGQIMDTLS IENFISQKYT EGPKLAASLE EHVKEYDIDV MKLQRAKRLE KKDLIEIELE 310 320 330 340 350 360 NGAVLKSKSV ILSTGARWRN VGVPGEQEFK NKGVAYCPHC DGPLFEGKDV AVIGGGNSGV 370 380 390 400 410 420 EAAIDLAGIV NHVTVLEFMP ELKADEVLQE RLNSLPNVTV IKNAQTKEIT GDDKVNGISY 430 440 450 460 470 480 MDRDTEEVHH IELAGVFVQI GLVPNTDWLD GTLERNRFGE IVVDSHGATN VPGVFAAGDC 490 500 510 TNSAYKQIII SMGSGATAAL GAFDYLIRNT TPAESAAAK

References

[1]	"Nucleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp. strain YN-1." Xu X., Koyama N., Cui M., Yamagishi A., Nosoh Y., Oshima T. J. Biochem. 109:678-683(1991) [PubMed: 1917890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Tryptic digestion of NADH dehydrogenase from alkalophilic Bacillus." Xu X., Kanaya S., Koyama N., Sekiguchi T., Nosoh Y., Ohashi S., Tsuda K. J. Biochem. 105:626-632(1989) [PubMed: 2760020] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10701 Genomic DNA. Translation: BAA01545.1.
3D structure databases
HSSP	HSSP built from PDB template 1FL2 based on UniProtKB P35340.
ModBase	Search...
Family and domain databases
InterPro	IPR012081. Alkyl_hydroperoxide_Rdtase_suF. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR002109. Glutaredoxin. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PIRSF	PIRSF000238. AhpF. 1 hit.
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03140. AhpF. 1 hit.
PROSITE	PS51354. GLUTAREDOXIN_2. 1 hit. PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHNA_BACYN

Accession

Primary (citable) accession number: P26829

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)