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P26817

- ARBK1_RAT

UniProt

P26817 - ARBK1_RAT

Protein

Beta-adrenergic receptor kinase 1

Gene

Adrbk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.

    Catalytic activityi

    ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPPROSITE-ProRule annotation
    Active sitei317 – 3171Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. beta-adrenergic receptor kinase activity Source: RGD
    3. G-protein coupled receptor kinase activity Source: RGD
    4. protein binding Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. desensitization of G-protein coupled receptor protein signaling pathway Source: BHF-UCL
    3. follicle-stimulating hormone signaling pathway Source: RGD
    4. G-protein coupled receptor signaling pathway Source: RGD
    5. negative regulation of G-protein coupled receptor protein signaling pathway Source: RGD
    6. positive regulation of catecholamine secretion Source: BHF-UCL
    7. protein phosphorylation Source: RGD
    8. response to hydrogen peroxide Source: RGD
    9. response to organic cyclic compound Source: RGD
    10. response to oxidative stress Source: RGD
    11. termination of G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.15. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-adrenergic receptor kinase 1 (EC:2.7.11.15)
    Short name:
    Beta-ARK-1
    Alternative name(s):
    G-protein-coupled receptor kinase 2
    Gene namesi
    Name:Adrbk1
    Synonyms:Grk2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2062. Adrbk1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. axon Source: RGD
    3. basolateral plasma membrane Source: RGD
    4. caveola Source: RGD
    5. cytoplasm Source: RGD
    6. dendritic shaft Source: RGD
    7. dendritic spine Source: RGD
    8. synapse Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 689689Beta-adrenergic receptor kinase 1PRO_0000085630Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei670 – 6701PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP26817.

    PTM databases

    PhosphoSiteiP26817.

    Expressioni

    Gene expression databases

    GenevestigatoriP26817.

    Interactioni

    Subunit structurei

    Interacts with GIT1. Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi247278. 2 interactions.
    STRINGi10116.ENSRNOP00000025847.

    Structurei

    3D structure databases

    ProteinModelPortaliP26817.
    SMRiP26817. Positions 28-667.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 175122RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini191 – 453263Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini454 – 52168AGC-kinase C-terminalAdd
    BLAST
    Domaini558 – 65295PHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 190190N-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000006742.
    HOVERGENiHBG050559.
    InParanoidiP26817.
    KOiK00910.
    PhylomeDBiP26817.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00233. PH. 1 hit.
    SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26817-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR    50
    GEVTFEKIFS QKLGYLLFRD FYLNHLEEAK PLVEFYEEIE KYEKLETEEE 100
    RVVRSREIFD SYIMKELLAC SHPFSKNATE HVQGHLVKKQ VPPDLFQPYI 150
    EEICQNLRGD VFHKFIESDK FTRFCQWKNV ELNIHLTMND FSVHRIIGRG 200
    GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD 250
    CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE 300
    IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKKPHAS 350
    VGTHGYMAPE VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH 400
    EIDRMTLTMA VELPDSFSPE LRSLLEGLLQ RDVNRRLGCL GRGAQEIKES 450
    PFFRSLDWQM VFLQKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD 500
    SDQELYRNFP LTISERWQQE VAETVFDTIN AETDRLEARK KAKNKQLGHE 550
    EDYALGKDCI MHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RGEDEAPQSL 600
    LTMEEIQSVE ETQIKERKCL LLKIRGGKQF VLQCDSDPEL VQWKKELRDA 650
    YREAQQLVQR VPKMKNKPRS PVVELSKVPL IQRGSANGL 689
    Length:689
    Mass (Da):79,785
    Last modified:August 1, 1992 - v1
    Checksum:i426A3335ACEB5A34
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 252PA → RR in AAB24228. (PubMed:1436718)Curated
    Sequence conflicti46 – 461Y → N in AAB24228. (PubMed:1436718)Curated
    Sequence conflicti72 – 721Y → C in AAB24228. (PubMed:1436718)Curated
    Sequence conflicti90 – 901E → K in AAB24228. (PubMed:1436718)Curated
    Sequence conflicti249 – 2491G → V in AAB24228. (PubMed:1436718)Curated
    Sequence conflicti270 – 2701I → S in AAB24228. (PubMed:1436718)Curated
    Sequence conflicti296 – 2961F → Y in AAB24228. (PubMed:1436718)Curated
    Sequence conflicti447 – 4471I → V in AAB24228. (PubMed:1436718)Curated
    Sequence conflicti594 – 5941D → G in AAB24228. (PubMed:1436718)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87854 mRNA. Translation: AAA40802.1.
    S48813 mRNA. Translation: AAB24228.1.
    PIRiI56531.
    RefSeqiNP_036908.1. NM_012776.1.
    UniGeneiRn.13010.

    Genome annotation databases

    GeneIDi25238.
    KEGGirno:25238.
    UCSCiRGD:2062. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87854 mRNA. Translation: AAA40802.1 .
    S48813 mRNA. Translation: AAB24228.1 .
    PIRi I56531.
    RefSeqi NP_036908.1. NM_012776.1.
    UniGenei Rn.13010.

    3D structure databases

    ProteinModelPortali P26817.
    SMRi P26817. Positions 28-667.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247278. 2 interactions.
    STRINGi 10116.ENSRNOP00000025847.

    PTM databases

    PhosphoSitei P26817.

    Proteomic databases

    PRIDEi P26817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25238.
    KEGGi rno:25238.
    UCSCi RGD:2062. rat.

    Organism-specific databases

    CTDi 156.
    RGDi 2062. Adrbk1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000006742.
    HOVERGENi HBG050559.
    InParanoidi P26817.
    KOi K00910.
    PhylomeDBi P26817.

    Enzyme and pathway databases

    BRENDAi 2.7.11.15. 5301.

    Miscellaneous databases

    NextBioi 605815.
    PROi P26817.

    Gene expression databases

    Genevestigatori P26817.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00233. PH. 1 hit.
    SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The G-protein-coupled receptor kinases beta ARK1 and beta ARK2 are widely distributed at synapses in rat brain."
      Arriza J.L., Dawson T.M., Simerly R.B., Martin L.J., Caron M.G., Snyder S.H., Lefkowitz R.J.
      J. Neurosci. 12:4045-4055(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Localization of mRNA for beta-adrenergic receptor kinase in the brain of adult rats."
      Owada Y., Watanabe M., Kondo H.
      Neurosci. Lett. 144:9-13(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiARBK1_RAT
    AccessioniPrimary (citable) accession number: P26817
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3