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P26817 (ARBK1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-adrenergic receptor kinase 1

Short name=Beta-ARK-1
EC=2.7.11.15
Alternative name(s):
G-protein-coupled receptor kinase 2
Gene names
Name:Adrbk1
Synonyms:Grk2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.

Catalytic activity

ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

Subunit structure

Interacts with GIT1. Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1 By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PH domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 12021252. Source: RGD

aging

Inferred from expression pattern PubMed 15626685. Source: RGD

desensitization of G-protein coupled receptor protein signaling pathway

Inferred from direct assay PubMed 18223549. Source: BHF-UCL

follicle-stimulating hormone signaling pathway

Inferred from direct assay PubMed 16899567. Source: RGD

negative regulation of G-protein coupled receptor protein signaling pathway

Inferred from direct assay Ref.1. Source: RGD

positive regulation of catecholamine secretion

Inferred from direct assay PubMed 18223549. Source: BHF-UCL

protein phosphorylation

Inferred from direct assay Ref.1. Source: RGD

response to hydrogen peroxide

Inferred from expression pattern PubMed 16337875. Source: RGD

response to organic cyclic compound

Inferred from direct assay PubMed 16478977. Source: RGD

response to oxidative stress

Inferred from direct assay PubMed 16478977. Source: RGD

termination of G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapical plasma membrane

Inferred from direct assay PubMed 16899567. Source: RGD

axon

Inferred from direct assay Ref.1. Source: RGD

basolateral plasma membrane

Inferred from direct assay PubMed 16899567. Source: RGD

caveola

Inferred from direct assay PubMed 15626685. Source: RGD

cytoplasm

Inferred from direct assay PubMed 16899567. Source: RGD

dendritic shaft

Inferred from direct assay Ref.1. Source: RGD

dendritic spine

Inferred from direct assay Ref.1. Source: RGD

synapse

Inferred from direct assay Ref.1. Source: RGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

G-protein coupled receptor kinase activity

Inferred from direct assay Ref.1. Source: RGD

beta-adrenergic receptor kinase activity

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689Beta-adrenergic receptor kinase 1
PRO_0000085630

Regions

Domain54 – 175122RGS
Domain191 – 453263Protein kinase
Domain454 – 52168AGC-kinase C-terminal
Domain558 – 65295PH
Nucleotide binding197 – 2059ATP By similarity
Region1 – 190190N-terminal

Sites

Active site3171Proton acceptor By similarity
Binding site2201ATP By similarity

Amino acid modifications

Modified residue6701Phosphoserine By similarity

Experimental info

Sequence conflict24 – 252PA → RR in AAB24228. Ref.2
Sequence conflict461Y → N in AAB24228. Ref.2
Sequence conflict721Y → C in AAB24228. Ref.2
Sequence conflict901E → K in AAB24228. Ref.2
Sequence conflict2491G → V in AAB24228. Ref.2
Sequence conflict2701I → S in AAB24228. Ref.2
Sequence conflict2961F → Y in AAB24228. Ref.2
Sequence conflict4471I → V in AAB24228. Ref.2
Sequence conflict5941D → G in AAB24228. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P26817 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 426A3335ACEB5A34

FASTA68979,785
        10         20         30         40         50         60 
MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR GEVTFEKIFS 

        70         80         90        100        110        120 
QKLGYLLFRD FYLNHLEEAK PLVEFYEEIE KYEKLETEEE RVVRSREIFD SYIMKELLAC 

       130        140        150        160        170        180 
SHPFSKNATE HVQGHLVKKQ VPPDLFQPYI EEICQNLRGD VFHKFIESDK FTRFCQWKNV 

       190        200        210        220        230        240 
ELNIHLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 

       250        260        270        280        290        300 
IMLSLVSTGD CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE 

       310        320        330        340        350        360 
IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKKPHAS VGTHGYMAPE 

       370        380        390        400        410        420 
VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTMA VELPDSFSPE 

       430        440        450        460        470        480 
LRSLLEGLLQ RDVNRRLGCL GRGAQEIKES PFFRSLDWQM VFLQKYPPPL IPPRGEVNAA 

       490        500        510        520        530        540 
DAFDIGSFDE EDTKGIKLLD SDQELYRNFP LTISERWQQE VAETVFDTIN AETDRLEARK 

       550        560        570        580        590        600 
KAKNKQLGHE EDYALGKDCI MHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RGEDEAPQSL 

       610        620        630        640        650        660 
LTMEEIQSVE ETQIKERKCL LLKIRGGKQF VLQCDSDPEL VQWKKELRDA YREAQQLVQR 

       670        680 
VPKMKNKPRS PVVELSKVPL IQRGSANGL 

« Hide

References

[1]"The G-protein-coupled receptor kinases beta ARK1 and beta ARK2 are widely distributed at synapses in rat brain."
Arriza J.L., Dawson T.M., Simerly R.B., Martin L.J., Caron M.G., Snyder S.H., Lefkowitz R.J.
J. Neurosci. 12:4045-4055(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Localization of mRNA for beta-adrenergic receptor kinase in the brain of adult rats."
Owada Y., Watanabe M., Kondo H.
Neurosci. Lett. 144:9-13(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M87854 mRNA. Translation: AAA40802.1.
S48813 mRNA. Translation: AAB24228.1.
PIRI56531.
RefSeqNP_036908.1. NM_012776.1.
UniGeneRn.13010.

3D structure databases

ProteinModelPortalP26817.
SMRP26817. Positions 28-667.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid247278. 2 interactions.
STRING10116.ENSRNOP00000025847.

PTM databases

PhosphoSiteP26817.

Proteomic databases

PRIDEP26817.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25238.
KEGGrno:25238.
UCSCRGD:2062. rat.

Organism-specific databases

CTD156.
RGD2062. Adrbk1.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000006742.
HOVERGENHBG050559.
InParanoidP26817.
KOK00910.
PhylomeDBP26817.

Enzyme and pathway databases

BRENDA2.7.11.15. 5301.

Gene expression databases

GenevestigatorP26817.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605815.
PROP26817.

Entry information

Entry nameARBK1_RAT
AccessionPrimary (citable) accession number: P26817
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families