P26818 (ARBK2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-adrenergic receptor kinase 2
Short name=Beta-ARK-2
EC=2.7.11.15

Alternative name(s):
G-protein-coupled receptor kinase 3

Gene names

Name:	ADRBK2
Synonyms:	GRK3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.
Catalytic activity	ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.
Tissue specificity	Ubiquitous; brain, spleen > heart, lung > kidney.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: InterPro beta-adrenergic receptor kinase activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Beta-adrenergic receptor kinase 2		PRO_0000085631
Regions
	Domain	54 – 175	122	RGS
	Domain	191 – 453	263	Protein kinase
	Domain	454 – 521	68	AGC-kinase C-terminal
	Domain	558 – 652	95	PH
	Nucleotide binding	197 – 205	9	ATP By similarity
	Region	1 – 190	190	N-terminal
Sites
	Active site	317	1	Proton acceptor By similarity
	Binding site	220	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26818 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 820249EBD827D92F	FASTA	688	79,804
	10 20 30 40 50 60 MADLEAVLAD VSYLMAMEKS KATPAARASK KIVLPEPSIR SVMQKYLEER HEITFDKIFN 70 80 90 100 110 120 QRIGFLLFKD FCLNEINEAV PQVKFYEEIK EYEKLENEED RLCRSRQIYD TYIMKELLSC 130 140 150 160 170 180 SHPFSKQAVE HVQSHLSKKQ VTSTLFQPYI EEICESLRGS IFQKFMESDK FTRFCQWKNV 190 200 210 220 230 240 ELNIHLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 250 260 270 280 290 300 IMLSLVSTGD CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE 310 320 330 340 350 360 IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKKPHAS VGTHGYMAPE 370 380 390 400 410 420 VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTMN VELPDVFSPE 430 440 450 460 470 480 LKSLLEGLLQ RDVSKRLGCH GGSAQELKTH DFFRGIDWQH VYLQKYPPPL IPPRGEVNAA 490 500 510 520 530 540 DAFDIGSFDE EDTKGIKLLD CDQELYKNFP LVISERWQQE VAETVYEAVN ADTDKIEARK 550 560 570 580 590 600 RAKNKQLGHE EDYALGRDCI VHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQSL 610 620 630 640 650 660 LTMEQIVSVE ETQIKDKKCI LLRIKGGKQF VLQCESDPEF VQWKKELTET FMEAQRLLRR 670 680 APKFLNKSRS AVVELSKPPL CHRNSNGL

References

[1]	"Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family." Benovic J.L., Onorato J.J., Arriza J.L., Stone W.C., Lohse M., Jenkins N.A., Gilbert D.J., Copeland N.G., Caron M.G., Lefkowitz R.J. J. Biol. Chem. 266:14939-14946(1991) [PubMed: 1869533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73216 mRNA. Translation: AAA30406.1.
IPI	IPI00716281.
PIR	A39336.
RefSeq	NP_776925.1. NM_174500.2.
UniGene	Bt.4444.
3D structure databases
ProteinModelPortal	P26818.
SMR	P26818. Positions 28-667.
ModBase	Search...
Protein-protein interaction databases
STRING	P26818.
Proteomic databases
PRIDE	P26818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	282136.
KEGG	bta:282136.
Organism-specific databases
CTD	157.
Phylogenomic databases
eggNOG	maNOG08026.
GeneTree	ENSGT00550000074318.
HOVERGEN	HBG050559.
InParanoid	P26818.
OrthoDB	EOG4W3SMD.
Enzyme and pathway databases
BRENDA	2.7.11.15. 908.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K00910.
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00233. PH. 1 hit. SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARBK2_BOVIN

Accession

Primary (citable) accession number: P26818

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 16, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program