Beta-adrenergic receptor kinase 1 - Rattus norvegicus (Rat)

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P26817 (ARBK1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Beta-adrenergic receptor kinase 1
Short name=Beta-ARK-1
EC=2.7.11.15

Alternative name(s):
G-protein-coupled receptor kinase 2

Gene names

Name:	Adrbk1
Synonyms:	Grk2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.
Catalytic activity	ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.
Subunit structure	Interacts with GIT1. Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 15626685. Source: RGD desensitization of G-protein coupled receptor protein signaling pathway Inferred from direct assay PubMed 18223549. Source: BHF-UCL follicle-stimulating hormone signaling pathway Inferred from direct assay PubMed 16899567. Source: RGD positive regulation of catecholamine secretion Inferred from direct assay PubMed 18223549. Source: BHF-UCL response to hydrogen peroxide Inferred from expression pattern PubMed 16337875. Source: RGD response to organic cyclic compound Inferred from direct assay PubMed 16478977. Source: RGD termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 16899567. Source: RGD axon Inferred from direct assay Ref.1. Source: RGD basolateral plasma membrane Inferred from direct assay PubMed 16899567. Source: RGD caveola Inferred from direct assay PubMed 15626685. Source: RGD cytoplasm Inferred from direct assay PubMed 16899567. Source: RGD dendritic shaft Inferred from direct assay Ref.1. Source: RGD dendritic spine Inferred from direct assay Ref.1. Source: RGD synapse Inferred from direct assay Ref.1. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from direct assay Ref.1. Source: RGD beta-adrenergic receptor kinase activity Inferred from direct assay Ref.1. Source: RGD phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 689

689

Beta-adrenergic receptor kinase 1

PRO_0000085630

Regions

Domain

54 – 175

122

RGS

Domain

191 – 453

263

Protein kinase

Domain

454 – 521

AGC-kinase C-terminal

Domain

558 – 652

Nucleotide binding

197 – 205

ATP By similarity

Region

1 – 190

190

N-terminal

Sites

Active site

317

Proton acceptor By similarity

Binding site

220

ATP By similarity

Amino acid modifications

Modified residue

670

Phosphoserine By similarity

Experimental info

Sequence conflict

24 – 25

PA → RR in AAB24228. Ref.2

Sequence conflict

Y → N in AAB24228. Ref.2

Sequence conflict

Y → C in AAB24228. Ref.2

Sequence conflict

E → K in AAB24228. Ref.2

Sequence conflict

249

G → V in AAB24228. Ref.2

Sequence conflict

270

I → S in AAB24228. Ref.2

Sequence conflict

296

F → Y in AAB24228. Ref.2

Sequence conflict

447

I → V in AAB24228. Ref.2

Sequence conflict

594

D → G in AAB24228. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P26817 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 426A3335ACEB5A34
Show »

FASTA

689

79,785

        10         20         30         40         50         60 
MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR GEVTFEKIFS 

        70         80         90        100        110        120 
QKLGYLLFRD FYLNHLEEAK PLVEFYEEIE KYEKLETEEE RVVRSREIFD SYIMKELLAC 

       130        140        150        160        170        180 
SHPFSKNATE HVQGHLVKKQ VPPDLFQPYI EEICQNLRGD VFHKFIESDK FTRFCQWKNV 

       190        200        210        220        230        240 
ELNIHLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 

       250        260        270        280        290        300 
IMLSLVSTGD CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE 

       310        320        330        340        350        360 
IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKKPHAS VGTHGYMAPE 

       370        380        390        400        410        420 
VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTMA VELPDSFSPE 

       430        440        450        460        470        480 
LRSLLEGLLQ RDVNRRLGCL GRGAQEIKES PFFRSLDWQM VFLQKYPPPL IPPRGEVNAA 

       490        500        510        520        530        540 
DAFDIGSFDE EDTKGIKLLD SDQELYRNFP LTISERWQQE VAETVFDTIN AETDRLEARK 

       550        560        570        580        590        600 
KAKNKQLGHE EDYALGKDCI MHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RGEDEAPQSL 

       610        620        630        640        650        660 
LTMEEIQSVE ETQIKERKCL LLKIRGGKQF VLQCDSDPEL VQWKKELRDA YREAQQLVQR 

       670        680 
VPKMKNKPRS PVVELSKVPL IQRGSANGL

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References

[1]	"The G-protein-coupled receptor kinases beta ARK1 and beta ARK2 are widely distributed at synapses in rat brain." Arriza J.L., Dawson T.M., Simerly R.B., Martin L.J., Caron M.G., Snyder S.H., Lefkowitz R.J. J. Neurosci. 12:4045-4055(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Localization of mRNA for beta-adrenergic receptor kinase in the brain of adult rats." Owada Y., Watanabe M., Kondo H. Neurosci. Lett. 144:9-13(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M87854 mRNA. Translation: AAA40802.1. S48813 mRNA. Translation: AAB24228.1.
IPI	IPI00196987.
PIR	I56531.
RefSeq	NP_036908.1. NM_012776.1.
UniGene	Rn.13010.
3D structure databases
ProteinModelPortal	P26817.
SMR	P26817. Positions 28-667.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000025847.
PTM databases
PhosphoSite	P26817.
Proteomic databases
PRIDE	P26817.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25238.
KEGG	rno:25238.
UCSC	RGD:2062. rat.
Organism-specific databases
CTD	156.
RGD	2062. Adrbk1.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000006742.
HOVERGEN	HBG050559.
InParanoid	P26817.
KO	K00910.
OrthoDB	EOG4W3SMD.
Enzyme and pathway databases
BRENDA	2.7.11.15. 5301.
Gene expression databases
ArrayExpress	P26817.
Genevestigator	P26817.
GermOnline	ENSRNOG00000018985. Rattus norvegicus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00233. PH. 1 hit. SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	605815.

Entry information

Entry name

ARBK1_RAT

Accession

Primary (citable) accession number: P26817

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents