P26819 (ARBK2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-adrenergic receptor kinase 2
Short name=Beta-ARK-2
EC=2.7.11.15

Alternative name(s):
G-protein-coupled receptor kinase 3

Gene names

Name:	Adrbk2
Synonyms:	Grk3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.
Catalytic activity	ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	desensitization of G-protein coupled receptor protein signaling pathway Inferred by curator Ref.1. Source: RGD
Cellular component	axon Inferred from direct assay Ref.1. Source: RGD dendritic shaft Inferred from direct assay Ref.1. Source: RGD dendritic spine Inferred from direct assay Ref.1. Source: RGD nucleus Inferred from direct assay. Source: RGD synapse Inferred from direct assay Ref.1. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from direct assay Ref.1. Source: RGD beta-adrenergic receptor kinase activity Inferred from direct assay Ref.1. Source: RGD signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Beta-adrenergic receptor kinase 2		PRO_0000085633
Regions
	Domain	54 – 175	122	RGS
	Domain	191 – 453	263	Protein kinase
	Domain	454 – 521	68	AGC-kinase C-terminal
	Domain	558 – 652	95	PH
	Nucleotide binding	197 – 205	9	ATP By similarity
	Region	1 – 190	190	N-terminal
Sites
	Active site	317	1	Proton acceptor By similarity
	Binding site	220	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26819 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: B4A077F4B48DDD3A	FASTA	688	79,887
	10 20 30 40 50 60 MADLEAVLAD VSYLMAMEKS KATPAARASK KVVLPEPSIR SVMQRYLAER NEITFDKIFN 70 80 90 100 110 120 QKIGFLLFKD FCLNEIGEAV PQVKFYEEIK EYEKLDNEED RLHRSRQMYD AYIMRELLSS 130 140 150 160 170 180 THQFSKQAVE HVQSHLSKKQ VTPTLFQPYI EEICESLRGD IFQKFMESEK FTRFCQWKNV 190 200 210 220 230 240 ELNIHLSMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRVKMK QGETLALNER 250 260 270 280 290 300 IMLSLVSTGD CPFIVCMTYA FHTPDKLCFI LDLMNGGDMH YHLSQHGVFS EKEMRFYASE 310 320 330 340 350 360 IILGLEHMHT CFVVYRDLKP ANILLDEYGH VRISDLGLAC DFSKKKPHAS VGTHGYMAPE 370 380 390 400 410 420 VLQKGTCYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTVN VQLPDAFSPE 430 440 450 460 470 480 LRSLLEGLLQ RDVSQRLGCY GGGARELKEH IFFKGIDWQY VYLRKYPPPL IPPRGEVNAA 490 500 510 520 530 540 DAFDIGSFDE EDTKGIKLLD CDQDLYKNFP LMISERWQQE VVETIYDAVN AETDKIEARK 550 560 570 580 590 600 KAKNKQLCQE EDYAMGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL 610 620 630 640 650 660 LTMEQIMSVE ETQIKDRKCI LLRVKGGKQF VLQCESDPEF AQWLKELTCT FNEAQRLLRR 670 680 APKFLNKPRA AILEFSKPPL CHRNSSGL

References

[1]	"The G-protein-coupled receptor kinases beta ARK1 and beta ARK2 are widely distributed at synapses in rat brain." Arriza J.L., Dawson T.M., Simerly R.B., Martin L.J., Caron M.G., Snyder S.H., Lefkowitz R.J. J. Neurosci. 12:4045-4055(1992) [PubMed: 1403099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M87855 mRNA. Translation: AAA40803.1.
IPI	IPI00196988.
PIR	I73628.
RefSeq	NP_037029.1. NM_012897.2.
UniGene	Rn.9861.
3D structure databases
ProteinModelPortal	P26819.
SMR	P26819. Positions 28-667.
ModBase	Search...
PTM databases
PhosphoSite	P26819.
Proteomic databases
PRIDE	P26819.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25372.
KEGG	rno:25372.
Organism-specific databases
CTD	157.
RGD	2063. Adrbk2.
Phylogenomic databases
HOVERGEN	HBG050559.
Enzyme and pathway databases
BRENDA	2.7.11.15. 5301.
Gene expression databases
Genevestigator	P26819.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K00910.
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00233. PH. 1 hit. SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606389.

Entry information

Entry name

ARBK2_RAT

Accession

Primary (citable) accession number: P26819

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 16, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program