P26823 (DNAK_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein DnaK

Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70

Gene names

Name:	dnaK
Ordered Locus Names:	CPE2033

Organism

Clostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195102 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium perfringens

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a chaperone By similarity.
Induction	By stress conditions e.g. heat shock By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Chaperone protein DnaK HAMAP-Rule MF_00332		PRO_0000078450
Amino acid modifications
	Modified residue	175	1	Phosphothreonine; by autocatalysis By similarity
Experimental info
	Sequence conflict	86 – 87	2	YT → LS in CAA44698. Ref.1
	Sequence conflict	143	1	R → T in CAA44698. Ref.1
	Sequence conflict	218 – 219	2	AQ → GE in CAA44698. Ref.1
	Sequence conflict	237 – 239	3	AAE → GRQ in CAA44698. Ref.1
	Sequence conflict	398	1	F → L in CAA44698. Ref.1
	Sequence conflict	543	1	Missing in CAA44698. Ref.1
	Sequence conflict	554	1	E → Q in CAA44698. Ref.1
	Sequence conflict	589 – 590	2	NN → II in CAA44698. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P26823 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: 88990A19444079BE	FASTA	619	66,482
	10 20 30 40 50 60 MSKIIGIDLG TTNSCVAVME GGEPVVITNS EGARTTPSVV SFQANGERLV GQVAKRQAIT 70 80 90 100 110 120 NPDKTIMSIK RHMGTDYKVN IDGKDYTPQE ISAMILQKLK ADAEAYLGEK VTEAVITVPA 130 140 150 160 170 180 YFNDAERQAT KDAGRIAGLD VKRIINEPTA ASLAYGLDKM DSAHKILVYD LGGGTFDVSI 190 200 210 220 230 240 LDLGDGVFEV VSTNGDARLG GDDFDQRIID YIAEDFKAQN GIDLRQDKMA LQRLKEAAEK 250 260 270 280 290 300 AKIELSSSTQ TLINLPFITA DATGPKHIDM TLTRAKFNEL THDLVERTIN IMKEALKSGN 310 320 330 340 350 360 VSLNDIDKVI LVGGSTRIPA VQEAVKNFTG KEPSKGVNPD ECVAMGAAIQ AGVLTGDVKD 370 380 390 400 410 420 VLLLDVTPLT LGIETLGGVA TPLIERNTTI PARKSQIFST AADNQTSVEI HVVQGERQMA 430 440 450 460 470 480 ADNKTLGRFT LSGIAPAPRG IPQIEVAFDI DANGIVKVSA TDKATGKEAN ITITASTNLS 490 500 510 520 530 540 DAEIDKAVKE AEQFAEEDKK RKEAIEVKNN AEQTVYQTEK TLNELGDKVS AEEKSEIEAK 550 560 570 580 590 600 IEEVKKVKDG DDIEAIKKAM EDLTQAFYKV SEKLYQQNGG AQGQGFDPNN MGGANAGAGA 610 TNNNDDNVVD ADFEVQDDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of HSP70 (dnaK) gene from Clostridium perfringens using a general polymerase chain reaction based approach." Galley K.A., Singh B., Gupta R.S. Biochim. Biophys. Acta 1130:203-208(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62915 Genomic DNA. Translation: CAA44698.1. BA000016 Genomic DNA. Translation: BAB81739.1.
PIR	S22355.
RefSeq	NP_562949.1. NC_003366.1.
3D structure databases
ProteinModelPortal	P26823.
SMR	P26823. Positions 1-507.
ModBase	Search...
Protein-protein interaction databases
STRING	195102.CPE2033.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB81739; BAB81739; BAB81739.
GeneID	990348.
KEGG	cpe:CPE2033.
PATRIC	19498035. VBICloPer59675_2107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0443.
HOGENOM	HOG000228136.
KO	K04043.
OMA	ADGQQGA.
ProtClustDB	PRK00290.
Enzyme and pathway databases
BioCyc	CPER195102:GJFM-2087-MONOMER.
Family and domain databases
HAMAP	MF_00332. DnaK.
InterPro	IPR012725. Chaperone_DnaK. IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 2 hits. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
TIGRFAMs	TIGR02350. prok_dnaK. 1 hit.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAK_CLOPE

Accession

Primary (citable) accession number: P26823

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 31, 2002
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program