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Protein

Beta-adrenergic receptor kinase 2

Gene

Adrbk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Catalytic activityi

ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATPPROSITE-ProRule annotation
Active sitei317 – 3171Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • beta-adrenergic receptor kinase activity Source: RGD
  • D1 dopamine receptor binding Source: RGD
  • G-protein coupled receptor kinase activity Source: RGD
  • protein serine/threonine kinase activity Source: RGD

GO - Biological processi

  • desensitization of G-protein coupled receptor protein signaling pathway Source: RGD
  • inositol phosphate metabolic process Source: RGD
  • intracellular protein transport Source: RGD
  • multicellular organism aging Source: RGD
  • negative regulation of G-protein coupled receptor protein signaling pathway Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • protein phosphorylation Source: RGD
  • rhodopsin metabolic process Source: RGD
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.15. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-adrenergic receptor kinase 2 (EC:2.7.11.15)
Short name:
Beta-ARK-2
Alternative name(s):
G-protein-coupled receptor kinase 3
Gene namesi
Name:Adrbk2
Synonyms:Grk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2063. Adrbk2.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cilium Source: RGD
  • cytosol Source: RGD
  • dendrite terminus Source: RGD
  • dendritic shaft Source: RGD
  • dendritic spine Source: RGD
  • membrane Source: RGD
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • sperm midpiece Source: RGD
  • synapse Source: RGD
  • Z disc Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688Beta-adrenergic receptor kinase 2PRO_0000085633Add
BLAST

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiP26819.

PTM databases

PhosphoSiteiP26819.

Interactioni

GO - Molecular functioni

  • D1 dopamine receptor binding Source: RGD

Structurei

3D structure databases

ProteinModelPortaliP26819.
SMRiP26819. Positions 28-667.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 175122RGSPROSITE-ProRule annotationAdd
BLAST
Domaini191 – 453263Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini454 – 52168AGC-kinase C-terminalAdd
BLAST
Domaini558 – 65295PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190N-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG050559.
InParanoidiP26819.
KOiK00910.
PhylomeDBiP26819.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26819-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLEAVLAD VSYLMAMEKS KATPAARASK KVVLPEPSIR SVMQRYLAER
60 70 80 90 100
NEITFDKIFN QKIGFLLFKD FCLNEIGEAV PQVKFYEEIK EYEKLDNEED
110 120 130 140 150
RLHRSRQMYD AYIMRELLSS THQFSKQAVE HVQSHLSKKQ VTPTLFQPYI
160 170 180 190 200
EEICESLRGD IFQKFMESEK FTRFCQWKNV ELNIHLSMND FSVHRIIGRG
210 220 230 240 250
GFGEVYGCRK ADTGKMYAMK CLDKKRVKMK QGETLALNER IMLSLVSTGD
260 270 280 290 300
CPFIVCMTYA FHTPDKLCFI LDLMNGGDMH YHLSQHGVFS EKEMRFYASE
310 320 330 340 350
IILGLEHMHT CFVVYRDLKP ANILLDEYGH VRISDLGLAC DFSKKKPHAS
360 370 380 390 400
VGTHGYMAPE VLQKGTCYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH
410 420 430 440 450
EIDRMTLTVN VQLPDAFSPE LRSLLEGLLQ RDVSQRLGCY GGGARELKEH
460 470 480 490 500
IFFKGIDWQY VYLRKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD
510 520 530 540 550
CDQDLYKNFP LMISERWQQE VVETIYDAVN AETDKIEARK KAKNKQLCQE
560 570 580 590 600
EDYAMGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL
610 620 630 640 650
LTMEQIMSVE ETQIKDRKCI LLRVKGGKQF VLQCESDPEF AQWLKELTCT
660 670 680
FNEAQRLLRR APKFLNKPRA AILEFSKPPL CHRNSSGL
Length:688
Mass (Da):79,887
Last modified:August 1, 1992 - v1
Checksum:iB4A077F4B48DDD3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87855 mRNA. Translation: AAA40803.1.
PIRiI73628.
RefSeqiNP_037029.1. NM_012897.2.
UniGeneiRn.9861.

Genome annotation databases

GeneIDi25372.
KEGGirno:25372.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87855 mRNA. Translation: AAA40803.1.
PIRiI73628.
RefSeqiNP_037029.1. NM_012897.2.
UniGeneiRn.9861.

3D structure databases

ProteinModelPortaliP26819.
SMRiP26819. Positions 28-667.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP26819.

Proteomic databases

PRIDEiP26819.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25372.
KEGGirno:25372.

Organism-specific databases

CTDi157.
RGDi2063. Adrbk2.

Phylogenomic databases

HOVERGENiHBG050559.
InParanoidiP26819.
KOiK00910.
PhylomeDBiP26819.

Enzyme and pathway databases

BRENDAi2.7.11.15. 5301.

Miscellaneous databases

PROiP26819.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The G-protein-coupled receptor kinases beta ARK1 and beta ARK2 are widely distributed at synapses in rat brain."
    Arriza J.L., Dawson T.M., Simerly R.B., Martin L.J., Caron M.G., Snyder S.H., Lefkowitz R.J.
    J. Neurosci. 12:4045-4055(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiARBK2_RAT
AccessioniPrimary (citable) accession number: P26819
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 8, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.